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Identifikasi Bakteri Dan Studi Resistensi Antibiotik Enrofloksasin Serta Histopatologi Pada Ikan Lele (Clarias Gariepinus) Di Kabupaten Pasuruan Jawa Timur Rikky Leonard; Umi Utami Dewi; RR Juni Triastuti; Laksmi - Sulmartiwi
Grouper Vol. 14 No. 2 (2023): Grouper: Jurnal Ilmiah Perikanan
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/grouper.v14i2.204

Abstract

Kegiatan budidaya ikan lele tidak terlepas dari berbagai permasalahan salah satunya penyakit. Keberhasilan budidaya ikan lele dipengaruhi oleh beberapa faktor, salah satunya adalah pengendalian serangan penyakit bakteri. Ikan lele rentan terhadap penyakit yang disebabkan oleh infeksi bakteri Aeromonas hydrophila. Bakteri A. hydrophila dapat menyebabkan Motile Aeromonas Septicemia (MAS). Pengendalian penyakit pada ikan lele (Clarias Gariepinus) sering menggunakan antibiotik jenis enrofloxacin. Penggunaan antibiotik dapat menimbulkan dampak negatif salah satunya dapat menimbulkan resistensi.Penelitian ini bertujuan untuk menganalisis jenis bakteri pada ikan lele di Kabupaten Pasuruan dan respon bakteri tersebut terhadap antibiotik enrofloxacin serta memperoleh gambaran histopatologi hati, ginjal dan limpa. Penelitian ini merupakan penelitian deskriptif dan eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL). Sampel terdiri dari 25 sampel ikan lele yang diambil dari 5 kecamatan di Kabupaten Pasuruan. Kecamatan Beji 10 sampel, Kecamatan Rembang 6 sampel, Kecamatan Purwosari 3 sampel, Kecamatan Gempol 3 sampel dan Kabupaten Bangil 3 sampel. Inhibisi bakteri menggunakan 3 perlakuan enrofloxacin yaitu perlakuan A (5 µl), B (7,5 µl) dan C (10 µl). Analisis data menggunakan SPSS seri 25 dengan uji ANOVA dan dilanjutkan dengan uji jarak berganda Duncan.Hasil penelitian menunjukkan bahwa Aeromonas hydrophila teridentifikasi pada 23 sampel dan Aeromonas salmonicida pada 2 sampel. Tidak terdapat resistensi bakteri Aeromonas hydrophila terhadap antibiotik jenis enrofloxacin yang ditunjukkan dengan rata-rata zona hambat terendah pada perlakuan A dosis 5 µl di Kecamatan Purwosari dan rata-rata zona hambat tertinggi diperoleh pada perlakuan C dosis 10 µl di Kecamatan Purwosari. Terjadi kerusakan pada ginjal, hati dan limpa ikan yang diberi Aeromonas hydrophila. Kerusakan pada ginjal meliputi degenerasi, pusat makrofag melano, infiltrasi sel inflamasi, dilatasi pembuluh darah dan kongesti dan nekrosis. Kerusakan pada hati termasuk degenerasi, sinusoid melebar dan kongesti dan nekrosis. Kerusakan limpa termasuk makrofag, perdarahan dan nekrosis. Kesimpulan dari penelitian ini adalah dosis optimal enrofloxacin untuk ikan lele (Clarias gariepinus) di Kabupaten Pasuruan adalah 5 µl.
PEMANFAATAN LIMBAH PERIKANAN SEBAGAI PRODUK NILAI TAMBAH DI PONDOK PESANTREN NURUL HUDA SURABAYA Lailatul Lutfiyah; M Amin Alamsjah; Rr Juni Triastuti; Dwitha Nirmala
Jurnal Abdi Insani Vol 10 No 4 (2023): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v10i4.1243

Abstract

The use of fishery waste emphasizes a cyclical system in the production process, thereby creating clean production. Shrimp waste can pollute the environment around the factory so it needs to be utilized. One of the processing methods that can be done using shrimp heads is to make flavour. Islamic boarding schools and economic independence in an effort to develop Islamic boarding schools - are two things that cannot be separated. Training on fishery waste processing is one method to support economic independence in the form of entrepreneurship in Islamic boarding schools. Processing fishery product waste is an activity carried out in stages, sequentially, cleanly and hygienically, and meets quality requirements in order to convert fishery product waste into value-added products. The target of this community service activity is to establish a solid fishery waste processing business activity at the Nurul Huda Islamic Boarding School in Surabaya and be able to compete in the market continuously. The method used is an approach method that includes, among others, the Participatory method. The results that have been obtained from this activity are an increase in the knowledge of Islamic boarding school students about fishery waste, namely shrimp heads which are used as a flavouring/flavoring ingredient for safe cooking. The activity was attended by 43 students, with educational levels namely middle school and high school. Based on questionnaire data distributed, before the training was carried out, it was found that 93% of the students did not know that shrimp head waste could be used as a flavour. After the training, it was found that 94% of students were interested in making their own flavor production made from shrimp heads.
Analisis Secara Makroanatomi dan Mikroanatomi pada Gonad Ikan Puso (Harpadon nehereus) yang Ditangkap di Perairan Ujung Pangkah dan Perairan Weru, Jawa Timur [Macroscopical and Microscopical Analysis on the Gonad of Bombay Duck Fish (Harpadon nehereus) Caught in Ujung Pangkah and Weru Waters, East Java] Laksmi Sulmartiwi; Rr. Juni Triastuti; Merdeka Agus Saputra
Jurnal Ilmiah Perikanan dan Kelautan Vol. 8 No. 2 (2016): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v8i2.11178

Abstract

Abstrak Survei langsung yang dilakukan pada bulan September menunjukkan bahwa ikan puso merupakan salah satu ikan yang menjadi hasil tangkapan di Perairan Ujung Pangkah dan Perairan Weru. Larangan penangkapan ikan di breeding ground (daerah memijah) dan spawning ground (daerah bertelur) merupakan upaya untuk mencegah terjadinya penangkapan berlebihan (over exploitation). Berdasarkan hal tersebut perlu dilakukannya studi aspek reproduksi ikan puso secara makroanatomi dan mikroanatomi agar dapat dijadikan sebagai dasar untuk mengelola dan mengembangkan ikan puso, sehingga ikan tersebut dapat dipertahankan keberadaanya. Penelitian ini bertujuan untuk mengetahui aspek reproduksi dan tahap perkembangan gonad ikan puso secara makroanatomi dan mikroanatomi pada Perairan Ujung Pangkah dan Perairan Weru. Metode penelitian yang digunakan yaitu metode deskriptif, pada penelitian dibuat deskripsi tentang aspek reproduksi pada gonad ikan puso secara makroanatomi dan mikroanatomi. Berdasarkan hasil penelitian menunjukkan bahwa aspek reproduksi pada gonad ikan puso dari Perairan Ujung Pangkah dan Perairan Weru secara makroanatomi dan mikroanatomi meliputi tahap maturing terjadi pada pada kisaran panjang 28,6-31 cm, tahap mature terjadi pada kisaran panjang 26,1-27,2 cm dan tahap resting terjadi pada panjang 30,5 cm.Tahap perkembangan gonad ikan puso secara makroanatomi pada Perairan Ujung Pangkah dan Perairan Weru meliputi TKG I, TKG II menuju TKG III dan TKG III, sedangkan tahap perkembangan gonad ikan puso secara mikroanatomi pada Perairan Ujung Pangkah dan Perairan Weru meliputi tahap TKG II, TKG III dan TKG V. Abstract The direct surveillance conducted in month of September indicated that bombay duck fish is one of the fish catch yields in Ujung Pangkah and Weru Waters. The prohibition regulation of catching fish in breeding ground and spawning ground is the effort to prevent fish from over-exploitation. Therefore, it requires a research about reproduction aspects study of the bombay duck fish in macroscopical and microscopical way in order to obtain information stood for exploring and developing the bombay duck fish so as the fish can keep sustainable. This research is aimed to identify the reproduction aspects and the development stage of bombay duck fish gonad in macroscopical and microscopical way in Ujung Pangkah and Weru Waters. The research method applied was descriptive method by obtaining the data regarding reproduction aspects of bombay duck fish in macroscopical and microscopical way. As the result, the research showed that the reproduction aspects of bombay duck fish including maturing stage in the range of length between 28,6 cm, mature stage in the range of length between 26,1-27,2 cm and the resting stage occured 30,5 cm. The development of bombay duck fish gonad in macroscopical way in Ujung Pangkah and Weru Waters includes first maturity rate, second maturity rate to third maturity rate and third maturity rate. In contrast, The development of bombay duck fish gonad in microscopical way in Ujung Pangkah and Weru Waters includes second maturity rate, third maturity rate and fifth maturity rate.
Studi Bioakumulasi Timbal (Pb) pada Ikan Bandeng (Chanos chanos Forskall) di Tambak Sekitar Perairan Sungai Buntung, Kabupaten Sidoarjo [ Studies Bioaccumulation of Lead (Pb) in Milkfish (Chanos chanos Forskall) at The Fishponds Around Buntung River, Sidoarjo] Rr. Juni Triastuti; Singgih Aditama; Boedi Setya Rahardja
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 1 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i1.11245

Abstract

Abstract Lead (Pb) has been recognized as hazardous metal because it is commonly found as contaminant, interfere the survival of aquatic organisms, and cause bioaccumulation process in aquatic biota at the waters. Bioaccumulation refers to the accumulation of substances in organism that can trough the food chain reaction of aquatic tissue. Bioaccumulation may occur in milkfish cultivated in fishponds around the Buntung river, Sidoarjo, because there are a lot of factories or industrial activities operating and disposing the waste along the river. The purposes of this study are to measure the lead (Pb) content in milkfish at pond around Buntung river, Sidoarjo, and to determine concentration of water and sediment river, water and sediment of fishponds, klekap, and milkfish meat from fishponds, and also to has exceeded specified maximum limit. Design of this study is descriptive study. The samples were collected from 4 stations that located in Buntung river, Sidoarjo, include water and sediment of river, water and sediment of fishponds, klekap, meats and gills from milkfish. Analyze the lead (Pb) content of water, sediment, and biota used Atomic Absorption Spectrophotometry (AAS). The histopathology slide was made by Haematoxylin-Eosin coloration. Cells of controls are the gill filaments at magnification up to 1000 times. The results showed concentration of lead (Pb) in the river water was about 0,018–0,028 ppm, the sediment of river (2,664–3,072 ppm), the water of fishponds (0,000-0,011 ppm), the sediment of fishponds (2,974-3,220 ppm), klekap (0,176-0,192 ppm), and milkfish meat (0,175-0,190 ppm). The lead concentration of each objects have lower than standardization of quality. The injuries found on the gill filaments of milkfish sample histopathologicaly are cell necrosis, rupture of secondary lamella, and congestion.
Pengaruh Pemberian Minyak Atsiri Daun Bandotan (Ageratum conyzoides) Dalam Transportasi Tertutup Benih Ikan Koi (Cyprinus Carpio) [The Effects Of Bandotan Leave's (Ageratum conyzoides) Essential Oil within Closed System Transportation of Koi Carp (Cyprinus carpio) ] Laksmi Sulmartiwi; Ida Bagus Putu Oka Dantika; Rr. Juni Triastuti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11311

Abstract

Abstract In the ornamental fish business, transportation has an important role to distributing fish up into the costumers. The distance between the farm and the customers location, require a transportation method which can maintain the quality and quantity of ornamental fish during the transportation. The biggest challenge in live fish closed system transportation is to reduce the stress level of fish during the process. According to Harmon (2009), fish are often exposed to multiple stressor during the transportation. Stressed fish can be seen through the increasing of tachiventilation (rapid opercula movement) and blood glucose levels (Evans and Claiborne, 2006). One of the method to reduce stress of fish during transportation is using essential oils from Bandotan's (A. conyzoides) leaves. A. conyzoides essential oils know has an analgesic activity (Okunade, 2002; Shekhar and Anju, 2012; Singh et al., 2013). A chemical compound with known analgesic effect, used in human and veterinary medicine as local analgesic dan be used to fish for the purpose of sedation, immobilization, and analgesia as well as general anaesthesia (Zahl and Samuelsen, 2012). This research was conducted to see the effect of A.conyzoides essential oils toward the survival rate, blood glucose levels, and tachyventilation of koi carp juveniles during closed system transportation. This research using completely randomized design method which consist of 5 different treatments with 4 replications. Treatment that used in this research is the variation of administration doses of A. conyzoides essential oils during the koi carp juvenile closed system transportation. The treatment such as A (5 ppm), B (10 ppm), C (15 ppm), D (20 ppm), and E (0 ppm). Main parameter of this research is survival rate, blood glucose levels, and tachyventilation of koi carp juvenile during 8 hours transportation. The result shows that A. conyzoides essential oils give a significant result toward survival rate, blood glucose levels, and tachiventilation of koi carp juvenile during transportation. There is no mortality in treatment A, B, and E, during transportation. The lowest blood glucose levels and tachiventilation found in treatment A (5 ppm).
Kejadian Kelainan Vertebra Ikan Nila (Oreochromis niloticus) pada Media Penetasan Salinitas yang Berbeda [Vertebrae Malformation Tilapia Fish (Oreochromis niloticus) on Different Media Hatching Saline] Lailatul Lutfiyah; Rr Juni Triastuti; Endang Dewi Masithah; Win Darmanto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11312

Abstract

Abstract Tilapia fish belonged to the euryhaline fish, which has wide range tolerance in salinity (freshwater euryhaline fish has salinity tolerance >8 ppt). Incidence of fish skeletal abnormalities could be the response of the environment influence such as salinity, temperature and hypoxia. This research uses five treatments : 0,5 10, 15, 20 ppt. The results showed that the highest abnormalities found on the treatment of 15 ppt and 20 ppt, while the lowest for the treatment of abnormalities of 10 ppt. The best result to be used in the brackish nila aquaculture is 10-15 ppt in salinity.
Pengaruh Penggunaan Larutan Daun Bandotan (Ageratum conyzoides) terhadap Kadar Glukosa Darah Ikan Koi (Cyprinus carpio) Pasca Transportasi [Influence Use Of Bandotan (Ageratum conyzoides) To Rate Koi Fish (Cyprinus carpio) Blood Glucose After Transportation] Laksmi Sulmartiwi; Sri Harweni; Akhmad Taufiq Mukti; Rr. Juni Triastuti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 1 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i1.11428

Abstract

Abstract Transportation makes stress for fish because of high density and decreasing of water quality. Stress can increase blood glucose. Bandotan have Ca blocking effect. The Ca blocking effect occur in reticulum endoplasmic in smooth muscle. This research aimed to identify bandotan leaf effect in transport medium on blood glucose of koi fish post transportation and to identify best dosage of bandotan leaf on koi fish tranport medium to decrease the blood glucose of koi fish post transportation. Research method used was experimental. Treatment used was providign different bandotan leaf dossage: A (0 g/L), B (0.45 g/L), C (0,9 g/L), D (1.35 g/L) and E (1.8 g/L) and each treatment was repeaetd four times. Main parameter observed in this research was koi fish blood glucose every eight hours for twenty four hours and supporting parameter consisted of: water quality and fish mortality rate. Then the data obtained were prepared using ANOVA, continued by Dunnett Test and then by Duncan's Multiple Distance. Research result shwoed that bandotan leaf supply in transport medium had no effect on koi fish blood glucose post transportation and highest dosage of bandotan leaf in transport medium on blood glucose of koi fish post transportation was 0.45 g/L.
Pengaruh Penambahan Pupuk Bintil Akar Kacang Tanah Sebagai Sumber Nitrogen Dan Fosfor Terhadap Populasi Chlorella sp. [The Effect Of Addition Fertilizer Roots Nodule Peanut As A Source Of Nitrogen And Phosphorus To The Population Of Chlorella sp. ] Rr. Juni Triastuti; A. Shofy Mubarak; Likanimasayu Prabandari
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 2 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i2.11601

Abstract

Abstract Chlorella sp. is one natural food that widely used in hatchery fish, shrimp and oyster. The availability of natural food is a factor that has an important role in farming activities. Culture of Chlorella sp. generally use technical fertilizer (Walne) where nitrogen and phosporus in Walne beginning chemist fertilizer the price expensive. One source of nitrogen and phosphorus naturally obtained from the roots of peanuts. The root nodules contained peanut-containing nodule bacteria Rhizobium are able to bind nitrogen from the air element. By doing immersion can be obtained nutrients to the roots of peanuts are nitrogen and phosphorus that is soluble in water. So that the root nodules of peanuts can be used as a source of nitrogen and phosphorus to increase the population of Chlorella sp. The purpose of this study was to determine the effect of the addition of fertilizer and the concentration of peanut root nodules as a source of nitrogen and phosphorus to the population of Chlorella sp. The research was conducted in June until July in the Laboratory of Education Faculty of Fisheries and Marine Fisheries, Airlangga University, Surabaya. The study design used was Completely Randomized Design (CRD). Materials tested in this study was Chlorella sp. whereas fertilizer used is the root nodules of peanut fertilizer and manure Walne. The concentration of the addition of fertilizer peanut nodule is the treatment A (2.25 ppm), treatment B (4.5 ppm), treatment C (9 ppm) and treatment D (18 ppm). Control treatments using fertilizer Walne 0.5 ml/l (control 1) and 1 ml/l (control 2). The main parameter is observed population density, while supporters of the observed parameter is the measurement of temperature, pH and salinity. The results showed that the addition of fertilizer peanut nodule as a source nitrogen and phosporus influence population of Chlorella sp. The addition of fertilizer best peanut nodule is the treatment B of 1,43755x106 cells/ml on the third day. Water quality parameters during the study remained within the tolerance limit for the growth of Chlorella sp. is pH 7-8, room temperature 29-32 0C range, salinity range between 28-40 ppt and water temperature ranges between 28-30 0C.
Obyek Kesukaan untuk Penempelan Telur (Oviposisi) Ektoparasit Argulus japonicus [ Preference Of Oviposition Object Of Ectoparasite Argulus japonicus] Kismiyati Kismiyati; Nur Maulana Iskhaq; Juni Triastuti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 2 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i2.11646

Abstract

Abstract A. japonicus is ectoparasite that attack only freshwater fish. The fish that was attacked by A. japonicus look skinny, always rub their body and appear some red spot that will manifest for second infection. Prevent and controll of A. japonicus, were used drug or chemical materials like insecticide. This compound can damage the environment and destroy surround ecosystem. Precisely metode to controll A. japonicus was focused for egg stage. The female habits of A. japonicus always lay her eggs (oviposition) on the surface object that surround on they habitat. The research using completely randomized design (CRD). The treatment on this research use 4 oviposition object like rock, wood, plants and PVC pipe. A rock that representative a hard and rough surface. Wood that representative rather solid state and irregular surface, plants with hard surface and representative a life organism (using Echinodorus palaefolius with wide and hard surface leaf), also PVC object that representative a hard and smooth surface. For comparator treatment, all oviposition object was collected in one treatment. All treatment were replicated at four times. Object was placed on bottom aquarium for use as oviposition object, to know preference of oviposition object by oviposition periode and totally eggs produced. ANOVA (analysis of variance) and Tukey-HSD range test were used to analyze data. Results showed that significant effect (p <0,05) on oviposition time and total eggs. The longest oviposition time and the higest eggs total was made by rock and pipe. The fastest oviposition time and the lowest eggs total was made by plants. Water quality during the study in optimum condition for A. japonicus and goldfish , temperature ranges from 26-28 0C, pH 7 and dissolved oxygen (DO) ranged at 5 mg/L.
Pengaruh Persentase Pertukaran Air pada Pertumbuhan Gracilaria verrucosa dalam Budidaya Bak Terkontrol [The Effect Of Percentage Water Replacement On Growth Of Gracilaria verrucosa In Controllable Tanks] Rr. Juni Triastuti; Raindra Daksina; Rochmah kurnijasanti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 1 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i1.11675

Abstract

AbstractGracilaria is a species of red algae which has potentially to culture. Generally, the culture is done in ponds, but today some ponds are use for housing and the pond number are decreasing, and the other hand the water quality of ponds is contaminated by industrial wasted. To overcome these problems, the solution is doing culture in a controllable tanks, no large space is needed, it can do in house, no seasonal influences and the supporting factors like o onutrient, pH, light intensity, temperature (C), salinity (/ ) and water circulation are easily to control. The purpose of oo this research is to determine the effect of water replacement on growth and the total number of chlorophyl a on Gracilaria verrucosa. This research was held at June 19 th 2009 – July 22 th 2009 in Laboratorium Kering, Fakultas Perikanan dan Kelautan and as the comparison is Desa Pulukerto Kecamatan Kraton, Kabupaten Pasuruan, Jawa Timur. The growth measurement in Public Health Veteriner Departement Faculty of Medical Veterinary Airlangga University Surabaya. The chlorophyll a measurement was held in Patology Clinic Departement Faculty of Medical Veterinary Airlangga University Surabaya. This research use Fully Randomized Design method with 5 treatments : in treatment A there was no water replacement (0%), in treatment B the replacement was 25%, replacement in treatment C was 50%, treatment D was 75% and in treatment E, all the water taking out from the tank was 100% replaced by new water from the reservoir, replicated four times. The growth data (heavy and length), with Analysis of Variance (ANOVA) and continueted with Duncan Multiple Range Test.