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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Buletin PSP Jurnal Teknologi Perikanan dan Kelautan Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences JURNAL TEKNOLOGI LINGKUNGAN Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Omni-Akuatika Jurnal Biologi Tropis Jurnal Pengelolaan Perikanan Tropis (Journal Of Tropical Fisheries Management) Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Habitus Aquatica : Journal of Aquatic Resources and Fisheries Management Indonesian Journal of Mollusk COJ (Coastal and Ocean Journal) Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Marine Studies

Nurlisa Alias Butet

Departemen Manajemen Sumberdaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, IPB University, Bogor, 16128, Indonesia

Author-ID : 3299372

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Library & Information Science Social Sciences Veterinary

Published : 42 Documents Claim Missing Document

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Diversity and Abundance of Sand Crabs on the South Coast of Central Java (Diversitas dan Kelimpahan Kepiting Pasir di Pantai Selatan Jawa Tengah) Ali Mashar; Yusli Wardiatno; Mennofatria Boer; Nurlisa A. Butet; Achmad Farajallah
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 19, No 4 (2014): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Kepiting pasir atau undur-undur laut merupakan biota bentik yang hidup di pantai berpasir yang mempunyai nilai ekologi dan nilai ekonomi cukup penting. Adanya tekanan penangkapan mengharuskan adanya pengelolaan yang bijak yang disesuaikan dengan karakteristik populasi kepiting pasir. Informasi tentang jenis dan kelimpahan kepiting pasir penting untuk diketahui terlebih dahulu sebagai langkah awal upaya pengelolaan lestari kepiting pasir. Penelitian ini bertujuan untuk mengetahui komposisi jenis dan kelimpahan setiap jenis kepiting pasir yang terdapat di dua lokasi penelitian, yaitu pantai Bocor, Kabupaten Kebumen, dan pantai Bunton, Kabupaten Cilacap. Hasil penelitian menunjukkan bahwa di kedua lokasi penelitian ditemukan tiga jenis kepiting pasir, yaitu Emerita emeritus, Hippa adactyla, dan Albunea symmysta. Kepiting pasir E. emerita ditemukan dengan persentase komposisi paling besar, 70,5%-75,3%; disusul H. adactyla 22,5%-24,7%; dan A. symmysta 2,2%-4,8%. Kepiting pasir betina dijumpai dengan persentase komposisi paling tinggi, yaitu 78,2% hingga 92,8%. Kepiting pasir E. emeritus juga ditemukan dengan kelimpahan rata-rata paling tinggi di kedua lokasi penelitian, yaitu 5 dan 34 ekor/100m2. Kepiting pasir E. emeritus dan H. adactyla yang ditemukan di pantai Bunton, Cilacap mempunyai kelimpahan lebih tinggi dari yang ditemukan di pantai Bocor, Kebumen, adapun kelimpahan rata-rata Albunea symmysta relatif sama di kedua lokasi penelitian, yaitu sekitar 1 ekor/100m2. Implikasi dari hasil penelitian ini adalah diversitas kepiting pasir di pesisir selatan Jawa Tengah relatif tinggi dengan kelimpahan yang berbeda-beda yang dipengaruhi oleh intensitas aktivitas manusia di wilayah pantai berpasir. Kata kunci: Albunea symmysta, Emerita emeritus, Hippa adactyla, kelimpahan, kepiting pasir, komposisi jenis Sand crabs or mole crabs are benthic fauna that live in the sandy beaches. They have ecological and economic value. Because of fishing pressure to this organism, it requires wise management based on the characteristics of the sand crab population. Information on the type and abundance of sand crabs is important as an initial step in sustainable management of sand crabs. This study aims to determine the species composition and abundance of each species of sand crabs. Sand crab specimens were collected from two study sites, namely Bocor beach, Kebumen, and Bunton beach, Cilacap. The results showed that in both sites found three species of sand crabs, namely Emerita emeritus, Hippa adactyla, and Albunea symmysta. E. emerita found in greatest composition percentage, 70.5% -75.3%; followed by H. adactyla 22.5% -24.7%; and A. symmysta 2.2% -4.8%. Females sand crab found in highest composition percentage, which is 78.2% to 92.8%. E. emeritus were also found in highest abundance average in both sites, 5 and 34 ind.100m-2. E. emeritus and H. adactyla were found on Bunton beach, Cilacap have abundance higher than that found on Bocor beach, Kebumen, while average abundance ofAlbunea symmysta relatively similar in both study sites, which is about 1 ind.100m-2. Implication of this research is high diversity of sand crabs relatively on the southern coast of Central Java with varying abundance that affected by intensity of human activities in the sandy beach area. Keywords: abundance, Albunea symmysta, Emerita emeritus, Hippa adactyla, sand crab, species composition

Keragaman genetik populasi giant snakehead (Channa micropeltes) menggunakan penanda Random Amplified Polymorphic DNA di perairan Taman Nasional Sebangau, Kalimantan Tengah Eva Muhajirah; M Mukhlis Kamal; Nurlisa A. Butet; Arif Wibowo
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 11 No 1 (2021): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.11.1.141-151

Giant Snakehead fish (Channa micropeltes, Cuvier, 1831) also known as Toman is a fish species with good taste and high market value. This study aims to reveal genetic diversity of C. micropeltes population in Sebangau National Park (SNP), Central Kalimantan using molecular analysis with genetic markers Random Amplified Polymorphic DNA (RAPD). The primers used in this study were OPE-07, OPD-03, and OPG-10. The results showed that the polymorphism of C. micropeltes in SNP waters was relatively low (43,1818%) and heterozigosity wal also not much different, i.e 0,2323 and 0,2067. Based on pairwise comparison tests (Fst,), the population of C. micropeltes in SNP waters not significantly different (P>0,05). Thus, it can be concluded that C. micropeltes in SNP waters of the Sebangau and Katingan river populations has no different genetic diversity. Genetically, C. micropeltes in SNP relatively low diversity and low adaptation capability.

Beberapa Aspek Biologi Reproduksi Catfish Predator Indonesia (Wallagonia leerii Bleeker, 1851) di Sungai Sebangau Kalimantan Tengah Nurasiah Riza; Nurlisa Alias Butet; Mohammad Mukhlis Kamal; Arif Wibowo; Okta Simon; Ma'mun Ansori
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol. 11 No. 2 (2021): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Graduate School Bogor Agricultural University (SPs IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.11.2.276-284

Tapah fish (Wallagonia leerii) is one of the economically valuable and popular fish species because its large size and make it the main target for fishing in the Sebangau River. It is feared that excessive exploitation of these fish can cause a decline and threaten the sustainability of tapah fish, which up to now still depend on nature. This research aimed to describe reproductive biology of tapah fish related to sex ratio, gonad maturity, gonado-somato index and fecundity. A total of 96 fish samples were collected using fishing trap and seine net for three months at five sampling sites in Sebangau River. The results showed that the sex ratio of male and female for all samples were 1:0.9 and Chi-square test with a 95% confidence interval showed approaching to 1:1 ratio. The GSI value of male and female ranged from 0,110% – 0,522% and 0,112% – 0,651%. The spawning times were recorded from Desember to January. The total fecundity of 11 female ranged from 2976 to 155501 eggs with an average of 43334 eggs.

Characteristics of Crassostrea Oyster Cytochrome Oxidase Subunit I (Coi) Gene As Species Identity In Delta Cimanuk, West Java Dian Rezki Muliani; Fredinan Yulianda; Nurlisa A Butet
Jurnal Moluska Indonesia Vol. 4 No. 1 (2020): April 2020
Publisher : Masyarakat Moluska Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54115/jmi.v4i1.11

Oysters belong to the Crassostrea, which is a type of shellfish that lives as benthos in waters under the same substrate and environmental conditions will exhibit similar morphological changes in response. There are many types of oysters that have a similar shape are often an obstacle to differentiate of Crassostrea species through morphological identification. The purpose of this research was to identify the types of oysters found in the waters of Delta Cimanuk through analysis of the morphology and nucleotide diversity of Cytochrome Oxidase subunit I (COI) gene, as basic information on proper management and conservation. The morphological identification results show that there were two species of the Crassostrea. Identification of species through the Barcoding DNA technique shows that there is one type of oyster: Crassostrea iredalei with an accuracy of 99.5%.

Concentration of heavy metals (Cd, Hg, and Pb) of amazon sailfin catfish, Pterygoplichthys pardalis (Castelnau, 1855) in Ciliwung River West Java Yuang Dinni Aksari; Dyah Perwitasari; Nurlisa A. Butet
Jurnal Iktiologi Indonesia Vol 15 No 3 (2015): October 2015
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v15i3.61

Amazon armored catfish (Loricariidae) Pterygoplichthys pardalis is abundant in the Ciliwung River and consumed by local people. The present status of the Ciliwung River is polluted by heavy metals and fishes from this river may have the potential hazardous effect on human health. The aim of this study was to analyze the concentration of heavy metals (Cd, Hg, and Pb) in Amazon armored catfish organs from the Ciliwung River. Fish collection and water sampling were conducted in the rainy and dry seasons in three parts of the Ciliwung River, i.e. Bogor (upstream), Depok (middle), and Jakarta (downstream). Heavy metal concentrations were measured by atomic absorption spectrophotometer (AAS). One-way analysis of variance (ANOVA) and Tuckey’s test using R.i386 3.0.0 were performed to access whether heavy metal concentrations varied significantly between location, season, organ and heavy metal. The average of Cd concentrations in the gills, liver,and muscle were 0.000146 ^g.g'1; 0.000828 ^g.g'1; 0.000075 ^g.g'1, respectively. The average of Hg concentrations in the gills, liver, and muscle were 0.002826 ^g g-1; 0.004333 ^g g-1; 0.003960 ^g.g'1 respectively. The average of Pb concentrations in the gills, liver, and muscle were 0.002571 ^g g-1; 0.005467 ^g g-1; 0.001609 ^g.g'1 respectively. The concentration of heavy metals in fish organs were below the permissible limits of the environmental standard of Indonesia and FAO. Heavy metal concentrations in the water were below the baseline of the Indonesia Government Regulation. ANOVA clearly revealed that there was no significant variation of the heavy metal concentrations among the sites and seasons, however the significant difference was found among the organs and type of heavy metal. Local people should be aware because some heavy metals have the tendency to accumulate in various organs of Amazon armored catfish in the Ciliwung River. Abstrak Ikan sapu-sapu (Loricariidae) melimpah di Sungai Ciliwung dan dimanfaatkan sebagai salah satu sumber protein he-wani. Kondisi perairan Sungai Ciliwung ditengarai tercemar logam berat dan jika mengkonsumi ikan sapu-sapu dari sungai ini akan beresiko bagi kesehatan. Penelitian ini bertujuan untuk menganalisis konsentrasi logam berat (Cd, Hg, dan Pb) pada beberapa organ ikan sapu-sapu dari Sungai Ciliwung. Sampel ikan dan air sungai diambil pada musim peng-hujan dan kemarau di tiga lokasi sepanjang Sungai Ciliwung, yaitu Bogor (hulu), Depok (tengah), dan Jakarta (hilir). Konsentrasi logam berat diukur menggunakan spektrofotometer serapan atom (SSA), dianalisis menggunakan uji ANOVA dan dilanjutkan dengan uji Tuckey dengan bantuan program R.i7386 3.0.0. Rata-rata konsentrasi Cd pada in-sang, hati, dan otot ikan berturut-turut 0,000146 ^g.g'1; 0,000828 ^g.g'1; 0,000075 ^g.g'1. Rata-rata konsentrasi Hg pada insang, hati, dan otot ikan berturut-turut 0,002826 ^g.g'1; 0,004333 ^g.g'1; 0,003960 ^g.g'1. Rata-rata konsentrasi Pb pada insang, hati, dan otot ikan berturut-turut 0,002571 ^g.g'1; 0,005467 ^g.g'1; 0,001609 ^g.g'1. Konsentrasi ketiga logam berat pada organ ikan maupun air sungai berada di bawah nilai ambang batas menurut Standar Nasional Indonesia, FAO, maupun Peraturan Pemerintah Republik Indonesia. Uji ANOVA menunjukkan bahwa konsentrasi logam berat pada organ tubuh ikan berdasarkan lokasi dan musim tidak berbeda nyata, namun konsentrasi logam berat antar organ target dan jenis logam berbeda nyata. Perlu kehati-hatian dalam mengkonsumsi ikan sapu-sapu dari Sungai Ciliwung karena beberapa logam berat telah terdeteksi pada organ ikan tersebut.

Population diversity of striped snakehead, Channa striata (Bloch, 1793) from Bekasi, West Java and Barito Kuala, South Kalimantan using Cytochrome B gene Gita Kusuma Rahayu; Dedy Duryadi Solihin; Nurlisa A Butet
Jurnal Iktiologi Indonesia Vol 21 No 1 (2021): February 2021
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v21i1.552

Channa striata or striped snakehead is one of species from family Channidae that widely distributed from India, Southern China to Southeast Asia including Indonesia. It is a commercially important freshwater fish because of its taste and health benefits. High demand of this species trigger many efforts to increase its production, one of them is genetic monitoring. This study used complete Cytochrome b gene sequence of mtDNA for determining genetic variation in wild population of C. striata. C. striata samples (n=31) from two different locations in Indonesia were amplified and analyzed using MEGA ver 7.0. Sequences of 1140 bp complete cyt b gene revealed the presence of 2 haplotypes with 1137 bp conserved sites and 3 bp variable sites (0,26%). Overlapping haplotype was observed in samples from Bekasi, however there were only one haplotype in samples from South Borneo. Interspecies genetic were analysed with species from Genebank and showed that C. striata from Indonesia has close genetic relationships with C. striata from Borneo-Indonesia (MN057164.1) with genetic distance 0%. This study also revealed that C. striata from Indonesia were phylogenetically distinct with C. striata from China with 9,2%K2P genetic distance. Complete cyt b gene has been proven for assessing phylogenetic relationships and population diversity of C. striata in Indonesia. Abstrak Channa striata atau ikan gabus haruan adalah salah spesies dari famili Channidae yang tersebar luas mulai dari India, Cina bagian selatan hingga Asia Tenggara termasuk Indonesia. Hewan ini dikenal sebagai jenis ikan air tawar yang bernilai ekonomis karena rasa dan manfaat kesehatannya. Permintaan yang tinggi akan spesies ini mendorong upaya peningkatkan produksinya salah satunya dari segi pengawasan genetiknya. Penelitian ini menggunakan sekuen utuh gen Cytochrome b (cyt b) pada DNA mitokondria untuk menentukan variasi genetik populasi liar C. striata. Sampel C. striata (n=31) Indonesia asal dua lokasi berbeda berhasil diamplifikasi dan dianalisis menggunakan MEGA ver 7.0. Sekuen utuh gen cyt b sepanjang 1140 bp yang didapat menunjukkan adanya 2 haplotipe dengan 1137 bp situs konservatif dan 3 bp situs bervariasi (0,26%). Haplotipe yang tumpang tindih ditemukan pada sampel asal Bekasi, dan hanya ada satu haplotipe pada sampel asal Kalimantan Selatan. Analisis genetik interspesies dengan spesies dari Genebank menunjukkan sampel C. striata memiliki kedekatan genetik dengan C. striata (MN057164.1) asal Kalimantan-Indonesia dengan jarak genetik 0%. Penelitian ini juga menunjukkan bahwa C. striata dengan C. striata asal Cina dengan rata-rata jarak genetik 9,2%. Gen cyt b utuh mampu menjelaskan kekerabatan filogenetik dan memberikan informasi keragaman populasi C. striata asal Indonesia.

AUTENTIKASI SPESIES IKAN KERAPU BERDASARKAN MARKA GEN MT-COI DARI PERAIRAN PEUKAN BADA, ACEH Mohammad Mukhlis Kamal; Agus Alim Hakim; Nurlisa Alias Butet; Yulia Fitrianingsih; Rika Astuti
Jurnal Biologi Tropis Vol. 19 No. 2 (2019): Juli - Desember
Publisher : Universitas Mataram

Abstrak : Variasi bentuk dan pola pewarnaan tubuh ikan kerapu (Famili Serranidae) sangat variatif, sehingga pengenalan spesies secara morfologis sering tidak akurat. Penelitian ini bertujuan untuk mengautentikasi ikan kerapu dengan menggunakan marka gen COI. Contoh ikan kerapu yang diamati berjumlah 29 ekor yang dikumpulkan dari tiga tempat pendaratan ikan di Perairan Peukan Bada, Propinsi Aceh. Secara karakter morfologis, ikan kerapu tersebut teridentifikasi lebih dari 8 spesies. Untuk analisis DNA, sebanyak 30 mg daging sirip dari setiap ikan contoh diambil untuk dilakukan isolasi dan ekstraksi DNA, kemudian visualisasi elektroforesis dan fragmentasi DNA gen COI dengan metode PCR-sekuensing. Setelah diekstraksi, diperoleh 20 sampel DNA yang tervisualisasi dengan baik, yang dari jumlah tersebut terdapat 16 sampel dapat diamplifikasi. Hasilnya menunjukkan terdapat 6 spesies yang terautentikasi. Kelompok pertama adalah Variola albimarginata, Cephalopholis urodeta, dan C. sexmaculata dengan tingkat kemiripan Ã¢â°Â¥ 97%. Berikutnya C. boenak, Epinephelus merra, dan Scolopsis vosmeri tingkat kemiripannya Ã¢â°Â¤ 97%. Bila dibandingkan hasil autentikasi DNA, hasil penelitian menunjukkan bahwa 13 sampel atau > 80% tidak teridentifikasi dengan benar secara morfologis. Berdasarkan jarak genetik, pohon filogeni membentuk 2 clade antara Serranidae dan Nemipteridae. Hasil penelitian menunjukkan bahwa penggunaan marka gen COI sangat efektif untuk autentikasi spesies yang dapat dijadikan sebagai instrumen dalam pemanfaatan dan pengelolaan ikan kerapu.Kata kunci : kerapu, variasi morfologi, gen MT-COI, autentikasi.Abstract : The groupers (family Serranidae) show high variability both in body shapes and coloration leads to highly morphological-based misidentification. The research was aimed in autenthication of the grouper species using MT-COI gene. A total of 29 grouper fishes were collected from three fish landing sites of Peukan Bada, Aceh Province. These fishes were morphologically identified from which more than 8 species were obtained. A 30 mg of the fin meat of each sample was taken for DNA extraction, isolation, electrophoresis visualization, and DNA fragmentation of COI gene using PCR-sequenching.ÃÂ There were 20 DNA samples was clearly visualized of which 16 has been proceeded for amplification. The results showed that V. albimarginata, C. urodeta, and C. sexmaculata showed Ã¢â°Â¥ 97% similarity, whereas C. boenak, E. merra, dan S. vosmeri with Ã¢â°Â¤ 97% similarity. Based on phylogenetic tree analysis there was 2 clearly different clades separating family of Serranidae and Nemipteridae. The use of MT-COI gene was effective and accurate tool in species authentication which could be used as an instrument for utilization and management of the grouper species.Keywords : groupers, morphological variation, MT-COI gene, autenthication.

Kajian Stok Ikan Teri (Stolephorus commersonnii Lacepede, 1803) Di Teluk Palabuhanratu, Sukabumi, Jawa Barat Inggrid Wahyuni Eviasta; Mennofatria Boer; Nurlisa A Butet
Tropical Fisheries Management Journal Vol 2 No 2 (2018): Jurnal Pengelolaan Perikanan Tropis
Publisher : Departement of Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, IPB University

Ikan teri (Stolephorus commersonnii) merupakan ikan pelagis kecil yang memiliki nilai ekonomis dan ekologis penting. Tujuan penelitian ini adalah menganalisis beberapa parameter dinamika populasi dan status stok ikan teri (Stolephorus commersonnii) di Teluk Palabuhanratu. Penelitian dilakukan pada bulan Mei hingga September 2017 pada periode bulan gelap dengan metode yang digunakan Penarikan Contoh Acak Sederhana (PCAS) dan holistik. Hasil penelitian menunjukkan bahwa ikan teri memiliki pola pertumbuhan alometrik negatif. Berdasarkan analisis model produksi surpus menggunakan model Fox, ikan teri berstatus overexploited. Upaya pengelolaan yang dapat dilakukan adalah pengendalian eksploitasi melalui penerapan alat tangkap ramah lingkungan.

River Order Analysis and Fish Stadia Distribution as a Basis for Protected Areas Determination of Freshwater Eel (Anguilla spp.) in Cimandiri Watershed, West Java: Analisis Orde Sungai Dan Distribusi Stadia Sebagai Dasar Penentuan Daerah Perlindungan Ikan Sidat (Anguilla spp.) Di DAS Cimandiri, Jawa Barat Agus Alim Hakim; Mohammad Mukhlis Kamal; Nurlisa Alias Butet; Ridwan Affandi
Tropical Fisheries Management Journal Vol 3 No 1 (2019): Jurnal Pengelolaan Perikanan Tropis
Publisher : Departement of Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, IPB University

Sungai merupakan habitat makhluk hidup dimana terjadi hubungan timbal balik antara makhluk hidup dengan lingkungannya. Sungai yang merupakan habitat ikan sidat (Anguilla spp.) harus tetap terjaga agar pertumbuhan dan perkembangan tidak terganggu serta keberadaannya tetap lestari. Ikan sidat pada stadia larva akan bermigrasi dari laut ke perairan sungai hingga hulu dan setelah dewasa akan kembali ke laut untuk memijah. Penelitian ini bertujuan untuk menganalisis orde sungai dan mengkaji distribusi stadia ikan sebagai dasar penentuan daerah perlindungan ikan sidat (Anguilla spp.) di DAS Cimandiri, Jawa Barat. Penelitian ini dilakukan pada bulan Desember 2014 dan April 2015 meliputi tracking sungai (pengamatan habitat sungai, pengamatan aktivitas penangkapan, dan wawancara), analisis orde sungai, dan penentuan distribusi tiap stadia ikan sidat. DAS Cimandiri memiliki panjang sebesar 69,5 km dengan komposisi sungai permanen (permanent) dan sungai periodik (intermittent) memiliki orde sungai dari 1 hingga 7. Terdapat 56 sungai permanen bagian DAS Cimandiri yang merupakan sungai dengan orde sungai 1. Sungai dengan orde sungai 1 merupakan bagian hulu yang kondisi habitat dan perairannya masih baik. Beberapa dari bagian sungai yang memiliki orde sungai 1 dapat ditetapkan sebagai daerah perlindungan ikan sidat (Anguilla spp.). Distribusi berdasarkan stadia menunjukkan bahwa ikan sidat memiliki komposisi stadia yang tercampur, tetapi glass eels terdistibusi hanya pada 5 km dari arah laut. Adanya perbedaan stadia dari setiap bagian sungai maka diperlukan pengelolaan yang berbeda pula pada masing-masing bagian sungai tersebut.

Species Validation of Mole Crabs Based on Molecular Marker of 16s rRNA from Bantul and Purworejo waters: Validasi Spesies Undur-Undur Laut Berdasarkan Penanda Molekuler 16s rRNA dari Perairan Bantul dan Purworejo Nurlisa A Butet; Inge Anggraeni Bela Putri Dewi; Zairion Zairion; Agus Alim Hakim
Tropical Fisheries Management Journal Vol 3 No 2 (2019): Jurnal Pengelolaan Perikanan Tropis
Publisher : Departement of Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, IPB University

Mole crabs live in intertidal areas of sandy beaches. Identification of aquatic species is often due misidentification cause by the cryptic phenomenon, so it is needed identification technique with a molecular approach by DNA barcoding. The study was purposed to validity and analyze kinship of the mole crabs based on 16S rRNA molecular marker from Bantul and Purworejo waters. Commercial kit of Gene Aid was used to isolation and extraction of DNA and was produced three total DNA from each location. DNA total with good quality was continued to amplified process using PCR technique with a primer of 16S rRNA. Sequence products of 16S rRNA gene were aligned with several sequences of species other from genus Emerita, Scylla, and Portunus that was found from GenBank. Species validation was performed using BLASTn. Genetic distance and phylogenetic tree were obtained MEGA 5.0 program. The samples of mole crab from Bantul and Purworejo were insignificantly different and the samples were from the same genetic sources.

Co-Authors . Erwyansyah . Zairion ACHMAD FARAJALLAH Agus Alim Hakim Ali Mashar Annisa Putri Nandini Arif Wibowo Arif Wibowo Ayu, Inna Puspa Azizah, Hikmatul Beginer Subhan Benny Setiyadi Borbee, Erin Dedi Soedharma Dedy D. Solihin Dian Rezki Muliani Dietriech Geoffrey Bengen Endah Sri Rahayu Etty Riani Eva Muhajirah Findra, Muhammad Nur Forcep Rio Indaryanto Fredinan Yulianda Gelis, Ester R. E. Gita Kusuma Rahayu Hawis H Madduppa Hemat Sirait Hikmatul Azizah Hikmatul Azizah Husain Latuconsina Inge Anggraeni Bela Putri Dewi Inggrid Wahyuni Eviasta Irianda, Nadya Jeny Isdradjad Setyobudiandi Isdradjad Setyobudiandi Kadarwan Soewardi Kadarwan Soewardi Kismanto Koroy Lane, Christopher Lella Herdiana Luky Adrianto Lusita Meilana M Mukhlis Kamal M Mukhlis Kamal M Mukhlis Kamal M. M. Kamal M. Mukhlis Kamal M.M. Kamal Ma'mun Ansori Majariana Krisanti Mala Nurilmala Maya F Tamimi Mennofatria Boer Mohammad Mukhlis Kamal Muhammad Mukhlis Kamal Muhammad Reza Cordova Mukhlas Ansori Mukhlis Kamal, Muhammad Nurasiah Riza Nurfajar, Nurfajar Nurfajrin Nisa Okta Simon Prasadi, Oto Rahayu, Endah Sri Rahmat Kurnia Ridwan Affandi RIDWAN AFFANDI Ridwan Affandi Rika Astuti Risa Tiuria Rudhy Gustiano Rudhy Gustiano Rugaya H. Serosero Ruzanna, Arina Sani, L. Mukhsin Iqbal Shofirma, Adela Sulistiono Syamsul Bachry TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATI NURHAYATI Thomas Nugroho Wahyu Muzammil Yuang Dinni Aksari Yulia Fitrianingsih Yusli Wardiatno

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