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Morphological and molecular identification of seahorses (Hippocampus spp.) from the coast of Sumatra Island, Indonesia Fadiyah Hanifaturahmah; Dyah Perwitasari-Farajallah; Nurlisa Alias Butet; Mala Nurilmala; Agus Oman Sudrajat; Yoshihiro Ochiai
Biodiversitas Journal of Biological Diversity Vol. 21 No. 9 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210924

Abstract

Abstract. Hanifaturahmah F, Perwitasari-Farajallah D, Butet NA, Nurilmala M, Sudrajat AO, Ochiai Y. 2020. Morphological and molecular identification of seahorses (Hippocampus spp.) from the coast of Sumatra Island, Indonesia. Biodiversitas 21: 4116-4123. Seahorses (Hippocampus spp.) species are difficult to be identified based on their color, shape, and body size, especially for their juveniles. The purposes of this study were to identify seahorse species from Indonesian waters (Riau Islands and Lampung, Sumatra Island) based on the sequence variations in the 16S rRNA and COI genes and to analyze the kinship of these seahorses. The morphological identification was performed, followed by the validation of the identification based on a molecular approach. Both identification methods showed the one species from Riau Islands was H. kuda, while the others (n=2) were H. comes. All specimens from Lampung (n=3) were H. comes. The specimens of H. comes from Riau Islands and Lampung were the same haplotype based on the 16 S rRNA sequence. Based on the COI, H. comes from Riau Islands (n=2) were found to be one haplotype (haplotype 1; n=2), while those from Lampung (n=3) consisted of two haplotypes (haplotype 1; n=2 and haplotype 2; n=1). Phylogenetic analysis based on both genes revealed that H. comes from Riau Islands and Lampung belongs to the same clade, while H. kuda from Riau Islands (n=1) was included in the lineage B (Indian Ocean).
Reproductive characteristics of Keudawah (Rasbora arundinata (Lumbantobing, 2014)): Insights into a native species of Krueng Lanca River, Nagan Raya District, Aceh Province, Indonesia MU’AMAR ABDAN; SULISTIONO SULISTIONO; MOHAMMAD MUKHLIS KAMAL; ALI MASHAR; NURLISA ALIAS BUTET
Biodiversitas Journal of Biological Diversity Vol. 26 No. 3 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260347

Abstract

Abstract. Abdan M, Sulistiono, Kamal MM, Mashar A, Butet NA. 2025. Reproductive characteristics of Keudawah (Rasbora arundinata, Lumbantobing 2014): Insights into a native species of Krueng Lanca River, Nagan Raya District, Aceh Province, Indonesia. Biodiversitas 26: 1451-1463. Keudawah (Rasbora arundinata (Lumbantobing, 2014)) is a freshwater fish native to Indonesia, particularly found in the waters of Nagan Raya. Understanding its reproductive biology is crucial for developing sustainable management and conservation strategies. Given this importance, this study aimed to assess the spawning season, sex ratio, Gonadal Maturity Stage (GMS), size at first gonadal maturity (LM50), and fecundity of R. arundinata in the Krueng Lanca River, Aceh Province, Indonesia. Sampling was conducted from January to December 2023 at six stations selected based on habitat characteristics and local fishermen’s recommendations. Fish were caught using gill nets with mesh sizes of 0.5 and 1.0 inches, as well as throw nets with a 6-meter diameter and a 0.5-inch mesh size during monthly sampling. The collected specimens were measured for total length and weighed, after which their gonads were analyzed to determine maturity stage, Gonadosomatic Index (GSI), and fecundity. The size at first gonadal maturity (LM50) was estimated using the Spearman-Karber method. A total of 2,225 specimens were collected, comprising 1,162 males and 1,063 females, with size distributions ranging from 34 to 121 mm (males) and 46 to 135 mm (females). The length-weight relationship is expressed as W = 8.17E-05L2.55 (R² = 0.88), while in females, it follows the equation W = 8.17E-05L2.11 (R² = 0.89). The results indicated that the sex ratio was slightly male-biased, averaging 1.09:1 (M:F). The LM50 was estimated at 86.98 mm for males, with a Gonadosomatic Index (GSI) of 6.96%-9.10%, and 94.77 mm for females, with a GSI of 10.30%-12.61%. Fecundity of R. arundinata ranged from 1,156 to 2,090 oocytes. The findings suggest that R. arundinata exhibits year-round reproductive activity, with peak spawning occurring in April and September. These insights are valuable for conservation planning and provide a foundation for future aquaculture development.
Sex-Based Morphological Differentiation and Stock Homogeneity of Rasbora arundinata (Lumbantobing, 2014) in the Krueng Lanca River, Aceh, Indonesia Mu'amar Abdan; Sulistiono Sulistiono; Mohammad Mukhlis Kamal; Ali Mashar; Nurlisa Alias Butet; Fazril Saputra; Iwan Hasri
Journal of Aquaculture and Fish Health Vol. 15 No. 3 (2026): JAFH Vol 15 No 3 September 2026
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v15i3.87519

Abstract

Sexual dimorphism and spatial variation in morphometric and meristic traits provide important insights into population structure and biological differentiation in freshwater fishes. This study investigated sexual dimorphism and spatial variation in Rasbora arundinata from the Krueng Lanca River, Aceh, Indonesia. A total of 76 individuals, consisting of 40 males and 36 females, were examined. Twenty-three morphometric and ten meristic characters were measured and analyzed using univariate analysis of variance and principal component analysis. The results showed that only four morphometric characters—body width, pectoral fin base length, pelvic fin base length, and standard length—differed between males and females, while the remaining characters were similar between sexes. Females generally exhibited larger body size and greater body robustness than males, indicating subtle sexual dimorphism mainly associated with size- and fin-related traits. Principal Component Analysis supported these findings by revealing partial separation between male and female individuals, with body width and pectoral fin base length contributing most strongly to overall morphometric variation. Meristic analysis also revealed sex-related differences, particularly in lateral line scale counts and fin ray numbers. Females tended to have higher lateral line scale counts, whereas males showed greater variability in several fin ray characters. Spatial analysis based on sampling stations revealed no clear morphological separation among locations, indicating limited spatial variation and suggesting that R. arundinata in the Krueng Lanca River represents a single, morphologically homogeneous stock. Overall, morphological variation in R. arundinata appears to be driven more strongly by sex-related biological factors than by spatial environmental differences.
Co-Authors . Erwyansyah ACHMAD FARAJALLAH Agus Alim Hakim Agus Oman Sudrajat ALI MASHAR Ali Mashar Ali Mashar Ansori , Mukhlas Ansori, Ma'mun Arif Wibowo Ayu, Inna Puspa Azizah, Hikmatul Beginer Subhan Borbee, Erin Dedi Soedharma Dedy D. Solihin, Dedy D. Dian Rezki Muliani Dietriech Geoffrey Bengen DYAH PERWITASARI -FARAJALLAH Endah Sri Rahayu Etty Riani Eva Muhajirah Fadiyah Hanifaturahmah Fazril Saputra Findra, Muhammad Nur Forcep Rio Indaryanto Fredinan Yulianda Gelis, Ester R. E. Gita Kusuma Rahayu Hawis H Madduppa Hemat Sirait Hikmatul Azizah Hikmatul Azizah Husain Latuconsina Inge Anggraeni Bela Putri Dewi Inggrid Wahyuni Eviasta Irianda, Nadya Jeny Isdradjad Setyobudiandi Isdradjad Setyobudiandi Iwan Hasri Kadarwan Soewardi Kadarwan Soewardi Kismanto Koroy Lane, Christopher Lella Herdiana Luky Adrianto Lusita Meilana M Mukhlis Kamal M Mukhlis Kamal M Mukhlis Kamal, M Mukhlis M. M. Kamal M. Mukhlis Kamal, M. Mukhlis M.M. Kamal Majariana Krisanti Mala Nurilmala Mala Nurilmala Maya F Tamimi Mennofatria Boer Mohammad Mukhlis Kamal Muhammad Mukhlis Kamal Muhammad Mukhlis Kamal Muhammad Reza Cordova, Muhammad Reza Mu’amar Abdan Nandini, Annisa Putri Nurfajar Nurfajar Nurfajrin Nisa Prasadi, Oto Rahayu, Endah Sri Rahmat Kurnia Ridwan Affandi RIDWAN AFFANDI Ridwan Affandi Rika Astuti Risa Tiuria Riza, Nurasiah Rudhy Gustiano Rudhy Gustiano Rugaya H. Serosero Ruzanna, Arina Sani, L. Mukhsin Iqbal Setiyadi, Benny Simon, Okta Sulistiono Sulistiono Sulistiono SULISTIONO SULISTIONO Syamsul Bachry TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATI NURHAYATI Thomas Nugroho Wahyu Muzammil Yoshihiro Ochiai Yuang Dinni Aksari Yulia Fitrianingsih Yusli Wardiatno Zairion