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All Journal HAYATI Journal of Biosciences Saintia Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati agriTECH JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Penelitian Pendidikan IPA (JPPIPA) Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat Jurnal Pembelajaran dan Biologi Nukleus Jurnal Ilmiah Biologi UMA (JIBIOMA)
KIKI NURTJAHJA
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara Jl. Bioteknologi No.1, Kampus USU, Medan, Indonesia 20155
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Physics Social Sciences

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PENGHAMBATAN SERANGAN Sclerotium rolfsii PENYEBAB REBAH KECAMBAH PADA KEDELAI DENGAN BAKTERI KITINOLITIK Novi Malinda; Dwi Suryanto; Kiki Nurtjahja
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (504.203 KB)

Abstract

The inhibition of Sclerotium rolfsii as causal agent of soybean damping off by using chitinolytic bacteria were studied in Laboratory of Observation Pest and Disease UPT-BPTPH 1 and Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan. The aim of this research was to investigate  the ability of chitinolytic bacteria to inhibit the growth of S. rolfsii. Six isolates of chitinolytic bacteria were used in this study. The inhibition zone between colony of the bacteria and the fungus was measured. The result showed that the bacterial isolates has different ability in inhibiting the growth of S. rolfsii. Enterobacter sp. BK15 and Bacillus sp. BK13 were the most effective in inhibiting the growth of S. rolfsii with inhibition zone of 3.70 cm and 3.75 cm, respectively. The less effective was shown by Enterobacter sp. PB17 with inhibition zone of 0.10 cm, while Enterobacter cloacae LK08 have no ability to inhibit S. rolfsii. The seeds soaked in bacterial suspension by  Bacillus sp. BK13 dan Enterobacter sp. BK15 were able to reduce damping off by 44.4% and 50%, respectively.   Keywords: Bacillus sp., damping off, Enterobacter sp., Sclerotium rolfsii, soybean
KEMAMPUAN BAKTERI ENDOFIT TANAMAN SEMANGKA DALAM MENEKAN PERKEMBANGAN PENYAKIT BERCAK DAUN YANG DISEBABKAN OLEH JAMUR COLLETOTRICHUM SP. Eryna Elfasari Rangkuti; Dwi Suryanto .; Kiki Nurtjahja .; Erman Munir .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 2 (2014): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.67 KB) | DOI: 10.23960/j.hptt.214170-177

Abstract

Ability of watermelon endophytic bacteria to suppress development of leaf spot disease caused by Colletotrichum sp. A studi on assay of endophytic bacteria to control Colletotrichum sp., causal agent of leaf spot disease on watermelon, was conducted. Colletotrichum sp. was isolated from infected leaf of leaf spot disease, while endophytic bacteria were isolated from stem, leaf, and root of watermelon healthy plant. Antagonistic assay was conducted by dual culture method. Hyphal abnormalities as a result of antagonistic assay was observed using light microscope. To determine the ability of endophytic bacterial isolates to control leaf spot disease, watermelon seeds were treated by dipping the seed in endophytic bacterial suspension. Seven endophytic bacterial isolates showed to inhibit Colletotrichum sp. to some extent. Two isolates DS 01 and BS 01 showed relatively high inhibition zone compared to others, therefore were choosen for further study. Abnormal hyphae such as broken, lysis, twisted, curled, and swollen hyphae were recorded as the result of antagonistic assay. Watermelon seed treatment revealed that dipping the seed in endophytic bacterial suspension of DS 01 and BS 01 reduced leaf spot disease to 12 and 24%, but inhibited seed growth by 12% and 44%, respectively. It seemed that all treatments showed to reduce seedling performance i.e seedling height, leaf number, and dry weight, compared to that of (-) control. DS 01 however seemed to increase dry weight of watermelon plants.
ASAI KEMAMPUAN BAKTERI ENDOFIT DARI KACANG TANAH DALAM MENGHAMBAT PERTUMBUHAN Sclerotium sp. PADA KECAMBAH KACANG TANAH Lisa Novita Arios; Dwi Suryanto .; Kiki Nurtjahja .; Erman Munir .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 2 (2014): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (886.632 KB) | DOI: 10.23960/j.hptt.214178-186

Abstract

Assay on ability of endophytic bacteria isolated from peanut to inhibit Sclerotium sp. growth in peanut seedlings. A study on assay of ability of endophytic bacteria to inhibit Sclerotium sp. in peanut seedling has been done. The bacteria were isolated from peanut healthy plants, while Sclerotium sp. was isolated from infected peanaut plant. Antagonistic assay was conducted by dual culture method. In vivo assay of inhibiting Sclerotium sp. was conducted by dipping peanut seed in bacterial solution, and planting the seed in soil:compost (3:1) growing media. Six endophytic bacterial isolates showed to inhibit the growth of Sclerotium sp. in vitro. LN1 seemed to inhibit more of Sclerotium sp., while LN5 showed to inhibit less. Two potential isolates LN1 of gram-negative and LN2 of gram-positive using for further study showed to decrease more of dumping off. It also seemed that the isolates increased the seedling height, number of leaves, and dry weight.
Pengaruh Konsentrasi Air Kelapa dan Air Cucian Beras dan Lama Perendaman Terhadap Perkecambahan Benih Kacang Hijau (Vigna radiata L.) Srimaulinda Srimaulinda; Kiki Nurtjahja; Riyanto Riyanto
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 3, No 2 (2021): November
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v3i2.751

Abstract

Air kelapa adalah senyawa alami kompleks yang sering digunakan sebagai pengatur tumbuh alami.   Penggunaan air kelapa sebagai bahan organik merupakan alternatif dari penggunaan bahan sintetis pada tanaman. Tujuan penelitian ini adalah untuk mengetahui pengaruh konsentrasi limbah dalam air kelapa dan air cucian beras serta lama perendaman terhadap perkecambahan biji kacang hijau. Parameter yang diamati adalah tinggi globul, jumlah radikula, dan panjang radikula. Berdasarkan hasil penelitian, air kelapa dan air cucian beras berpengaruh terhadap tinggi bulu dimana bulu tertinggi terdapat pada kombinasi (20 ml air kelapa + 80 ml air cucian beras dan perendaman 2 jam). Panjang radikula terpanjang ditemukan pada kombinasi (10 ml air kelapa + 90 ml air cucian beras dan perendaman 6 jam). Jumlah akar paling banyak terdapat pada kombinasi (50 ml air kelapa + 50 ml air cucian beras dan perendaman 6 jam).
Infeksi Jamur Dermatofita Pada Penderita Mikosis Kuku Winda Irawati Zebua; Kiki Nurtjahja; Sartini Sartini
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 3, No 1 (2021): Mei 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v3i1.539

Abstract

Dermatophytes are a group of fungi that cause nail abnormalities. This study aims to determine dermatophyte fungal infections in patients with nail mycoses. The method used in this study is descriptive and the sample was taken by purposive sampling with the criteria of damaged toenails, with characteristics: nails are not shiny, brownish to black from the base to the tip then the nails are examined directly with 10% KOH and cultured using media Sabouraud Dextrose Agar (SDA). The fungi isolated on slanted agar containing SDA and each fungal species were then identified using preparations for microscopic examination. The results showed that from 30 samples, 8 (eight) nail samples were positive for dermatophytes, namely: 5 (five) people infected with the fungus Tricophyton mentagrophytes species, 2 (two) people infected with the Trichophyton rubrum fungus, and 1 (one) person infected with Epidermophyton floccosum..
Hydroponic Planting Training to Maintain Food Security of the People of Bandar Setia Village, Percut Sei Tuan District, Deli Serdang Regency, North Sumatra Miswar Budi Mulya; Kiki Nurtjahja; Mimmy Sari Syah Putri
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 8 No. 1 (2023): ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/abdimastalenta.v8i1.10083

Abstract

The people of Bandar Setia Village experienced the impact of the implementation of PPKM due to regulations delimiting community activities. The limited agricultural land in the village also affects the fulfillment of food needs, especially vegetables. The purpose of this community service is to train the Forum Peduli Sesama Seniman Indonesia Cabang Tembung group representing the community of Bandar Setia in terms of hydroponic farming as an alternative to providing food stocks for the community in the village during the pandemic. The specific target of the training is the hydroponic model that can be made by the people of Bandar Setia village. The method used mixing counseling, training, hands-on practice, and work evaluation. Hydroponics manufacturing uses a mixing of NFT (nutrient film technique) and wick system methods. Vegetables harvested using hydroponics can be used for personal needs or sold according to the needs of the people of Bandar Setia village.
Preliminary Study on Potential Bacterial Species Introducing Antibacterial Agent from Sponge in Unggeh Island Central Tapanuli, Indonesia Latifah Ningrum Abdillah; Dwi Suryanto; Kiki Nurtjahja; Jendri Mamangkey
Jurnal Penelitian Pendidikan IPA Vol 10 No 7 (2024): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i7.8264

Abstract

Sponges have the potential to symbiont with bacteria that produce antibacterial compounds. This study was conducted to isolate and test the antibacterial activity of sponge symbiont bacteria. Bacterial isolates obtained were tested for antibacterial activity against Staphylococcus aureus, Bacillus subtilis and Escherichia coli using the Kirby-Bauer method. The identification results of both sponges have similarities with Clathria sp. and Hyrtios sp. symbiont bacteria obtained from both sponges as many as 16 isolates. Antibacterial activity testing had a weak inhibitory effect on isolates Sp1A, Sp2C, Sp2D and Sp2F, while Sp2J showed moderate inhibition against gram-negative and positive bacteria. The antibacterial activity of the whole supernatant of symbiont bacteria obtained the diameter of the inhibition zone with weak, medium and strong categories. Sp1A isolate supernatant was obtained, potentially able to fight Escherichia coli bacteria with a strong category as a representation of gram-negative bacteria and against gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus) classified into the medium category. In conclusion, 16 symbiont bacterial supernatants have antibacterial activity mostly active against gram-negative bacteria Escherichia coli and gram-positive bacteria Bacillus subtilis. There are 6 isolates (Sp1A, Sp1B, Sp1E, Sp1F, Sp2D, Sp2F) active as antibacterial against Staphylococcus aureus with weak and moderate categories.
Respon Fisiologis Pulutan (Urena lobata L.) terhadap Polusi Udara di Lingkungan Industri Winda, Winda; Kardhinata, Harso; Nurtjahja, Kiki; Fauziah, Ida
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 6, No 1 (2024): Mei
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v6i1.3960

Abstract

Air pollution, primarily originating from transportation and industrial activities, has become a major concern in the context of environmental health. This study aims to investigate the relationship between air pollution and the morphological characteristics of leaves as well as the stomatal density of the pulutan plant (Urena lobata L.) in polluted environments within the industrial area of Medan and unpolluted environments in Percut Sei Tuan, Medan, North Sumatra. The research method employed is ex-post facto with a laboratory approach to the polluted industrial area. Experimental results indicate that the stomatal density on pulutan leaves in the polluted area, specifically in the Industrial Zone of Medan, is 17.27/mm2, whereas in the unpolluted area, namely Percut Sei Tuan, it is 896.81/mm2. This difference provides insights into the negative impacts of air pollution on pulutan plants, highlighting the importance of maintaining air quality for ecosystem health and human well-being.
Potensi Bakteri Endofit dari Akar Kecombrang (Etlingera elatior (Jack) R. M. Smith) dalam Menghambat Pertumbuhan Staphylococcus aureus Lubis, Nurul Abdillah; Nurtjahja, Kiki; Rahmiati, Rahmiati; Susilo, Ferdinand
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 6, No 1 (2024): Mei
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v6i1.3959

Abstract

Endophytic bacteria are bacteria that live within plant tissues and are capable of producing secondary metabolites in the form of bioactive compounds similar to those produced by their host plants. This study aims to isolate and characterize endophytic bacteria from the roots of kecombrang (Etlingera elatior (Jack) R. M. Smith) and to test their ability to inhibit the growth of Staphylococcus aureus. The research was conducted using a quantitative descriptive method through several stages, including sample preparation, sterilization of equipment and media, isolation, characterization, and testing. The results identified three isolates of endophytic bacteria from the roots of kecombrang, namely AK1, AK2, and AK3, which were shown to be gram-positive cocci. Inhibition tests indicated that these endophytic bacteria exhibit antagonistic properties against Staphylococcus aureus. The isolate coded AK1 had an inhibition zone of 9.6 mm, AK2 had the lowest inhibition zone of 6.6 mm, and AK3 had the largest inhibition zone of 13.5 mm.
The Effect of Basil Leaves (Ocimum sanctum L.) as Natural Preservative for Mackerel (Rastrellinger sp.) Rahmiati, Rahmiati; Nurtjahja, Kiki; Nasution, MHD. Fauzi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i1.3895

Abstract

Basil is a plant that can be used as a traditional medicine. One part of the basil plant used as an alternative medicine is the leaves. Basil leaves contain active compounds, including flavonoids, saponins, tanins, and essensial oils. These active compounds is able to prevent the presence of pathogenic bacteria that can accelerate food spoilage, so that basil leaves can be used as an alternative natural preservative. The objective of the study was to determine the ability of basil leaves as a natural preservative for  mackerel. The research was carried out in vitro, by observing the physical, chemical, and microbiological characteristicts of mackerel which were treated 8 hours of soaking basil leaf extract. Variations in the concentration of basil leaves used were 0, 25, 50, 75 and 100%. The results showed that the physical quality of the 8-hour storage samples showed changes in the physical quality of the fish, as seen from the color, eyes, gills, mucus, aroma and texture. The microbioloical quality of fish was analyzed by the total plate number method. The total number of bacteria decreased as the concentration of basil leaf extract increased
Co-Authors Agnes Dame Sinta Uli Cut Fatimah Zuhra DAYAR ARBAIN DEDI PRIMA PUTRA Deny Supriharti Dwi Suryanto DWI SURYANTO Elimasni Erman Munir Erni Jumilawaty Eryna Elfasari Rangkuti Etti Sartina Siregar Ferdinand Susilo GINTA RIO Hesti Wahyuningsih Ida Fauziah Ida Fauziah, Ida Ira Wulan Dani Irene Banjarnahor Irene Banjarnahor, Irene Isnaini Nurwahyuni Jendri Mamangkey Julius Paolo Siregar Kaniwa Berliani Kardhinata, Harso Latifah Ningrum Abdillah Lisa Novita Arios Lubis, Nurul Abdillah Masitta Tanjung Mimmy Sari Syah Putri Miswar Budi Mulya Muhammad Yusuf Muhammad Yusuf Nasution, MHD. Fauzi Novi Malinda NUNUK PRIYANI Okky Setyawati Dharmaputra Rahmiati Rahmiati Rahmiati Rahmiati Riska Annisa Putri Riyanto Riyanto Rizal Syarief Rosliana Lubis Rosliana Lubis, Rosliana Sartini Sartini Sri Dwi Hastuti Srimaulinda Srimaulinda TATA BINTARA KELANA Ulfa Mashitah Winda Irawati Zebua Winda Winda Winiati Pudji Rahayu Yurnaliza, Yurnaliza