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All Journal Bio-Pedagogi : Jurnal Pembelajaran Biologi Microbiology Indonesia Biomedical Engineering Jurnal Florea AL ISHLAH Jurnal Pendidikan JPM (Jurnal Pemberdayaan Masyarakat) Jurnal SOLMA Edumaspul: Jurnal Pendidikan Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika Advances in Tropical Biodiversity and Environmental Sciences Bubungan Tinggi: Jurnal Pengabdian Masyarakat Indian Journal of Forensic Medicine & Toxicology Proceeding Biology Education Conference Berkala Penelitian Hayati Journal of Biodiversity and Biotechnology Jurnal Penelitian Pendidikan Indonesia (JPPI) Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Supiana Dian Nurtjahyani
Universitas PGRI Ronggolawe
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Journal : Biomedical Engineering

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Detection of Gen Chloramphenicol Acetyl Transferase (CAT ) Salmonella typhi Resistant Chloramphenicol by Polymerase Chain Reaction (PCR) Technique Supiana Dian Nurtjahyani; Retno Handajani
Biomedical Engineering Vol 3, No 1 (2017): Vol. 3 No. 1 Biomedical Engineering
Publisher : Biomedical Engineering

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Abstract

Objective: Salmonella typhi as a causative agent of typhoid abdominalis many militias, which was resistant to the drug, one of which is chloramphenicol. Detection of Salmonella typhi serologically generally done so if there is a problem in the diagnosis difficult to overcome so it needs a faster diagnosis techniques with accurate results. PCR can be used as an alternative solution to these problems. The purpose of this study was to detect the DNA of Salmonella typhi resistant to chloramphenicol by PCR.Material and methods: The method in this population experimental laboratory conducted in the laboratory biology and laboratory Unirow Tuban ITD Airlangga University Surabaya. Experiment done using PCR from blood samples. Using primer from CAT gene to detect this gene.Result: The results of this study with tank PCR can detect DNA of chloramphenicol resistant Salmonella typhi with a length of 293 bp.Conclusion: The conclusion PCR technique can detect DNA of Salmonella typhi resistant to chloramphenicol.
Detection of Hepatitis C Virus RNA in Blood Donors with Nested PCR Technique Supiana Dian Nurtjahyani; Mochammad Amin Amin; Retno Handajani Handajani
Biomedical Engineering Vol 2, No 2 (2016): Biomedical Engineering
Publisher : Biomedical Engineering

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Objective: This study was conducted to detect hepatitis C virus (HCV) RNA among blood donors in Tuban, East Java, Indonesia,  using nested Polymerase Chain Reaction (PCR) technique.Material and methods: Seven blood donors  with positives anti-HCV were obtained from 500 blood donors from Red Cross Blood Donor Center (Palang Merah Indonesia = PMI) Tuban, East Java, Indonesia, consisting of 375 men (75%) and 125 women (25%). Detection of anti-HCV antibody prevalence in the blood donors was done by using Enzyme-Linked Immunosorbent Assay (ELISA).  The detection of HCV RNA in blood donors  sera  with positives  anti-HCV were performed using nested-PCR technique with three set of primer pairs targeting the NS5B and 5’UTR regions of HCV.  Further amplification products were visualized on a 2% agarose gel containing ethidium bromide under  UV illumination.Result: Seven positives anti-HCV blood donors sera (1.4%) were obtained from 500 blood donors sera, in PMI Tuban, East Java, Indonesia. Out of seven blood donors sera with positives anti-HCV, 6 sera (85,7%)  were positives HCV RNA by using nested PCR technique. Five HCV RNAs were detected with two set of primers based on NS5B HCV region and 1 positive HCV RNA was detected with another set of primer based on 5’UTR HCV region.Conclusion: HCV RNAs were detected in 85,7% positives anti-HCV blood donors using nested PCR technique, in PMI Tuban, East Java. Further research is needed to determine the  genotype  and  subtype  of  HCV. 
Co-Authors Agusti Agusti Ahmad Yogi Pambudi Ahmad Zainal Arifin Ainur Rofieq Ali Mustafa Anik Winarni Arga Dias Pratama Dias Pratama Arifin, Ahmad Zaenal Avivi Nur Aina Christina Innocenti Tumiar Panggabean Dede Nuraida Devi Shintya Agustin Devi Shyntya Agustin Dwi Oktafitria Dwi Oktafitria Dwi Oktafitria Dwi Oktavitria Eko Purnomo Eko Purnomo Eko Purnomo eko purnomo Eko Purnomo Eko Purnomo Endah Mutiara Marhaeni Putri Fajrina Zulfa Darumiarsi Gea Elina Handajan, Retno Hanifa Aasaa Mufida Imas Cintamulya Indah Yana Indra Sugiarsi Kholisotul Hidayah Miftahus Surur Mochammad Amin Mohammad Amin Muhammad Nadjib Mujahid Nia Nurfitria Nurfitria Nova Maulidina Nova Maulidina Nugroho Budi Utomo Nurrohman Nurrohman Nurrohman, Endrik Nurul Hidayah Rafwan Year Perry Burhan Restiani Agusvita Retno Handajani Riris Suharti Lestari Roimil Latifa Rr. Eko Susetyarini Ruril Rudianto Spanton, Perdana Ixbal Sri Wulan Sri Wulan Sriwulan, Sriwulan Sukisno Sulistiono Tabitha Sri Hartati Wulandari Wahyu Nur Susanto Zuli Nofiyanti