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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Agrokompleks
Sari Nurulita
Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Veterinary

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Molecular Characterization of Begomovirus Infecting Yard Long Bean (Vigna unguiculata subsp. sesquipedalis L.) in Java, Indonesia Nurulita, Sari; Hidayat, Sri Hendrastuti; Mutaqin, Kikin Hamzah; Thomas, John
BIOTROPIA Vol. 22 No. 1 (2015): BIOTROPIA Vol. 22 No. 1 June 2015
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2015.22.1.401

Abstract

Begomovirus has been identified as one of the causal agents associated with yellow mosaic disease in yardlong bean (Vigna unguiculata subsp. sesquipedalis (L.)) in Java. Previous studies reported that Begomovirus has infected several Leguminosae species in South Asia, and several Begomoviruses have also been reported to infect important crops in Indonesia based on nucleotide sequence characterization. This study was conducted to identify and characterize Begomovirus isolated from yardlong bean samples in Java based on specific genomic features of the Common Region. Samples were collected from yardlong bean fields in Central Java (Tegal, Magelang, and Klaten), Yogyakarta (Sleman), and West Java (Bogor and Subang); viral detection was performed using I-ELISA, PCR, and sequencing; and molecular characterization was conducted using BioEdit v.7.0.5 and MEGA 6.06 software. Yellow mosaic disease was observed in nearly all surveyed fields, and infection of Potyvirus and Begomovirus was detected either as single or mixed infection. PCR results confirmed Begomovirus infection in samples from Tegal, Klaten, Magelang, Subang, and Bogor. Sequence analysis revealed that the virus infecting yardlong bean was Mungbean Yellow Mosaic India Virus (MYMIV) and shared close genetic similarity with isolates from Bangladesh, India, Pakistan, and Nepal. Further analysis revealed conserved regions surrounding the Common Region, including the “TATA box” sequence, hairpin-loop structure, repetitive sequences, and the conserved nonanucleotide TAATATTAC. This study provides the first report of MYMIV infection in Indonesia.
Co-Authors Botek, Muhammad Damaiyanti, Kartika Catur Gede Suastika Giyanto GUSNAWATY HS, GUSNAWATY Hidayat, Sri Hikamwaty, Aisyah I Dewa Made Putra Wiratama I G. R. M. TEMAJA John Thomas, John Kikin H Mutaqin Lestari, Yuliana Ayu Makyorukty, Dhayanti Mawarni, Sofi Miftahkuhrohmah, Miftahkuhrohmah MIFTAKHUROHMAH MIFTAKHUROHMAH, MIFTAKHUROHMAH MUHAMMAD TAUFIK Ni Nengah Putri Adnyani PURNAMA HIDAYAT RR. Ella Evrita Hestiandari Safinah Surya Hakim, Safinah Surya Sianipar, Theresia Aprilia SRI HENDRASTUTI HIDAYAT Suryo Wiyono Syarni, Elisa Taufik, Muhammad Taufik Tri Wira Yuwati, Tri Wira Yusuf, Dewi Nurhayati