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Journal : Current Biochemistry

Immobilization of Rhizopus oryzae Lipase on Zeolit, CaCO3, Silica Gel, and Cow Bone Maria Bintang; Tri Panji; Susi Saadah
Current Biochemistry Vol. 2 No. 2 (2015)
Publisher : IPB University

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Abstract

Food production in Indonesia is constrained by the high cost of lipase that is still imported from abroad. To overcome this problem, research of food production has been conducted using crude extract of lipase produced by indigenous species of fungi Rhizopus oryzae. The R. oryzae is edible indicating that it is safe to be used in the production of food products. Enzymes have an ability to catalyze specific chemical reactions with high efficiency and low energy cost. Enzyme immobilization is a recovery techique that has been studied in several years, using supporting materials as a medium to help enzyme dissolutions to the substrate. Several supporting materials such as zeolit, CaCO3, silica gel, and cow bone were selected by its ability to adsorb lipase. CaCO3 shows enzyme loading rate respectively 99.46%, giving more lipase to adsorb than zeolit (90.69%), cow bone (91.56%), and silica gel (59.63%). In this research, condition factors, such as optimum pH, optimum temperature, and storage ability of the matrix were investigated. Free lipase reacts optimally at pH 7 and temperature 30°C. Identical result showed for lipase in cow bone. Lipase in CaCO3 reacts optimally at pH 8 and temperature 35°C. Lipase in zeolit and silica gel reacts optimally at pH 8 and temperature 30°C. Half life time estimation was one week in storage condition temperature at 4°C and each optimum pH.
Biosorption Copper (Cu) and Mercury (Hg) by Omphalina sp. using Batch, Rotary, Biotray, and Pack Bed Flow Methods Desi Purwaningsih; I Made Artika; Tri Panji; Suharyanto Suharyanto
Current Biochemistry Vol. 3 No. 1 (2016)
Publisher : IPB University

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Abstract

Heavy metal waste treatment often uses dangerous chemicals. Omphalina sp is a nonpathogenic fungi that can be used to reduce the harmful effects of waste treatment. The use of fungal biomass has advantages such as low operating costs, efficient, and high metal binding capacity, minimal sludge, metals can be recovered, biosorbent can be regenerated, raw materials available easily, can use inactivated microorganism, and does not require additional nutrients. In the present study optimization of the biomass utilization for waste water treatment was conducted by comparing batch, rotary, packbed flow, and biotray methods. Results showed that Omphalina sp can reduce mercury level up to 91.38% with rotary, 83.98% with biotray, 87.14% with pack bed flow, and 31.94% with batch methods respectively from initial Hg concentration of 3 ppm. Similarly, Omphalina sp can reduce copper level up to 23.58% with rotary, 22.66% with biotray, 10.53% with pack bed flow, 10.17% with batch methods respectively from initial Cu concentration of 100 ppm. Optimum absorption Hg and Cu occurs in the first one hour.
Deodorization of Latex Waste and Decolorization of Textile-Coloring Agent by Omphalina sp. using Batch and Continuous Methods Erna Puspasari; I Made Artika; Tri Panji
Current Biochemistry Vol. 3 No. 1 (2016)
Publisher : IPB University

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Abstract

Generally industrial waste water is a pollutant to environment as it produces strong odor and color. Omphalina sp is one of white rot fungi that can be used as an odor and color effluent reducer. Omphalina sp has laccase enzyme that plays role in deodorization and decolorization. The aim of this research was to determine the best method in deodorization and decolorization among batch and continous (pack bed flow, biotray, and rotary contactor) methods. The results of deodorization and decolorization showed that the rotary contactor method was better than the batch, pack bed flow and biotray methods. At dye concentration of 50 ppm, after 24 hours treatment, the absorbance value for batch, pack bed flow, biotray, and rotary contactor methods was 0.520, 0.423, 0.425, and 0.357, respectively. At dye concentration of 150 ppm, after 24 hour treatment, the absorbance value for batch, pack bed flow, biotray, and rotary contactor methods was 0.709, 0.629, 0.658, and 0.592, respectively. At dye concentration of 50 ppm, percentage of dye absorption after 24 hour treatment for batch, pack bed flow, biotray, and rotary contactor methods was 20.550%, 35.447%, 35.141% and 45.531% respectively. At dye concentration of 150 ppm, percentage of dye absorption after 24 hour treatment for batch, pack bed flow, biotray, and rotary contactor methods was 7.320%, 17.843%, 13.987%, and 22.614%. The qmaks value at dye concentration of 50 ppm after 24 hour treatment for batch, pack bed flow, biotray, and rotary contactor methods was 0.020, 0.076, 0.083, and 0.124 respectively. qmaks value at dye concentration of 150 ppm after 24 hour treatmeny for batch, pack bed flow, biotray, and rotary contactor methods was 0.009, 0.077, 0.046, and 0.100 respectively. Odor scale for batch, pack bed flow, biotray, and rotary contactor methods decreased from 5 to 2.9, 1.4, 1.6 and 1.1 respectively.