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Journal : Jurnal Biologi Universitas Andalas

Isolasi Dan Karakterisasi Bakteri Termo-Proteolitik Sumber Air Panas Sungai Medang, Sungai Penuh, Jambi Dina Wahyuna; Anthoni Agustien; - Periadnadi
Jurnal Biologi Universitas Andalas Vol 1, No 2 (2012)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.1.2.%p.2012

Abstract

A study on isolation and characterization of thermoproteolytic bacteria from hot springs at Sungai Medang, Sungai Penuh, Jambi was conducted from March to June 2012 using a descriptive method. Seventy thermophylic bacteria were isolated from the hot springs. A Total of 39 thermoproteolytic bacteria 39 were identified. The highest enzymatic activity (13.59 U/ml) was shown by isolate MI2.3 from a location at which the temperature was 600C. The isolate MI2.3 was characterized as an aerobic thermoalkaliphylic (optimum temperature of 500C, optimum pH of 9.0) bacterium with the ability to produce amylase but neither lipase nor cellulase.Keywords : alkaliphylic, bacterium, enzymatic activity, proteolytic, thermophylic.
Pengaruh Pengaturan Keasaman Limbah Industri Teh Terhadap Pertumbuhan Miselium Jamur Tiram Cokelat (Pleurotus cystidiosus O.K. Miller) Dalli Yullio Saputra; - Nurmiati; - Periadnadi
Jurnal Biologi Universitas Andalas Vol 2, No 4 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.4.%p.2013

Abstract

The study about the effect of acidity adjusting of industrial waste of tea production on the mycelium growth of brown oyster mushroom (Pleurotus cystidiosus O.K. Miller) was conducted from January to April 2013 in the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Padang. The aim of this study is to determine the effect of setting the acidity of tea production industrial waste and the mycelium growth of brown oyster mushroom. This research was designed by completely randomized design (CRD) with five treatments, which were pH 4, pH 5, pH 6, pH 7 and pH 8 in 5 replication. The results showed that the treatment of pH 8 and pH 7 on industrial waste of tea before weathering was optimum for the growth of mycellium (18 – 19,2 days) . Keywords: Acidity, waste of tea, Pleurotus cystidiosus, mycelium.
Potensi dan Karakterisasi Bakteri Alami Pencernaan Ayam Broiler Pedaging (Gallus gallus domesticus L.) Sebagai Kandidat Probiotik Pakan Ayam Broiler Adelia Febriyossa; - Nurmiati; - Periadnadi
Jurnal Biologi Universitas Andalas Vol 2, No 3 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.3.%p.2013

Abstract

A study about potential and characterization of native bacteria of intestinebroiler (Gallus gallus domesticus L.) as probiotic candidate of a broiler poultry product were conducted from December 2012 to March 2013 at Microbiology Laboratory, Department of Biology, Andalas University, Padang. This study aimed to obtainbacterial isolates and to describe morphological characters of each isolate of native bacteria as probiotic candidate for thebroiler.This study used an experimental method and analyzed descriptively. The results showed that rough material of intestine consisted of fermentative bacteria (57x107cfu/g), amylolitic (118x107cfu/g), cellulolytic (63x107cfu/g) and proteolytic bacteria (52x107cfu/g). Two isolates were potensial probioticcandidates; amylolytic bacteria and cellulolytic bacteria. The characteristics both probiotic candidates were gram negative, non-sporulating, positive catalase and motile.Keywords: Gallus gallus domesticus, intestine bacteria, characterization
Karakterisasi Mikroflora Alami Saluran Pencernaan Sapi Potong Sebagai Kandidat Probiotik Pakan Sapi Potong Uswatul Hasana; Nurmiati Nurmiati; Periadnadi Periadnadi
Jurnal Biologi Universitas Andalas Vol 2, No 4 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.4.%p.2013

Abstract

The research about the "Characterization of Natural Microflora From Cow Digestic Tract As Candidate For Cattle Feed Probiotics"  has been conducted from April to July 2013 in the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Andalas, Padang. This study aimed to obtain isolates  and the character of each isolate as a candidate to cattle feed probiotics. This study used an experimental method and analyzed descriptively. The result showed that the bacteria from cow digestive tract were Cellulolytic (110x107cfu/g), Fermentative (85x107cfu/g),  Amylolytic (31x107cfu/g) and Proteolytic (28x107cfu/g) bacteria. Two of  the probiotic candidate were bacill and coccus, both of them were Gram-negative and catalase positive.
Penapisan Bakteri Termo-Amilolitik Dari Sumber Air Panas Sungai Medang, Kerinci, Jambi Devi Syafriyani; Anthoni Agustien; - Periadnadi
Jurnal Biologi Universitas Andalas Vol 2, No 2 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.2.%p.2013

Abstract

This study aimed to observe the ability of isolated bacteria from Sungai Medang hot spring in producing amylase and to determine the activity and characteristic of isolated amylolytic bacteria which gave the highest amylolytic index. The water samples were collectied by using purposive sampling based on different level of temperatures. This study has successfully collected 48 bacterial isolated from the hot spring. To find the highest amylolytic activity, we tested ten isolated bacteria which have high amylolytic indices. We found that MV2.7 isolated from 740C of hot spring gave the highest amylolytic activity. The isolated bacteria was positive proteolytic, cellulolytic and negative lipolytic. The isolated bacteria showed higher and more spesific activity on rice starch rather than corn and sago starchKeywords: Screening, thermophilic, amylolytic, activity
Inventarisasi Jamur Tingkat Tinggi (Basidiomycetes) di Gunung Singgalang Sumatera Barat Indra Anggriawan; - Periadnadi; - Nurmiati
Jurnal Biologi Universitas Andalas Vol 3, No 2 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.2.%p.2014

Abstract

The macrofungi of Basidiomycetes was collected in Singgalang mountain and then identified in Microbiology Laboratory, Department of Biology, Faculty of Sciences, Andalas University. The aim of this study was to identify the macrofungi from Basidiomycetes. This study used direct survey method to collect macrofungi from the field. This study identified 22 genera which belong to 10 family and 6 orders. Most of the fungi were belong to subclass of Hymenomycetidae.Keywords: basidiomycetes, inventories, macrofungi.
Uji Antimikroba Ekstrak Segar Jahe-Jahean (Zingiberaceae) Terhadap Staphylococcus aureus, Escherichia coli dan Candida albicans Kartika Indah Permata Sari; - Periadnadi; Nasril Nasir
Jurnal Biologi Universitas Andalas Vol 2, No 1 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.1.%p.2013

Abstract

Seven species of Zingiberaceae were investigated for their antimicrobial activities against Staphylococcus aureus, Escherichia coli and Candida albicans. Extract of grinded rhizome was centrifuged at 10.000 rpm for five minutes. This study used completely randommized design in nested pattern with three replications. The first factor was the tested microbe and the second was the fresh extract of the ginger rhizome. The result showed that fresh extract of red ginger (Zingiber officinale var. Rubrum) gave the highest inhibition to the growth of S. aureus (15.83 mm) and E. coli (15.33 mm) while elephant ginger (Z. officinale var. Roscoe) gave the highest inhibition growth of C. albicans (10.7 mm).Key words: antimicrobial, ginger, microbial pathogen
Pengaruh Penambahan Ekstrak Daun dan Bubuk Teh, Kopi, dan Coklat Terhadap Fermentasi Nata De Coco Merry Tri Indah Permata Sari; - Periadnadi; - Nurmiati
Jurnal Biologi Universitas Andalas Vol 3, No 3 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.3.%p.2014

Abstract

This study aimed of to find out the effect of leaf extract and seed powder containing alkaloid which occured on the production process of nata de coco. An experiment was conducted using Complete Randomized Design (CRD) with factorial pattern. The result showed that the brewed extract of tea, coffee, chocolate affected the weight product of nata de coco. The brewed extract of green tea showed the best result on weight of the nata (24.66 g) which has a significant difference from the treatments of black tea, coffee powder, cocoa powder, coffee leaf, and chocolate leaf. The best dose of brewed extract in obtaining nata was 2 g/100 ml.Keywords : alkaloid, nata de coco, effect.
Isolasi Bakteri Pendegradasi Hidrokarbon di Tanah Tercemar Lokasi Perbengkelan Otomotif Hezi Yolantika; Periadnadi Periadnadi; Nurmiati Nurmiati
Jurnal Biologi Universitas Andalas Vol 4, No 3 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.3.%p.2015

Abstract

The research related to Isolation of the Hydrocarbon Degrading Bacteria in the Contaminated Soil of the Automotive Workshop was conducted from May to October 2014 at Laboratory of Microbiology, Biology Department, Faculty of Mathematics and Natural Sciences, Andalas University. The aimed of this study was to find  the bacteria able to degradate the hydrocarbon from the lubricating oil contaminated soil and to descripe characterization of isolates. This study used a survey method in isolation and characterization of hydrocarbon degrading bacteria.  The result obtained that the bacteria able to degradate the hydrocarbon from the lubricating oil contaminated soil about 39.02% (1.6x105 cfu/g). Five isolates were found (BTTO1, BTTO2, BTTO3, BTTO4 and BTTO5) has a rod shaped, Gram-negative, motile and positive catalase.