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All Journal HAYATI Journal of Biosciences AL-HAYAT: Journal of Biology and Applied Biology
Dyah Perwitasari
Fakultas Farmasi Universitas Ahmad Dahlan Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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Variations of Exon 2-3 of the Branched Chain Keto Acid Dehydrogenase E1 Subunit Alpha (BCKDHA) Gene and Its Flanking Intronic Region in Madura Cattle Febriana, Asri; Farajallah, Achmad; Wahidah, Baiq Farhatul; Perwitasari, Dyah
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 2 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i2.13436

Abstract

Indonesia is one of a country with high biodiversity and genetic resources. One of them is Madura cattle that derived from crossing between exotic cattle, namely zebu cattle with local Indonesian cattle, namely Bali cattle. Branched-chain alpha-keto acid dehydrogenase is an enzyme complex that metabolizes branched chain amino acids, namely valine, leucine and isoleucine. This study aimed to analyze exons 2-3 variation of the BCKDHA gene and its flanking region in Madura cattle. The sequences of it were obtained using DNA sequencing techniques. A total of seven variations were found, one variation located in the flanking region adjacent to the 5' end of exon 2 of the BCKDHA gene. The A392G variation found in exon 3 does not cause any amino acid changes. There is no variation found in exon 2, it indicates that the exon regions of BCKDHA gene are more conserved than introns in the Madura cattle.
Variations of Exon 2-3 of the Branched Chain Keto Acid Dehydrogenase E1 Subunit Alpha (BCKDHA) Gene and Its Flanking Intronic Region in Madura Cattle Febriana, Asri; Farajallah, Achmad; Wahidah, Baiq Farhatul; Perwitasari, Dyah
Al-Hayat: Journal of Biology and Applied Biology Vol. 5 No. 2 (2022)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v5i2.13436

Abstract

Indonesia is one of a country with high biodiversity and genetic resources. One of them is Madura cattle that derived from crossing between exotic cattle, namely zebu cattle with local Indonesian cattle, namely Bali cattle. Branched-chain alpha-keto acid dehydrogenase is an enzyme complex that metabolizes branched chain amino acids, namely valine, leucine and isoleucine. This study aimed to analyze exons 2-3 variation of the BCKDHA gene and its flanking region in Madura cattle. The sequences of it were obtained using DNA sequencing techniques. A total of seven variations were found, one variation located in the flanking region adjacent to the 5' end of exon 2 of the BCKDHA gene. The A392G variation found in exon 3 does not cause any amino acid changes. There is no variation found in exon 2, it indicates that the exon regions of BCKDHA gene are more conserved than introns in the Madura cattle.
New Distribution Record of Tetrigona apicalis (Smith, 1857)  (Hymenoptera: Apidae: Meliponini) in Ujung Kulon National Park, Indonesia Miharja, Jajang; Atmowidi, Tri; Priawandiputra, Windra; Perwitasari, Dyah; Kahono, Sih
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.1-7

Abstract

Ujung Kulon National Park (UKNP) is located in the western part of Java Island, Indonesia. This national park was designated a Natural World Heritage Site by UNESCO in 1992. The stingless bee species, Tetrigona apicalis (Apidae: Meliponini), has not previously been documented in specific locations on Java Island. In this study, we found a new distribution record of T. apicalis in UKNP. Here, we described of its nest-entrance characteristics, morphological features, and morphometry. The colony of bee species was found in the forest of Mount Honje, UKNP, Indonesia, at an altitude of 883 m asl. The nest is located in a Tiliaceae tree cavity at a height of 420 cm from the ground surface. The shape of the nest entrance of the bee species was irregular, with a longitudinal diameter of 3.2 cm and a vertical diameter of 5.7 cm, and it had a light brown color. Locally, this bee is known as teuwel meunyan, referring to a traditional incense burner due to the aromatic scent associated with its use in religious rituals. The findings contribute to the current knowledge of the distribution of T. apicalis on Java Island, particularly within UKNP, and provide data for future conservation and sustainable utilization efforts.
Co-Authors ACHMAD FARAJALLAH Asri Febriana, Asri Baiq Farhatul Wahidah Miharja, Jajang Sih Kahono Tri Atmowidi WINDRA PRIAWANDIPUTRA, WINDRA