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All Journal JURNAL BIOMEDIK EUGENIA JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI Jurnal Agroekoteknologi Terapan Journal of Agriculture (JoA) Bulletin of Social Informatics Theory and Application COCOS
Jantje Pongoh
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Environmental Science Health Professions Social Sciences

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The Effect Of Pgr Kinetin And Coconut Water On The Growth And Development Of The Orchid Dendrobium mirbelianum In Vitro Tungga, AgnesTheresia; Tilaar, Wenny; Tulung, Stella Maria Theresia; Pongoh, Jantje; Mamarimbing, Rinny; Sumayku, Bertje R. A.
Jurnal Agroekoteknologi Terapan Vol. 4 No. 2 (2023): EDISI JULI-DESEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v4i2.51921

Abstract

This research aims to determine the effect of the concentration of ZPT kinetin and coconut water on the growth and development of orchid shoots. This research was carried out at the Biotechnology Laboratory, Faculty of Agriculture, Sam Ratulangi University Manado from May to June 2023. This research used a factorial experiment in a Completely Randomized Design (CRD) with 5 replications consisting of two factors. The first factor is Kinetin concentration; 1 mg/l (K1), 2 mg/l (K2) and 3 mg/l (K3) and the second factor is Coconut Water Composition; Coconut water 10% (A1), Coconut water 20% (A2), Coconut water 30% (A3) and Coconut water 40% (A4). Data were analyzed using ANOVA and if there was a significant effect, it was continued with the 5% BNT test. The variables observed were: shoot height, number of leaves, number of shoots, and wet weight. The results showed that there was a significant effect on the number of shoots on the single factor Kinetin while the others were not significantly different between the combination of Kinetin and coconut water on the growth and development of orchid shoots, namely on the variable height of shoots, number of shoots, number of leaves and percentage of wet weight of Dendrobium orchid shoots mirbelianum. Keywords: Orchid, Dendrobium mirbelianum, Kinetin, Coconut water, In vitro Abstrak Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi penggunaan ZPT kinetin dan air kelapa terhadap pertumbuhan dan perkembangan tunas anggrek. Penelitian ini dilaksanakan di Laboratorium Bioteknologi, Fakultas Pertanian, Universitas Sam Ratulangi Manado dari bulan Mei sampai Juni 2023. Penelitian ini menggunakan percobaan faktorial dalam Rancangan Acak Lengkap (RAL) dengan 5 ulangan yang terdiri dari dua faktor. Faktor pertama yaitu konsentrasi Kinetin; 1 mg/l (K1), 2 mg/l (K2) dan 3 mg/l (K3) dan faktor kedua Komposisi Air Kelapa; Air kelapa 10% (A1), Air kelapa 20% (A2), Air kelapa 30% (A3) dan Air kelapa 40% (A4). Data dianalisis dengan ANOVA dan jika berpengaruh nyata maka dilanjutkan dengan uji BNT 5 %. Variabel yang diamati adalah: tinggi tunas, jumlah daun, jumlah tunas, dan berat basah. Hasil penelitian menunjukan bahwa terdapat pengaruh nyata terhadap jumlah tunas pada faktor tunggal Kinetin sedangkan yang lain tidak berbeda nyata antara kombinasi kinetin dan air kelapa terhadap pertumbuhan dan perkembangan tunas anggrek yaitu pada variabel tinggi tunas, jumlah tunas, jumlah daun dan persentase berat basah tunas anggrek Dendrobium mirbelianum. Kata Kunci: Anggrek, Dendrobium mirbelianum, Kinetin, Air Kelapa, In vitro
Proximate Analysis Of Collagen Cockatoa Fish Scales (Scarus sp.) Herson, Nur Afiah; Sumual, Maria Fransisca; Rumengan, Inneke F. M; Pongoh, Jantje; Mandey, Lucia C.
Jurnal Agroekoteknologi Terapan Vol. 4 No. 2 (2023): EDISI JULI-DESEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v4i2.52484

Abstract

Collagen is the main component of the dermis layer of skin (bottom of the epidermis) which is made by fibroblast cells. Collagen sourced from marine biological materials in the form of properly extracted fish scales can be an alternative source to collagen from cows and pigs. This research aims to analyze the composition of the collagen material (proximate) of Cockatua fish (Scarus sp) scales. This research was carried out in March 2023 – August 2023 at the regional technical implementation unit, Laboratory for Development and Quality Testing of Fishery Products, Gorontalo Province. Proximate analysis of parrot fish scale collagen was carried out on four parameters, namely water content, ash content, fat content and protein content. The proximate analysis method refers to the SNI 2354.2:2015 (moisture content), SNI 01-2354.1-2006 (dust content), SNI 01-2354.4-2006 (protein content), SNI 2354-3:2017 (fat content). The water content of parrotfish scale collagen is 4.35%, ash content is 0.76%, protein content is 82.17% and fat content is 1.18%. From the results of the proximate analysis it is known that all observation parameters have met BSN 8076:2014 standards. Keywords : Collagen, parrot fish scale, proximate Abstrak Kolagen merupakan komponen utama lapisan kulit dermis (bagian bawah epidermis) yang dibuat oleh sel fibroblast. Kolagen yang bersumber dari bahan hayati laut berupa sisik ikan yang terekstraksi dengan tepat dapat menjadi sumber alternatif selain kolagen dari sapi dan babi. Penelitian ini bertujuan untuk menganalisis komposis bahan (proksimat) kolagen sisik ikan kakatua (Scarus sp). Penelitian ini dilaksanakan pada bulan Maret 2023 – Agustus 2023 di unit pelaksana teknis daerah Laboratorium Pembinaan dan Pengujian Mutu Hasil Perikanan Provinsi Gorontalo. Analisis proksimat kolagen sisik ikan kakatua dilakukan pada empat parameter yaitu kadar air, kadar abu, kadar lemak dan kadar protein. Metode analisis proksimat mengacu pada metode SNI 2354.2:2015 (kadar air), SNI 01-2354.1-2006 (kadar debu), SNI 01-2354.4-2006 (kadar protein, SNI 2354-3:2017 (kadar lemak). Hasil penelitian menunjukkan kadar air dari kolagen sisik ikan kakatua yaitu 4.35 %, kadar abu 0.76 %, kadar protein 82.17 % dan kadar lemak 1.18 %. Dari hasil analisis proksimat diketahui seluruh parameter pengamatan telah memenuhi standar BSN 8076:2014. Kata kunci : kolagen, sisik ikan kakatua, proksimat.
COMPOUND CONTENT OF LOCAL CURCUMIN (CURCUMA XANTHORRHIZA) IN NORTH SULAWESI, INDONESIA Demmassabu, Langimanapa Sofia; Paat, Frangky Jessy; Turang, Deflly Ansye Shilfana; Tumbelaka, Selvie; Mamuaja, Christine F; Wantasen, Sofia; Toding, Marjam M.; Pongoh, Jantje; Paulus, Jeanne Martje
Journal of Agriculture Vol. 2 No. 02 (2023): Research Articles, July 2023
Publisher : ITScience (Information Technology and Science)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47709/joa.v2i02.2751

Abstract

This Zingiberaceae plant is widely used as medicine, including temulawak. Because curcuma (C.xanthorrhiza) is a medicinal plant that has many benefits and includes plants needed in large quantities compared to other medicinal plants. Traditionally rhizomes Temulawak is used to treat stomach ailments, liver disorders, constipation, diarrhea, dysentery, fever, hemorrhoids, hypotriglyceridaemic, and anti-inflammatory. Study about plant Curcuma local ignite still not enough researched so that not yet get information compounds which contained in the local curcuma of North Sulawesi for the development for biopharmaceutical development, mapping in the distribution of plants, ecology, plant conservation and protection to maintain local wisdom. Histochemical Test is a method to determine the content of chemical compounds in a plant tissue qualitative. Testing can be done by adding a special reagent or solution to the incision organ plant and will give color which Specific. Activity study this will test curcumin compound group on local ginger from North Sulawesi by histochemical method. research that done is study non experiment with design descriptive qualitative. The results showed that North Sulawesi local white temulawak powder contained curcumin in a sample with a sample weight of 0.10 g at a sample spotting volume of 20 µl with a sample spotting volume of 2040 nanograms, curcumin levels were <0.10 nanograms/mg. Curcumin biosynthesis is influenced by site conditions, agro-climate, genotype, and plant cultivation.
Co-Authors A.G. Tulungen Arthur G. Pinaria Berce.R.A Sumayku Bobby Polii Dedie Tooy Demmassabu, Langimanapa Sofia Edy F. Lengkong Erwin G. Kristanto Frangky J. Paat Giavirna J. Enoch Heldering Tampake Herson, Nur Afiah Inneke F. M Rumengan Islam, Noorul J. M. Paulus Jeanne Martje Paulus, Jeanne Martje Lavenia Mamahit Lucia Cecilia Mandey Luther Latumakulita Mamuaja, Christine F Mandagi, Franklin Marselina Nebangka Paat, Frangky Jessy Paula C.H. Supit Pioh, Diane Rinny Mamarimbing Sandra Pakasi Selvie Tumbelaka Semuel D. Runtunuwu Semuel David Runtunuwu Sofia Wantasen Stella Maria Theresia Tulung Sumayku, Bertje R. A. Sumual, Maria Fransisca Tenda, Edwin TODING, MARJAM M. Tuladhani, Endah Tulung, Stella Maria Theresia Tungga, AgnesTheresia Turang, Deflly Ansye Shilfana Varyanti Magdalena Turang Vilma Victa Rikumahu Wawointana, Adeleida Ch. Wenny Tilaar Yefta Pamandungan Yefta Pamandungan