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Aktivitas Antioksidan dan Total Polisakarida Ekstrak Rumput Laut Merah, Hijau dan Coklat dari Pantai Jangkar Situbondo Didin Erma Indahyani; Depi Praharani; Izzata Barid; Ari Tri Wanodyo Handayani
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 16 No 2 (2019)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v16i2.23094

Abstract

The antioxidant activity and polysaccharide composition of seaweed is influenced by species (red, green and brown seaweed), geographical conditions, extraction methods, solvents, temperature and the length of time of extraction. The purpose of this study was to known the total polysaccharide and antioxidant activity in red, green and brown seaweed extracts from Jangkar Situbondo beach. Method. The study was conducted on red, green and brown seaweed extracted with ethanol and water to determine total polysaccharides. Antioxidant analysis uses the method 2,2-diphenyl1-picrylhydrazyl (DPPH) radical. Total polysaccharide and antioxidant activity were observed statistically using two-way ANOVA. The results of this study show that the polysaccharide content in seaweed water extract was significantly higher than ethanol extract, especially in brown seaweed. Seaweed water extracts showed higher antioxidant activity than ethanol extracts. The antioxidant activity of brown seaweed was significantly higher than that of green and red seaweed. Conclusion. Seaweeds have high antioxidant activity in water extracts. Brown seaweed water extract contains high polysaccharides than green and red seaweed. Antioxidant activity in brown seaweed is also higher than in green and red seaweed.
Pengaruh kebersihan mulut dengan kesehatan gingiva pada pemakai alat orthodontik cekat Effect of oral hygiene and gingival health in fixed orthodontics appliances Iga Nadya Putri; Depi Praharani; Peni Pujiastuti; Dwi Prijatmoko; Elyda Akhya Afida Misrohmasari
Padjadjaran Journal of Dental Researchers and Students Vol 6, No 3 (2022): Oktober 2022
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v6i3.40327

Abstract

ABSTRAK Pendahuluan: Alat orthodontik cekat adalah salah satu alat yang digunakan untuk perawatan maloklusi. Bagian-bagian alat tersebut menempel pada gigi yang menyulitkan dalam pembersihan gigi, sehingga akan memudahkan akumulasi plak dan dapat menyebabkan gingivitis. Tujuan penelitian ini adalah menganalisis  hubungan antara kebersihan mulut dengan kesehatan gingiva pada pemakai alat orthodontik cekat. Metode: Jenis penelitian observasional dengan rancangan penelitian cross-sectional study. Subjek penelitian mahasiswa Fakultas Kedokteran Gigi Universitas Jember yang  memakai alat orthodontik cekat yang diambil dengan metode purposive sampling. Data penelitian diambil dengan kuesioner, pengukuran tingkat kebersihan mulut dengan OHI-S (Green dan Vermilion), dan kesehatan gingiva dengan Indeks Gingiva (Loe dan Silness). Hasil: Sebanyak 17% subjek memiliki tingkat kebersihan rongga mulut baik, 57% sedang, 26% buruk, dan rerata tingkat kebersihan mulutnya adalah sedang. Sebanyak 89% subjek mengalami gingivitis  ringan, 11% sedang, tidak ada yang mengalami gingivitis berat, dan rerata status kesehatan gingivanya adalah gingivitis ringan. Uji korelasi Pearson menunjukkan nilai korelasi (r=0,665) yang artinya terdapat hubungan yang kuat antara kebersihan mulut dengan kesehatan gingiva.  Uji regresi linier didapatkan nilai r2= 0,442 yang berarti kebersihan mulut berhubungan kuat dengan kesehatan gingiva sebesar 44,2%. Simpulan: Semakin buruk tingkat kebersihan mulut pada pemakai alat orthodontik cekat maka status kesehatan gingivanya juga semakin buruk. Kata kunci: kebersihan mulut; kesehatan gingiva; alat orthodontik cekat; OHI-S; indeks gingiva.ABSTRACTIntroductions: A fixed orthodontic device is one of the tools used for the treatment of malocclusion. The parts of the tool attach to the teeth which make it difficult to clean the teeth, so that it will facilitate the accumulation of plaque and can cause gingival inflammation.  To determine whether there is a relationship between oral hygiene and gingival health in students of the Faculty of Dentistry, University of Jember who use fixed orthodontics. Methods: Observational study with a cross–sectional study design. The research subjects were students of the Faculty of Dentistry, University of Jember who used fixed orthodontic appliances which were taken using purposive sampling method. The research data was collected by using a questionnaire, measuring the level of oral hygiene with OHI-S (Green and Vermilion), and gingival health using the Gingival Index (Loe and Silness). Results: 17 % of subjects had good oral hygiene levels, 57 % were moderate, 26 % were poor, and the average level of oral hygiene was moderate. As many as 89 % of subjects had mild gingival inflammation, 11 % were moderate, no one had severe gingival inflammation, and the average gingival health status was mild gingival inflammation. The Pearson correlation test showed a correlation value (r = 0.665) which means that there is a strong relationship between oral hygiene and gingival health. In the linear regression test the value of r2 = 0.442 means that the effect of oral hygiene on gingival health is 44.2 %. Conclusion: In users of  fixed orthodontics, the level of oral hygiene is better, the health status of the gingiva is getting better.Keywords: fixed orthodontics; gingival disease; gingival index; malocclusion; OHI–S.
Management of Localized Gingival Overgrowth in Patient under Fixed Orthodontic Treatment: A Case Report Neira N. Sakinah; Yuliana M. D. Arina; Depi Praharani; Peni Pujiastuti; Desi S. Sari; Melok A. Wahyukundari
e-GiGi Vol. 10 No. 2 (2022): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v10i2.40683

Abstract

Abstract: Recently, the need for orthodontic treatment has increased along with the increasing need and public awareness of the importance of dental and oral health. During treatment, problems sometimes occur with periodontal tissue. Orthodontic devices that bond to the tooth surface can cause difficulties in controlling plaque, especially in marginal areas which can cause inflammation and gingival overgrowth. Too high tensile strength and weak tissue capacity are also the factors that can cause gingival overgrowth. This case report described the management of gingival growth in a patient under fixed orthodontic treatment. We reported an 18-year-old female patient with a good systemic condition that had gingival overgrowth localized on the anterior part of mandibula. The surgical intervention was performed by using conventional surgical methods. Its goal was to remove the gingival pocket, therefore, the plaque could be easily controlled. The use of a scalpel was considered very effective for eliminating excessive gingiva. Two weeks after surgical treatment, the gum resulted in good repair. The continuity of orthodontic treatment could be achieved properly after 3-month surgical treatment. Instructions and motivation to maintain oral hygiene were important to avoid recurrence. In addition, multidisciplinary dentistry between orthodontist and periodontist was needed to achieve a better result. Keywords: gingival overgrowth; gingivectomy; orthodontic appliance
The potential of toothpaste containing Robusta coffee bean extract in reducing gingival inflammation and dental plaque formation Peni Pujiastuti; Neira Najatus Sakinah; Yuliana Mahdiyah Da’at Arina; Melok Aris Wahyukundari; Depi Praharani; Desi Sandra Sari
Dental Journal Vol. 56 No. 2 (2023): June 2023
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i2.p109-114

Abstract

Background: The prevention of gingivitis using chemicals containing antibiotics and chlorhexidine can disrupt the balance of the oral microbiota and have side effects in long-term use. A recent development in the prevention of gingivitis is the use of natural ingredients. Coffee is a natural ingredient that compounds several antibacterial and anti-inflammation properties. Purpose: The study aimed to determine the potential of toothpaste containing Robusta coffee bean extract in reducing gingival inflammation and inhibiting the formation of dental plaque. Methods: Twenty male Rattus norvegicus were divided into four groups, namely the control group and treatment groups (TG) TG25%, TG50%, and TG75%. All groups were fitted with ligature wire on the first left molar to accumulate dental plaque. After the fourth day, the ligature wire was removed, and the TG25%, TG50%, and TG75% groups were brushed once a day using toothpaste containing various concentrations of Robusta coffee extract, while the control group was brushed without using toothpaste. Plaque index, gingival index, and interleukin-1 (IL-1) expression were observed on the fifth day. The data was statistically tested using a one-way analysis of variance and post hoc least significant difference. Results: The statistical test showed that the TG75% group had the lowest value of plaque, gingival index, and IL-1 expression, while the control group had the highest (p < 0.05). Conclusion: Robusta coffee bean extract toothpaste has the potential to reduce gingival inflammation and dental plaque formation in a rat with gingivitis. The most effective concentration of Robusta coffee bean extract toothpaste in reducing gingival inflammation and dental plaque formation was 75%.
Aktivitas Antibakteri Bahan Cetak Berbasis Ekstrak Natrium Alginat dari Alga Merah (Kappaphycus alverezii) terhadap Lactobacillus acidophilus Putu Sadaka Putri Rahayu; Depi Praharani; Niken Probosari; Didin Erma Indahyani; Izzata Barid
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 20 No 1 (2023)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v20i1.38592

Abstract

Alginate impression material is a material that is often used for impression tissue in the oral cavity, and can be one of the agents of infection transmission in dental practice. Microorganisms can adhere to the mold such as Lactobacillus acidophilus bacteria. L. acidophilus were isolated from active caries and were considered as secondary invaders in the dental caries invasion process. The development of impression materials that have antibacterial capabilities needs to be done, one of which is by using materials derived from red algae (Kappaphycus alverezii). This plant contains a lot of alginate which is the main components of the cell wall, consisting of calcium, magnesium, sodium and potassium alginate salts. Synthesizing sodium alginate from red algae and analyzing the antibacterial activity of sodium alginate-based impression materials from red algae against L. acidophilus. Extraction of alginate from red algae using the acid method and conversion of alginic acid to sodium alginate by adding NaOH. The impression material is made with the addition of sodium alginate. Antibacterial activity test by well diffusion method. This study consisted of 3 groups, namely control (K), red algae sodium alginate extract (P1), and impression material with red algae sodium alginate extract (P2). Groups K, P1 and P2 had an average inhibition zone of 0 mm, 15.56 mm, 17.22 mm, respectively. Kruskal Wallis and Mann Whitney test showed significant differences between groups. Impression material based on sodium alginate extract from red algae has strong antibacterial activity against L. acidophilus.
NUMBER OF BLACK-PIGMENTED ANAEROBIC BACTERIA CONTAINED IN THE GINGIVAL CREVICULAR FLUID (GCF) OF PATIENTS SUFFERING FROM THE CHRONIC PERIODONTITIS AND AGGRESSIVE PERIODONTITIS Depi Praharani; Peni Pujiastuti; Melok Aris Wahyukundari; Yuliana Mahdiyah Daat Arina; Desi Sandra Sari
Odonto : Dental Journal Vol 8, No 1 (2021): July 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (574.238 KB) | DOI: 10.30659/odj.8.1.101-107

Abstract

POTENSI BAHAN CETAK EKSTRAK NATRIUM ALGINAT RUMPUT LAUT MERAH (Kappaphycus alvarezii) DALAM MENGHAMBAT PERTUMBUHAN Streptococcus mutans Inneke Dwi Lestari; Depi Praharani; Achmad Gunadi; Izzata Barid; Didin Erma Indahyani; Niken Probosari
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Vol 10, No 1 (2023): Vol 10 No 1, Juni 2023
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33854/jbd.v10i1.1120

Abstract

Introduction: Synthetic alginate is an impression material that is widely used in dentistry. Dental impressions may carry Streptococcus mutans bacteria, which is cariogenic bacteria. Red seaweed has the potential as a source of sodium alginate and has antibacterial activity. Aim: The purpose of this study is to analyze the ability of impression materials containing sodium alginate from red seaweed in inhibiting the growth of S. mutans bacteria. Methods: This study uses a laboratory experimental method with a post-test-only control group design. The number of research samples is 24, which are divided into group A (control), group B (red seaweed sodium alginate extract), and group C (red seaweed sodium alginate extract impression material). Sodium alginate was extracted from red seaweed (Kappaphycus alvarezii) by the acid method. Inhibition test of the growth of S. mutans bacteria using well diffusion method. Growth inhibition is characterized by the formation of an inhibition zone. Result: The test results showed that group A (control) does not have the ability to inhibit the growth of S. mutans bacteria. Meanwhile, group B (RLM Na- alginate extract) and group C (RLM Na-alginate extract impression material) have the same ability to inhibit the growth of S. mutans bacteria. Conclusions: Red seaweed sodium alginate extract impression material can inhibit the growth of S. mutans
Potensi Ekstrak Daun Asam Jawa (Tamarindus indica L) dalam Menghambat Pertumbuhan Bakteri Streptococcus mutans pada Resin Akrilik Heat-cured Raissa Salma Hardiani; Achmad Gunadi; Depi Praharani; Rahardyan Parnaadji; Nadie Fatimatuzzahro
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 20 No 2 (2023)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v20i2.44012

Abstract

Heat-cured acrylic resin is a material that is often used as a denture base. However, heat-cured acrylic resin is porous which can be a site for plaque accumulation. Streptococcus mutans is a microorganism that initiates plaque formation. Therefore, the cleanliness of the denture must always be maintained, one of which is by immersing the denture in 0.5% NaOCl solution. However, NaOCl can fade the color of acrylic resin and is corrosive. Alternative materials that can be used are materials derived from nature. Tamarind leaves are known to contain bioactive compounds that have antibacterial activity. The purpose of this study is to determine the potential of tamarind leaf extract at several concentrations that could inhibit the growth of S. mutans on heat-cured acrylic resin and the concentration that had the greatest ability to inhibit the growth of S. mutans on heat-cured acrylic resin. This type of research is a laboratory experimental study with a post-test-only control group design. Each acrylic resin plate will be contaminated with S. mutans for 24 hours and then immersed in the tamarind leaves extract concentration of 3.125%; 6.25%; 12.5%; 25%, positive control (NaOCl 0,5%), and negative control. The measurement of the number of S. mutans was carried out using a spectrophotometer. . The results showed that tamarind leaf extract was able to inhibit the growth of S. mutans on heat-cured acrylic resin and the highest concentrations in inhibiting the growth of S. mutans on heat-cured acrylic resin were 12.5% and 25%.
Daya antibakteri pasta gigi yang mengandung ekstrak biji kopi robusta (Coffea canephora) terhadap Treponema denticola: eksperimental laboratoris Perdana, Mohammad Daffa Duta; Praharani, Depi; Sari, Desi Sandra
Padjadjaran Journal of Dental Researchers and Students Vol 8, No 1 (2024): Februari 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v8i1.52979

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ABSTRAK Pendahuluan: Treponema denticola merupakan bakteri anaerob Gram negatif penyebab penyakit periodontal dan dominan pada plak subgingiva. Penyakit periodontal dapat dicegah dengan pengendalian plak secara mekanis, seperti menyikat gigi dengan pasta gigi. Salah satu bahan herbal yang dapat dimanfaatkan sebagai bahan tambahan pasta gigi adalah biji kopi robusta yang mengandung senyawa aktif antibakteri. Tujuan penelitian ini adalah untuk menganalisis daya antibakteri pasta gigi yang mengandung ekstrak biji kopi robusta pada konsentrasi 12,5%, 25%, dan 50% terhadap T. denticola. Metode: Jenis penelitian yang digunakan yaitu eksperimental laboratorium dengan rancangan penelitian menggunakan the post test only control group design. Biji kopi robusta diekstraksi menggunakan metode maserasi, dan diformulasi menjadi pasta gigi dengan konsentrasi 12,5, 25 dan 50%. Uji antibakteri menggunakan metode difusi sumuran dengan media Mueller-Hinton Agar (MHA) yang telah diinokulasi T. denticola. Kelompok penelitian terdiri dari kelompok pasta gigi dengan ekstrak biji kopi robusta  konsentrasi 12,5, 25, dan 50%, kontrol positif, dan kontrol negatif. Hasil: Terbentuk zona hambat pada kelompok pasta gigi yang mengandung ekstrak biji kopi robusta konsentrasi 12,5, 25, dan 50%, dan kontrol positif terhadap pertumbuhan T. denticola. Rata-rata diameter zona hambat yang dihasilkan berturut-turut dari konsentrasi ekstrak biji kopi robusta terendah hingga tertinggi adalah 11,02 mm; 12,81 mm; dan 15,95 mm dengan perbedaan yang signifikan di antara tiap konsentrasi (p<0,05). Simpulan: Pasta gigi yang mengandung ekstrak biji kopi robusta konsentrasi 12,5, 25, dan 50% memiliki daya antibakteri terhadap T. denticola. Pasta gigi dengan konsentrasi ekstrak 50% memperlihatkan daya antibakteri yang paling besar.KATA KUNCI: biji kopi robusta, antibakteri, pasta gigi, treponema denticola, penyakit periodontalAntibacterial activity of toothpaste containing robusta coffee bean (Coffea canephora)extract towards Treponema denticola: a laboratory experimental studyABSTRACT Introduction: Treponema denticola is a Gram-negative anaerobic bacterium that causes periodontal disease and is dominant in subgingival plaque. Periodontal disease can be prevented by mechanical plaque control, such as brushing teeth with toothpaste. One of the herbal ingredients that can be used as an additional ingredient for toothpaste is robusta coffee beans which contain active antibacterial compounds. The aim of this research was to analyze the antibacterial activity of toothpaste containing Robusta coffee bean extract at concentrations of 12.5, 25, and 50% against T. denticola. Methods: The type of research used is experimental with a research design using a posttest only control group design. Robusta coffee beans were extracted using the maceration method, and formulated into toothpaste with concentrations of 12.5, 25, and 50%. The well diffusion method with Mueller-Hinton Agar (MHA) media which had been inoculated with T. denticola was used for the antibacterial activity test. The research group consisted of toothpaste with 12.5% robusta coffee bean extract group, 25% group, and 50% group, as well as the positive control and negative control groups. Results: The inhibitory zone was formed in the toothpaste with 12.5% robusta coffee bean extract group, 25% group, 50%, and positive control group against the growth of T. denticola. The average diameter of the inhibition zone produced respectively from the lowest to the highest concentration of robusta coffee bean extract were 11.02 mm, 12.81 mm, and 15.95 mm, with significant differences between concentrations (p<0,05). Conclusion: Toothpaste containing robusta coffee bean extract at concentrations of 12.5, 25, and 50% showed the antibacterial activity against T. denticola. Toothpaste containing 50% of the robusta coffee bean extract showed the greatest antibacterial activity.KEY WORDS: robusta coffee bean, antibacterial, toothpaste, treponema denticola, periodontal disease
GAMBARAN STATUS KEBERSIHAN RONGGA MULUT DAN KESEHATAN JARINGAN PERIODONTAL PADA IBU HAMIL DI WILAYAH PERKEBUNAN GLANTANGAN, KABUPATEN JEMBER Prastifa, Febi Zulaikha; Sari, Desi Sandra; Praharani, Depi
Jurnal Kesehatan Vol 17 No 1 (2024): JURNAL KESEHATAN
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/kesehatan.v17i1.35720

Abstract

Introduction: During pregnancy, there are changes in hormone levels that can affect the condition of the oral cavity. An increase in hormones leads to gingival changes and a decrease in salivary flow that can affect the hygiene of the oral cavity and periodontal tissues leading to the occurrence of local inflammation and increased plaque accumulation. A sustained increase in plaque accumulation leads to periodontal disease. The purpose of this study was to determine the hygiene status of the oral cavity and the health of the periodontal tissue of pregnant women. Method: This type of research is descriptive observational with a total sampling data collection technique involving 51 pregnant women in 8 posyandu Pondokrejo Village, Glantangan Plantation area. Data collection was obtained through the oral hygiene index simplified (OHI-S), Community Periodontal Index of Treatment Needs (CPITN), and questionnaires. Results: The results of the OHI-S examination of pregnant women were obtained at 58.8% including moderate criteria, 35.3% bad, and 5.9% good. CPITN results are healthy periodontal 0%; bleeding when probing 0%; calculus and other retentive factors 86.3%; pathological pocket 4-5 mm 11.8%; pathological pocket 6 mm 2%. Pregnant women were most in the age of 26-35 years (58.82%), 3rd trimester (41.18%), last primary education (43.14%), had one-sided chewing habits (56.86%), never visited the dentist (92.16%), never scaling (90.20%), and brushed their teeth every bath (68.63%). Conclusion: The most common oral health status in pregnant women in the glantangan plantation area is moderate. The most common periodontal tissue health status in pregnant women is the condition that it is supragingival or subgingival calculus or there are debris retentive factors such as crowns and restorations that are not adapted properly.