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Cytotoxicity analysis of alginate impression materials based red seaweed extract on cultured gingival fibroblast cells Praharani, Depi; Barid, Izzata; Indahyani, Didin Erma; Probosari, Niken; Lestari, Sri; Sulistiyani, Sulistiyani; Sumono, Agus
Odonto : Dental Journal Vol 11, No 2 (2024): December 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.2.290-297

Background: Alginate is an impression material that is widely used in dentistry. Alginate can actually also be obtained from natural materials such as red seaweed. The impression procedure causes the impression material to come into contact with oral tissues including the gingiva. Ideally, the materials used must also meet requirements such as low toxicity or non-toxicity so that tissue damage does not occur. This study aims to analyze the cytotoxicity of alginate impression materials from red seaweed extract in gingival fibroblast cell. Method: This experimental laboratory design using post-test only control group design. The research groups consisted of: sodium alginate extract group, red seaweed extract-based alginate impression material, positive control and negative control. Cytotoxicity was tested on gingival fibroblast cell cultures and the effect was analyzed using the MTT assay. Exposure to gingival fibroblast cell cultures was differentiated in three time durations: 5 minutes, 10 minutes and 15 minutes. Each time duration was repeated three times. MTT-formazan production is a method used to measure cell viability (living cells). The data obtained were statistically analyzed using two-way ANOVA test and Tukey HSD post hoc test. Result: There was no significant difference in the average cell viability between the red seaweed extract-based alginate impression material group and the negative control group at an exposure duration of 5 minutes, which was more than 90%. Conclusion: The red seaweed extract-based alginate impression material has no toxic effect on gingival fibroblast cells at 5 minutes exposure.

Cytotoxicity analysis of alginate impression materials based red seaweed extract on cultured gingival fibroblast cells Praharani, Depi; Barid, Izzata; Indahyani, Didin Erma; Probosari, Niken; Lestari, Sri; Sulistiyani, Sulistiyani; Sumono, Agus
Odonto : Dental Journal Vol 11, No 2 (2024): December 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.2.290-297

Background: Alginate is an impression material that is widely used in dentistry. Alginate can actually also be obtained from natural materials such as red seaweed. The impression procedure causes the impression material to come into contact with oral tissues including the gingiva. Ideally, the materials used must also meet requirements such as low toxicity or non-toxicity so that tissue damage does not occur. This study aims to analyze the cytotoxicity of alginate impression materials from red seaweed extract in gingival fibroblast cell. Method: This experimental laboratory design using post-test only control group design. The research groups consisted of: sodium alginate extract group, red seaweed extract-based alginate impression material, positive control and negative control. Cytotoxicity was tested on gingival fibroblast cell cultures and the effect was analyzed using the MTT assay. Exposure to gingival fibroblast cell cultures was differentiated in three time durations: 5 minutes, 10 minutes and 15 minutes. Each time duration was repeated three times. MTT-formazan production is a method used to measure cell viability (living cells). The data obtained were statistically analyzed using two-way ANOVA test and Tukey HSD post hoc test. Result: There was no significant difference in the average cell viability between the red seaweed extract-based alginate impression material group and the negative control group at an exposure duration of 5 minutes, which was more than 90%. Conclusion: The red seaweed extract-based alginate impression material has no toxic effect on gingival fibroblast cells at 5 minutes exposure.

GAMBARAN STATUS KEBERSIHAN RONGGA MULUT DAN KESEHATAN JARINGAN PERIODONTAL PADA LANSIA DI WILAYAH PERKEBUNAN RENTENG (STUDI KASUS DI DESA MANGARAN, KECAMATAN AJUNG, KABUPATEN JEMBER) Sonya Cut Yumita Putri; Peni Pujiastuti; Depi Praharani
Jurnal Kesehatan Vol 17 No 2 (2024): JURNAL KESEHATAN
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/kesehatan.v17i2.35718

Periodontal disease is an infection in the tissues supporting the teeth. In general, periodontal disease consists of gingivitis and periodontitis. The main cause of this disease is dental plaque bacteria. This disease can affect anyone, including the elderly. Periodontal disease is caused by degenerative changes and plaque accumulation over a long period of time. The purpose of this study was to determine the description of oral hygiene status and periodontal health of elderly in Renteng Plantation area (Mangaran Village, Ajung District, Jember Regency). The research method was observational descriptive cross-sectional, with 90 people divided into two groups of 60–69 years and 70 years. The examinations included oral hygiene with OHI-S and periodontal tissue health with CPITN are presented in tables and figures. The results showed that the level of oral hygiene based on the OHI-S score was 1 person (1.1%) classified as good, 14 people (15.6%) classified as moderate, and 75 people (83.3%) classified as poor. In examining the health status of periodontal tissue based on the CPITN score, 46 people (51.1%) had score of 2, 40 people (44.4%) had score of 3, and 4 people (4.5%) had score of 4. No one had CPITN scores of 0 and 1. The conclusion is that the oral hygiene status of the elderly in the joint plantation area mostly classified as poor at 83.3%. Meanwhile, the health status of the periodontal tissue was mostly found to have supragingival or subgingival calculus at 51.1%.

Kemampuan Pasta Gigi yang Mengandung Ekstrak Biji Kopi Robusta (Coffea canephora) dalam Menghambat Pertumbuhan Bakteri Fusobacterium nucleatum Vindy. W, Imaniar Fitriatasya; Pujiastuti, Peni; Praharani, Depi
Pustaka Kesehatan Vol. 13 No. 1 (2025): Volume 13 No. 1, 2025
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Fusobacterium nucleatum is a gram-negative obligate anaerobic bacterium that is most dominantly found in the oral cavity and plays an important role in periodontitis pathology. Periodontitis can be prevented by controlling plaque, namely, brushing teeth. Therefore, toothpaste needs to be optimized by adding an antibacterial substance. The robusta coffee (Coffea canephora) contains caffeine, phenols, trigonelline, chlorogenic acid, saponins, tannins, and triterpenoids, which have proven to be antibacterial. This study aimed to assess the ability of toothpaste containing robusta coffee bean extract to inhibit the growth of F. nucleatum. Robusta coffee beans were extracted using the maceration method and formulated into toothpaste with concentrations of 12.5%, 25%, and 50%. Antibacterial tests were carried out by the well diffusion method on MHA media that had been inoculated with F. nucleatum and the measured the diameter of the inhibition zone. The result showed that there was a significant difference in the inhibition zone diameter between the group positive control, the negative control, and toothpaste containing robusta coffee bean extract 12.5%, 25%, and 50%. The largest inhibition zone formed consecutively was in toothpaste containing robusta coffee bean extract 50%, 25%, and 12,5%. It can be concluded that toothpaste containing robusta coffee bean extract with concentrations of 12.5%, 25%, and 50% can inhibit the growth of F. nucleatum.

NUMBER OF BLACK-PIGMENTED ANAEROBIC BACTERIA CONTAINED IN THE GINGIVAL CREVICULAR FLUID (GCF) OF PATIENTS SUFFERING FROM THE CHRONIC PERIODONTITIS AND AGGRESSIVE PERIODONTITIS Praharani, Depi; Pujiastuti, Peni; Wahyukundari, Melok Aris; Daat Arina, Yuliana Mahdiyah; Sari, Desi Sandra
Odonto : Dental Journal Vol 8, No 1 (2021): July 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Analisis cemaran logam berat dalam pasta gigi ekstrak biji kopi robusta Pujiastuti, Peni; Arina, Yuliana Mahdiyah Da'at; Praharani, Depi; Wahyukundari, Melok Aris; Sakinah, Neira Najatus; Sari, Desi Sandra
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 37, No 3 (2025): Desember 2025
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v37i3.61265

Pendahuluan: Pasta gigi bermanfaat dalam upaya kontrol plak secara mekanis sehingga berperan dalam mencegah penyakit periodontal. Produk pasta gigi ekstrak biji kopi robusta dalam pasta gigi harus memenuhi Standar Nasional Indonesia (SNI) 8861:2020, diantaranya cemaran logam berat, Timbal (Pb), Cadmium (Cd), Raksa (Hg) dan Arsen (As) dapat mengganggu kesehatan tubuh manusia. Belum ada informasi tentang adanya cemaran logam berat dalam pasta gigi ekstrak biji kopi robusta. Tujuan penelitian mengkaji kesesuaian cemaran Pb, Cd, Hg dan As dalam pasta gigi ekstrak biji kopi robusta dengan standar SNI 8861:2020. Metode: Jenis penelitian ini eksperimental laboratoris dengan sampel pasta gigi ekstrak biji kopi robusta dengan konsentrasi 0,0625%, 0,125%, 0,25%, dan 0,5% serta plasebo. Pembuatan ekstrak biji kopi robusta menggunakan metode maserasi. Pembuatan pasta gigi plasebo dengan kandungan magnesium karbonat, kalsium karbonat, gliserin, propilen glikol, trietanolamin, oleum menthae piperithae, dan aquades steril. Pembuatan pasta gigi ekstrak biji kopi robusta dengan cara mencampur bahan pasta gigi plasebo dengan ekstrak biji kopi robusta 0,0625%, 0,125%, 0,25%, dan 0,5%. Selanjutnya dilakukan uji Pb, Cd, Hg dan As dengan menggunakan metode spektrofotometri serapan atom. Hasil: Kadar Pb pada kontrol negatif 1,382 dan perlakuan 1,687–1,846. Kadar Cd pada kontrol negatif 0,240 dan perlakuan 0,216–0,239. Kadar Hg dan As pada semua kelompok 0. Seluruh kadar logam masih di bawah batas cemaran. Simpulan: Terdapat cemaran logam berat Pb dan Cd pada pasta gigi ekstrak biji kopi robusta dibawah batas maksimal berdasarkan SNI 8861:2020.Analysis of the compliance of heavy metal contamination in robusta coffee bean extract toothpaste based on the Indonesian National Standard (SNI) 8861:2020: an experimental laboratory studyIntroduction: Toothpaste plays an important role in mechanical plaque control and in preventing periodontal disease. Natural ingredients, including robusta coffee beans, have been used in toothpaste formulations. Toothpaste products containing robusta coffee bean extract must meet the Indonesian National Standard (SNI) 8861:2020, which includes provisions on heavy metal contamination (Pb, Cd, Hg, and As) that can affect human health. To date, there is no information available regarding the presence of heavy metal contamination in robusta coffee bean extract toothpaste. The purpose of the study was to evaluate the compliance of Pb, Cd, Hg and As contamination levels in robusta coffee bean extract toothpaste with the SNI 8861:2020 standard. Method: This study employed an experimental laboratory design using samples of robusta coffee bean extract toothpaste with concentrations of 0.0625%, 0.125%, 0.25%, and 0.5%, as well as a placebo. The robusta coffee bean extract was prepared using the maceration method. The placebo toothpaste was composed of magnesium carbonate, calcium carbonate, glycerin, propylene glycol, triethanolamine, oleum menthae piperitae, and sterile distilled water. The robusta coffee bean extract toothpaste was made by mixing the placebo base with the extract at concentrations of 0.0625%, 0.125%, 0.25%, and 0.5%. Subsequently, tests for Pb, Cd, Hg, and As contamination were carried out using atomic absorption spectrophotometry (AAS). Result: Pb levels in the negative control group were 1.382, while those in treatment groups ranged from 1.687 to 1.846. Cd levels in the negative control were 0.240, and in the treatment groups ranged from 0.216 to 0.239. Hg and As levels in all groups were 0. All measured levels of heavy metals remained below the contamination limits set by SNI 8861:2020. Conclusion: The findings showed the presence of Pb and Cd contamination in robusta coffee bean extract toothpaste. However, all concentrations were below the maximum permissible limits established in SNI 8861:2020.

NUMBER OF BLACK-PIGMENTED ANAEROBIC BACTERIA CONTAINED IN THE GINGIVAL CREVICULAR FLUID (GCF) OF PATIENTS SUFFERING FROM THE CHRONIC PERIODONTITIS AND AGGRESSIVE PERIODONTITIS Praharani, Depi; Pujiastuti, Peni; Wahyukundari, Melok Aris; Daat Arina, Yuliana Mahdiyah; Sari, Desi Sandra
Odonto : Dental Journal Vol 8, No 1 (2021): July 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.8.1.101-107

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