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Elastic Recovery Properties of Alginate Impression Materials Based on Red Algae Kappaphycus alvarezii Indahyani, Didin Erma; Barid, Izzata; Nafisah, Nafisah; Praharani, Depi; Sumono, Agus; Probosari, Niken; Sulistiyani, Sulistiyani
Journal of Dentistry Indonesia Vol. 30, No. 3
Publisher : UI Scholars Hub

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Alginate impression materials in dentistry are used to form details of the oral cavity organs. The alginate content in the impression material functions to form a gel. One source of alginate is algae. Objective: This study aims to determine the recovery elasticity and setting time of alginate impression materials made from red algae K. alvarezii. Methods: The sodium alginate in the composition of this alginate impression material was extracted from red algae K. alvarezii. The alginate impression material was tested for setting time and recovery elasticity and compared with commercial impression material (Hygedent®). Results: The elastic recovery of red algae sodium alginate impression materials was 97.43% which was not significantly different from the commercial impression materials (98.42%). These results showed that this alginate impression material has the same properties as commercial impression materials. In the setting time test, the the red algal alginate impression material was much longer than the commercial impression material, 1.39 m and 3.30 m respectively. Conclusion: It was concluded that the alginate impression material from the red algae K. alvarezii had a long setting time. At the same time, the recovery of elasticity did not differ from that of commercially available alginate impression materials.

Potensi Pasta Gigi Ekstrak Etanol Biji Hijau Kopi Robusta (Coffea Canephora) dalam Menghambat Pertumbuhan Streptococcus Mitis Miftakhur Rizki; Melok Aris Wahyukundari; Depi Praharani
Cakradonya Dental Journal Vol 16, No 2 (2024): Agustus 2024
Publisher : FKG Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/cdj.v16i2.37122

Salah satu bakteri koloni primer yang membentuk plak gigi adalah Streptococcus mitis yang memiliki wall-associated adhesin yang membantu perlekatan dan kolonisasi. Plak gigi yang terus terakumulasi menyebabkan penyakit infeksi rongga mulut seperti penyakit periodontal dan karies Untuk pencegahan akumulasi plak, dilakukan kegiatan kontrol plak, salah satunya dengan menyikat gigi yang dibantu pasta gigi. Pasta gigi mengandung berbagai bahan aktif termasuk bahan antibakteri. Ekstrak etanol biji hijau kopi robusta (Coffea canephora) memiliki kandungan antibakteri seperti kafein, trigonelin, asam klorogenat, flavonoid, saponin, dan terpenoid. Tujuan penelitian ini adalah untuk menganalisis potensi pasta gigi yang mengandung ekstrak etanol biji hijau kopi robusta konsentrasi 12,5%, 25%, dan 50% dalam menghambat pertumbuhan S. mitis. Jenis penelitian yaitu penelitian eksperimental laboratoris secara in vitro dengan rancangan the post-test-only control group design. Pengujian potensi antibakteri dilakukan dengan agar disk-diffusion test pada media yang telah diinokulasi bakteri S. mitis dengan 5 kelompok sampel dan 4 kali pengulangan. Pengukuran zona hambat setelah 24 jam inkubasi. Data kemudian dianalisis menggunakan SPSS. Diperoleh kesimpulan bahwa pasta gigi yang mengandung ekstrak etanol biji hijau kopi robusta 12,5%, 25%, dan 50% memiliki potensi dalam menghambat pertumbuhan S. mitis, serta terdapat hubungan antara konsentrasi ekstrak yang diformulasikan dengan besar zona hambat yang dihasilkan.

Cytotoxicity analysis of alginate impression materials based red seaweed extract on cultured gingival fibroblast cells Praharani, Depi; Barid, Izzata; Indahyani, Didin Erma; Probosari, Niken; Lestari, Sri; Sulistiyani, Sulistiyani; Sumono, Agus
Odonto : Dental Journal Vol 11, No 2 (2024): December 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.2.290-297

Background: Alginate is an impression material that is widely used in dentistry. Alginate can actually also be obtained from natural materials such as red seaweed. The impression procedure causes the impression material to come into contact with oral tissues including the gingiva. Ideally, the materials used must also meet requirements such as low toxicity or non-toxicity so that tissue damage does not occur. This study aims to analyze the cytotoxicity of alginate impression materials from red seaweed extract in gingival fibroblast cell. Method: This experimental laboratory design using post-test only control group design. The research groups consisted of: sodium alginate extract group, red seaweed extract-based alginate impression material, positive control and negative control. Cytotoxicity was tested on gingival fibroblast cell cultures and the effect was analyzed using the MTT assay. Exposure to gingival fibroblast cell cultures was differentiated in three time durations: 5 minutes, 10 minutes and 15 minutes. Each time duration was repeated three times. MTT-formazan production is a method used to measure cell viability (living cells). The data obtained were statistically analyzed using two-way ANOVA test and Tukey HSD post hoc test. Result: There was no significant difference in the average cell viability between the red seaweed extract-based alginate impression material group and the negative control group at an exposure duration of 5 minutes, which was more than 90%. Conclusion: The red seaweed extract-based alginate impression material has no toxic effect on gingival fibroblast cells at 5 minutes exposure.

Cytotoxicity analysis of alginate impression materials based red seaweed extract on cultured gingival fibroblast cells Praharani, Depi; Barid, Izzata; Indahyani, Didin Erma; Probosari, Niken; Lestari, Sri; Sulistiyani, Sulistiyani; Sumono, Agus
Odonto : Dental Journal Vol 11, No 2 (2024): December 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.2.290-297

Background: Alginate is an impression material that is widely used in dentistry. Alginate can actually also be obtained from natural materials such as red seaweed. The impression procedure causes the impression material to come into contact with oral tissues including the gingiva. Ideally, the materials used must also meet requirements such as low toxicity or non-toxicity so that tissue damage does not occur. This study aims to analyze the cytotoxicity of alginate impression materials from red seaweed extract in gingival fibroblast cell. Method: This experimental laboratory design using post-test only control group design. The research groups consisted of: sodium alginate extract group, red seaweed extract-based alginate impression material, positive control and negative control. Cytotoxicity was tested on gingival fibroblast cell cultures and the effect was analyzed using the MTT assay. Exposure to gingival fibroblast cell cultures was differentiated in three time durations: 5 minutes, 10 minutes and 15 minutes. Each time duration was repeated three times. MTT-formazan production is a method used to measure cell viability (living cells). The data obtained were statistically analyzed using two-way ANOVA test and Tukey HSD post hoc test. Result: There was no significant difference in the average cell viability between the red seaweed extract-based alginate impression material group and the negative control group at an exposure duration of 5 minutes, which was more than 90%. Conclusion: The red seaweed extract-based alginate impression material has no toxic effect on gingival fibroblast cells at 5 minutes exposure.

GAMBARAN STATUS KEBERSIHAN RONGGA MULUT DAN KESEHATAN JARINGAN PERIODONTAL PADA LANSIA DI WILAYAH PERKEBUNAN RENTENG (STUDI KASUS DI DESA MANGARAN, KECAMATAN AJUNG, KABUPATEN JEMBER) Sonya Cut Yumita Putri; Peni Pujiastuti; Depi Praharani
Jurnal Kesehatan Vol 17 No 2 (2024): JURNAL KESEHATAN
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/kesehatan.v17i2.35718

Periodontal disease is an infection in the tissues supporting the teeth. In general, periodontal disease consists of gingivitis and periodontitis. The main cause of this disease is dental plaque bacteria. This disease can affect anyone, including the elderly. Periodontal disease is caused by degenerative changes and plaque accumulation over a long period of time. The purpose of this study was to determine the description of oral hygiene status and periodontal health of elderly in Renteng Plantation area (Mangaran Village, Ajung District, Jember Regency). The research method was observational descriptive cross-sectional, with 90 people divided into two groups of 60–69 years and 70 years. The examinations included oral hygiene with OHI-S and periodontal tissue health with CPITN are presented in tables and figures. The results showed that the level of oral hygiene based on the OHI-S score was 1 person (1.1%) classified as good, 14 people (15.6%) classified as moderate, and 75 people (83.3%) classified as poor. In examining the health status of periodontal tissue based on the CPITN score, 46 people (51.1%) had score of 2, 40 people (44.4%) had score of 3, and 4 people (4.5%) had score of 4. No one had CPITN scores of 0 and 1. The conclusion is that the oral hygiene status of the elderly in the joint plantation area mostly classified as poor at 83.3%. Meanwhile, the health status of the periodontal tissue was mostly found to have supragingival or subgingival calculus at 51.1%.

Kemampuan Pasta Gigi yang Mengandung Ekstrak Biji Kopi Robusta (Coffea canephora) dalam Menghambat Pertumbuhan Bakteri Fusobacterium nucleatum Vindy. W, Imaniar Fitriatasya; Pujiastuti, Peni; Praharani, Depi
Pustaka Kesehatan Vol. 13 No. 1 (2025): Volume 13 No. 1, 2025
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Fusobacterium nucleatum is a gram-negative obligate anaerobic bacterium that is most dominantly found in the oral cavity and plays an important role in periodontitis pathology. Periodontitis can be prevented by controlling plaque, namely, brushing teeth. Therefore, toothpaste needs to be optimized by adding an antibacterial substance. The robusta coffee (Coffea canephora) contains caffeine, phenols, trigonelline, chlorogenic acid, saponins, tannins, and triterpenoids, which have proven to be antibacterial. This study aimed to assess the ability of toothpaste containing robusta coffee bean extract to inhibit the growth of F. nucleatum. Robusta coffee beans were extracted using the maceration method and formulated into toothpaste with concentrations of 12.5%, 25%, and 50%. Antibacterial tests were carried out by the well diffusion method on MHA media that had been inoculated with F. nucleatum and the measured the diameter of the inhibition zone. The result showed that there was a significant difference in the inhibition zone diameter between the group positive control, the negative control, and toothpaste containing robusta coffee bean extract 12.5%, 25%, and 50%. The largest inhibition zone formed consecutively was in toothpaste containing robusta coffee bean extract 50%, 25%, and 12,5%. It can be concluded that toothpaste containing robusta coffee bean extract with concentrations of 12.5%, 25%, and 50% can inhibit the growth of F. nucleatum.

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