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All Journal HAYATI Journal of Biosciences ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Journal of Marine Research Jurnal Kelautan Tropis JURNAL ENGGANO Journal of Fisheries & Marine
Rini Pramesti
Department Of Marine Sciences, Faculty Of Fisheries And Marine Sciences, Diponegoro University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Other

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Analisis Kualitas Alginat Rumput Laut (Padina sp.) Menggunakan Metode Ekstraksi Jalur Kalsium Pasaribu, Adam Sojuangon; Sedjati, Sri; Pramesti, Rini
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (854.997 KB) | DOI: 10.14710/jmr.v9i1.25502

Abstract

ABSTRAK: Padina sp. merupakan spesies rumput laut cokelat (Phaeophyta) penghasil alginat, namun kualitas alginat yang dihasilkan tergolong rendah dibandingkan alginofit lainnya. Metode ekstraksi jalur kalsium diduga dapat meningkatkan kualitas alginat sehingga penelitian ini perlu dilakukan. Tujuan penelitian ini untuk mengkaji apakah ekstraksi jalur kalsium dapat meningkatkan mutu alginat yang dihasilkan dari rumput laut Padina. Ekstraksi dilakukan dengan metode jalur kalsium konvensional dan jalur kalsium MAE (Microvawe Assisted Extraction). Metode penelitian yang digunakan adalah eksperimental laboratoris dengan 1 perlakuan (kosentrasi CaCl2) dan 3 taraf konsentrasi yaitu 0,5 M 1,0 M dan 1,5 M. Parameter kualitas yang dianalisis meliputi rendemen, kadar air, Kadar abu, viskositas, pH dan FTIR. Hasil uji two way ANOVA menunjukan terdapat signifikansi rendemen dengan nilai 0,007. Metode jalur kalsium MAE dengan konsentrasi 1,0 M CaCl2 menghasilkan kualitas terbaik dengan nilai rendemen (26,83%), kadar air (10,67%), kadar abu (21,83%), viskositas (11,42 cPs)  dan pH (6,77). Hasil analisis FTIR menunjukan ke-2 metode ekstraksi menghasilkan natrium alginat dengan adanya sidik jari guluronat dan manuronat pada bilangan gelombang 930-890 cm-1 dan 870-820 cm-1. ABSTRACT: Padina sp. is a species of brown seaweed (Phaephyta) producing alginates, but the quality of alginates produced is relatively low compared to other alginophytes. The calcium pathway extraction method is thought to improve the quality of alginates thus the reason of the research. This research aims to examine whether the calcium pathway extraction improves the quality of alginates produced from Padina. Seaweed extraction was carried out using conventional calcium pathways and MAE (Microwave Assisted Extraction) calcium pathways. The research method used was laboratory experimental with 1 treatment (CaCl2 concentration) and 3 concentration levels : 0.5 M, 1.0 M, 1.5 M. Quality parameters analyzed includes yield, moisture content, ash contetnt, viscosity, pH and FTIR. The two way ANOVA test results showed that there was a significance of yield value of 0.007. The MAE calcium pathway method with a concentration of 1.0 M CaCl2 produces the best quality with a yield value (26.83%), water content (10.67%), ash content (21.83%), viscosity (11.42 cPs) and pH (6.77). FTIR analysis results show that the two extraction methods produces sodium alginate in the presence of guluronate and manuronic fingerprints at wave numbers 930-890 cm-1 and 870-820 cm-1.
KAJIAN PEMANFAATAN LIMBAH PADAT INDUSTRI PENGOLAHAN AGARAGAR KERTAS BERBAHAN BAKU RUMPUT LAUT Gracilaria sp. SEBAGAI PUPUK PADA TANAMAN BAYAM (Amaranthus sp.) Adiguna, Galih S.; Pramesti, Rini; Susanto, A. B.
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.911 KB) | DOI: 10.14710/jmr.v3i1.4595

Abstract

Study the utilization of solid waste seaweed Gracilaria sp. processing results agar paper as fertilizer on plant spinach (Amaranthus sp .) has been done. Case studies in Agar-Agar Paper Factory Cap Apples in the Village Mancagahar, Pamengpeuk District, Garut regency. Was comparing parameters with seaweed solid waste compost on the market and testing this on the test plant spinach (Amaranthus sp .). Results of chemical analysis of seaweed solid waste generated from agar paper processing is pH 7.3 (neutral), COrganic 27.28 %; moisture content 29.98 % ; ratio C / N 8, N-total 3.42 % ; Phosphorus 0.37%, Ca 10.44%, Mg 0.98 %, 1.54 % K, 1.70% Na; 2573 ppm Fe; 5 ppm Cu; 182 ppm Zn; Mn 83 ppm; KTK 49.33 cmol / kg; KB> 100%. These results meet the minimum technical requirements of organic fertilizer. Optimal results on plant growth test using the media of waste, waste treatment that achieved by 15% , while the use of compost to be achieved in compost treatment 25%. Solid waste of seaweed has the potential as spinach (Amaranthus sp .) plant fertilizer.
Logam Pb pada Avicennia marina Forssk, 1844 (Angiosperms : Acanthaceae) di Lingkungan Air, Sedimen, di Pesisir Timur Semarang Testi, Ega Hagita; Soenardjo, Nirwani; Pramesti, Rini
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (560.838 KB) | DOI: 10.14710/jmr.v8i2.25212

Abstract

Akar Avicennia marina merupakan bagian yang pertama terpapar logam berat timbal. Akar ini menyerap dan menyebarkan keseluruh bagian tanaman. Tujuan dari penelitian ini untuk mengkaji kandungan logam berat Pb dalam akar dan daun A. marina disekitar Kawasan Perairan Industri Terboyo, Semarang. Metode yang digunakan adalah metode deskriptif dan penentuan lokasi dengan metode purposive sampling. Pengambilan sampel meliputi air, sedimen, akar, dan daun mangrove di sepanjang aliran Sungai Sringin, Sungai Babon, dan Sungai Tenggang. Analisis kandungan logam berat di air dilakukan di Laboratorium Balai Lingkungan Hidup (BLH). Analisis kandungan logam berat di sedimen, akar, daun muda dan daun tua dilakukan di Laboratorium Balai Besar Teknologi Pencegahan Pencemaran Industri (BBTPPI) dengan menggunakan Atomic Absorbtion Spectrophotometry (AAS). Kandungan logam berat dalam air <0,00-0,01 mg/L, sedimen <0,03 - 6,23 mg/kg, akar 0,20-0,31 mg/kg, daun muda 0,10-0,13 mg/kg, dan daun tua 0,10-0,15 mg/kg. Hasil penelitian dapat disimpulkan bahwa kandungan logam berat di air diatas baku mutu (KepMen LH No. 51, 2004) sebesar 0,008 mg/L sedangkan sedimen dibawah baku mutu (National Oceanic and Atmospheric Administration, 1999) sebesar 30,24 ppm. ABSTRACT : The roots of Avicennia marina was a plant that can be accumulated by heavy metals. This  plant roots were first exposed to heavy metals, especially heavy metals Pb, was the root. The roots would absorb and spread in all parts of the plant This study was aimed to examine the heavy metal content of Pb in the roots and leaves of A. marina around Area of Terboyo Industrial Water, Semarang. The method used in this research was descriptive method and the location decision with  purposive sampling method. The sampling included were water, sediments, roots, mangrove leaves along Sringin River, Babon River, and Tenggang River. The analysis of heavy metal content in the water was performed in the Laboratory of Environment (BLH). The analysis of heavy metal content in the sediment, the roots, young leaves and old leaves were conducted in Laboratory Technology Center Industrial Pollution Prevention (BBTPPI) using Atomic Absorption Spectrophotometry (AAS). Heavy Metal contained in water < 0,00 to  0,01 mg/L, sediment < 0,030 to 6,23 mg/kg, the roots of A. marina were 0,20 to 0,31 mg/kg, the young leaves were 0,10 to 0,13 mg/kg, and the old leaves 0,10 to 0,15 mg/kg. the result of this research can be concluded that the heavy metal content  in water were high quality standard (KepMen LH No. 51, 2004) of 0,008 mg/L and in sediments below was
Produksi Bioetanol Dari Tepung Agar Gracilaria verrucosa (Hudson) Papenfuss yang Dihidrolisis Dengan Menggunakan Larutan Asam Sulfat Saputra, Wahid Agung; Susanto, AB; Pramesti, Rini
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.485 KB) | DOI: 10.14710/jmr.v2i2.2346

Abstract

Fossil fuels are non-renewable fuels and overexploited. It has an impact on depletion oil reserves so needed renewable energy such as bio-ethanol. Bioethanol is alternative fuel made from biomass containing component sugars, starches, and cellulose. Agar is polysaccharide in cell wall seaweed Gracilaria verrucosa. Polysaccharides can be hydrolyzed with chemically or enzymatically into monosaccharides and then be fermented into bioethanol. The purpose of this study was to determine the potential of powder agar for bioethanol feedstock; optimum sulfuric acid to hydrolyze powder agar, and knowing optimum fermentation time on ethanol production. The study was conducted from February to June 2012 in Microbiology Laboratory, Research Center for Biology-LIPI, Cibinong. Powder agar is obtained from manufacturers in Malang. Yeast Saccharomyces cerevisiae is a collection of LIPI-MC. Reducing sugar content was analyzed by the method of Miller (1959), while concentration ethanol were analyzed by Gas Chromatography (GC). Method used in this study is an experimental laboratory for the hydrolysis and repeatable measurements for fermentation processes. Hydrolysis process using concentration H2SO4 of 0M, 0.1 M, 0.3 M, and 0.5 M. Fermentation using yeast S. cerevisiae with the incubation time 120 hours. Results showed powder agar can be used as raw material for bioethanol. Optimum reduction sugar 35.38 mg/ml ± 0.59 obtained in H2SO4 0.3 M. Ethanol Optimum 0.77%, obtained at 120 hours incubation.
Aktivitas Antibakteri Ekstrak Metanol Rumput Laut Gracilaria verrucosa, Greville, 1830 (Florideophyceae : Gracilariaceae) di Balai Besar Perikanan Budidaya Air Payau Jepara Widodo, Radhian Wikanarto; Subagiyo, Subagiyo; Pramesti, Rini
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (533.735 KB) | DOI: 10.14710/jmr.v8i3.25271

Abstract

Infeksi bakteri patogen menjadi masalah kesehatan dunia. Antibiotik untuk penanggulangan infeksi menjadikan bakteri resisten karena pemakaiannya tidak sesuai dosis. Permasalahan ini dapat diatasi salah satunya dengan pencarian sumber antibiotik baru termasuk diantaranya pada rumput laut. Penelitian ini bertujuan untuk menentukan aktivitas antibakteri ekstrak metanol G. verrucosa terhadap Escherichia coli, Bacillus cereus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Vibrio anguillarum, dan Vibrio alginolyticus. Penelitian dilakukan dengan metode eksperimental laboratoris. Sampel rumput laut hasil budidaya Balai Besar Perikanan Budidaya Air Payau (BBPBAP) Jepara dikeringkan kemudian dimaserasi dengan metanol selama 2x24 jam. Aktivitas antibakteri ekstrak diuji pada konsentrasi 100 µg/disk, 50 µg/disk, 25 µg/disk, dan 10 µg/disk kemudian dilakukan uji fitokimia. Hasil uji aktivitas antibakteri menunjukkan bahwa ekstrak metanol G. verrucosa tidak memiliki aktivitas antibakteri. Uji fitokimia menunjukkan ekstrak mengandung flavonoid, saponin, steroid, dan triterpenoid. Pathogenic bacterial infection become a world health problem. Antibiotics for prevention of infection make bacteria resistant due to the incorrect dosage. One of these problems can be overcome by finding a new source of antibiotics including seaweed. This study aims to determine the antibacterial activity of methanol extract of G. verrucosa against Escherichia coli, Bacillus cereus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Vibrio anguillarum, and Vibrio alginolyticus. Laboratory experimental method was used in this study. Seaweed samples from the cultivation at Brackish Water Aquaculture Center (BBPBAP) Jepara were dried and then macerated with methanol for 2x24 hours. The antibacterial activity of the extract was tested at concentration of 100 µg / disk, 50 µg / disk, 25 µg / disk, and 10 µg / disk then phytochemical tests were carried out. The results of antibacterial activity test showed that G. verrucosa methanol extract did not have antibacterial activity. The phytochemical test showed that seaweed extract contained flavonoids, saponins, steroids, and triterpenoids
Aktivitas Antioksidan Rumput Laut Acanthophora muscoides (Linnaeus) Bory Dari Pantai Krakal Gunung Kidul Yogyakarta Akbar, Muhamad Rahadian; Pramesti, Rini; Ridlo, Ali
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (543.815 KB) | DOI: 10.14710/jmr.v7i1.25882

Abstract

ABSTRAK : Acanthophora muscoides merupakan salah satu rumput laut merah yang berpotensi sebagai senyawa bioaktif yang dapat digunakan sebagai antioksidan alami. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan ekstrak metanol dan n-heksan, kadar total fenolat serta kadar pigmen (klorofil a & karotenoid) dari sampel segar A. muscoides. Penentuan aktivitas antioksidan dilakukan menggunakan metode transfer elektron dengan DPPH (1,1-diphenyl-2-picrylhidrazyl) sebagai radikal bebas. Penentuan kadar total fenolat menggunakan reagen Folin-Ciocalteu dengan asam galat sebagai standar dan penentuan kandungan pigmen menggunakan prinsip spektofotometri pada λ 663 nm, 646 nm dan 470 nm. Hasil penelitian menunjukkan A. muscoides memiliki aktivitas antioksidan sangat lemah dengan nilai IC50 ekstrak metanol 325,47 ppm dan ekstrak n-heksan 351,27 ppm, sedangkan kadar total fenolat pada masing-masing ekstrak 22,68 dan 46,19 (mg GAE/g ekstrak), kadar klorofil a 7,72 dan 24,93 (mg/g sampel), dan kadar karotenoid 28,52 dan 68,55 (µ mol/g sampel).   ABSTRACT : Acanthophora muscoides is one of the red seaweed that is potentially used as a natural antioxidant. This study aims to determine the antioxidant activity of methanol and n-hexane extract, and determine the total phenolic content and levels of pigments (chlorophyll and carotenoid) of fresh samples A. muscoides. Antioxidant activity measured using electron transfer method with DPPH (1,1-diphenyl-2-picrylhidrazyl) as free radicals, while the determination of total phenolic content using the Folin-Ciocalteu reagent with gallic acid as standard and determination of pigment content measured by spectrophotometer at  a wavelength of 663 nm, 646 nm and 470 nm. The result showed IC50 value of methanol extract was 325.47 ppm and n-hexane extract was 351.27 ppm which means that A. muscoides have very weak antioxidant activity. Total phenolic content in each extract were 22.68 and 46.19 (mg GAE/g extract), chlorophyll a 7.72 and 24.93 (mg/g sample) and carotenoid 28.52 and 68.55 (μ mol/g sample).
Produktivitas Dan Dekomposisi Serasah Daun Mangrove Di Kawasan Vegetasi Mangrove Pasar Banggi, Rembang - Jawa Tengah Leksono, Satria Sakti Budi; Soenardjo, Nirwani; Pramesti, Rini
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.679 KB) | DOI: 10.14710/jmr.v3i4.11414

Abstract

Produksi serasah mangrove merupakan bagian yang penting dalam transfer bahan organik, karena unsur hara yang dihasilkan dari proses dekomposisi serasah sangat penting dalam pertumbuhan mangrove dan sebagai sumber detritus bagi ekosistem laut dan estuari. Penelitian ini bertujuan untuk mengkaji tingkat produktivitas serasah dan kecepatan dekomposisi serasah daun mangrove di kawasan vegetasi mangrove Pasar Banggi. Hasil penelitian menunjukan bahwa rerata total produksi serasah sebesar 617,34 gr/100 m/2 minggu. Daun merupakan penyumbang terbanyak rerata total produksi serasah dan Rhizopora mucronata. lebih banyak menyumbangkan serasah dibandingkan dengan Soneratia alba. Sedangkan pada pengamatan proses R. mucronata terdekomposisi lebih cepat dibandingkan S. alba
Korelasi Kandungan Logam Berat Pb dalam Air terhadap Daun Lamun Thalassia hemprichii (Ehrenberg) Ascherson 1871 (Magnoliopsida: Hydrocharitaceae) di Perairan Pulau Panjang dan Pantai Bandengan, Jepara Istiqomahani, Dara Ramadhania; Suryono, Chrisna Adhi; Pramesti, Rini
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.364 KB) | DOI: 10.14710/jmr.v9i2.27441

Abstract

 ABSTRAK : Kegiatan manusia meliputi pertanian, industri, mebel, pariwisata dan kegiatan nelayan di Pulau Panjang dan Pantai Bandengan diduga menjadi sumber logam berat Pb. Tujuan penelitian ini adalah mengetahui nilai konsentrasi dan hubungan kandungan logam berat Pb pada Air dan daun lamun Thalassia hemprichii di Pulau Panjang dan Pantai Bandengan, Jepara Materi yang digunakan dalam penelitian ini adalah sampel air kedua perairan dan daun lamun T. hemprichi. Hasil penelitian menunjukan. Konsentrasi Pb dalam air laut yang ada di kedua daerah tersebut rata rata antara 0,034–0,054 ppm sedangkan dalam daun lamun 0,509-1,334 ppm.  Korelasi antara konsentrasi logam berat Pb dalam air dan daun lamun T. hemprichii menunjukan tingginya keeratan hubungan atara dua faktor tersebut. Bila konsentrasi Pb dalam air laut meningkat akan diikuti dengan peningkatan konsentrasi Pb dalam daun lamun dengan sangat nyata.ABSTACK : Human activities include agriculture, industry, tourism and fishing activities in Panjang Island an Bandengan shores of the supposedly heavy metal Pb (lead). The purpose of this researches to compare the heavy metal content of Pb (lead) in water and leaves Thalassia hemprichii in Panjang Island an Bandengan shore, based on existing quality. The material used in this researched were the samples of leaves of T. hemprichii and water of both. The result showed that the concentration of Pb in marine waters on two regions between 0,034–0,054 ppm and concentration in seagrass leaves between 0,509-1,334 ppm. The result of the correlation between the concentration of heavy metals Pb in water and leaves of seagrass showed a high close relationship between the two factors. If the Pb concentration in seawater rises, it will be followed by an increase in Pb concentration in leaves of seagrass very significantly.
Isolasi Glukosamin dari Limbah Cangkang Rajungan Portunus pelagicus (Linnaeus,1758) (Malacostraca:Portunidae) dengan Hidrolisis Asam Klorida Ghofari, Miftahul Akhyar; Ridlo, Ali; Pramesti, Rini
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1548.576 KB) | DOI: 10.14710/jmr.v9i2.26705

Abstract

ABSTRAK: Glukosamin merupakan monomer dari kitosan yang dapat diperoleh dari  limbah  cangkang rajungan (P pelagicus). Glukosamin dibutuhkan  dalam pembentukan dan perbaikan tulang rawan dan jaringan tubuh lainnya. Penelitian ini bertujuan untuk mengetahui karakteristik glukosamin dari limbah cangkang rajungan. Proses isolasi kitosan rajungan terdiri dari deproteinasi dengan NaOH 3N, demineralisasi dengan HCl 1N, dan deasetilasi dengan NaOH 50%. Kitosan yang diperoleh dianalisis karakteristik dan derajat deasetilasinya, selanjutnya kitosan dihidrolisis secara kimia dengan larutan HCl 20% pada suhu kamar selama 4 jam. Glukosamin yang dihasilkan dihitung rendemen, loss on drying (LoD), tingkat kelarutan dan derajat deasetilasinya. Hasil penelitian menunjukan rendemen kitosan cangkang rajungan adalah 11,3%, berwarna putih, tidak berbau, kadar air 9,2%, kadar abu 5,4%, dan derajat deasetilisasi 90,8%. Rendemen glukosamin sebesar 8,6%, dengan nilai Loss on Drying 1,3%, kelarutan sebesar 72% dan derajat deasetilisasi sebesar 96,95%. Spektra infrared menunjukan adanya gugus -NH, -OH, -CH dan –C=O yang sesuai dengan yang terdapat pada glukosamin. ABSTRACT: Glucosamine is a monomer from chitosan which can be obtained from small crab shell (P pelagicus) waste. Glucosamine is needed in the formation and repair of cartilage and other body tissues. This study aims to determine the characteristics of glucosamine from small crab shell waste. The process of isolating chitosan from small crab shells consisted of deproteination with 3N NaOH, demineralization with 1N HCl, and deacetylation with 50% NaOH. The chitosan obtained was analyzed its characteristics and degrees of deacetylation, then chitosan was chemically hydrolyzed with 20% HCl solution at room temperature for 4 hours. The resulting glucosamine is then calculated yield, loss on drying (LoD), solubility level and degree of deacetylation. The results showed that the yield of chitosan crab shells was 11.3%, white, odorless, 9.2% moisture content, 5.4% ash content, and 90.8% deacetylation rate. Glucosamine yield was 8.6%, with a Loss on Drying value of 1.3%, solubility of 72% and the degree of deacetylation of 96.95%. Infrared spectra show the presence of -NH, -OH, -CH and -C = O groups that match those found in glucosamine
Pengaruh Salinitas terhadap Pertumbuhan dan Aktivitas Antioksidan Dunaliella salina (Chlorophyceae: Dunaliellaceae) Setiasih, Intan Budi; Sabdono, Agus; Pramesti, Rini
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.008 KB) | DOI: 10.14710/jmr.v9i2.27028

Abstract

ABSTRAK: Dunaliella salina merupakan salah satu jenis mikroalgae hijau yang mengandung berbagai senyawa bioaktif termasuk senyawa antioksidan untuk melawan radikal bebas. Pertumbuhan mikroalgae dipengaruhi oleh lingkungan salah satunya adalah salinitas. Penelitian ini bertujuan mengetahui salinitas optimal untuk pertumbuhan dan aktivitas antioksidan D. salina berdasarkan nilai persen inhibisi. Penelitian ini dilaksanakan di Laboratorium Terpadu dan Laboratorium Biologi Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro, Semarang pada bulan Mei - Juli 2018. Metode penelitian yang digunakan adalah eksperimental laboratoris. D. salina dikultur pada lima salinitas yang berbeda yaitu 20, 25, 30, 35 dan 40 ppt.  Pengamatan dilakukan selama 7x24 jam, dipanen dan diekstrak dengan pelarut etanol yang selanjutnya dianalisis aktivitas antioksidannya dengan metode 1,1-difenil-2-pikrilhidrazil (DPPH). Hasil penelitian menunjukkan pertumbuhan optimal terjadi pada salinitas 30 ppt, dan aktivitas antioksidan tertinggi dicapai pada salinitas 20 ppt (9,88±0,59) yang termasuk dalam kategori lemah.ABSTRACT: D. salina is a type of green microalgae that contains various bioactive compounds including antioxidant compounds to fight free radicals. Microalgal growth is influenced by the environmental conditions such as  salinity. This study aims were to determine the optimal salinity of growth and antioxidant activity in ethanol extract based on percent inhibition values. This research was conducted in the Integrated Laboratory and Biology Laboratory of the Faculty of Fisheries and Marine Sciences, Diponegoro University, Semarang in May - July 2018.The research method used was an experimental laboratory. D. salina was cultivated with five different salinities on 20,  25, 30, 35 and 40 o/oo. Observation was carried out for 7x24 hours, harvested and extracted with ethanol solvent and then analyzed its antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The results showed that optimal growth of D. salina was 30 o/oo  in salinity, and the highest antioxidant activity was 20 o/oo in salinity (9.88 ± 0.59) and included in the weak category.
Co-Authors A. B. Susanto A.B. Susanto A.B. Susanto A.B. Susanto AB Susanto AB. Susanto Adam Sojuangon Pasaribu Adi Santoso Agus Sabdono Ahmad Fadhil Muzaki Ahmad Saddam Habibi Akbar, Muhamad Rahadian Akmal Nasrulloh Alfi Satriadi Ali Djunaedi Ali Ridlo Ananta, Raditya Rizki Andiska, Prismabella Wilis Anisah Harahap Anjani, Devi Oktavia Antonius Budi Susanto Ardiawan Pandu Romenda Ardiawan Pandu Romenda Bambang Yulianto C. Wiencke Chrisna Adhi Suryono Dafit Ariyanto Dara Ramadhania Istiqomahani Delianis Pringgenies Desni Elfrida Samosir Dini, Puti Sukma Rahma Edi Wibowo Kushartono Ega Hagita Testi Eldita Amalia Elfonso Robby Sitorus Endang Supriyantini Errizal Machmud Putra Faishal Islami, Faishal Feri Setiawan G. O. Kirst Galih S. Adiguna Gandang Herdananto Nugroho Ghofari, Miftahul Akhyar Gunawan Widi Santosa Ihsan Mahfudh Ika Asri Desanti Intan Budi Setiasih Irwani Irwani Istiqomahani, Dara Ramadhania Jelita Rahma Hidayati Karla Lutfia Rahmadanti Koesoemadji Koesoemadji Lovensia Zukruff Albasit Mahfudh, Ihsan Marhaeny, Annisa Maya Puspita Meli Ayu Pangestika Miftahul Akhyar Ghofari Mohamad Mirza Mohamad Mirza Muhamad Ihsan Muhamad Rahadian Akbar Muhammad Abdul Zaky Muhammad Zainuddin Muhammad Zainuddin Nasrulloh, Akmal Nirwani Nirwani Nirwani Soenardjo Nursiana Suci Wulandari Panjaitan, Kristina Venza Pasaribu, Adam Sojuangon Pramastuti, Fransisca Ria Prayudha, Muhammad Rifky Prismabella Wilis Andiska Puti Sukma Rahma Dini Putra, Errizal Machmud Raden Ario Radhian Wikanarto Widodo Raditya Rizki Ananta Rozi Hamdani Rudhi Pribadi Satria Sakti Budi Leksono, Satria Sakti Budi Sebastian Margino Setia Devi Kurniasih, Setia Devi Setiasih, Intan Budi Siti Rudiyanti Sitorus, Elfonso Robby Sri Sedjati Subagiyo Subagiyo Subagiyo Subagiyo Subagiyo Sunaryo Sunaryo Suryono Suryono Susanto, AB. Tarida Tarida Tarida, Tarida Testi, Ega Hagita Titis Buana Tiurma S Triyanto Triyanto Wahid Agung Saputra Wahyu Bagio Leksono Wakhid Indra Kusuma Widodo, Radhian Wikanarto Wilis A Setyati Wilis Ari Setyati Wulandari, Nursiana Suci Yohanes Oktaviaris Yulandaris Marsa Zaky, Muhammad Abdul