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Effect of Salinities and Dietary Patterns toward Fullness of Gut and Gut Evacuation Time of the Newly Introduced Penaeus merguiensis Larvae Mostafa Imhmed Ighwerb; Johannes Hutabarat; Ervia Yudiati; Rudhi Pribadi; Widianignsih Widianingsih; Retno Hartati; Abidin Nur II; Arumning T. Fauziah; Jelita Rahma Hidayati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 26, No 2 (2021): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.26.2.71-78

Abstract

The gut capacity of shrimp larvae is minimal, and their digestion is often challenged by the inevitable fact that they tend to develop slowly during the zoea stage. Many studies approved that the digestive capacity of shrimp larvae could be improved by increasing the retention time of food in the intestine. Gut evacuation time and fullness of the gut are crucial parameters in assessing the growth of shrimp larvae, and the diet as well as  environmental conditions indeed influence the activity of these parameters. Although many species of shrimps have a wide salinity tolerance, more specific research on salinity and its relation to the type of diet is necessary to find the optimum condition supporting the growth of shrimp larvae. By employing Penaeus merguiensis larvae, this study evaluates the effect of three nominal salinities (28, 32, and 36 ppt) and types of diets (Diet A: 100% live feed; Diet B: 100% FRIPPAK; Diet C: a combination of Diet A and Diet C, 50 % each) toward the fullness of gut and gut evacuation time of the newly introduced Penaeus merguiensis larvae culture. The result showed that the longest gut evacuation time and the highest percentage of gut's fullness were found in all Zoea reared with Diet A at salinity 28 and 32 ppt; Zoea-1 at 28 ppt with Diet A; Zoea-2 at 32 ppt with Diet A; Zoea-3 at 32 ppt with Diet A. Longer gut evacuation time would have an impact on the higher percentage of the fullness of gut. The higher fullness of the gut also indicates that the larvae have the best capacity to produce energy and achieve optimum growth.
Oral Administration of Alginate Oligosaccharide from Padina sp. Enhances Tolerance of Oxygen Exposure Stress in Zebrafish (Danio rerio) Ervia Yudiati; Rustadi Rustadi; Fanny Iriany Ginzel; Jelita Rahma Hidayati; Mila Safitri Rizfa; Nuril Azhar; Muhammad Salauddin Ramadhan Djarod; Eny Heriyati; Rabia Alghazeer
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 25, No 1 (2020): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (551.591 KB) | DOI: 10.14710/ik.ijms.25.1.7-14

Abstract

Alginate is rich in bioactive compounds and has been known to act as a stimulator on the innate immune system. The objective of this study is to determine polysaccharide and oligosaccharide alginate yield, that percentage inhibition with a different type of extraction, to evaluate growth performance as well as immune response by oxygen stress tolerance. Thermal heating with oven laboratory at 140oC for 4.5 hr was done to breakdown the polysaccharide into oligosaccharide. The extraction was conducted by maceration, filtration, precipitation, and centrifugation. Factorial design with two factors was implemented to 260 Zebrafish and reared in thirteen aquariums (20 fish per aquarium) for 12 days. Zebrafish was fed at different dose (4.0g; 6.0g; 8.0g.kg-1) and different type of extraction [noEDTA/noKCl; KCl; EDTA and (EDTAandKCl)]. The evaluation of radical scavenging activity was done spectrophotometrically at 515 nm. Results showed that the highest alginate yield either polysaccharide or oligosaccharide was gained from KCL treatments, percentage inhibition (82.61%), growth performance as well as tolerance of stress (P<0.05). The best growth performance was reached in oligosaccharide supplementation at 6.0g.kg-1 treatment. It can be concluded that alginate oliogosaccharide produced by thermal heating enhanced the antioxidant activity, boost the fish’s immune system, proofed by better growth performance and more tolerant to the low oxygen stress.
Aktivitas Antioksidan Ekstrak Rumput Laut Padina sp. Dari Perairan Bandengan Jepara Dengan Metode Transfer Elektron Jelita Rahma Hidayati; Ali Ridlo; Rini Pramesti
Buletin Oseanografi Marina Vol 6, No 1 (2017): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (622.165 KB) | DOI: 10.14710/buloma.v6i1.15742

Abstract

Antioksidan merupakan senyawa yang dapat menghambat atau mencegah reaksi oksidasi. Antioksidan sintetik kurang aman bagi kesehatan karena bersifat karsinogen, sehingga diperlukan sumber antioksidan alami seperti Padina sp. yang mengandung senyawa bioaktif seperti fenolat, klorofil a, karotenoid dan β-karoten. Padina sp. diambil dari Perairan Bandengan Jepara dan dikeringkan dengan Solar Tunnel Dryer (STD) selama 3 hari. Sampel dimaserasi secara bertingkat dengan pelarut n-heksana, etil asetat dan metanol lalu diuapkan dengan rotary evaporator. Aktivitas antioksidan ditentukan dengan metode DPPH (1,1-diphenyl-2-picrylhidrazyl) sebagai radikal bebas. Nilai IC50digunakan untuk mengetahui aktivitas antioksidan pada sampel yang diinkubasi selama 30 menit dan diukur nilai absorbansinya pada panjang gelombang 514 nm. Kandungan total fenolat diuji menggunakan larutan Folin-Ciocalteu dengan asam galat sebagai standar yang diukur pada panjang gelombang 725 nm. Kandungan klorofil a diukur pada panjang gelombang 663 nm dan 645 nm sedangkan karotenoid diukur pada panjang gelombang 480 nm. Hasil penelitian menunjukkan nilai IC50  terbaik dicapai ekstrak etil asetat yaitu 137,02 ppm, diikuti ekstrak n-heksana 1234,41 ppm dan ekstrak metanol 1554,45 ppm. Kandungan total fenolat tertinggi dicapai pada ekstrak etil asetat yaitu 12,09 mg GAE/g sampel, diikuti ekstrak n-heksana 9,32 mg GAE/g sampel dan ekstrak metanol 0,22 mg GAE/g sampel. Kandungan klorofil a tertinggi dicapai ekstrak etil asetat yaitu 2,67 mg/g sampel , diikuti ekstrak metanol 0,39 mg/g sampel dan ekstrak n-heksana 0,30 mg /g sampel. sampel). Kandungan karotenoid tertinggi dicapai ekstrak etil asetat yaitu 5,37 µ mol/g sampel, diikuti ekstrak n-heksana 1,85 µ mol/g sampel dan ekstrak metanol 0,53 µ mol/g sampel. Ekstrak etil asetat memiliki aktivitas antioksidan kategori sedang dan ekstrak metanol serta n-heksana memiliki aktivitas antioksidan kategori sangat lemah. Antioxidant are the compounds capable to inhibit and prevent oxidation reaction. Synthetic antioxidant less safe for health because it is a carsinogen, so required source of natural antioxidant as Padina sp. that contains bioactive compounds as fenolat, chlorophyll a, carotenoid and β-caroten. Padina sp was taken from Bandengan water Jepara and dried with Solar Tunnel Dryer (STD) during 3 days. Macerated sample were stratified with n-heksana, ethyl acetate and methanol then evaporated with a rotary evaporator. The antioxidant activity was determined using a DPPH solution (1,1-diphenyl-2-picrylhidrazyl) as free radicals. IC50 value used to determine the antioxidant activity of sample were incubated for 30 minutes and measured at wavelength 514 nm. Total phenolic content tested by the Folin-Ciocalteu solution with gallic acid as standard and measured at a wavelength of 725 nm, while the chlorophylls a content were measured at a wavelength of 663 nm and 645 nm and carotenoids were measured at a wavelength of 480 nm. The results showed best IC50 is achieved by ethyl acetat extract 137,02 ppm, followed by n-heksana extract 1234,41 ppm and methanol extract 1554,45 ppm.The highest total phenolic content is achieved by ethyl acetat extract 12,09 mg GAE/g sample, followed by n-heksana extract 9,32 mg GAE/g sample and methanol extract 0,22 mg GAE/g sample. The highest chlorophyll a content is achieved by ethyl acetat extract 2,67 mg/g sample, followed by methanol extract 0,39 mg/g sample and n-heksana extract 0,30 mg/g sample. The highest carotenoids content is achieved by ethyl acetat extract 5,37 μmol/g sample, followed by n-heksana extract 1,85 μmol/g sample and  methanol extract 0,53 μmol/g sample. Eethyl acetate extract categorized as medium antioxidant activity and methanol and n-heksana extracts categorized as very weak antioxidant activity.
Antioxidant Activities, Total Phenolic Compound And Pigment Contents of Tropical Sargassum sp. Extract, Macerated In Different Solvents Polarity Jelita Rahma Hidayati; Ervia Yudiati; Delianis Pringgenies; Zaenal Arifin; Diah Tri OktaviyantI
Jurnal Kelautan Tropis Vol 22, No 1 (2019): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.41 KB) | DOI: 10.14710/jkt.v22i1.4404

Abstract

Exposure of sunlight lead tropical Sargassum sp. to maintain their growth and moreover to bring up their secondary metabollite for life struggling. Sargassum sp. has bioactive compounds that has a potential antioxidant acitivity such as phenolic compounds as well as chlorophyll and carotenoids. This research was conducted to determine antioxidant activities, phenolic compound and pigmens of Sargassum sp. with different solvent that have different polarities. Sample was macerate with n-heksana, ethyl acetate, methanol and aquadest. All the parameters were done spectrophotometrically. IC50 was used to determine the antioxidant activity by antiradical scavenging activity using DPPH (515 nm). Total phenolic compound were tested by Folin-Ciocalteu solution  and used gallic acid as standard (725 nm). The chlorophylls a content were measured at wavelength 662 nm and 645 nm and carotenoids were measured at wavelength 470 nm. The results showed best IC50 is achieved by aquadest extract (72.95 ±0.22 ppm). The highest Total phenolic compound is achieved by ethyl acetat extract (120.29 ±0,404 mg GAE/g sample). The highest chlorophyll a content is achieved by ethyl acetat extract (18.23 ± 0,049 mg/g sample) and the highest carotenoid content is achieved by ethyl acetat extract extract (60.65 ±0,008  µmol/g sample). It can be concluded that aquadest extract can be categorized as the strong antioxidant and antiradical activity, ethyl acetat as a medium antioxidant activity. The simple and save methods of aquadest extract promising that Sargassum  sp. frorn Indonesia is a good candidate compoud for nutraceutical and cosmeceutical approach.  
Antioxidant activity of Alginate Oligosaccharides (AOS) from Sargassum sp. for Improving the Cutaneous Wound Enclosure in Zebrafish (Danio rerio) Jelita Rahma Hidayati; Ervia Yudiati; Delianis Pringgenies; Zaneb M. Ben Mansur; Fadhliyah Idris
Jurnal Kelautan Tropis Vol 24, No 3 (2021): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v24i3.12362

Abstract

Sargassum sp. classified as brown seaweed which is known as an alginophyte (alginate producer). Alginate has undergone a depolymerization process called alginate oligosaccharides (AOS) and has been shown to have antioxidant activities to increase wound tissue recovery. This study aimed to determine the antioxidant activity of Alginate oligosaccharides (AOS) and their ability to improve the cutaneous wound enclosure in Zebrafish (Danio rerio). The IC50 value was used to calculate the ability of extract to inhibit free radicals using DPPH (516 nm). Zebrafish were immersed 12 hours before the injury and shortly after injury with a two factorial design, i.e., alginate concentration and immersion time. Zebrafish were immersed for 1 hour, 3 hours, and 5 hours with serial concentration of 200 ppm, 400 ppm, and 600 ppm, respectively. Morphological observations were carried out at the the first day, fourth day, tenth day, and twenty-first-day post wounding. The results of this study showed that alginate from Sargassum sp. has a yield of 40.5 ± 1.125% with a purity level of 89.95%. Based on antioxidant activity, alginate is categorized as moderate (178,377 ppm) and evidently has the ability to increase wound recovery compared to control. It has indicated by the formation of the wound enclosure.
Sosialisasi penyemaian bibit dari buah Enhalus acoroides untuk menjaga keberlanjutan ekosistem Lamun di Desa Pengudang Pulau Bintan Ita Karlina; Aditya Hikmat Nugraha; Dony Apdillah; Jelita Rahma Hidayati; Esty Kurniawati
Unri Conference Series: Community Engagement Vol 3 (2021): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.3.148-155

Abstract

Seagrass ecosystems contribute to food security, climate change mitigation, and biodiversity, yet they are vulnerable to changes in the environment. Environmental factors, both natural and human-caused, are the primary cause of the loss in the area of seagrass ecosystems. Transplanting or sowing seagrass is one method for addressing this issue. The goal of this project is to maintain the seagrass environment and give coastal people with knowledge so that they may contribute to the conservation of coastal habitats, particularly seagrass ecosystems, in Pengudang Village, Bintan. The seeding process starts with a search for seagrass in areas with a dense seagrass habitat. Enhalus acoroides was chosen as the seeding object in this investigation. The findings revealed that 78% of respondents were highly aware of the existence of seagrass ecosystems (perception), 28% were very aware of the function and existence of seagrass ecosystems (understanding), and 86% significantly participated in seagrass ecosystem conservation (participation).
Asosiasi Siput Gonggong (Strombus sp.) pada Ekosistem Lamun Di Pesisir Timur Pulau Bintan Nurul Hati; Ita Karlina; Rika Angraeni; Aditya Hikmat Nugraha; Fadhliyah Idris; Jelita Rahma Hidayati
Jurnal Kelautan Tropis Vol 25, No 2 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i2.13414

Abstract

The east coast of Bintan Island is an area that has a wide distribution of seagrass with good conditions for the association of Strombus sp in the seagrass ecosystem. This research aims to identify seagrass coverage and abundance of golden snails (Strombus sp) and study the association of golden snails (Strombus sp.) in seagrass ecosystems on the east coast of Bintan Island. This research was conducted on the East Coast of Bintan Island from June until July 2021 (Teluk Bakau Village, Malang Rapat Village and Berakit Village). The tools and materials used in this research were GPS, 1x1 m quadrant transect, roller meter, digital scales, calliper, multitester, hand refractometer, pH meter, pipe (20cm), sieve net, stationery and aluminium foil. The sampling method used three transects with a distance of 100 m towards the sea with a distance between transects of 50 m, with a 1 x 1 m quadrant transect beginning from point 0 until 100 m. Data analysis used in this research were seagrass coverage, Strombus sp. abundance and PCA (Principal Component Analysis) analysis which relates the abundance of barking snails, seagrass cover, water physicochemical parameters and substrate. Strombus sp. found are Strombus urceus, Strombus canarium and Strombus turturella. The Strombus urceus type has a high 4,3 ind/m2 abundance value compared to other types of Strombus sp. at all stations. Types of Strombus canarium and Strombus turturella were mostly found in low seagrass cover and associated with seagrass species Halophila, Thalassia hemprichii and Halodule uninervis, while Strombus urceus species were mostly found in medium and dense seagrass cover and positively associated with Enhalus acoroides, Thallasia hemprichi, Cymodocea rotundata.    Pesisir Timur Pulau Bintan merupakan wilayah yang memiliki sebaran lamun yang luas dengan kondisi baik bagi asosiasi Strombus sp. pada ekosistem lamun. Tujuan dari penelitian ini untuk mengidentifikasi tutupan lamun dan kelimpahan siput gonggong (Strombus sp.) dan mempelajari asosiasi siput gonggong (Strombus sp.) pada ekosistem lamun di pesisir Timur Pulau Bintan. Penelitian ini dilakukan di Pesisir Timur Pulau Bintan pada bulan Mei hingga Juni 2021 (Desa Teluk Bakau, Desa Malang Rapat dan Desa Berakit). Alat dan bahan yang digunakan dalam penelitian ini adalah GPS, transek kuadran 1x1 m, rol meter, timbangan digital, jangka sorong, multitester, hand refracstometer, pH meter, pipa paralon (20cm), sieve net, alat tulis dan alumunium foil. Metode sampling menggunakan 3 transek dengan panjang 100 m ke arah laut dengan jarak antar transek yaitu 50 m, dengan transek kuadran 1 x 1 m dimulai dari titik 0 sampai 100 m. Analisis data yang digunakan dalam penelitian ini yaitu tutupan lamun, kelimpahan Strombus sp., serta analisis PCA (Principal Component Analysis) yang menghubungkan antar variabel kelimpahan siput gonggong, tutupan lamun, parameter fisika kimia perairan dan substrat. Jenis Strombus sp. yang dijumpai diantaranya Strombus urceus, Strombus canarium dan Strombus turturella. Jenis Strombus urceus memiliki nilai 4,3 ind/m2 kelimpahan yang tinggi dibandingkan dengan jenis Strombus sp. lain pada seluruh stasiun. Jenis Strombus canarium dan Strombus turturella banyak ditemukan pada tutupan lamun yang rendah serta berasosiasi dengan jenis lamun Halophila, Thalassia hemprichii dan Halodule uninervis, sedangkan jenis Strombus urceus banyak ditemukan pada tutupan lamun yang sedang dan padat serta berasosiasi positif dengan Enhalus acoroides, Thallasia hemprichi, Cymodocea rotundata.
Antioxidant Activity and Bioactive Compounds of Tropical Brown Algae Padina sp. from Bintan Island, Indonesia Jelita Rahma Hidayati; Muhammad Syaifudien Bahry; Ita Karlina; Ervia Yudiati
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.15562

Abstract

Macroalgae is one of the marine resources that have the ability as an antioxidant. Its ability is obtained from bioactive compounds produced through secondary metabolism. One type of macroalgae that has the potential as an antioxidant is Padina sp. This study aims to analyze the content of bioactive compounds in Padina sp and determine their antioxidant activity using DPPH as free radicals. Padina sp. was taken from the Bintan waters and shade-dried for three days. Bioactive compounds were analyzed through phytochemical screening to determine the content of flavonoid compounds, steroids, triterpenoids, saponins, and tannins. Determination of antioxidant activity begins with measuring the maximum wavelength of DPPH at 400-800 nm and determining the incubation time of the sample and DPPH. Antioxidant activity was determined based on the value of Inhibition concentration (IC50) at a wavelength of 515.5 nm; total phenolic content was determined using gallic acid standard (725 nm); total flavonoid content was determined using quercetin standard (415 nm), chlorophyll a and carotenoids were selected to determine pigment content on the sample. The results showed that Padina sp. contains flavonoid compounds, steroids, and tannins. Extract of Padina sp. has a total phenolic content of 46.02 mg/GAE g; total flavonoid content of 35.36 mg/QE g; chlorophyll content of 9.18 mg/g; and carotenoid content of 26.46 µmol/g. Methanol extract of Padina sp. has an IC50 value of 92.17 ppm and is classified as a strong antioxidant.
Artemia with Synbiotics Enrichment Improves Resistance Against Vibrio parahaemolyticus AHPND of Litopenaeus vannamei Larvae Ervia Yudiati; Zaenal Arifin; Adi Santoso; Jelita Rahma Hidayati; Rabia Alghazeer; Nuril Azhar
Buletin Oseanografi Marina Vol 12, No 3 (2023): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v12i3.52523

Abstract

Shrimp, a high-protein food commodity, is one of the world's fastest-growing food-producing sectors. The present research aimed to find out the survival and growth of Litopenaues vannamei Post Larva (PL) and the resistance against VpAHND and stress salinity. 1500 PL were reared to two sets of experiments at the density of 50 PL. L-1. The first set is purposed to determine the PL growth, resistance to Vibrio paramaemolyticus AHPND challenge and stress salinity. The second set is purposed to determine the survival rate. A Completely randomized design (CRD) with five treatments and three replications was conducted. The treatments are Artemia enrichment with different Alginate doses and probiotics (400, 600, 800 ppm Alg+pro), probiotics (Pro), and control without any synbiotics addition. PL was reared in 14 days. The survival rate, and weight gain were calculated. At the end of the experiment, 10 PL was challenged against VpAHPND at 1 x 107 CFU mL-1by immersion methods. Twenty PL was exposed to stress salinity and shocked from 25 ppt to 0 ppt. The best survival rate ((78±2%), and tolerance to osmotic stress was reached at PL fed on a combination of alginate and FNCC-002 Lactobacillus bulgaricus probiotics (p<0.05). PL fed on Artemia enriched probiotics reached the highest resistance to severe VpAHNPD. The weight gain among treatments is similar. It can be concluded that synbiotics of alginate as prebiotics and FNCC-004 probiotics work synergically and this might be interrelated with immune response.  
Estimasi Cadangan Karbon Pada Ekosistem Mangrove Di Desa Pengudang Kecamatan Teluk Sebong Kabupaten Bintan Diah Alviana; Rika Anggraini; Jelita Rahma Hidayati; Ita Karlina; Febrianti Lestari; Dony Apdillah; Agung Dhamar Syakti; Dermawati Sihite
Jurnal Kelautan Tropis Vol 26, No 3 (2023): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v26i3.18326

Abstract

Mangrove have a role in the absorption and storage of carbon from the atmosphere, which is stored in the form of biomass and sediment, so that mangroves have enormous potential in mitigation global climate change because they can reduce greenhouse gas emission is carbon. Mangrove store carbon in carbon pools including above ground biomass, below ground biomass, litter or dead wood, and soil. The purpose of this study was to calculate the value of carbon stock and carbon sequestration in mangroves in Pengudang Villages, Teluk Sebong District, Bintan Regency. This research was conducted in September – October 2022, the research method used purposive sampling non-destructive method with allometric model and sediment sampling was carried out at 30 cm intervals until the discovery of humus soil. The result of biomass calculations were then converted into carbon stock values and mangrove carbon sequestration. Data analysis was carried out by calculating biomass, carbon stock value and carbon sequestration in mangroves in Pengudang Villages, Teluk Sebong District, Bintan Regency. The result showed the total biomass value was 498,77 tons/ha, the carbon stock value was 1203,27 tonsC/ha, while the carbon sequestration value was 4412,05 tonsC/ha.  Mangrove berperan dalam penyerapan dan penyimpanan karbon dari atmosfer yang disimpan dalam bentuk biomassa dan sedimen, sehingga mangrove memiliki potensi yang sangat besar dalam mitigasi perubahan iklim global karena dapat mengurangi emisi gas rumah kaca yaitu karbon. Mangrove menyimpan karbon dalam kantong karbon diantaranya adalah biomassa bagian atas, biomassa bagian bawah, serasah atau kayu mati dan tanah.  Tujuan penelitian ini untuk menghitung nilai stok karbon dan sekuestrasi karbon pada mangrove di Desa Pengudang Kecamatan Teluk Sebong Kabupaten Bintan. Penelitian ini dilakukan pada bulan September – Oktober tahun 2022, metode penelitian yang digunakan adalah purposive sampling metode non-destruktif dengan model alometrik dan dilakukan pengambilan sampel sedimen per-interval 30cm hingga ditemukannya tanah humus. Hasil perhitungan biomassa kemudian dilakukan konversi nilai stok karbon dan sekuestrasi karbon mangrove. Analisis data dilakukan dengan menghitung biomassa, nilai stok karbon dan sekuestrasi karbon pada mangrove di Desa Pengudang Kecamatan Teluk Sebong Kabupaten Bintan. Hasil penelitian menunjukkan nilai total biomassa 498,77 ton/ha nilai stok karbon adalah 1203,27 tonC/ha sedangkan nilai sekuestrasi karbon adalah 4412,05 tonC/ha.