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Journal : Jurnal Analis Medika Biosains (JAMBS)

Analisis Konsentrasi Letal Dan Waktu Letal Isolat Jamur Aspergillus Niger Terhadap Nyamuk Aedes Aegypti Ni Diah Kadek Sinta Cahyani; Ida Bagus Rai Wiadnya; Ari Khusuma; I Wayan Getas
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 2 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i2.264

Abstract

Latar Belakang: Nyamuk Aedes aegypti merupakan penyebab utama penyakit DBD, kasus DBD di dunia pada tahun 2015 sebanyak 3,2 juta kasus. Di Indonesia pada tahun 2018 terdapat 65.602 kasus. Upaya pengendalian terhadap vektor DBD telah banyak dilakukan baik dengan menggunakan insektisida kimia dan alami salah satunya dengan jamur Aspergillus niger sebagai fungi entomopatogen. Tujuan Penelitian: Mengetahui konsentrasi letal dan waktu letal dari isolat jamur Aspergillus niger terhadap nyamuk Aedes aegypti.  Metode Penelitian: Penelitian ini merupakan penelitian true eksperimental dengan rancangan acak lengkap. Perlakuan dengan 3 konsentrasi isolat jamur Aspergillus niger yaitu 10-5, 10-6, 10-7 spora/ml dengan 6 replikasi. Penelitian ini menggunakan obat nyamuk semprot sebagai kontrol positif. Data diperoleh dianalisa secara statistik menggunakan analisis probit. Hasil Penelitian: Konsentrasi isolat jamur Aspergillus niger yang efektif terhadap nyamuk Aedes aegypti adalah konsentrasi 10-7. Hasil analisis probit uji patogenitas isolat jamur Aspergillus niger terhadap nyamuk Aedes aegypti dengan tingkat kepercayaan 95% didapatkan LC50, LC70, LC90 adalah 6,1 x 107 spora/ml, 8,5 x 107 spora/ml, 1,1 x 108 spora/ml dan diketahui LT50, LT70, LT90 berturut-turut adalah 1,919 jam, 1,510 jam, 1,184 jam. Kesimpulan: Konsentrasi isolat jamur Aspergillus niger secara signifikan memberikan pengaruh terhadap kematian nyamuk Aedes aegypti.
Analisis Jumlah dan Jenis Bakteri Coccus Gram Positif pada Sisa Sampel Darah di Spuit Sofiyan Dwi Arfi; Erna Kristinawati; Ari Khusuma
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 2 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i2.270

Abstract

.Background: One of the problems in the field of health services that can increase the mortality rate is nosocomial infection. Waste containing pathogenic bacteria can serve as a medium for the spread of disease for health facility workers, sufferers and the public. One of the medical devices that have a high risk factor as an intermediary for infection is a syringe. Bacterial contamination of the syringe after use cannot be ignored. Therefore, further research is needed on waste in health facilities to determine infectious diseases caused by residual blood samples in the syringe. Research Objectives: To determine the presence of gram-positive coccus bacteria in the remaining blood sample in the syringe. Methods: This study used a descriptive observational method with a cross sectional approach to time. The sample size in this study was 14 samples with a sampling technique that is total sampling. Results: It was found that Staphylococcus aureus and Staphylococcus cohnii were found each of the 14 samples. Conclusion: There are gram-positive coccus bacteria, namely Staphylococcus cohnii and Staphylococcus aureus. 
Utilization Of Kuku Pancar (Lawsonia Inermis Lin) Leaves as a Substitute For Eosin Dye in The Examination of Soil Transmitted Helminth (STH) Eggs Lala Deswinta Yusari; Lalu Srigede; Thomas Tandi Manu; Ari Khusuma
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.356

Abstract

Nail surfacing leaves (Lawsonia inermis lin) are natural materials commonly used as dyes, because of the content of chemical compounds in them such as lawsone, flavonoids, tannins and anthocyanins, therefore nail surfacing leaves can be used as an alternative dye to replace 2% Eosin in STH worm egg examination, because it has the same properties as 2% Eosin and can produce an orange yellow color after the extraction process. The research objective is to determine the results of nail surfacing leaves (Lawsonia inermis lin) can be used as an alternative substance to replace Eosin in the examination of STH (soil transmitted helminth) worm eggs. The methods of this research is using is experimental, where this study looks at the clarity of soil transmitted helminth (STH) worm eggs on preparations, using coloring from the results of soaking the leaves of the nail surf as a substitute for Eosin. The results of the research is that the coloring results obtained in each variation of immersion time are good. The conclusion of this research is that the staining of worm eggs using the results of immersion of nail surfacing leaves for 12 hours, 24 hours, 36 hours, and 48 hours can be used as an alternative dye in the examination of STH worm eggs.