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Potensi Pasta Gigi Minyak Atsiri Temulawak (Curcuma xanthorriza Roxb) dalam Menghambat Pembentukan Plak dan Gingivitis pada Tikus yang Diinduksi Phorpyromonas gingivalis Yumnaina Nurhadi; Pujiana Endah Lestari; Peni Pujiastuti
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 19 No 2 (2022)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v19i2.34739

Abstract

Gingivitis is an inflammatory disease in gingiva without any loss of attachment caused by plaque. Gingivitis is reversible, if not treated it can develop into periodontitis. Gingivitis-causing bacteria include Phorpyromonas gingivalis, acting as secondary bacteria in plaque formation at the secondary colonization stage by attaching themselves to monolayers that have been formed by primary bacteria. Herbal use in Indonesia is still a trend until now with the slogan back to nature, one of the easy to find and useful is essential oil from curcuma rhizomes containing xanthorrizol and curcumin. They are antibacterial that can inhibit the formation of plaques that cause gingivitis. The purpose was to analyze the potential of curcuma essential oil toothpaste in inhibiting the formation of plaque and gingivitis. 27 wistar mice were divided into 9 groups, namely negative control group, positive control, treatment applied curcuma essential oil toothpaste concentration of 0.5%, 1%, 2%, 4%, 8%, 16% and 32%. Each mouse induced P. gingivalis 0.02 ml on molar sulkus one and two, 24 hours later performed tooth brushing with toothpaste according to the group, the next 24 hours were observed in day 3, 5 and 7 using plaque index according to Park and Katz and gingiva index according to Lee. Treatment and observation are carried out 3 repetitions. The results of the plaque index score and gingiva index in the treatment group were fewer than the negative controls. Curcuma essential oil toothpaste can inhibit the formation of plaque and gingivitis by decreasing the plaque index and gingiva index in P. gingivalis- induced wistar mice.
Management of Localized Gingival Overgrowth in Patient under Fixed Orthodontic Treatment: A Case Report Neira N. Sakinah; Yuliana M. D. Arina; Depi Praharani; Peni Pujiastuti; Desi S. Sari; Melok A. Wahyukundari
e-GiGi Vol. 10 No. 2 (2022): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v10i2.40683

Abstract

Abstract: Recently, the need for orthodontic treatment has increased along with the increasing need and public awareness of the importance of dental and oral health. During treatment, problems sometimes occur with periodontal tissue. Orthodontic devices that bond to the tooth surface can cause difficulties in controlling plaque, especially in marginal areas which can cause inflammation and gingival overgrowth. Too high tensile strength and weak tissue capacity are also the factors that can cause gingival overgrowth. This case report described the management of gingival growth in a patient under fixed orthodontic treatment. We reported an 18-year-old female patient with a good systemic condition that had gingival overgrowth localized on the anterior part of mandibula. The surgical intervention was performed by using conventional surgical methods. Its goal was to remove the gingival pocket, therefore, the plaque could be easily controlled. The use of a scalpel was considered very effective for eliminating excessive gingiva. Two weeks after surgical treatment, the gum resulted in good repair. The continuity of orthodontic treatment could be achieved properly after 3-month surgical treatment. Instructions and motivation to maintain oral hygiene were important to avoid recurrence. In addition, multidisciplinary dentistry between orthodontist and periodontist was needed to achieve a better result. Keywords: gingival overgrowth; gingivectomy; orthodontic appliance
The potential of toothpaste containing Robusta coffee bean extract in reducing gingival inflammation and dental plaque formation Peni Pujiastuti; Neira Najatus Sakinah; Yuliana Mahdiyah Da’at Arina; Melok Aris Wahyukundari; Depi Praharani; Desi Sandra Sari
Dental Journal Vol. 56 No. 2 (2023): June 2023
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i2.p109-114

Abstract

Background: The prevention of gingivitis using chemicals containing antibiotics and chlorhexidine can disrupt the balance of the oral microbiota and have side effects in long-term use. A recent development in the prevention of gingivitis is the use of natural ingredients. Coffee is a natural ingredient that compounds several antibacterial and anti-inflammation properties. Purpose: The study aimed to determine the potential of toothpaste containing Robusta coffee bean extract in reducing gingival inflammation and inhibiting the formation of dental plaque. Methods: Twenty male Rattus norvegicus were divided into four groups, namely the control group and treatment groups (TG) TG25%, TG50%, and TG75%. All groups were fitted with ligature wire on the first left molar to accumulate dental plaque. After the fourth day, the ligature wire was removed, and the TG25%, TG50%, and TG75% groups were brushed once a day using toothpaste containing various concentrations of Robusta coffee extract, while the control group was brushed without using toothpaste. Plaque index, gingival index, and interleukin-1 (IL-1) expression were observed on the fifth day. The data was statistically tested using a one-way analysis of variance and post hoc least significant difference. Results: The statistical test showed that the TG75% group had the lowest value of plaque, gingival index, and IL-1 expression, while the control group had the highest (p < 0.05). Conclusion: Robusta coffee bean extract toothpaste has the potential to reduce gingival inflammation and dental plaque formation in a rat with gingivitis. The most effective concentration of Robusta coffee bean extract toothpaste in reducing gingival inflammation and dental plaque formation was 75%.
NUMBER OF BLACK-PIGMENTED ANAEROBIC BACTERIA CONTAINED IN THE GINGIVAL CREVICULAR FLUID (GCF) OF PATIENTS SUFFERING FROM THE CHRONIC PERIODONTITIS AND AGGRESSIVE PERIODONTITIS Depi Praharani; Peni Pujiastuti; Melok Aris Wahyukundari; Yuliana Mahdiyah Daat Arina; Desi Sandra Sari
Odonto : Dental Journal Vol 8, No 1 (2021): July 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (574.238 KB) | DOI: 10.30659/odj.8.1.101-107

Abstract

THE EFFECTIVENESS OF USING TOOTHPASTE CONTAINING ROBUSTA COFFEE BEAN EXTRACT IN INHIBITING THE FORMATION OF DENTAL PLAQUE Rina Nanda Prasasti; Desi Sandra Sari; Peni Pujiastuti
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.412 KB) | DOI: 10.30659/odj.9.1.12-20

Abstract

Background: Periodontal disease is caused by the interaction between pathogenic bacteria and plaque, without the accumulation of plaque, periodontal disease will not appear. Plaque will be visible one to two days if no oral cleaning steps are taken. The combination of antimicrobial therapy such as antibiotics and mouthwash as well as mechanical therapy such as scaling and root plain gives more effective results. However, if done in the long term it will cause resistance. To overcome this, alternative materials are needed that are used to inhibit bacterial growth, one of which is robusta coffee beans. The content of robusta coffee bean extract contains phenolic and flavonoid compounds that function as antibacterials that work by disrupting the function of the cytoplasmic and cytoplasmic membranes of bacteria.Method: In this study, 6 groups were used, namely KN, K-, K+, KP 25%, KP 50%, and KP 75%. The gingivitis model was made with wire using ligation technique for 3 days, then toothpaste was applied, brushing process, and disclosing agent was given, and the plaque index calculation was PHP (Personal Hygiene Performance). The data obtained were then analyzed using the Kruskall-Wallis test followed by the Mann-Whitney test.Result: The Robusta coffee bean extract toothpaste group at a concentration of 75% experienced a significant reduction in plaque compared to the negative control group and the 25% treatment group (p<0.05), but there were no significant differences or had the same ability when compared to the experimental group. 50% treatment group, normal control group and positive control group.Conclusion: The 75% concentration of Robusta coffee bean extract found in toothpaste is proven to be effective in inhibiting the formation of dental plaque.
KADAR KALSIUM (Ca) DALAM CAIRAN KREVIKULAR GINGIVA PADA PENDERITA PERIODONTITIS KRONIS Ita Kurniawati; Peni Pujiastuti; Agustin Wulan Suci Dharmayanti
Odonto : Dental Journal Vol 2, No 2 (2015): December 2015
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (87.087 KB) | DOI: 10.30659/odj.2.2.11-16

Abstract

Background: Chronic periodontitis occurred mostly in productive ages. Chronic periodontitis was started by adherence and accumulation of plaque bacteria that caused vascular enhancement, PMN infiltration, and periodontal tissue destruction. This process caused bone calcium solubitily which went trough blood stream and excreted to gungiva sulci. Calcium in periodontal tissue could be found both in extracellular and intracellular matrix.Purpose: Purpose of this study was to know calcium level in gingival crevicular fluid of chronic periodontitis and compare calcium level in chronic periodontitis and gingivitis.Method: This study was analytic observational with cross sectional approach and got admission from ethic comission of Dental Faculty, Gadjah Mada University. The subjects were patients who came to dental hospital of Jember University. The subjects had to full fill informed consent. The subjects were selected according inclusion and exclusion criteria. Subjects  complying inclusion criteria were divided into gingivitis group (PI score 0 - 0.7) and chronic periodontitis (PI score 1 - 8.0). Gingival crevicular fluid samples were collected from teeth which got gingivitis and chronic periodontitis, GCF was absorbed using paperpoint and stored inside eppendorf tube. Paperpoint was added with 50 µL 0.02 M PBS pH 7.4 and 100 µL distilled water. Calcium level was measured by Atomic Absorption Spectrophotometer (AAS). Result: The result showed there was calcium in gingival crevicular fluid of chronic periodontitis. Mean of calcium level of chronic periodontitis was higher than  gingivitis.Conclusion: This study concluded there was calcium in gingival crevicular fluid of chronic periodontitis and calcium level of chronic periodontitis was higher than gingivitis.
Daya Hambat Pasta Gigi yang Mengandung Ekstrak Biji Kopi Robusta (Coffea canephora) Terhadap Pertumbuhan Bakteri Aggregatibacter actinomycetemcomitans Secara In Vitro Farahdila, Nihla Adinda; Pujiastuti, Peni; Sari, Desi Sandra
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 21 No 1 (2024)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v21i1.47335

Abstract

Periodontitis is caused by bacteria in plaque, namely Aggregatibacter actinomycetemcomitans. Prevention of periodontal disease can be done by controlling plaque, namely brushing teeth using toothpaste to remove plaque and prevent plaque maturation. Alternative ingredients (herbs) that can be used to make toothpaste are robusta coffee beans which contain caffeine, flavonoids, trigonelin, and chlorogenic acid as antibacterial. The purpose of this study was to analyze the inhibition of toothpaste containing robusta coffee bean extract at concentrations of 6.25%, 12.5%, 25%, and 50% against the growth of A. actinomycetemcomitans. This type of research is experimental laboratory with posttest only control group design. This study was divided into 6 groups, namely positive control (Arabica coffee toothpaste), negative control (placebo paste), Robusta coffee bean extract toothpaste 6.25%, 12.5%, 25%, and 50%. Inhibition test using well diffusion method by making holes in the media. The diameter of the inhibition zone was measured using a caliper. The results showed that the control (-) group and the 6.25% group had no inhibitory power against the growth of A. actinomycetemcomitans. The robusta coffee bean extract toothpaste at concentrations of the 50% group (18.69 mm) had the greatest inhibition, but K (+) had greater inhibition than the 50% (22.89 mm) group. The conclusion of this study is that the robusta coffee bean extract toothpaste at concentrations of 12.5%, 25%, and 50% had inhibitory power against the growth of A. actinomycetemcomitans, but the greatest inhibitory power was at a concentration of 50%.
POTENTIALLY OF PURPLE LEAVES TO INCREASE OSTEOBLASTAT ALVEOLAR BONE RAT INDUCED PORPHYROMONAS GINGIVALIS Dyasti, Pramita Wahyu; Kurniawati, Atik; Pujiastuti, Peni
Journal of Vocational Health Studies Vol. 4 No. 3 (2021): March 2021 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V4.I3.2021.114-118

Abstract

Background: Periodontal disease are common dental and oral health problems in the community. Porphyromonas gingivalis (Pg) is one of the main causes of the periodontal disease. The alveolar bone resorption could be as severity indicator of the disease, so that need a material that can help a process of bone remineralization. Osteoblasts are bone-forming cells that are responsible for the mineralization of the bone matrix. Purple leaf is one of the thirteen commodities developed by DitjenPOM as a superior medicinal plant. Purpose: To determine the effect of 2,5%, 5% and 10% purple leaf extract (EDU) of osteoblasts cells in wistar rats induced by Pg. Method: Thirty male wistar rats divided into 5 groups, namely N (without treatment), K (Pg), P (Pg+EDU 2,5%), P2 (Pg+ EDU 5%), P3 (Pg+EDU 10%). EDU administration once a day for 7 days. The mice were decaputated on the 7 th day and the left mandible was taken to make preparations followed by HE staining, observation and calculation of the number of osteoblast cells in 3 different fields. The results of the observations (data) were added up, averaged and analyzed by One Way ANOVA. Result: The group induced by Pg and EDU had a higher average number of osteoblasts than the K and N group. Conclusion: Purple leaf extract (EDU) can increase osteoblasts cells in the alveolar bone of male wistar rats induced by Pg with the highest number of osteoblasts in the 10% concentration.
IDENTIFICATION OF ESCHERICHIA COLI AND ORGANIC SUBSTANCES BASED ON THE DISTANCE FROM THE DUG WELL TO THE SEP-TIC TANK Brilyan Nissa, Gendis; Pujiastuti, Peni
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 11 No. 1 (2024): Biolink Agustus
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v11i1.11485

Abstract

As many as 70% of drinking water sources in Indonesia are contaminated with feces which can cause the spread of diarrheal diseases. There were 1,177 cases of diarrhea spread across Colomadu District. In Pucung Village, Colomadu District, complaints about well water being colored, smelly, tasteful, slimy, and people often experiencing diarrhea, especially those located less than 10 m from the septic tank. In accordance with Minister of Health Regulation Number 32 of 2017 concerning Water Hygiene, the E. coli content is 0 CFU/100 ml and the maximum organic material content is 10 mg/l. The E. coli analysis method uses Total Plate Count (TPC) on agar plates according to SNI 2897:2008. Research needs to be carried out to ensure the quality of well water regarding the content of E. coli and organic substances. Determination of organic substance levels was carried out using the permanganometric titration method according to SNI 06-6989.22-2004. This research uses the TPC method to determine water quality and is included in qualitative research which focuses on case studies. Based on the research, the results showed that the values ​​of organic substances and E. coli in sample I were 17.20 mg/l and 1.6 x 109 CFU/100 ml, sample II was 11.88 mg/l and 5.0 x 105 CFU / 100 ml, while sample III was 6.88 mg/l and 2.3 x 104 CFU/100 ml. So, the quality of the well water in the Pucung Village studied did not meet the requirements of Minister of Health Regulation Number 32 of 2017.  
Pengabdian Masyarakat Internasional Penguatan Ecowisata Potensi Desa Segorogunung, Kab. Karanganyar Setyawan, Didik; Herawati, Dewi Astuti; Siddiq, Faiz Rahman; Hariyanti, Widi; Pujiastuti, Peni; Suseno, Suseno; Wibowo, Yari Mukti; Anita Indrasari; Kresnadipayana, Dian; Rahmadina, Nadia; Muftiyanto, R. Taufiq Nur
Journal Of Human And Education (JAHE) Vol. 5 No. 1 (2025): Journal of Human And Education (JAHE)
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jh.v5i1.2221

Abstract

Potensi Desa Segorogunung ini baik hasil pertanian maupun wisata yang berbasis alam diharapkan memberikan kontribusi pada pendapatan desa maupun masyarakatnya. Wisata yang mengembangkan ekowisata pada hakikatnya merupakan bentuk wisata yang bertanggungjawab terhadap keberlanjutan area wisata yang alami, memberi manfaat ekonomi kepada masyarakat sekitar dan mempertahankan kelestarian budava bagi masyarakat setempat. Potensi ekowisata Desa Segorogunung diperlukan integrasi yang dapat memberikan keuntungan kepada masyarakat. Terdapat 3 area kegiatan pengabdian masyarakat yang dilakukan, yaitu pengembangan potensi kebun teh untuk wisata, pemanfaatan pemasaran digital dalam menyampaikan informasi dan kegiatan di Bumi Perkemahan Desa Segorogunung, dan perlunya standarisasi proses bisnis pengelolaan UMKM Teh Mbok Murti. Hasil yang didapatkan dari kegiatan tersebut masyarakat mendapat kemanfaatan yang semakin berkembangnya pemanfaatan kebun teh, terinformasinya keberadaan Bumi Perkemahan Desa Segorogunung melalui berbagai media digital, dan terimplementasinya standarisasi proses bisnis UMKM Teh Mbok Murti.