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All Journal Majalah Kedokteran Bandung e-Journal Pustaka Kesehatan Majalah Geografi Indonesia Folia Medica Indonesiana Journal of Agromedicine and Medical Sciences Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva UNEJ e-Proceeding MNJ (Malang Neurology Journal) Journal of Dentomaxillofacial Science AKSONA
Azham Purwandhono
Departement Of Neurology, Faculty Of Medicine, University Of Jember
Dentistry Earth & Planetary Sciences Education Health Professions Library & Information Science Medicine & Pharmacology Neuroscience Other

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Journal : UNEJ e-Proceeding

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The Effect of Bitter Melon Extract (Momordica charantia)in Inhibition of NFkB Activation in Leptin treated HUVECS Purwandhono, Azham; Bumi, Candra
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

The obesityincreases the riskof morbidityandmortalityof type2diabetesandcardiovasculardisease. Leptin, an adipocytokine that regulate metabolic process, was increase in obesity. Hyperleptinemia activate the transcriptionfactorNuclearFactorKappaB(NF-kB), leading to the occurrence of inflammation. The chronic inflammation in vessels would cause the dysfunction of endothelial cell.Bitter melon (Momordica charantia) has beenused by many peoplefor the treatmentof diseases. Studies showthatbitter melon fruitcontainssaponins(charantins) andalkaloids. This study aimed toinvestigate the effect of bitter melon extract on the level of activated NF-κB. Cultured endothelial cell, HUVECs (Human Umbilical Vein Endothelial), is used for a model of endothelial cells. There are 5 groups: 1) HUVECs culture without leptin as negative control; 2) HUVECs culture with leptin as positive control; and 3 groups given various dosages of bitter melon (0.1 mg/ml; 0.05 mg/ml; 0.01 mg/ml; 0.005 mg/ml; 0.001 mg/ml) and Leptin. Leptin (500 ng/ml) is added on HUVECs to stimulate responds of endothelial cells mimicking obesity condition. Momordica charantiawere given 2 h before leptin treatment. The level of activated NF-KB was measured by ELISA. This study showed that leptin significantly induce NFkB activation and the treatment of bitter melon inhibit NFkB activation significantly (p<0.05). Keywords: Leptin, Endothel, bitter melon, NFkB
The Effect of Bitter Melon Extract (Momordica charantia)in Inhibition of NFkB Activation in Leptin treated HUVECS Azham Purwandhono; Candra Bumi
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The obesityincreases the riskof morbidityandmortalityof type2diabetesandcardiovasculardisease. Leptin, an adipocytokine that regulate metabolic process, was increase in obesity. Hyperleptinemia activate the transcriptionfactorNuclearFactorKappaB(NF-kB), leading to the occurrence of inflammation. The chronic inflammation in vessels would cause the dysfunction of endothelial cell.Bitter melon (Momordica charantia) has beenused by many peoplefor the treatmentof diseases. Studies showthatbitter melon fruitcontainssaponins(charantins) andalkaloids. This study aimed toinvestigate the effect of bitter melon extract on the level of activated NF-κB. Cultured endothelial cell, HUVECs (Human Umbilical Vein Endothelial), is used for a model of endothelial cells. There are 5 groups: 1) HUVECs culture without leptin as negative control; 2) HUVECs culture with leptin as positive control; and 3 groups given various dosages of bitter melon (0.1 mg/ml; 0.05 mg/ml; 0.01 mg/ml; 0.005 mg/ml; 0.001 mg/ml) and Leptin. Leptin (500 ng/ml) is added on HUVECs to stimulate responds of endothelial cells mimicking obesity condition. Momordica charantiawere given 2 h before leptin treatment. The level of activated NF-KB was measured by ELISA. This study showed that leptin significantly induce NFkB activation and the treatment of bitter melon inhibit NFkB activation significantly (p<0.05). Keywords: Leptin, Endothel, bitter melon, NFkB
Co-Authors . Suryono Achmad Maududie Adji, Novan Krisno Alif Mardijana Angga Mardro Raharjo Bagus Hermansyah Banun Kusumawardani Barus, Marsha Zahra Adyanda Candra Bumi Cholis Abrori, Cholis Desie Dwi Wisudanti Diana Chusna Mufida Elly Nurus Sakinah, Elly Nurus Elvia Rahmi Marga putri ENNY SUSWATI Habibi, Ali Handoko, Adelia Heni Fatmawati Jauhani, Muhammad Afiful Jauhar Firdaus Komang Yunita Wiryaning Putri Kristianningrum Dian Sofiana Kristianningrum Sofiana Kusumastuti, Inke M. Ihwan Narwanto Muhammad Afiful Jauhani Muhammad Yudha Nugraha Novan Krisno Adji Pangestu, Ahmad Yudho Hadi Puspita Sari Putri, Elvia Rahmi Marga Putu Ayu Laksmi Lestari Rena Normasari Salsabila Maula Zalfa El Hamzah Syafira, Syafira Tartila, Jasmine Wahyu Ikhsan Wiwien Sugih Utami Yuliana Mahdiyah Daat Arina Yunita Armiyanti Zahrah Febianti Zahrah Febianti