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All Journal Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Biotropika JC-T (Journal Cis-Trans): Jurnal Kimia dan Terapannya JRST (Jurnal Riset Sains dan Teknologi) Jurnal Farmasi Medica (Pharmacy Medical Journal) PMJ JSMARTech : Journal of Smart Bioprospecting and Technology Berkala Penelitian Hayati Pharmademica: Jurnal Kefarmasian dan Gizi Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Makara Journal of Science Jurnal Tumbuhan Obat Indonesia Biosaintifika: Journal of Biology & Biology Education
Ernanin Dyah Wijayanti
Akademi Farmasi Putra Indonesia Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences

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Eksplorasi Ekstrak Etanol Beberapa Tumbuhan Berpotensi Sebagai Antiketombe Ernanin Dyah Wijayanti; Endang Susilowati
JRST (Jurnal Riset Sains dan Teknologi) Volume 1 No. 2 September 2017: JRST
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (619.586 KB) | DOI: 10.30595/jrst.v1i2.1671

Abstract

Abstrak Ketombe merupakan salah satu masalah rambut yang disebabkan oleh fungi Malassezia furfur sehingga untuk pengendaliannya diperlukan agen antifungi. Beberapa tumbuhan telah digunakan secara empiris untuk terapi antiketombe antara lain: inggu (Ruta angustifolia), bambu tali (Gigantochloa apus), johar (Senna siamea), jintan hitam (Nigella sativa), apukat (Persea americana), dadap serep (Erythrina lithosprema), nagasari (Palaquium rostratum), pisang (Musa paradisiaca), sidaguri (Sida rhombifolia), tomat (Solanum lycopersicum), kethuk (Alocasia macrorrhiza) dan komak (Dolichos lablab). Penelitian ini bertujuan untuk membuktikan aktivitas antifungi ekstrak etanol tumbuhan uji dan menentukan nilai Kadar Hambat Minimum (KHM) dan Kadar Bunuh Minimumnya (KBM). Masing-masing simplisia tumbuhan uji diekstraksi dengan metode maserasi menggunakan pelarut etanol 70% selama 5 hari. Pengujian aktivitas antifungi terhadap Malassezia furfur menggunakan metode difusi sumuran, yang dilanjutkan dengan penentuan KHM dan KBM dengan metode dilusi. Hasil penelitian menunjukkan bahwa semua ekstrak etanol tumbuhan uji memiliki aktivitas antifungi yang bervariasi terhadap Malassezia furfur. Aktivitas yang kuat ditunjukkan oleh ekstrak etanol kulit buah pisang, daun bambu tali, daun komak, biji jintan hitam dan daun inggu. Nilai KHM masing-masing ekstrak antara lain: kulit buah pisang, daun bambu tali dan daun komak (10%), biji jintan hitam (20%), daun inggu (30%), daun dadap serep dan daun johar (50%), daun tomat (60%), daun apukat dan daun nagasari (70%). Ekstrak etanol daun sidaguri dan daun kethuk tidak memiliki nilai KHM. Semua ekstrak etanol tumbuhan uji tidak memiliki nilai Kadar Bunuh Minimum (KBM). Kata kunci: antifungi, ekstrak etanol, ketombe Abstract Dandruff is one of hair problem caused by Malassezia furfur, which can be controlled by antifungal agents. Empirically, some plants have been used as antidandruff treatment: rue (Ruta angustifolia), bamboo tali (Gigantochloa apus), blackwood cassia (Senna siamea), black cummin (Nigella sativa), avocado (Persea americana), Indian coral tree (Erythrina lithosprema), gutta percha (Palaquium rostratum), banana (Musa paradisiaca), cuban jute (Sida rhombifolia), tomato (Solanum lycopersicum), taro (Alocasia macrorrhiza) and lablab (Dolichos lablab). This research was aimed to observe antifungal activity, Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) of these 12 potential plants against Malassezia furfur. Each of plant simplisia was extracted by immersion in 70% ethanol  for 5 days. Antifungal activity assay against Malassezia furfur were conducted using agar well diffusion, followed by dilution method to determine MIC and MFC. The results showed that all of plant ethanolic extract have various antifungal activity against Malassezia furfur. Strong activity showed by banana peels extract, bamboo tali leaves extract, lablab leaves extract, black cumin seeds extract and rue leaves extract. MIC values obtained were 10 % for banana peels extract, bamboo tali and lablab leaves extract,  20% for black cumin seeds extract, 30% for rue leaves extract, 50% for Indian coral and blackwood cassia leaves extract, 60% for tomato leaves extract, 70% for avocado and gutta percha leaves extract. Whereas cuban jute and taro leaves extract did not show MIC values. All of plant ethanolic extracts did not show MFC values.  Key words: antifungal, dandruff, ethanolic extract
Antimicrobial Activity of Ferulic Acid in Indonesian Purple Rice through Toll-like Receptor Signaling Wijayanti, Ernanin Dyah; Safitri, Anna; Siswanto, Dian; Triprisila, Lidwina Faraline; Fatchiyah, Fatchiyah
Makara Journal of Science Vol. 25, No. 4
Publisher : UI Scholars Hub

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Abstract

Purple rice is a potential source of ferulic acid, which has antimicrobial properties. However, the inhibitory mechanism of ferulic acid on the growth of bacteria, particularly Salmonella and Listeria, has not been elucidated. This study aimed to determine the bioactivity of ferulic acid from purple rice as an antimicrobial agent against Salmonella typhimurium and Listeria monocytogenes using in vitro and in silico analyses. The antimicrobial activity of a purple rice ferulic acid extract was tested using the agar well diffusion method. Its effect on bacterial cells was observed using scanning electron microscopy. Ferulic acid was confirmed to have antimicrobial properties using in silico software to attenuate the binding of bacterial virulence factors (lipoproteins, lipopolysaccharides, and flagellins) to Toll-like receptors (TLRs) and to prevent interactions with peptidoglycans. The purple rice ferulic acid extract inhibited bacterial growth. The inhibitory effects included induction of a biofilm and shrinkage of S. Typhimurium, as well as osmotic lysis of L. monocytogenes. This activity was supported by the ability of ferulic acid to inhibit the binding of bacterial virulence factors with TLRs and block bacterial peptidoglycans. These findings indicate that the purple rice ferulic acid extract acts as antimicrobial, both directly to bacterial cells and indirectly through TLRs. We conclude that ferulic acid from Indonesian purple rice has a biological function as an antimicrobial agent.
Kombucha Batang Pisang Kepok: Kadar Fenolik Total, Aktivitas Antibakteri, dan Pengaruh terhadap Viabilitas Lactobacillus gasseri Nur Laela; Fitri Husniatin Nisa; Riki Purwosutanto; Nur Hayati; Ernanin Dyah Wijayanti
PHARMADEMICA : Jurnal Kefarmasian dan Gizi Vol 2 No 2 (2023): Oktober - Maret
Publisher : LPPMKI - AKAFARMA AKFAR PIM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54445/pharmademica.v2i2.32

Abstract

Kepok banana pseudo stem (Musa acuminate balbisiana Colla) contains several secondary metabolites which have potential as antioxidants and antibacterials. Fermentation is known to increase the levels of phenolic compounds and their bioactivity. This study aims to analyze the effect of kombucha fermentation of kepok banana pseudo stems on total phenolic content, antibacterial activity and its effect on the viability of Lactobacillus gasseri. Banana pseudo stems were fermented with kombucha culture for 12 days at room temperature, the total phenolic content was determined by the Folin-Ciocalteu method, the antibacterial activity was tested by the well diffusion method, while the viability of L. gasseri was determined by the Total Plate Count (TPC) method. The total phenolic content of kepok banana stems increased by 60.88% after being fermented with kombucha, but the kombucha of kepok banana pseudo stems did not show antibacterial activity against Escherichia coli and caused a decrease in the viability of L. gasseri. This study shows that kombucha fermentation is beneficial for increasing phenolic levels and has potential as an antibacterial, especially against Gram positive groups.
Phytochemical Profile and Antioxidant Potential of Tobacco Flower Extract in Different Solvents Wijayanti, Ernanin Dyah; Rahayu, Lina Oktavia; Dzulaikha, Shovia; Ayunda, Fiddini Robetta; Djara, Selfiana
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 1 (2024): JSMARTech Volume 5, No. 1, 2024
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.01.04

Abstract

Tobacco (Nicotiana tabacum) is a commercial plant that is extensively used, particularly for its leaves. Thus far, exploration on the flower part is still limited. The objective of this study was to evaluate the phytochemical profile and antioxidant activity of tobacco flower extracts in different solvents. Tobacco flowers were macerated separately in methanol, ethanol, and n-hexane. Each extract was assayed by color reaction test to detect the phytochemical content. Total phenolic content (TPC) and antioxidant activity were determined by Folin-Ciocalteu and DPPH methods, respectively. Flavonoids, saponins, alkaloids, and triterpenoids are detected in methanol and ethanol extract, anthraquinones is only detected in methanol, while glycosides and steroids are only detected in n-hexane extracts. Among the other extracts, the methanol extract was the richest in phytochemical compounds and had the highest TPC (132.589±0.662 mgGAE/g). However, tobacco flower extract in all solvents were less effective as antioxidant due to very high IC50 values (>500 µg/mL).
Fermented Black Rice Extract (Oryza sativa) Induces Morphological Changes in Pathogenic Microorganisms Wijayanti, Ernanin Dyah; Syafah, Lailiiyatus; Rahayu, Lina Oktavia; Wulandari, Dita; Ibrahim, Zalikha
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 1 (2025): April 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i1.17185

Abstract

Black rice (Oryza sativa L.) contains nutrients and bioactive compounds with potential application in health, including antimicrobial properties. To date, the application of fermentation to black rice to enhance its bioactivity, particularly as an antimicrobial, remains unclear. This study aims to evaluate the antimicrobial activity of solid fermented black rice against Gram-positive bacteria, Gram-negative bacteria, and fungi. Black rice was fermented using Saccharomyces cerevisiae under solid-state conditions. Extraction was carried out by maceration using 96% ethanol. Antimicrobial activity was assessed using the well diffusion test method, agar dilution, and scanning electron microscope (SEM). Fermented black rice extract showed antimicrobial activity against all tested microbes. The minimum inhibitory value obtained was 20% against all microbes, but the minimum bactericidal/fungicidal concentration (MBC/MFC) could not be determined. The SEM analysis showed morphological changes in microbes exposed to fermented black rice extract, including cell shrinkage, elongation, and lysis in Bacillus cereus, fragmentation and irregular cell shape in Escherichia coli, cell size reduction in Staphylococcus aureus, and bleb formation in Candida albicans. These changes indicate the mechanism of microbial growth inhibition. We found that fermented black rice (EFBR) extracts produce different effects from unfermented black rice extracts (EUBR), which tend to induce biofilm formation. We propose that EFBR functions as an antimicrobial by rupturing cell walls, preventing cell division and DNA synthesis. This research provides new insights into natural antimicrobial mechanisms at the cellular level and offers a potential alternative for addressing antimicrobial resistance. Solid fermented black rice may serve as a valuable source of antimicrobial compounds for developing health products or natural preservatives.
Edukasi dan Pendampingan Pengelola Sayur Organik Baron Agro Lesanpuro Kota Malang Raharjo, Sentot Joko; Wijayanti, Ernanin Dyah
TRI DHARMA MANDIRI: Dissemination and Downstreaming of Research to the Community (Journal of Community Engagement) Vol 1 No 1 (2021)
Publisher : SMONAGENES Research Center, Univeritas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2021.001.01.17

Abstract

Lesanpuro merupakan salah satu wilayah yang memiliki potensi dan daya tarik tersendiri karena memiliki hasil alam yang cukup dan luas lahan pertanian. Hal tersebut membuat wilayah tersebut menjadi penyumbang potensi yang berpengaruh bagi komoditas pertanian kota Malang, khususnya sayur organik dan buah yang sudah sangat jarang sekali ditemui lahan pertanian di tengah kota, namun kelompok usaha ini belum memahami benar arti pentingnya cara produksi yang baik dan keamanan produk pangan sayur organik. Edukasi dan pendampingan kelompok usaha pengolahan sayur organik yang benar dan pengetahuan meliputi semua aspek penanganan bisnis tersebut. Tujuan pengabdian masyarakat ini adalah edukasi dan pendampingan kelompok usaha dalam pengelolaan sayur organik yang baik agar menjadi produk pangan yang aman sehingga menjadi peluang usaha baru untuk meningkatkan kesejahteraan dan kemandirian dalam lingkungan, kesehatan, dan perekonomian. Metode yang digunakan adalah PAR (Participatory Action Research) berupa penyuluhan, pelatihan, demonstrasi, serta pendampingan. Pendampingan dilakukan selama 3 bulan. Hasil pendampingan menunjukkan bahwa 95% responden setuju bahwa kegiatan pengabdian kepada masyarakat bermanfaat, 89% responden setuju kegiatan edukasi ini sangat meningkatkan kualitas, 97% responden menyatakan ilmu bertambah setelah mengikuti pelatihan, dan 85% responden menyatakan bahwa keseluruhan kegiatan pelatihan sudah baik. Kesimpulan pengabdian adalah kegiatan edukasi dan pendampingan kelompok usaha dalam pengelolaan sayur organik agar menjadi produk pangan yang aman mendapatkan respons yang baik dan menjadi peluang usaha baru untuk meningkatkan kesejahteraan dan kemandirian dalam lingkungan, kesehatan, dan perekonomian.
Pemberdayaan Kelompok Ibu PKK Desa dalam Penyuluhan Menu Nutrisi Sehat Berbasis Produk Lokal di Desa Karangwidoro, Kecamatan Dau, Kabupaten Malang Fatchiyah, Fatchiyah; Suyanto, Eko; Wijayanti, Ernanin Dyah; Husnah, Yeni Avidhatul
TRI DHARMA MANDIRI: Dissemination and Downstreaming of Research to the Community (Journal of Community Engagement) Vol 2 No 2 (2022)
Publisher : SMONAGENES Research Center, Univeritas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2022.002.02.70

Abstract

Berdasarkan hasil penelitian riset kesehatan dasar, konsumsi pangan masyarakat Indonesia saat ini sebagian besar belum sesuai nutrisi sehat dan seimbang khususnya konsumsi sayuran dan buah-buahan. Hal ini menimbulkan kurangnya implementasi konsumsi pangan sehat masyarakat desa. Penyuluhan dan edukasi menu nutrisi sehat berbasis produk lokal menjadi penting dilakukan untuk mendukung Gerakan Masyarakat Hidup Sehat (GERMAS) serta pemanfaatan pangan lokal yang bernutrisi sehat. Salah satu desa dengan potensi sumber daya lokal yang melimpah sebagai produk lokal pangan nutrisi sehat adalah Desa Karangwidoro, Kecamatan Dau, Kabupaten Malang. Kegiatan penyuluhan ini merupakan upaya pemenuhan program pengabdian kepada masyarakat DPP/SPP Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Brawijaya Tahun 2022 untuk memberikan edukasi bagi masyarakat khususnya ibu-ibu PKK sebagai penggerak pertama dalam keluarga. Metode pelaksanaan dilakukan dengan 5 tahap yaitu kerangka pemecahan masalah; diskusi; sosialisasi penyuluhan; khalayak sasaran yakni perangkat desa, ketua pokja sandang dan kesehatan, ibu-ibu PKK; serta pelaksanaan kegiatan. Bentuk penyuluhan berupa edukasi pangan sehat, praktik menu sehat dan pengolahannya, serta pemaparan materi. Kegiatan penyuluhan dan edukasi menu sehat berjalan sukses dengan ibu-ibu kader PKK desa yang sangat antusias. Hasil kegiatan pre-test menunjukkan nilai 65–70 sebanyak 33%. Hasil post-test terdapat peningkatan menjadi 91–100 sebanyak 45%. Pendampingan dan pemantauan dilakukan oleh kader PKK kesehatan dan tim pengabdi di masyarakat secara door to door untuk mendukung keberhasilan program.
Characterization of Yellow Tabebuia Flower Extract (Tabebuia aurea) Nanoparticles and Bioactivity as Antioxidant: Karakterisasi Nanopartikel Ekstrak Bunga Tabebuya Kuning (Tabebuia aurea) dan Bioaktivitas sebagai Antioksidan Yunita, Yunita; Syarifah, Anisa Lailatusy; Rahayu, Lina Oktavia; Wijayanti, Ernanin Dyah
Jurnal Tumbuhan Obat Indonesia Vol. 18 No. 1 (2025): July 2025
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jtoi.v18i1.2174

Abstract

Degenerative diseases can be prevented by natural antioxidants in plant. Yellow tabebuia flower is natural antioxidants. Plant extracts can develope into green synthesis nanoparticle to increase the effectiveness as medicinal materials. This research aims to determine the characteristics of yellow tabebuia flower extract nanoparticles and bioactivity as antioxidants. The methods included preparation and extraction of simplisia using ethanol solvent by maceration and sonication. The extract was determined flavonoid and phenol content by colorimetric method and antioxidant activity by DPPH method. The extract was also made into nano particles by ionic gelation method, with variations addition of tween 80 as much as 0.5, 0.75 and 1 ml. The results of this research are extract has a yield 13.09%, contains flavonoids, tannins, phenolics, alkaloids, saponins and terpenoids. The value of total flavonoid content is 0.59%, total phenolic content is 3.11% and antioxidant activity test has IC50 value of 165.26. The use ionic gelation method can reduce particle size. Three formulas that made the variation surfactant addition has a particle size of 47-283 nm, in addition tween80 1 ml has the smallest particle size of 47 nm with a zeta potential value of 39.3 mV. Keywords: yellow tabebuia flower extract, nanoparticles, antioxidant activity
Molecular Interaction of Purple Rice Ferulic Acid and Skin Aging-Related Enzymes Wijayanti, Ernanin Dyah; Fatchiyah, Fatchiyah; Safitri, Anna; Siswanto, Dian
Makara Journal of Science Vol. 29, No. 1
Publisher : UI Scholars Hub

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Abstract

Purple rice is rich in ferulic acid, a phenolic acid abundant in rice. Ferulic acid (FA) has long been recognized for its anti-oxidant properties, which are linked to its skin anti-aging activity owing to its ability to scavenge free radicals and pro-tect cells from damage. Skin anti-aging activity could be assessed by inhibiting skin aging enzymes. However, there was limited information on the mechanism of FA inhibition of these enzymes. This study focused on the molecular interac-tion between purple rice FA and collagenase or tyrosinase, both with and without their substrates. The interaction be-tween FA (CID 445858), collagenase (2Y6I), tyrosinase (5M8M), collagenase substrate (CID 5486160), and tyrosinase substrate (CID 6057) was docked using Hex 8.0.0 Cuda with the default settings. Discovery Studio 2020 and LigPlot software were used to visualize the docking results. Ascorbic acid (CID 54670067) and kojic acid (CID 3840) were used as positive controls for collagenase and tyrosinase, respectively. Molecular docking revealed that FA formed electro-static, hydrogen, and hydrophobic bonds with all aging enzymes, substrates, and enzyme–substrate complexes. FA could bind to both free enzymes and free substrates, consequently attenuating enzyme–substrate bonding, and preventing the substrate from binding appropriately in its normal position. Molecular dynamics simulations demonstrated that FA formed a stable bond with tyrosinase, similar to kojic acid, although the bond with collagenase was less stable. However, the pattern of each parameter during the simulation was comparable with that of ascorbic acid. The current study indicated that the FA from purple rice had the potential to act as an inhibitor of skin aging en-zymes and could be developed as a skin anti-aging candidate
Genomic Profile of OsCOMT in Indonesian Purple Rice Wijayanti, Ernanin Dyah; Safitri, Anna; Siswanto, Dian; Fatchiyah, Fatchiyah
Biotropika: Journal of Tropical Biology Vol. 10 No. 3 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.03.04

Abstract

The Indonesian purple rice (IPR), a crossbreeding of black and white rice, is a potential source of ferulic acid. Up to this point, the genomic similarity between the crossbreeding and its parentals has not been determined, particularly in genes involved in ferulic acid biosynthesis. In this present work, we analysed the profile of Oryza sativa caffeic acid-O-methyltransferase (OsCOMT) gene in IPR. The genomic DNA was extracted by CTAB method, then amplified with a specific primer of OsCOMT gene. The amplicon was sequenced by Sanger method and confirmed by geneID NC_029259.1. The IPR showed an amplicon of 708 bp sequence of the OsCOMT gene, indicating the gene involved in ferulic acid biosynthesis. The IPR gene profile is similar to the parentals, but there are variations in point mutations that distinguish it from the parentals. Aspartic acid was changed to Glutamine by mutations at positions 52, 69, and 79. We suggest that IPR has a novel OsCOMT gene variant that is unique as it is the crossbreed.
Co-Authors Afriliana, Dwi Agustanti, Khalimatus Nur Eka Ayunda, Fiddini Robetta Dian Siswanto Djara, Selfiana Dzulaikha, Shovia Eko Suyanto Elrica Maggian Murelina Elwin Dwi Novitasari Endang Susilowati fatchiyah . Fatchiyah Fatchiyah Fitri Husniatin Nisa Hardiansi, Fitri Husnah, Yeni Avidhatul Ibrahim, Zalikha Lidwina Faraline Triprisila Linda Jaushica Yustin Munteira, Anita Noor Annisa Susanto Nur Hayati Nur Laela nurmiati Dyah nurmiati Oktavina Kartika Putri Rahayu, Lina Oktavia Riki Purwosutanto Safitri, Anna Sentot Joko Raharjo Setiawan, Nur Candra Eka Syafah, Lailiiyatus Syarifah, Anisa Lailatusy Tri Ardyati Uswatun, Uswatun Wulandari, Dita Yunita Yunita