Article Per Year (5 Year)
p-Index From 2021 - 2026
1.789
P-Index
This Author published in this journals
All Journal Buletin Peternakan VALENSI Jurnal Lahan Suboptimal AL KAUNIYAH Biogenesis: Jurnal Ilmiah Biologi Indonesian Journal of Agricultural Science Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Jurnal Sumberdaya Hayati Livestock and Animal Research Jurnal Biodjati Jurnal Sains Teh dan Kina Jurnal Kimia Terapan Indonesia Jurnal Mikologi Indonesia Indonesian Aquaculture Journal Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Warta AKAB Makara Journal of Science
Nani Radiastuti
Program Studi Biologi, FST Universitas Islam Negeri Syarif Hidayatullah, Jakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Veterinary Other

Published : 28 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 28 Documents
Search

 1   2   3 
ISOLATION AND IDENTIFICATION OF BACTERIA REMOVING NITRITE, NITRATE, AND AMMONIUM FROM BIOBALLS FILTER Qoriatul Ilma; Achmad Dinoto; Ninu Setianingrum; Mulyadi Mulyadi; Dwi Agustyani; Nani Radiastuti; Heddy Julistiono
Indonesian Aquaculture Journal Vol 17, No 1 (2022): (June, 2022)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.17.1.2022.13-22

Abstract

The presence of effective bacteria removing nitrite, nitrate, and ammonia in a recirculating aquaculture system (RAS) is necessary to attenuate their toxicity to fish. The research was conducted to find bacteria that can be cultured and reduce nitrite, nitrate, and ammonium. Sixteen bacterial colonies were isolated from bioballs of RAS biofilter and tested for their ability to reduce nitrite or nitrate concentrations. Using a simple indicator paper for nitrite and nitrate, four isolates that reduced nitrite and nitrate concentrations, namely K1NA3, K2NA3, CNA1, and PRO4NA1 were selected. The four isolates were then evaluated for the metabolism of nitrate, nitrite, and ammonium compounds using the spectrophotometry method. Results showed that the isolates K1NA3, CNA1, and PRO4NA1 reduced nitrite concentration but produced ammonium, whereas K1NA3 isolate was able to reduce nitrate concentration but produced both nitrite and ammonium. Experiments in reducing ammonium levels in the synthetic waste media showed the ability of four isolates to reduce ammonium levels after six days despite producing nitrite. Based on the 16S rRNA gene analysis, these isolates have a close relationship to Pseudomonas otitidis (KINA3 and K2NA3), Acinetobacter cumulans (CNA1), and Vogesella perlucida (PRO 4NA1).
Fungal Community of Culturable Fungal Endophytes Associated with Cinchona calisaya Collected from Gambung, West Java, Indonesia Hidayat, Iman; Radiastuti, Nani; Rahayu, Gayuh; Okane, Izumi
Makara Journal of Science Vol. 23, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Previous studies regarding endophytic fungi associated with Cinchona were focused on the analysis of quinine and related fungal metabolites. In the current study, the community structure of culturable endophytic fungi associated with C. calisaya (i.e., leaf, petiole, twig, root, flower, and fruit) were analyzed and elucidated. Representative isolates from various morphotypes were identified based on a sequence generated from the Internal Transcribed Spacer (ITS) rDNA region. A total of 638 fungal strains were isolated from 700 plant segments. All isolates belong to the phylum Ascomycota and are dominated by a member of Sordariomycetes. The fungal endophyte community within C. cali-saya consists of 23 known species, 10 species complexes, and 6 unidentified species. Based on the Shannon-Wiener diversity index, the leaves and fruits host the most diverse endophytic fungi, followed by twigs, petioles, and bark. Members of the genus Diaporthe are commonly found in all C. calisaya organs. Neofussicoccum sp. was only one found within twigs; the Colletotrichum gloeosporioides complex and Guignardia mangiferae were only found within leaves; the C. brasiliense complex was detected in flowers; and Fusarium oxysporum is specific to roots.
The quality of liquid biostimulant formula of microbial-based during packaging and storage Radiastuti, Nani; Susilowati, Dwi Ningsih; Rahayu, Rizkiyah Putri; Kosasih, Jajang; Tentrem, Titi
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 1 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i1.42159

Abstract

.
Karakterisasi dan Potensi Antibakteri Ekstrak Kapang Endofit Hasil Isolasi dari Sargassum sp.: Characterization and Antibacterial Potential of Endophytic Fungi Extracts Isolated from Sargassum sp. Radiastuti, Nani; Bahri, Saiful; Muhammad, Alfianur Azmi; Dwikencana, Windy; Haribowo, Dinda Rama; Solihat, Nur Amaliah; Ramadhan, Firdaus; Ridhwan, Muhammad
Jurnal Sumberdaya Hayati Vol. 10 No. 4 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.4.183-189

Abstract

The bioactive potential in Sargassum sp. and its direct use is expected to reduce its population in nature if used continuously. Using endophytic fungi from the host is a solution for obtaining bioactive compounds from Sargassum sp. The purpose of this study was to characterize endophytic fungi in Sargassum sp. and its potential as an antibacterial agent. Endophytic fungi were isolated using the direct planting technique, endophytic fungi were characterized through microscopic and macroscopic observations. Extraction was performed with two organic solvents, namely ethanol and ethyl acetate. Antibacterial potency was tested using the agar diffusion method using Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Salmonella typhi. Based on the results, two mold isolates, D3P1 and D4P1, were obtained. The ethyl acetate extract of D3P1 had the highest inhibition zone for Bacillus subtilis bacteria, with the highest inhibition zone being 23.2 mm for Bacillus subtilis and the smallest being 17 mm for Escherichia coli. The ethyl acetate extract of D3P1 had the highest inhibition zone compared to the ethyl acetate extract of D4P1 and the ethanol extract of D3P1 and D4P1. The GC-MS analysis identified four compounds that have been identified in the ethyl acetate extract of D3P1 isolates, among others, 2,3-Butanediol, Phthalic acid, 1,2-Benzenedicarbocylic acid, and 1-Anthracenamine. Phthalic acid and 1,2-Benzenedicarboxylic acid compounds have the potential as antibacterial.
Bioproses Penyisihan Amonia Pada Air Baku Instalasi Pengolahan Air Cilandak oleh Konsorsium Bakteri Endogenus dan Eksogenus (Skala Laboratorium) Sunandar, Ibnu Hasan; Maddona, Sandra; Radiastuti, Nani
Journal Warta AKAB Vol 49, No 1 (2025): Juli 2025
Publisher : Politeknik AKA Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55075/wa.v49i1.239

Abstract

Pencemaran amonia di Sungai Krukut, yang menjadi sumber air baku Instalasi Pengolahan Air Minum (IPAL) Cilandak, menimbulkan tantangan besar dalam menjaga kualitas air. Penelitian ini bertujuan untuk mengevaluasi efisiensi penyisihan amonia menggunakan konsorsium bakteri endogenus dari Sungai Krukut dan bakteri eksogenus dari koleksi Sekolah Ilmu Teknologi Hayati ITB. Penelitian dilakukan dalam skala laboratorium dengan dua perlakuan utama selama 72 jam. Hasil menunjukkan bahwa perlakuan dengan bakteri eksogenus menghasilkan efisiensi penyisihan amonia tertinggi, yaitu 20,17%, sedangkan konsorsium bakteri endogenus mencapai efisiensi 7,9%. Analisis statistik menunjukkan bahwa perbedaan antara kedua perlakuan tidak signifikan secara statistik (p 0,05). Kedua konsorsium bakteri mampu tumbuh dalam lingkungan air baku dengan parameter kualitas air yang masih melebihi baku mutu yang ditetapkan. Temuan ini menggarisbawahi potensi penggunaan konsorsium bakteri, terutama bakteri eksogenus, untuk meningkatkan kualitas air baku melalui penyisihan amonia secara biologis. Penelitian ini memberikan kontribusi dalam pengembangan metode pengolahan air yang ramah lingkungan dan efektif untuk mengatasi pencemaran amonia di sumber air baku.
Isolation And Characterization of Bacteria from Shallots (Allium cepa L.) as In-vitro Biocontrol Agent of Fusarium oxysporum f.sp cepae Radiastuti, Nani; Amalia, Wuliani; Suryadi, Yadi; Susilowati, Dwi N; Kosasih, Jajang
Al-Kauniyah: Jurnal Biologi Vol. 17 No. 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i2.38132

Abstract

AbstractShallot is one of the leading vegetable commodities with many benefits such as for seasonings and herbal medicinal ingredients. The demand for shallots continues to increase; however, shallot production is still relatively low. One of the limiting factors causing low shallot production is due to wilt disease caused by Fusarium oxysporum f.sp cepae (Foc). Bacteria have many roles in suppressing the growth of Foc, and this study aims to obtain potential bacterial isolates from the shallot plant to inhibit the growth of Foc Based on fungal diameter zone inhibition, degree of inhibition, and chitinase test, it was obtained 9 isolates which could suppress the growth of Foc. The results indicated that the AB3, TB2, and UB1 bacterial isolates could inhibit the growth of Foc with a percentage of inhibition of 46.80; 40.24; and 35.11%, respectively. The analysis showed that AB3, TB2, and UB1 isolates were categorized as moderate in suppressing the growth of Foc. The 16S rRNA sequencing results showed that AB3 and TB2 isolate had similarities with Bacillus subtilis by 99,75%, and 100%, respectively, while UB1 isolate had similarities with Pseudomonas nitroreducens by 89,35%. Based on the result showed that Bacillus sp. AB3 and TB2 isolates, and P. nitroreducens UB1 isolate have more potential as biological control agents to control the Fusarium wilt at in vitro assay. The field efficacy studies on these potential antagonists need to be done in the future.AbstrakSalah satu faktor pembatas yang menyebabkan rendahnya produksi bawang merah adalah penyakit layu yang disebabkan oleh Fusarium oxysporum f.sp cepae (Foc). Bakteri antagonis memiliki banyak peran dalam menekan pertumbuhan Foc, dan penelitian ini bertujuan untuk mendapatkan isolat bakteri antagonis potensial asal tanaman bawang merah untuk menghambat pertumbuhan Foc. Berdasarkan nilai zona hambat diameter jamur, derajat hambat dan uji kitinase, diperoleh 9 isolat yang dapat menekan pertumbuhan Foc. Hasil penelitian menunjukkan bahwa isolat bakteri AB3, TB2, dan UB1 mampu menghambat pertumbuhan Foc dengan persentase penghambatan masing-masing sebesar 46,80; 40,24; dan 35,11% dengan kategori penghambatan pertumbuhan Foc moderat. Hasil sekuensing 16S rRNA, menunjukkan bahwa isolat AB3 dan TB2 memiliki kemiripan dengan Bacillus subtilis masing-masing sebesar 99,75%, dan 100%, sedangkan isolat UB1 memiliki kemiripan dengan Pseudomonas nitroreducens sebesar 89,35%. Berdasarkan hasil penelitian menunjukkan bahwa Bacillus sp. isolat AB3, TB2, dan P.nitroreducens isolat UB1 berpotensi digunakan sebagai agen pengendali hayati untuk mengendalikan penyakit layu Fusarium pada uji in vitro. Studi kemanjuran lapangan terhadap isolat antagonis potensial ini perlu dilakukan di masa depan.
In Vivo Testing of Pseudozyma hubeiensis to Control Aspergillus flavus in Nutmeg Seeds (Myristica fragrans Houtt.) Rahayuningsih, Sri; Hidayah, Mudrikah Nurul; Susilowati, Dwi Ningsih; Supriadi; Suryadi, Yadi; Rismayani; Radiastuti, Nani
Al-Kauniyah: Jurnal Biologi Vol. 19 No. 1 (2026): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v19i1.46462

Abstract

Nutmeg (Myristica fragrans Houtt.) is a high-value spice commodity from Indonesia, often contamination with aflatoxin-producing Aspergillus flavus. Previous research has identified two epiphytic yeast isolates from nutmeg plants, namely DP1341a and DP1342, both of which were identified as Pseudozyma hubeiensis and showed antagonistic potential against A. flavus. This study is a follow- up study that aims to test the antagonistic activity of both isolates against A. flavus in vivo on nutmeg seeds. The research was conducted experimentally through several stages, namely testing the compatibility of isolates on Yeast Malt Agar (YMA) media, determining the optimum fermentation time, and applying liquid culture and fermentation filtrate to 120 nutmeg seeds. Results showed that the consortium of isolates DP1341a and DP1342 did not increase the inhibitory power results compared to single cultures with optimum fermentation times of 10 and 14 days, respectively. In vivo applications show that treatment with DP1342 isolate (KC-DP1342) and consortium in liquid culture provides the highest level of resistance to A. flavus infection, namely 38.67% and 28.00%. The findings suggest that P. hubeiensis DP1342 has potential as a biological control agent in controlling A. flavus on nutmeg seeds, although further formulation and optimization are needed.
Potency of Papaya Seed Powder (Carica papaya L.) as Chicken Meat and Shrimp Preservative Radiastuti, Nani; Fitri, Reno; Berty, Gusniar
Jurnal Biodjati Vol 5 No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.9267

Abstract

 Chicken meat and shrimp are perishable food materials. Giving natural preservatives is one way to extend the storage time of food. This study aimed to know the potential of papaya seed powder varieties of ‘Bangkok’ and ‘California’ as preservatives. The sample consisted of two varieties of papaya seed powder with concentrations of 2,4,6 and 8 g. Storage time for chicken meat and shrimp was the third days. Parameters measured were total bacterial colonies, pH va-lues, and organoleptic characteristics (color, aroma and texture) of fresh chicken meat and shrimp. The result showed papaya seed pow-der influences the quality of chicken meat and shrimp. fresh chicken meat and shrimp with papaya seed powder varieties ‘Bangkok’ and ‘California’ had a lower total bacterial colony as much as 9 x 105 until 1x106 compared to control which were 4.2 x 107 - 1.87 x 107. Organoleptic result of chicken meat and shrimp with the treatment of papaya seed powder and stored for three days continue to decrease in term of color, aroma and texture. In chicken meat, the decrease in bacterial growth was still within the limits of microorganism con-tamination quality in animal-based food. However, the same con-centrations of papaya seed powder in the shrimp have not been able to reduce bacterial growth until the limit permitted by SNI. 
Co-Authors A Sa’duddin Achmad Dinoto Adeng Hudaya Aina Nadila Alfida Zakiyah, Alfida Amalia, Wuliani Amelia Rakhmaniar Anna Muawanah Berty, Gusniar Dede Sukandar Dianna Rossyta Pratiwi Dwi Agustyani Dwi N Susilowati Dwi N Susilowati DWI NINGSIH SUSILOWATI Dwi Ningsih Susilowati Dwikencana, Windy Elpawati, Elpawati Endang Tri Margawati Endang Tri Margawati F Wijayanti Firdaus Ramadhan Fitri, Reno Gayuh Rahayu Gusniar Berty Hajar Indra Wardhana Heddy Julistiono Hidayah, Mudrikah Nurul Ika Roostika Ika Roostika Tambunan Iman Hidayat Indah Sofiana Indri Garnasih Ira Djajanegara Ira Djajanegara Ira Jayanegara Izumi Okane, Izumi Khoeriyah Khoeriyah Kosasih, Jajang La Ode Sumarlin Lisca Puji Rustanti Maddona, Sandra Muhammad Ridhwan Muhammad, Alfianur Azmi Mulyadi Mulyadi Ninu Setianingrum Nurhasni, N. Qoriatul Ilma R Fathoni Rahayu, Rizkiyah Putri Reno Fitri Rina Ningtiyas Rismayani Saiful Bahri Sena Yunia Saputri Setiyani, Alfi Dwi Slamet Diah Volkandari Slamet Diah Volkandari Sofiana, Indah Solihat, Nur Amaliah Sri Rahayuningsih Sri Rahayuningsih Suminar S. Achmadi Sunandar, Ibnu Hasan Supriadi Suryadi, Yadi Susilowati, Dwi Ningsih Tentrem, Titi Yadi Suryadi