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All Journal Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) Jurnal Penelitian Pendidikan IPA (JPPIPA) Al-Kimia JKPK (Jurnal Kimia dan Pendidikan Kimia) AMALIAH: JURNAL PENGABDIAN KEPADA MASYARAKAT Media Farmasi Indonesia Sewagati: Jurnal Pengabdian Masyarakat Indonesia Jurnal Ilmiah Sains Sabangka Abdimas Jurnal Pengabdian Masyarakat Sabangka Parapemikir Jurnal Ilmiah Farmasi
Rahmawati Salsa Dinurrosifa
Universitas Negeri Semarang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Other

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Analysis Pork Contamination in Beef Meatballs through Polymerase Chain Reaction in Palu City Polibaon, Tresya J.; Mariyani; Tandi, Joni; Handayani, Tien Wahyu; Dinurrosifa, Rahmawati Salsa; Sene, Ikhsan Hi. Amir
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.9006

Abstract

The need for halal products is essential, particularly among various processed beef products like meatballs, which are popular among nearly the entire community. These products are susceptible to issues of meat counterfeiting for greater economic gain. This study aims to determine the smallest DNA concentration that can still be detected and to assess whether there is any pork contamination in meatballs circulating in Palu City using Polymerase Chain Reaction (PCR). This test uses four DNA samples obtained from fresh meat to test the specificity of the primer: wild boar, rat, and goat DNA samples as comparisons, and pig DNA as a positive control. The presence of DNA bands in the agarose gel electrophoresis visualization results indicates that DNA amplification has occurred, meaning that the sample can be detected. The analysis results showed that the primer used (Cyt-b) had good specificity for detecting pork DNA, while the absolute pork DNA concentration of 0.01 ng/µL and the reference meatball DNA with a concentration of 0.01% are the smallest limits that can still be detected using PCR. Of the four meatball samples from Palu City, one sample produced a DNA band, indicating that the sample contains or is contaminated with pig DNA. This method is valid as it meets the specificity test and demonstrates a good detection limit.
Characterization and Effectiveness Testing of a Transdermal Patch Combining Red Betel Leaf Extract and Tapak Liman Extract for the Healing of Diabetic Gangrene Wounds Hanun Aishy Marwa; Nadine Ayu Syaima Gustaf Prins; Alliva Ro’ain Abbiyu Imanita; Fania Putri Wiba Maharani; Rahmawati Salsa Dinurrosifa
Jurnal Penelitian Pendidikan IPA Vol 11 No 12 (2025): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i12.13292

Abstract

This study aimed to develop and evaluate three nanogel-based transdermal patch formulations combining red betel leaf (Piper crocatum) and tapak liman (Elephantopus scaber L.) extracts to accelerate diabetic gangrene wound healing. The extracts were obtained by maceration using 70% ethanol and qualitatively indicated flavonoid-like compounds through color reactions and TLC profiles. The nanogel was prepared from a nanoemulsion containing Tween 80, PEG 400, olive oil, and distilled water, then incorporated into a carbopol-based gel matrix with propylene glycol, triethanolamine, and nipagin. The mixture was dried in an oven at 50 °C and stored in a desiccator for 24 hours to form a stable patch. Physical evaluation showed a pH of 6.0–6.7, patch thickness of 0.106–0.113 mm, and nanoparticle sizes of 74.46, 108.2, and 118.7 nm for Formulas 1–3, respectively. In vivo testing used diabetic rats divided into five groups: positive control (iodine plaster), negative control (untreated), and Formulas 1–3 with extract ratios of 3:1, 1:1, and 1:3. Formula 3, with a particle size of 118.7 nm, achieved complete wound closure by day 7. These findings highlight the potential of this nanogel-based transdermal system as a promising foundation for developing effective topical therapies for diabetic gangrene wounds
Co-Authors Ahmad Fuad Masduqi Ahmad Fuad Masduqi Alliva Ro’ain Abbiyu Imanita Christina Astutiningsih Darumas P, Athika Dewi Fitriani Puspitasari Erlita Verdia Mutiara Erwin Indriyanti Erwin Indriyanti Fania Putri Wiba Maharani Handayani, Tien Wahyu Hanun Aishy Marwa Indah Sulistyarini Indriyanti, Erwin Joni Tandi, Joni Mariyani Mariyani Marzuki, Sitti Aisyah Muthmainnah A.R, Sririzqi Nadia, Lailatun Nadine Ayu Syaima Gustaf Prins Nafi’ah, Rohmatun Polibaon, Tresya J. Riani, Elfa Rifda Naufa Lina Sekarwati, Anisa Sene, Ikhsan Hi. Amir Sri Wahyuni Wulandari, Anggita Dipika