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Analysis Pork Contamination in Beef Meatballs through Polymerase Chain Reaction in Palu City Tresya J. Polibaon; Mariyani; Joni Tandi; Tien Wahyu Handayani; Rahmawati Salsa Dinurrosifa; Ikhsan Hi. Amir Sene
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.9006

Abstract

The need for halal products is essential, particularly among various processed beef products like meatballs, which are popular among nearly the entire community. These products are susceptible to issues of meat counterfeiting for greater economic gain. This study aims to determine the smallest DNA concentration that can still be detected and to assess whether there is any pork contamination in meatballs circulating in Palu City using Polymerase Chain Reaction (PCR). This test uses four DNA samples obtained from fresh meat to test the specificity of the primer: wild boar, rat, and goat DNA samples as comparisons, and pig DNA as a positive control. The presence of DNA bands in the agarose gel electrophoresis visualization results indicates that DNA amplification has occurred, meaning that the sample can be detected. The analysis results showed that the primer used (Cyt-b) had good specificity for detecting pork DNA, while the absolute pork DNA concentration of 0.01 ng/µL and the reference meatball DNA with a concentration of 0.01% are the smallest limits that can still be detected using PCR. Of the four meatball samples from Palu City, one sample produced a DNA band, indicating that the sample contains or is contaminated with pig DNA. This method is valid as it meets the specificity test and demonstrates a good detection limit.
Peningkatan Pemahaman Santri dan Pengelola Pesantren dalam Mengolah dan Mengonsumsi Produk Serbuk Kelor-Jahe di Pondok Pesantren As-Salamah Betahwalang Kabupaten Demak Anggita Dipika Wulandari; Rifda Naufa Lina; Matheus Prayoga Claus; Rahmawati salsa dinurrosifa; Laelatul Husniyah; Rizki Annisa Ramadhani; Setya Wahono; Aldo Octavian Ramadhan Saputra
Cakrawala: Jurnal Pengabdian Masyarakat Global Vol. 5 No. 3 (2026): Agustus: Cakrawala: Jurnal Pengabdian Masyarakat Global
Publisher : Universitas 45 Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30640/cakrawala.v5i3.7865

Abstract

Islamic boarding schools are educational environments characterized by communal living, presenting unique challenges for health maintenance. Factors such as high-density living, shared habits, and limited understanding of nutrition and the use of herbal ingredients can impact efforts to maintain the health of the students. Moringa oleifera and Zingiber officinale are local ingredients with potential for use in food diversification and herbal beverages. This community service activity aimed to enhance the understanding and skills of the students and administrators at the As-Salamah Betahwalang Islamic Boarding School regarding the utilization of Moringa and ginger by processing them into instant powder products. The activity employed an educational and participatory approach, encompassing education on balanced nutrition and immunity, an introduction to the benefits of Moringa and ginger, plant cultivation practices, demonstrations and training on instant powder production and packaging, and evaluation via pre- and post-tests. Participants actively engaged in discussions, demonstrations, processing practices, and cultivation activities. A Moringa-ginger instant powder product was successfully produced through a process involving ingredient preparation, boiling Moringa, processing ginger, filtering, heating, adding sugar, stirring until powder formed, and packaging. The activity also established Moringa and ginger cultivation practices as a strategy to ensure a sustainable supply of raw materials. This program provided the partners with a foundation of knowledge and skills for utilizing local herbal ingredients to create practical products with the potential for independent and sustainable development.