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All Journal Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) Jurnal Penelitian Pendidikan IPA (JPPIPA) Al-Kimia JKPK (Jurnal Kimia dan Pendidikan Kimia) AMALIAH: JURNAL PENGABDIAN KEPADA MASYARAKAT Media Farmasi Indonesia Sewagati: Jurnal Pengabdian Masyarakat Indonesia Jurnal Ilmiah Sains Sabangka Abdimas Jurnal Pengabdian Masyarakat Sabangka Parapemikir Jurnal Ilmiah Farmasi
Rahmawati Salsa Dinurrosifa
Universitas Negeri Semarang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Other

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Analysis Pork Contamination in Beef Meatballs through Polymerase Chain Reaction in Palu City Polibaon, Tresya J.; Mariyani; Tandi, Joni; Handayani, Tien Wahyu; Dinurrosifa, Rahmawati Salsa; Sene, Ikhsan Hi. Amir
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.9006

Abstract

The need for halal products is essential, particularly among various processed beef products like meatballs, which are popular among nearly the entire community. These products are susceptible to issues of meat counterfeiting for greater economic gain. This study aims to determine the smallest DNA concentration that can still be detected and to assess whether there is any pork contamination in meatballs circulating in Palu City using Polymerase Chain Reaction (PCR). This test uses four DNA samples obtained from fresh meat to test the specificity of the primer: wild boar, rat, and goat DNA samples as comparisons, and pig DNA as a positive control. The presence of DNA bands in the agarose gel electrophoresis visualization results indicates that DNA amplification has occurred, meaning that the sample can be detected. The analysis results showed that the primer used (Cyt-b) had good specificity for detecting pork DNA, while the absolute pork DNA concentration of 0.01 ng/µL and the reference meatball DNA with a concentration of 0.01% are the smallest limits that can still be detected using PCR. Of the four meatball samples from Palu City, one sample produced a DNA band, indicating that the sample contains or is contaminated with pig DNA. This method is valid as it meets the specificity test and demonstrates a good detection limit.
Co-Authors Ahmad Fuad Masduqi Ahmad Fuad Masduqi Christina Astutiningsih Darumas P, Athika Dewi Fitriani Puspitasari Erlita Verdia Mutiara Erwin Indriyanti Erwin Indriyanti Handayani, Tien Wahyu Indah Sulistyarini Indriyanti, Erwin Joni Tandi, Joni Mariyani Mariyani Marzuki, Sitti Aisyah Muthmainnah A.R, Sririzqi Nadia, Lailatun Nafi’ah, Rohmatun Polibaon, Tresya J. Riani, Elfa Rifda Naufa Lina Sekarwati, Anisa Sene, Ikhsan Hi. Amir Sri Wahyuni Wulandari, Anggita Dipika