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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Microbiology Indonesia agriTECH JURNAL MANAJEMEN TRANSPORTASI & LOGISTIK Industria: Jurnal Teknologi dan Manajemen Agroindustri Animal Production : Indonesian Journal of Animal Production Jurnal Gizi Klinik Indonesia Indonesian Food and Nutrition Progress Indonesian Food Science and TechnologyJournal Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Mutu Pangan : Indonesian Journal of Food Quality Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Manajemen Transportasi & Logistik (JMTRANSLOG)
Winiati Pudji Rahayu
Department Of Food Science And Technology, Faculty Of Agricultural Technology, Bogor Agricultural University, Campus IPB Dramaga, PO Box 220 Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Public Health Transportation Veterinary Other

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Review: Prevention and Reduction of Mycotoxin by Antagonistic Microorganism Winiati Pudji Rahayu
Indonesian Food and Nutrition Progress Vol 15, No 2 (2018)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (805.152 KB) | DOI: 10.22146/ifnp.39607

Abstract

Mycotoxin is widely known as one cause of foodborne disease, produced by toxigenic fungi. Any country should be aware about this high risk potency by knowing the mycotoxin, affected commodities, fungal sources, and toxicity effect to human or animal. Controlling mycotoxin could be done by physic, chemical, and biological methods. The microbial characteristic used for biological agent should be evaluated including the inability to produce toxic substance, tendency to multiply, colonize, survive, safety, and applicability to the environment. Studies related to mycotoxin biocontrol by using antagonistic microorganism can be focused on (1) the effect to the mycotoxin, (2) the growth of microorganism, or (3) the application to food both raw material and processed products. Consideration to combine more than one species of microorganism instead of a single species also has been taken to achieve more effective result.  For example, S. cerevisiae has been used together with LAB to control certain mycotoxin. Further studies are needed to develop the possibility of other biological agents and the effect of their application, which in the next have the potency as manufacturing products.
Patulin-producing mold, toxicological, biosynthesis, and molecular detection of patulin Ratih Paramastuti; Winiati Pudji Rahayu; Siti Nurjanah
Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Vol 4, No 2 (2021)
Publisher : Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.afssaae.2021.004.02.3

Abstract

Mycotoxin is one of the food safety problems that raise concern because of its negative health impact and significant economic losses. Patulin is the most common mycotoxin found in fruits and its products like apple, orange, grape, pear, etc. There are 167 species reported to produce patulin; Penicillium expansum is the major patulin producer among those fungi species. Patulin is not classifiable as to its carcinogenicity to humans, but some studies showed the toxicities of patulin.  Patulin has a broad spectrum of toxicity such as acute toxicity, sub-acute toxicity, genotoxicity, embryotoxicity and teratogenicity, carcinogenicity, and immunotoxicity. There are several ways to detect mycotoxins, such as HPLC, TLC, ELISA, and PCR. The popular way to detect mycotoxin molecularly is PCR because of its sensitivity and specificity. To detect patulin molecularly require proper selection of target gene. Therefore, the biosynthesis of patulin, its influencing gene, and its underlying factor needs to be understood.
Evaluasi Sistem Jaminan Keamanan Pangan di Restoran Multicabang PT XYZ Area Jakarta Katarina Maharani; Winiati Pudji Rahayu; Siti Nurjanah
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 10 No. 1 (2023): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jmpi.2023.10.1.54

Abstract

Improper restaurant management causes potential food safety hazards which can lead to decline in public health. This study aims to evaluate food safety system at restaurant multisite PT XYZ area Jakarta to determine the knowledge of managers and knowledge, attitudes and practices of food handler about food safety, analyze level correlation between knowledge food handlers with attitudes and practices, correlate food safety knowledge restaurant managers with E. coli contamination and also observed general food safety implementation in restaurant. Data collection was by filling out questionnaires by 136 food handlers and 44 restaurant manager and observation of food safety in 44 restaurants. The results showed that restaurant managers had good knowledge of food safety and 63.6% had attended food safety training. Food handlers had good knowledge, attitudes, and practices (77.21, 84.56, 80.15%). Statistical analysis did not indicate a significant correlation between knowledge and attitudes. However, it was found that there is correlation between knowledge with practice and attitudes with practice. Observation result of restaurant food safety implementation showed that 93.8% had a good implementation. The knowledge of restaurant managers correlated positively with the implementation of restaurant food safety practices, but did not correlate with the presence of E.coli. The presence of E.coli did not indicate corelation with restaurant observation value. Testing E.coli at 44 restaurants showed that 9 of the salad samples and 3 of the sushi samples were detected by E.coli, while the ice coffee milk did not detect E. coli.
Pemahaman dan Penerimaan Label Gizi Front of Pack Produk Snack oleh Siswa SMA di Depok Iftita Rakhma Ikrima; Puspo Edi Giriwono; Winiati Pudji Rahayu
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 10 No. 1 (2023): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jmpi.2023.10.1.42

Abstract

Labels play a crucial role in providing essential information that helps consumers choose their food products. Despite this, the comprehension and acceptance of nutrition labels remain low. The objective of this study was to compare the understanding and acceptance of nutrition labels with and without Front of Pack (FoP) in adolescents aged between 15 and 18 years. The FoP nutrition label is a labeling system that simplifies nutritional information and displays it on the front of food packaging. It includes three types: daily intake guide (DIG), multi-traffic light (MTL), and health star rating (HSR). The study targeted high school students in Depok, selected through stratified sampling based on school accreditation. Only 11.1% of respondents reported reading nutrition labels. The results of the Chi-square test indicated a significant difference (p <0.05) in the use of nutrition labels based on exposure to nutrition, nutritional knowledge, and label reading. ANOVA test results showed significant differences in comprehension and acceptance of the four labels. The HSR label was the most comprehensible (63.7%), followed by DIG (58.7%), nutrition fact panel/NFP (51.1%), and MTL (45.7%). Additionally, respondents rated the HSR label as the most preferred, attractive, and easiest to understand.
Ekstrak Bawang Dayak Penghambat Pertumbuhan Escherichia coli dan Staphylococcus aureus pada Daging Ayam Taufikkilah Romadhon; Winiati Pudji Rahayu; Harsi Dewantari Kusumanigrum
Jurnal Ilmu Pertanian Indonesia Vol. 28 No. 3 (2023): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.28.3.504

Abstract

Microbial contamination on chicken meat can be inhibited by applying natural antimicrobials. Dayak onions (Eleutherine palmifolia (L.) Merr.) are reported to inhibit the growth of Escherichia coli and Staphylococcus aureus. This study aims to confirm and apply dayak onion extract (EBD) as an antimicrobial in fresh chicken meat. The observation parameters were the microbiological quality and physical properties of chicken meat after inoculation with E. coli and S. aureus and dipped in EBD solution with concentrations of 0, 3.0, 9.0, and 15.0 mg/mL for E. coli, and 0, 2.5, 7.5, and 12.5 mg/mL for S. aureus. The results showed that EBD-70% ethanol showed better bacterial inhibition than EBD-96% ethanol. Inhibition for both microbes can be achieved by concentrations of 3.0 and 2.5 mg/mL EBD, as visually observed in liquid media. Concentrations of 15.0 and 12.5 mg/mL showed significant results in reducing the total microbes of both types in chicken meat after 12 hours of room temperature storage (28±2°C) and 9 days of refrigerator storage (5±1°C). A combined application of 15.0 and 12.5 mg/mL EBD and refrigerator temperature can be recommended to reduce total E. coli and S. aureus and maintain the Aw and pH of the chicken meat. Keywords: antimicrobial, chicken meat, dayak onion extract, Escherichia coli, Staphylococcus aureus
Keamanan Mikrobiologis Air Minum Isi Ulang dan Perubahannya Selama Penyimpanan Tasya Fitri Yunada; Winiati Pudji Rahayu; Dian Herawati
Jurnal Ilmu Pertanian Indonesia Vol. 28 No. 4 (2023): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.28.4.581

Abstract

Indonesians use refilled drinking water which can be obtained at depots because of practical and affordable reasons. Low levels of sanitation and hygiene and storage of refilled drinking water for an extended period can lead to the possibility of the growth of indicator and pathogenic microorganisms such as Coliform and Escherichia coli (E. coli). This study aims to evaluate the application of drinking water refilling stations (DWRS) sanitary hygiene, microbiological quality and safety of refilled drinking water and its changes during storage. Samples of refilled drinking water were taken from two DWRSs for each region in five areas of DKI Jakarta including West, East, South, North, and Central Jakarta. The treatments in this study were refilled drinking water storage in gallons of closed and open-closed conditions at room temperature (28 + 2°C) for 0, 7, and 14 days. The results of observations showed that 30% (n=30) of depots complied with hygiene sanitation standards according to Permenkes RI No. 43 of 2014 (>70%). The analysis results showed that refilled drinking water at 0 days of storage, 100% met the TPC (5 Log CFU/mL), 20% met the total Coliform standard (0 Log CFU/mL), and 80% met the E. coli standard (0 Log CFU/mL) according to Permenkes RI No. 492 of 2010. Storage times of 0, 7, and 14 days of refilled drinking water had a significant effect on TPC and total Coliform (P < 0.05), but had no significant effect on E. coli (P > 0.05) in gallons with closed and open-closed conditions. Keywords: Coliform, DWRS, E. coli, refilled drinking water
Relevansi Budaya Keamanan Pangan dengan Implementasi Teknologi Industri 4.0 di Industri Pangan Indonesia Bangun Raharjo; Winiati Pudji Rahayu; Dase Hunaefi
Jurnal Teknologi dan Industri Pangan Vol. 34 No. 2 (2023): Jurnal Teknologi dan Industri Pangan
Publisher : Perhimpunan Ahli Teknologi Pangan Indonesia bekerjasama dengan Departemen Ilmu dan Teknologi Pangan, IPB University Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2023.34.2.152

Abstract

The application of food safety culture (FSC) which is influenced by human behavior with the development of industrial technology (IT) 4.0 with less human resources provides a challenge to understand the relationship between them. The research objective was to provide quantitative data and suggestions for improving the implementation of the FSC dimensions in synergy with the progress of IT 4.0. This research involved 35 participants from 18 local companies and 17 multinational food companies (MFC/PMA) for the survey and invited 7 selected participants from both of them to join the FGD. The FSC survey showed that the gap organizational maturity in FSC implementation between local companies (2.93) and PMA (3.62) in Indonesia was 0.7 (world best practice 4.0 – 5.0). The three main benefits and opportunities for implementing IT 4.0 were effectiveness and efficiency, safe and quality products, and early detection to prevent non-conformities or product recalls. There were three main IT 4.0 application areas, namely production, quality, and engineering. The three main forms of IT 4.0 implementation were advanced robotics, big data, and internet of things (IoT). The FGD results showed the relevance between FSC and IT 4.0. Activities in IT 4.0 helped the food industry to manage food safety and quality management system better, because technical problems that previously took time and thought can be implemented effectively and efficiently. Data analysis can be carried out more in-depth, actual, and accurate. Further research is recommended to see the strength of the relationship between FSC and IT 4.0 parameters to determine critical areas with quantitative research methods and advanced statistical data processing.
Deteksi dan Identifikasi Molekuler Kapang Berpotensi sebagai Penghasil Patulin pada Buah Tropis Ratih Paramastuti; Winiati Pudji Rahayu; Siti Nurjanah
Jurnal Teknologi dan Industri Pangan Vol. 34 No. 2 (2023): Jurnal Teknologi dan Industri Pangan
Publisher : Perhimpunan Ahli Teknologi Pangan Indonesia bekerjasama dengan Departemen Ilmu dan Teknologi Pangan, IPB University Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2023.34.2.127

Abstract

Production and export of tropical fruits in Indonesia have an increasing trend every year. The important factor in the production and export of fruits is food safety. One of the contaminants that may be found in fruits is mycotoxin, especially patulin. Patulin mainly found in fruits such as apple, orange, grape, and pear. This study aimed to detect molds from tropical fruits and to identify potentially patulin-producing molds. Detection of potentially patulin-producing molds obtained from tropical fruits was carried out using the isoepoxydon dehydrogenase (idh) gene. Species identification was carried out using internal transcribed spacer (ITS) region. The mold isolate sequences of ITS rDNA region were analyzed for their homology using both BLAST search and phylogenetic tree. A total of 26 molds were isolated from four types of fruit (malang apple, ambon banana, medan orange, and avocado) obtained from five different places in Bogor including fruit stalls, traditional markets, and supermarkets. The positive results of idh gene were mold isolates that have an amplicon at 620 bp. The result showed that 5 of 26 (19,2%) isolates were positive for idh gene, namely A11, A34, A43, A51 and B23. The positive isolates for idh gene were identified as Aspergillus aculeatus, Aspergillus niger, Cladosporium anthropophilum, Cladosporium tenuissimum, and Talaromyces verruculosus.
Rheological Characterization of Sugar Palm Fruits (Arenga pinnata) at Different Maturity Levels and Concentrations Yermia, Yermia; Pudji Rahayu, Winiati; Edhi Suyatma, Nugraha; Muhandri, Tjahja; Hari Purnomo, Eko
Indonesian Food Science and Technology Journal Vol. 8 No. 1 (2024): Volume 8. Number 1, December 2024 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v8i1.37940

Abstract

Aren is a versatile crop with significant economic potential in tropical regions, particularly Southeast Asia. While young fruits are commonly processed into kolang-kaling, the industrial potential of both young and matured fruits, especially about their rheological and structural properties for edible packaging, still needs to be explored. This study investigates palm fruits' rheological and structural properties at different maturity stages and evaluates their potential for industrial applications, specifically in developing edible films. Rheometer testing revealed that young fruits exhibit pseudoplastic flow behavior (n < 1), whereas matured fruits display Newtonian flow behavior (n = 1). The consistency index (K) increased as concentration. Dynamic rheological measurement showed that young fruits experienced a decrease in LVR and matured fruits showed an increase at higher concentration. The storage modulus (G') of matured fruits was higher than that of young fruits, indicating better mechanical stability in the former Both young and matured fruit had a loss factor < 1 and G' greater than G", indicating elastic behavior predominance. X-ray diffraction (XRD) analysis showed that matured fruits have a crystalline structure, while young fruits are semi-crystalline. The crystalline properties of matured fruits make them suitable for applications requiring mechanical stability, such as edible film production. In contrast, the semi-crystalline nature of young fruits is better suited for flexible packaging applications. These findings open new opportunities for utilizing palm fruit as an innovative base material in developing eco-friendly edible packaging for the food industry.  
Homogenitas, Stabilitas, dan Konfirmasi Molekuler Biakan Kering Beku Campuran Escherichia coli dan Salmonella Typhimurium Nurjanah, Siti; Agesti, Puja; Rahayu, Winiati Pudji; Hasanah, Uswatun; Nanda, Riska Dwi
Jurnal Teknologi dan Industri Pangan Vol. 35 No. 2 (2024): Jurnal Teknologi dan Industri Pangan
Publisher : Perhimpunan Ahli Teknologi Pangan Indonesia bekerjasama dengan Departemen Ilmu dan Teknologi Pangan, IPB University Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2024.35.2.210

Abstract

Food product testing laboratories play a prominent role in ensuring safety and quality of products manufactured by food industries; therefore, they shall comply with standards of ISO/IEC 17025:2017. Although a proficiency test for microbiological parameter is required to evaluate testing laboratories' performance in considering food product quality and safety, the existence of proficiency test organizations in Indonesia is currently still limited. This study was conducted to determine the appropriate types of cryoprotectant, evaluate the degree of homogeneity and stability of the freeze-dried cultures of Escherichia coli and Salmonella Typhimurium for preparation of proficiency testing item, as well as molecular confirmation of E. coli and S. Typhimurium bacteria using PCR. The freeze-dried cultures were prepared for the purpose of producing the proficiency testing object. Three main stages were conducted, namely cryoprotectant effectiveness test, homogenization test and stability test at 4 °C storage temperature for four different times (0, 7, 14, 65 days), as well as molecular confirmation test. The best cryoprotectant treatment was using 10% trehalose with the viabie E. coli and S. Typhimurium respectively 5.58±0.25 and 5.31±0.09 log CFU/mL. Freeze-dried culture samples of E. coli and S. Typhimurium was declared homogeneous and stable based on ISO 13528:2015 calculations. E. coli bacteria were confirmed using standard PCR with 16S rRNA primer, while S. Typhimurium was confirmed using rt-PCR with invA primer.
Co-Authors Afrilia Sandra Ramadhani Agesti, Puja Amiroh Amiroh Amiroh Amiroh, Amiroh Andro Frando Situmorang Anjani Anggitasari Arie Norman Riandi Bangun Raharjo Betty Sri Laksmi Jenie Budi Nurtama C Hanny Wijaya Dase Hunaefi Dede Robiatul Adawiyah Dharmawan, I P G Arya Dian Herawati Dr. Adolf J.N. Parhusip Dwi Rahayu Dyah Sista Raharjanti Eko Hari Purnomo Ervizal AMZU Feri Kusnandar Galih Nugroho Gerardus YosuaYosua Gita, Sophia Ekaristi Dharma Hanifah Nuryani Jenie Harsi D. Kusumaningrum I P G Arya Dharmawan Iftita Rakhma Ikrima Jian Septian Katarina Maharani KIKI NURTJAHJA Lilis Nuraida MADE ASTAWAN Meike Meilan Lisangan Nanda, Riska Dwi Ni Made Vina Citanirmala Nugraha Edhi Suyatma Nugroho, Galih Nuri Andarwulan Nurwitri, Caecillia Chrismi Okky Setyawati Dharmaputra Okky Setyawati Dharmaputra Oryssa Sathalica Pradianti Palupi, Nurheni Sri Pipi Puspita Sari Puspa Mega Hayati Puspo Edi Giriwono Putri, Permata Adinda Qonitatin Wafiyah Ratih Dewanti -Hariyadi Ratih Paramastuti Rizal Syarie Rizal Syarief Ruki Fanaike Santi Ambarwati SEDARNAWATI YASNI Septian, Jian Siti Nurjanah Siti Nurjanah Siti Nurulfalah Situmorang, Andro Frando Sophia Ekaristi Dharma Gita Tasya Fitri Yunada Taufikkilah Romadhon Tita Rialita Tjahja Muhandri Triana Setiawardani, Triana Uswatun Hasanah Wibisono Adhi Wibisono Adhi, Wibisono Wibisono Wibisono Wibisono Wibisono Widiati Purnawita WIDYA EKA PRAYITNO Wisnu Broto Wiwit Arif Wijaya Yanti Ratnasari yermia, yermia yermia Yosua, Gerardus