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All Journal Biopropal Industri BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Teknologi Indonesia Quality : Jurnal Kesehatan Makara Journal of Science
NANIK RAHMANI
Research Center for Biotechnology,Indonesia Institute of Sciences, Jl Raya Bogor KM.46 Cibinong, Bogor, 16911, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Industrial & Manufacturing Engineering Nursing

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Enzymatic Hydrolysis of Mannan from Konjac (Amorphophallus sp.) Using Mannanase from Streptomyces lipmanii to Produce Manno-oligosaccharides Sasongko, Ashadi; Yopi,; Rahmani, Nanik; Lisdiyanti, Puspita; Saepudin, Endang
Makara Journal of Science Vol. 19, No. 3
Publisher : UI Scholars Hub

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Abstract

Mannan is an abundant polysaccharide that can be found in konjac (Amorphophallus sp.). Mannan can be enzymatically hydrolyzed using mannanase to produce manno-oligosaccharides which can be used as a prebiotic. The aims of this research are to determine the production time of mannanase from Streptomyces lipmanii, perform enzyme characterization, optimize the hydrolysis time, and characterize the hydrolysis product. A qualitative assay using the indicator Congo red showed that S. lipmanii generated a clear zone, indicating that S. lipmanii produced mannanase in konjac medium and possessed mannanolytic activity. Enzyme activity was determined through reducing sugar measurement using the dinitrosalycylic acid method, and optimum enzyme production was achieved at the second day of culture. Characterization of the enzyme showed that hydrolysis was optimum at pH 7 and at a temperature of 50 oC. The reducing sugar content was increased by an increasing the hydrolysis time, and reached an optimum time at 2 h. The degree of polymerization value of three was achieved after 2 h hydrolysis of mannan from konjac, indicating the formation of oligosaccharides. Analysis by thin layer chromatography using butanol, acetic acid, and water in a ratio of 2:1:1 as eluent showed the presence of compounds with a retention time between those of mannose and mannotetrose. Confirmation was also performed by HPLC, based on the retention time.
BIOPROSPECT OF BACTERIAL FIBRINOLYTIC PROTEASE FROM BEKASAM OF LONGTAIL TUNA AS ANTITHROMBOTIC AGENT: LITERATURE REVIEW AND BIBLIOGRAPHY STUDY Trianes, Juwy; Ethica, Stalis N.; Afriansyah, M. Ardi; Darmawati, Sri; Rahmani, Nanik; Zilda, Dewi S.
Quality : Jurnal Kesehatan Vol. 18 No. 2 (2024): Quality : Jurnal Kesehatan
Publisher : Politeknik Kesehatan Kemenkes RI Jakarta I

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36082/qjk.v18i2.2016

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Introduction:Cardiovascular disease (CVD) is the leading cause of death worldwide, with thrombosis being a significant risk factor. Fibrinolytic proteases have potential as antithrombotic agents and could be developed into CVD drugs. Indonesia's traditional fermented products, such as fish Bekasam, are rich in microorganisms, including fibrinolytic protease-producing bacteria, yet their potential for CVD treatment is underexplored. Fish paste in Indonesia serves as a protein source and a reservoir of bacterial diversity, which could aid in discovering new antithrombotic agents. Objective: This literature review examines research trends over the past decade on fibrinolytic protease-producing bacteria in traditional fermented products, with a focus on Bekasam made from Longtail Tuna. This fermented food is an alternative source for obtaining bacteria with antithrombotic properties. Methods: Data for this review were sourced from Google Scholar, PubMed, and the dimension.ai database, using the keyword "bacterial fibrinolytic protease" from 2015 to 2024. Visualization of global research trends was performed using VOS viewer software. Results: The review found a scarcity of studies on fibrinolytic proteases from Bekasam bacteria. Lactic acid bacteria involved in Bekasam fermentation possess proteolytic enzymes that degrade fish protein into peptides and amino acids, potentially offering antithrombotic properties. This suggests natural protease sources from traditional fermented foods have significant biomedical potential. Research on fibrinolytic protease-producing bacteria from Bekasam in Indonesia is limited and requires further development. Exploring these local fermented products could yield innovative sources for thrombosis treatment
Co-Authors - Yopi -, Yopi - ADE ANDRIANI Afriansyah, M. Ardi Ahmad Thontowi Anggraini, Yufi Sara Anja Meryandini Ashadi Sasongko Dewi, Fitria Endang Saepudin, Endang Ethica, Stalis N. Nuryati Nuryati Palupi, Nurheni Sri PUSPITA LISDIYANTI Rahmasari, Dianti Rahmasari, Dianti Rohmattusolihat Rohmattusolihat, Rohmattusolihat Ruth Melliawati Sari, Yana Nurita Sari, Yana Nurita Sri Darmawati Sri Hartati Sri Pujiyanto Trianes, Juwy Wijanarka Wijanarka YOPI YOPI Yopi, Zilda, Dewi S.