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INDRA RAHMAWATI
Center for Biotechnology , BPPT, Puspiptek-Serpong, Tangerang Selatan, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Case Study of Pseudomonas sp. in Giant Gourami (Osphronemus goramy) via Anatomical Pathology, Histopathology, and Microbiological Examinations, and Tetracycline Sensitivity Testing Rahmaniar, Reina Puspita; Rahmawati, Indra; Nussa, Olan Rahayu Puji Astuti; Firmansyah, Muhammad Irwan
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): in Progress
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12956

Abstract

Pseudomonas sp. is an opportunistic bacterial pathogen causing systemic infections in cultured fish, resulting in economic losses and increasing concerns regarding antimicrobial resistance. However, comprehensive pathological and microbiological confirmation remains limited. This study aimed to evaluate Pseudomonas sp. infection in giant gourami (Osphronemus goramy) through pathological examination, bacterial identification, and antibiotic susceptibility testing. A descriptive case study was conducted on one naturally infected fish (code F-11). Clinical observation, necropsy, histopathological examination of the liver and gills using hematoxylin-eosin staining, bacterial isolation on MacConkey agar, Gram staining, biochemical characterization, and Kirby-Bauer disk diffusion testing with 30 μg tetracycline were performed. Data were analyzed descriptively by integrating pathological and microbiological findings. Gross examination revealed ecchymotic hemorrhages in the gills, labyrinth organ, and liver, hepatic nodules, and jejunal petechiae. Histopathology demonstrated congestion, inflammatory cell infiltration, hemorrhage, focal hepatic necrosis, epithelial desquamation, and lamellar necrosis in the gills. The isolate produced transparent non-lactose-fermenting colonies, consisted of Gram-negative rods, and exhibited biochemical characteristics consistent with Pseudomonas sp. The isolate remained susceptible to tetracycline, producing a 28-mm inhibition zone. These findings confirm Pseudomonas sp. infection and highlight the importance of integrated pathological and microbiological diagnosis to support appropriate antimicrobial therapy and antimicrobial resistance surveillance in aquaculture.
Co-Authors Bagus Uda Palgunadi, Bagus Uda Deo, Amelia Alfatiani Putri de Dian Masyitha Faisal Mustafa Firdausi, Nida Khofiya Firmansyah, Muhammad Irwan Ginting, Eykel Arief Fortuna Hamny Sofyan Kusuma Wardhani, Lailia Dwi M. Jalaluddin, M. NURUL HIDAYAH Nussa, Olan Rahayu Puji Astuti Puji Astuti Nussa, Olan Rahayu Putri, Agnes Salsabila Rahayu Puji Astuti Nussa, Olan Rahmaniar, Reina Puspita Ritonga, Mudhita Zikkrullah Rochiman Sasmita Roeswandono, Roeswandono Siti Gusti Ningrum, Siti Gusti Sri Wahyuni Wardhani, Lailia Dwi Kusuma Wibisono, Freshinta Jellia