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All Journal ad-Dawaa : Journal of Pharmaceutical Sciences Jurnal farmasi UIN Alauddin Makassar
Haeria Haeria
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Profil Indeks Pengembangan Ikatan-Silang Gelatin-Kitosan Nursalam Hamzah; Muhammad Fadhlurrahman; Surya Ningsi; Haeria Haeria
Ad-Dawaa: Journal of Pharmaceutical Sciences Vol 2 No 2 (2019)
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.624 KB) | DOI: 10.24252/djps.v2i2.12147

Abstract

Gelatin ikan menjadi sumber alternatif gelatin halal, tetapi memiliki sifat mekanik dan kekuatan gel yang rendah. Ikat silang telah banyak digunakan pada modifikasi material, termasuk untuk membentuk kompleks gelatin-kitosan. Ikat silang dapat meningkatkan kemampuan sifat mekanik dan kekuatan gel. Fokus penelitian ini adalah mengamati karakteristik indeks pengembangan kompleks gelatin-kitosan yang diikat silang menggunakan glutaraldehid dan sukrosa teroksidasi dengan variasi konsentrasi. Penelitian dimulai dengan ekstraksi gelatin dari sisik ikan Bandeng menggunakan metode basa-asam. Gelatin sisik ikan Bandeng dan gelatin komersil kemudian digunakan sebagai sampel. Larutan gelatin, kitosan dan pengikat silang dicampurkan dan diinkubasi pada suhu 40oC selama selama 24 jam. Campuran kemudian dikeringkan pemanasan suhu 70oC sampai sampel kering. Sampel kemudian diuji indeks pengembangan dengan direndam dalam air selama 12 jam, volume pengembangan diamati tiap jam. Hasil yang diperoleh menunjukkan bahwa kompleks gelatin-kitosan dapat diikat metode ikat silang dengan glutaraldehid dan sukrosa teroksidasi yang ditunjukkan dengan perubahan nilai indeks pengembangan. Sukrosa teroksidasi dengan perbandingan sukrosa dan natrium periodat 1:3 menunjukkan nilai indeks pengembangan paling baik.
Antiproliferation potential of Botto-botto (Chromolaena odorata L.) leaves methanol extract fraction against HeLa Cell Line Muhammad Rusdi; Haeria Haeria; Nursalam Hamzah; Afrisusnawati Rauf; Faradibha Amriani
Ad-Dawaa: Journal of Pharmaceutical Sciences Vol 3 No 2 (2020)
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/djps.v3i2.14051

Abstract

Botto-botto (Chromolaena odorata L.) was one of plants that its leaves used empirically in South Sulawesi society as wound and anti-inflammatory drugs. This study aimed to determine the potential of Botto-botto toward cancer cells (HeLa Cells) and normal cells (Vero cells) using the MTT assay method (Microculture Tetrazolium Salt Method). Ethyl acetate extract from methanol extract being fractionated by vacuum liquid chromatography method by a different polarity level of solvent. Cytotoxic effect test was using MTT method to cancer cells (HeLa), and normal cells (Vero) with the percent parameter of cell growth inhibition. Selectivity index was determined from percentage of cancer cell growth inhibition ratio of cancer cells to normal cells. The results showed that A, B, C, D, E, F, and G fractions had a selectivity index on HeLa cells at 0.845, 3.387, 2.004, 1.508, 1.625, 1.284, 1.299. This result proved that B fraction of methanol extract of Botto-botto leaves had a potential to be developed as chemotherapy agents in increasing the effectiveness treatment of cervix cancer.
Co-Authors Afrisusnawati Rauf Afrisusnawati Rauf Alfiana Dwi Puspita Asrul Ismail Dina Dhaifina Anas Faradibha Amriani Fitriani Fajri Ahmad Hadiq, Shabran Hardiyanti Eka Putri Isriany Ismail Isriany Ismail Kamsia Dg Paewa M. Rusdi Muhammad Fadhlurrahman Mukhriani, Mukhriani Nur Syamsi Dhuha Nur Syamsi Dhuha Nur Syamsi Dhuha Nurfitra Fadiyah Nursalam Hamzah Nursalam Hamzah Nursalam Hamzah Nursyamsi Dhuha Nurul Muhlisa Mus Risnawati Habra Surya Ningsi Syamsuri Syakri Wa Sinta