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All Journal HAYATI Journal of Biosciences CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Kimia (Journal of Chemistry) Buletin Udayana Mengabdi Jurnal Veteriner Ecotrophic, Journal of Environmental Science Indonesian Journal of Cancer Jurnal Media Sains Journal of Health Sciences and Medicine INTERNATIONAL JOURNAL OF APPLIED CHEMISTRY RESEARCH Indonesian Journal of Cancer
WIWIK SUSANAH RITA
Chemistry Department, Faculty Of Mathematic And Natural Sciences Universitas Udayana Badung, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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AKTIVITAS ANTIBAKTERI MINYAK ATSIRI RIMPANG JERINGAU (Acorus calamus Linn.) TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus Wiwik Susanah Rita; I Wayan Suirta; Putu Prisanti Putri Utami
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 5 No 2 (2017)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Isolasi minyak atsiri dari rimpang jeringau (Acorus calamus Linn) dan uji aktivitas antibakteri terhadap bakteri Escherichia coli (E. coli) dan Staphylococcus aureus (S. aureus) telah dilakukan. Ekstraksi minyak atsiri dilakukan dengan metode destilasi uap, uji aktivitas antibakteri dilakukan dengan metode sumur difusi, dan identifikasi senyawa dilakukan dengan kromatografi gas-spektroskopi massa (KG-SM). Rendemen minyak yang dihasilkan sebesar 0,1653% (b/b) dengan berat jenis 1,066 g/mL. Minyak atsiri dengan konsentrasi 10% (v/v) menunjukkan aktivitas penghambatan yang kuat terhadap pertumbuhan E. coli dan S. aureus dengan diameter hambat masing-masing sebesar 11,33 mm dan 13,57 mm. Nilai konsentrasi hambat minimum (KHM) minyak atsiri terhadap E. coli dan S. aureus masing-masing sebesar 4 dan 0,4% dengan zona hambat berturut-turut 6,67 dan 8,83 mm. Kandungan senyawa utama dalam minyak atsiri rimpang jeringau adalah euasaron dan asaron yang telah diketahui memliki aktivitas sebagai antibakteri. Kata kunci: Minyak atsiri, Acorus calamus Linn, Antibakteri, Escherichia coli, Staphylococcus aureus ABSTRACT: Isolation of essential oils from rhizome of Jeringau (Acorus calamus Linn) and the antibacterial activity assay against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) has been performed. Essential oil extraction was done by steam distillation method, antibacterial activity test was conducted by diffusion wells, and compound identification was conducted by gas chromatography-mass spectroscopy (GC-MS). The essential oils with a concentration of 10% (v/v) showed a strong inhibitory activity against the growth of E. coli and S. aureus with diameter inhibition of 11.33 mm and 13.57 mm respectively. Minimum inhibitory concentration (MIC) of the oils against E. coli and S. aureus was 4 and 0.4% respectively with the inhibition zone of 6.67 and 8.83 mm. The main compounds of the oils were euasaron and asaron known having antibacterial activity.
AKTIVITAS ANTIBAKTERI EKSTRAK METANOL KULIT PISANG PECAH SERIBU (Musa x paradisiaca L.) TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coli Wiwik Susanah Rita; Irvansyah Habibi Resaputra; I Made Sukadana
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 8 No 2 (2020): Volume 8, Nomor 2, 2020
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Musa x paradisiaca L. merupakan salah satu jenis pisang yang berpotensi sebagai antibakteri. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri ekstrak metanol kulit buah pisang pecah seribu (Musa x paradisiaca L.) terhadap bakteri Staphylococcus aureus dan Escherichia coli serta menentukan kandungan metabolit sekundernya. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut metanol 95% pengujian aktivitas antibakteri dilakukan dengan metode sumur difusi, dan kandungan metabolit sekuder dalam ekstrak metanol dilakukan dengan uji fitokimia. Hasil uji fitokimia menunjukkan bahwa ekstrak mengandung metabolit sekunder flavonoid, fenol, alkaloid, dan triterpenoid. Hasil uji antibakteri menunjukkan bahwa Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) ekstrak metanol terhadap Staphylococcus aureus sebesar 4% dan Escherichia coli sebesar 6% dengan daya hambat masing-masing sebesar 6,83 dan 5,25 mm. ABSTRACT: Musa x paradisiaca L. is one type of banana that has potential as an antibacterial. The purpose of this study was to determine the antibacterial activity of the methanol extract of pecah seribu banana peels (Musa x paradisiaca L.) against Staphylococcus aureus and Escherichia coli and determine the content of secondary metabolites. Extraction was carried out by maceration using 95% methanol. Antibacterial activity assay was perfomed by the diffusion well method and the content of secondary metabolites in the methanol extract was carried out by phytochemical tests. The phytochemical test results show that the extract contains secondary metabolites of flavonoid, phenol, alkaloid, and triterpenoid. The antibacterial assay results revealed that the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of methanol extract against Staphylococcus aureus of 4% and Escherichia coli of 6% with inhibition of 6.83 and 5.25 mm, respectively.
AKTIVITAS ANTIJAMUR EKSTRAK n-BUTANOL DARI DAUN TREMBESI (Albizia saman (Jacq.) Merr) TERHADAP JAMUR Candida albicans DAN PENENTUAN TOTAL FLAVONOID Adi Djona Silaen; Wiwik Susanah Rita; I Made Dira Swantara
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 8 No 1 (2020): Volume 8, Nomor 1, 2020
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK. Daun trembesi (Albizia saman (Jacq) Merr) belum banyak dimanfaatkan oleh banyak orang meskipun daun trembesi dapat digunakan sebagai obat antijamur. Tujuan penelitian ini adalah untuk mengetahui aktivitas ekstrak n-butanol daun trembesi terhadap jamur Candida albicans serta mengetahui kandungan total flavonoid pada ekstrak n-butanol. Ekstraksi daun trembesi dilakukan menggunakan metode maserasi dan partisi, pengujian aktivasi antijamur dengan metode sumur difusi, dan penentuan kandungan total flavonoid dilakukan dengan Spektrofotometer UV-Vis. Maserasi 700 g serbuk daun trembesi menghasilkan 68,95 g ekstrak etanol. Hasil partisi menggunakan n-heksana, kloroform, dan n-butanol berturut-turut 34,59; 8,92; dan 4,23 g. Hasil uji antijamur terhadap C. albicans menunjukkan bahwa ekstrak n-butanol 30% memiliki aktivitas yang paling tinggi. Nilai Konsentrasi Hambat Minimum (KHM) dari ekstrak n-butanol sebesar 10% dengan diameter hambat 7 mm. ABSTRACT. Leaves of Trembesi (Albizia saman (Jacq) Merr) has not been used commonly by people eventhough the leaves can be applied as antifungal. The aims of this study were to reveal the activity n-butanol extract against Candida albicans and to determine the total content of flavonoid. Extraction of the trembesi leaves was done by maceration and partition method. The antifungal activity was determined by wells diffusion method, and determination of the total flavonoid was done by UV-Vis Spectrophotometer. Maceration of 700 grams of the trembesi leaves produced 68.95 grams of crude ethanol extract. The partition process using n-hexane, chloroform, and n-butanol produced 34.59, 8.92, and 4.23 grams of extracts respectively. The results of antifungal test on C. albicans showed that 30% of n-butanol extract had the highest activity compared to the extract of n-hexane and chloroform. The Minimum Inhibitory Concentration (MIC) value of n-butanol extract was 10% with an inhibition diameter of 7 mm.
AKTIVITAS ANTIBAKTERI EKSTRAK KULIT PISANG (Musa sp.) TERHADAP Escherichiacoli DAN Staphylococcus aureus SERTA IDENTIFIKASI GOLONGAN SENYAWA AKTIFNYA Ida Ayu Raka Astiti Asih; Wiwik Susanah Rita; I Gusti Bagus Teguh Ananta; Ni Kadek Dyan Mustika Sri Wahyuni
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 6 No 1 (2018): Volume 6, Nomor 1, 2018
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Kulit pisang (Musa sp.) dapat digunakan sebagai bahan penghambat bakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri kulit pisang (Musa sp.) terhadap Escherichia coli dan Staphylococcus aureus serta identifikasi senyawa aktifnya.. Ekstraksi dilakukan dengan teknik maserasi dengan etanol, selanjutnya ekstrak etanol dipartisi berturut-turut dengan n-heksana, etilasetat, dan n-butanol. Ekstrak n-heksana, etil asetat, dan n-butanol, yang diperoleh diuji fitokimia dan aktivitas penghambatan terhadap bakteri Escherechia coli dan Staphylococcus aureus. Uji penghambatan bakteri dilakukan dengan metode sumur difusi pada media Nutrient Agar (NA).Ekstraksi 500 g kulit pisang (Emas, Kepok, Susu dan Kayu) masing-masing menghasilkan 15, 20, 10, 12 gram ekstrak pekat yang berwarna kuning. Hasil uji fitokimia menunjukkan bahwa ekstrak kulit pisang mengandung senyawa alkaloid, terpenoid, fenol dan flavonoid. Hasilujiaktivitas terhadap bakteri Escherechia coli dan Staphylococcus aureus menunjukkan bahwa ekstrak kulit pisang Mas dan Kepok adalah ekstrak yang aktif dengan MIC masing masing 1% dan 0,5%, untuk kulit pisang mas dan 0,5% serta 0,1%, untuk kulit pisang kepok. Kata Kunci: Antibakteri, Musa sp., Escherichia coli, Staphylococcus aureus, Minimum Inhibitory Concentration (MIC) ABSTRACT: The banana peel (Musa sp.) can be used as antibacteria. This study aims to determine the antibacterial activity of banana peel (Musa sp.) against Escherichia coli and Staphylococcus aureus and to identify their active compounds. Extraction was done by maceration technique with ethanol, then ethanol extract was partitioned successively with n-hexane, ethylacetate, and n –butanol to get n-hexane, ethyl acetate, and n-butanol extract. The extract was tested phytochemical and activity of against Escherichia coli and Staphylococcus aureus. The antibacterial activity test was performed by diffusion well method on Nutrient Agar (NA) medium. Extraction of 500 g banana peel (Mas, Kepok, Susu and Kayu) each produced 15, 20, 10, 12 grams of yellow-colored concentrated extract. The phytochemical test results showed that banana peel extract contains alkaloids, terpenoids, phenols and flavonoids. The antibacterial activity test of the banana peels against Escherechia coli and Staphylococcus aureus showed that banana extract of Mas and Kepok was the active extract with MIC 1% and 0.5%, for mas and 0.5% and 0,1%, for kepok peel.
POTENSI EKSTRAK LIMBAH KULIT PISANG LOKAL (Musa sp) SEBAGAI ANTIBAKTERI TERHADAP Escherichia coli dan Staphylococcus Aureus I Gusti Bagus Teguh Ananta; Wiwik Susanah Rita; I Made Oka Adi Parwata
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 6 No 1 (2018): Volume 6, Nomor 1, 2018
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Penelitian ini dilakukan unuk menentukan potensi ekstrak limbah kulit pisang lokal sebagai antibakteri terhadap Escherichia coli dan Staphylococcus aureus. Penelitian yang dilakukan meliputi penentuan ekstrak limbah kulit pisang lokal (pisang mas, susu dan kayu) yang terbaik dalam menghambat pertumbuhan bakteri, penentuan pelarut terbaik, dan konsentrasi hambat minimum. Hasil penelitian menunjukkan bahwa ekstrak metanol merupakan ekstrak yang paling aktif dalam menghambat pertumbuhan bakteri dengan daya hambat sebesar 19,17 mm untuk bakteri Escherichia coli dan 18,67 mm untuk bakteri Staphylococcus aureus pada konsentrasi 10 %. Ekstrak n-butanol limbah kulit pisang mas memberikan daya hambat bakteri yang lebih baik dibandingkan ekstrak air, n-heksana, dan etil asetat yaitu sebesar 17,15 mm untuk bakteri Escherichia coli dan 16,37 mm untuk bakteri Staphylococcus aureus. Konsentrasi hambat minimum ekstrak n-butanol menunjukkan hasil hambatan pertumbuhan bakteri Escherichia coli adalah sebesar 0,5 % dengan daya hambat sebesar 7,65 mm dan 0,5% untuk bakteri Staphylococcus aureus dengan daya hambat sebesar 5,55 mm. Uji fitokimia menunjukkan bahwa senyawa yang berperan dalam aktivitasnya sebagai antibakteri yaitu alkaloid, terpenoid, flavonoid, fenol dan saponin. Kadar total fenol yang terdapat dalam ekstrak n-butanol limbah kulit pisang mas adalah sebesar 250,17 mg GAE/ 100 g dan kadar total flavonoid sebesar 129,07 mg QE/100g Kata Kunci : Antibakteri, Escherichia coli, Staphylococcus, Musa Paradisiaca, ABSTRACT: This research has been conducted to determine the potency of banana local peel waste (Musa sp) as antibacteria towards Escherichia coli and Staphylococcus aureus. The research included determining of the best extract of local banana peel waste (banana mas, susu, and kayu) to inhibit bacterial growth, the best solvent, and minimum inhibitory concentration. The results showed that the methanol extract has the best one to inhibit the growth of both bacteria E. coli and S. aureus with the inhibition zone of 19.17 mm and 18.67 mm respectively at concentration 10%. n-butanol extract of banana mas (Musa accuminata, AA) peel waste was the best one to inhibit both bacteria compared to water, n-hexane, and ethyl acetate extract with the inhibition zone of 17.15 mm for Escherichia coli and 16.37 mm for Staphylococcus aureus. The minimum inhibitory concentration of n-butanol extract towards both E. coli and S. aureus was 0.5% with the inhibitory zone of 7.65 mm and 5.55 mm respectively. Phytochemical screening result indicated the presence of alkaloid, terpenoid, phenols, flavonoids, and saponins which may influence the antibacterial activities. Total phenols content of the extract was 250.17 mg GAE/ 100 g while total flavonoids content was 129.07 mg QE/ 100 g.
PEMBUATAN BIOETANOL DARI CAMPURAN LIMBAH NASI DAN KULIT PISANG Ni Made Sukma Sanjiwani; Wiwik Susanah Rita; I Made Dira Swantara
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 6 No 2 (2018): Volume 6, Nomor 2, 2018
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Kulit pisang dan nasi merupakan limbah dari upacara keagamaan di Bali yang terbuang. Pembuatan bioetanol dilakukan untuk memanfaatkan limbah tersebut. Pada penelitian ini dilakukan pembuatan bioetanol campuran nasi dan kulit pisang. Campuran nasi dan kulit pisang yang digunakan dengan perbandingan 10:0; 7:3; 5:5; 3:7; dan 0:10. Tujuan dari penelitian ini adalah untuk menentukan kadar bioetanol yang diperoleh pada waktu fermentasi optimum. Penelitian ini dibagi menjadi beberapa tahap, yaitu tahapan persiapan sampel, hidrolisis nasi dan kulit pisang secara fisik dengan cara perebusan dan kimia dengan penambahan H2SO4, penentuan kadar gula pereduksi (Metode Nelson Semogyi), fermentasi, destilasi, dan penentuan kadar bioetanol menggunakan kromatografi gas. Kadar gula pereduksi tertinggi pada hidrolisis secara fisik (9,35%) dihasilkan dari campuran nasi dan kulit pisang (7:3) dengan waktu perebusan 50 menit, sedangkan kadar gula pereduksi tertinggi pada hidrolisis secara kimia (5,75%) dihasilkan dari campuran nasi dan kulit pisang (3:7) dengan konsentrasi H2SO4 1%. Waktu optimum yang diperlukan pada proses fermentasi campuran nasi dan kulit pisang pada hidrolisat secara fisik dan kimia menggunakan ragi tape berturut–turut 7 dan 6 hari, dengan kadar etanol masing–masing sebesar 4,17 dan 1,65%. Kata kunci: bioetanol, destilasi, fermentasi, gula pereduksi, hidrolisis, limbah kulit pisang dan nasi ABSTRACT: Banana peels and cooking rice are mostly becoming waste after Balinese religion ceremonies. Bioethanol production is carried out to utilize the waste. In this research, bioethanol mixture of cooking rice and banana peels is made. A mixture of cooking rice and banana peels used was with a ratio of 10:0; 7:3; 5:5; 3:7; and 0:10. The purpose of this research was to determine the content of bioethanol obtained at the optimum fermentation time. This research was divided into several stages, namely sample preparation stage, hydrolysis of cooking rice and banana peels physically by boiling and chemically with the addition of H2SO4, determination of reducing sugar content (Nelson Semogyi Method), fermentation, distillation, and determination of bioethanol content using Gas Chromatography. The highest reducing sugar content in physical hydrolysis (9,35%) was produced from a mixture of cooking rice and banana peels (7:3) with a boiling time of 50 minutes, while the highest reducing sugar content in chemical hydrolysis (5,75%) was produced from the mixture cooking rice and banana peels (3:7) with concentration H2SO4 1%. The optimum time required for the fermentation process on a hydrolyzate physically and chemically using yeast respectively 7 and 6 days, with ethanol content were respectively 4,17 and 1,65%.
POTENSI MINYAK ATSIRI RIMPANG JERINGAU (Acorus calamus Linn) SEBAGAI PENGHAMBAT PERTUMBUHAN Fusarium solani, JAMUR PATOGEN PENYEBAB BUSUK BATANG PADA BUAH NAGA Wiwik Susanah Rita; Ida Ayu Raka Astiti Asih; Ni Made Yuliari
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 4 No 2 (2016)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Isolasi dan uji aktivitas minyak atsiri rimpang jeringau (Acorus calamus Linn) sebagai penghambat pertumbuhan jamur patogen Fusarium solani telah dilakukan. Penelitian ini bertujuan untuk mengetahui aktivitas antijamur terhadap Fusarium solani dan kandungan utama dari minyak atsiri rimpang jeringau. Ekstraksi minyak atsiri dilakukan dengan metode destilasi uap, sedangkan uji aktivitas antijamur dilakukan dengan metode sumur difusi, dan identifikasi dilakukan dengan gas kromatografi – spektrometri massa (GC-MS). Ekstraksi 10 kg rimpang jeringau menghasilkan 13,39 gram minyak dengan hasil rendemen sebesar 0,1339%. Minyak atsiri memiliki warna kuning dan bau yang sangat tajam. Hasil uji aktivitas antijamur Fusarium solani terhadap minyak atsiri konsentrasi 10% menunjukkan aktivitas kuat dengan daya hambat sebesar 10,00 mm. Nilai Minimum Inhibitory Consentration (MIC) sebesar 2,0 % (v/v) dengan diameter hambat sebesar 5,50 mm. Hasil uji daya hambat pertumbuhan koloni, spora, dan biomassa jamur meningkat dengan kenaikan konsentrasi minyak atsiri. Analisis dengan gas kromatografi – spektrometri massa (GC-MS) menunjukkan bahwa komponen terbesar minyak atsiri jeringau adalah senyawa asaron.     ABSTRACT: Isolation and Activity test of Jeringau rhizome’s essential oil (Acorus Calamus Linn) to inhibit the growth of fungal pathogen, Fusarium solani has been performed. The aim of this research are to determine the antifungal activity and essential oil’s components of Jeringau rhizome. The extraction process was performed by steam distillation method, antifungal activity was analysed by well diffusion method, and the Gas Chromatography-Mass Spectrometry (GC-MS) was used to determine the essential oil components. The yield of essential oil was 0.1339% and has yellow colour with pungent smell. At concentration of 10.0%, the essential oil extract gave the strong activity to inhibit the Fusarium solani, with 10 mm in diameter. Minimum Inhibitory Concentration (MIC) was 2.0% that gave inhibition’s zone of 5.50 mm. Inhibition of colony, spore, and biomass of fungi increase with concentration. Analysis using GC-MS indicated the main essential compound is asarone.
AKTIVITAS ANTIOKSIDAN FRAKSI n-BUTANOL EKSTRAK KULIT TERONG BELANDA (Solanum betaceum Cav.) SECARA IN VITRO DAN IDENTIFIKASI SENYAWA GOLONGAN FLAVONOIDNYA Ni Putu Widayanti; Ni Made Puspawati; I Nyoman Suarsana; I.A. Raka Astiti Asih; Wiwik Susanah Rita
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 4 No 1 (2016)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan secara in vitro dan mengidentifikasi golongan senyawa flavonoid yang terkandung dalam fraksi n-butanol ekstrak kulit terong belanda. Uji aktivitas antioksidan dilakukan dengan metode DPPH. Pemisahan dan pemurnian fraksi aktif n-butanol dilakukan dengan kromatografi kolom menggunakan silika gel yang dielusi dengan n-butanol-etil asetat-asam asetat 10% (2:7:1) dan diidentifikasi dengan spektrofotometer UV-Vis dan FTIR. Fraksi n-butanol memiliki aktivitas antioksidan yang kuat dengan nilai IC50 sebesar 69,89 mg/L. Empat senyawa flavonoid (isolat A, C, H, dan J) berhasil diisolasi dan diidentifikasi sebagai golongan flavon, flavonol dan isoflavon. Senyawa tersebut dipercaya memiliki aktivitas antioksidan.     ABSTRACT: The present study was conducted to determine in vitro antioxidant activity and ctivity and to identify flavonoid class of compounds present in n-butanol active fraction of Terong Belanda (Solanum betaceum cav.) peel extract. Antioxidant activity test was performed by DPPH (1,1-diphenyl-2-pycrylhidrazyl) method. Separation and purification of n-butanol active fraction were done using silica gel column chromatography eluted with n-butanol-ethyl acetate-acetic acid 10% (2:7:1) and identified by UV-Vis and FTIR spectrophotometer. n-Butanol fraction had strong antioxidant activity with IC50 value by 69.89 mg/L. Four flavonoids (A, C, H, and J isolates) were isolated and identified as flavone, flavonol and isoflavone. This compounds are believed to be responsible for their antioxidant activity.    
FORMULASI SEDIAAN SABUN PADAT MINYAK ATSIRI SERAI DAPUR (Cymbopogon citratus DC.) SEBAGAI ANTIBAKTERI TERHADAP Escherichia coli DAN Staphylococcus aureus Wiwik Susanah Rita; Ni Putu Eka Vinapriliani; I Wayan Gede Gunawan
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 6 No 2 (2018): Volume 6, Nomor 2, 2018
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Minyak atsiri serai dapur (Cymbopogon citratus DC.) dapat digunakan sebagai alternatif pengganti antibakteri sintetik dalam pembuatan sabun mandi padat transparan. Penelitian ini bertujuan untuk mengetahui potensi serai dapur sebagai antibakteri terhadap Escherichia coli dan Staphylococcus aureus, formulasi terbaik minyak atsiri serai dapur sebagai sabun antibakteri, dan baku mutu SNI dari sabun mandi antibakteri yang dihasilkan. Penelitian ini terdiri dari 5 formula dan 3 kali ulangan. Formula pada penelitian ini adalah penambahan minyak atsiri serai dapur sebesar 0, 1, 2, 3, dan 5 g. Uji aktivitas antibakteri terhadap E. coli dan S. aureus dilakukan dengan metode sumur difusi, sedangkan uji kualitas sabun yang ditentukan adalah kadar air, jumlah asam lemak tersabunkan, asam lemak bebas/alkali bebas, lemak tak tersabunkan, dan minyak mineral. Hasil penelitian menunjukkan bahwa penambahan minyak atsiri serai dapur pada sabun transparan berpengaruh nyata terhadap aktivitas antibakteri terhadap E.coli, tetapi tidak berpengaruh terhadap S. aureus. Perlakuan terbaik pada penelitian ini adalah perlakuan penambahan minyak atsiri serai dapur 1 g (formula 2). Minyak atsiri dapat menghambat pertumbuhan E. coli dan S. aureus dengan daya hambat kuat. Diameter hambat terhadap E. coli sebesar 12,25; 12,25; dan 16,75 mm pada konsentrasi 25; 50; dan 100%, sedangkan daya hambat terhadap S. aureus sebesar 10,25; 10,50; dan 11,00 mm pada konsentrasi yang sama. Aktivitas antibakteri sabun transparan terhadap bakteri E. coli dan S. aureus juga tergolong kuat dengan diameter hambat keduanya antara 17-22 mm. Hasil uji kualitas sabun padat transparan sesuai dengan standar SNI kecuali fraksi tak tersabunkan. Kata kunci : antibakteri, Cymbopogon citratus DC., Escherichia coli, sabun, Staphylococcus aureus. ABSTRACT: Essential oil of lemongrass (Cymbopogon citratus DC.) was able to be used as an alternative to synthetic antibacterial in producing of transparent solid soap. The aim of this research is to study of lemongrass as antibacterial against Escherichia coli and Staphylococcus aureus, best formulation of lemongrass essential oil as antibacterial soap, and SNI quality standard of antibacterial soap. The study consisted of 5 formulas and 3 replications. The formula in this study was the addition of lemongrass essential oil of 0, 1, 2, 3, and 5 g. The antibacterial activity test against E. coli and S. aureus was done by diffusion well method, while the soap quality test determined was moisture content, the amount of saponified fatty acid, free fatty acids / alkali, unsaponified lipid, and mineral oil. The results shows that the addition of essential oil of lemongrass on transparent soap had a significant effect on antibacterial activity against E. coli, but did not affect to S. aureus. The best treatment in this research was the addition of essential oil of lemongrass 1 g (formula 2). Essential oils can inhibit the growth of E. coli and S. aureus with strong inhibition. The inhibitory zone towards E. coli was 12.25; 12.25; and 16.75 mm at concentration 25; 50; and 100% respectively, while that towards S. aureus was 10.25; 10.50; and 11.00 mm at the same concentration. The antibacterial activity of transparent soap against E. coli and S. aureus bacteria was also quite strong with both inhibitory diameter of 17-22 mm. The result of transparent solid soap quality test was in accordance with SNI standard except unsaponified fraction.
ANALISIS FENOL DALAM URIN PEKERJA SALAH SATU STASIUN PENGISIAN BAHAN BAKAR UMUM DI KOTA DENPASAR Abdul Rahim; Ni Made Suaniti; Wiwik Susanah Rita
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Phenol is a compound produced by the metabolism of benzene contained in the fuel. The purpose of this study was to determine the content of phenol in the urine of workers at a Public Gas Station in Denpasar. The method used in this analysis was gas chromatography – FID (GC-FID). Urine was taken 3 times with an interval of 7 days from 3 workers. Control was taken from a non-gas station worker. Standard solutions used were mixtures of benzene and phenol with concentration of 1.25, 2.50, 5, and 10 ppm. Phenol calibration curve equation was y = 2.94x ??+ 0.23 with a correlation coefficient (r) = 0.9997. The concentration of phenol of samples varied ranging from 0.51 ppm to 1.38 ppm, while the phenol content in gasoline alone was 0.66 ppm. Phenol levels in the urines were still under normal value which is 25 ppm, while the level of benzene exposure was still under the standard concentration of 10 ppm.
Co-Authors Abdul Rahim Adi Djona Silaen Ali Sabikin Frida R. Koreh Gede Widihati, Ida Ayu HERNINDYA, ANISA I Gusti Agung Gede Bawa I Gusti Ayu Kunti Sri Panca Dewi I Gusti Bagus Teguh Ananta I Kadek Pater Suteja I M, Sukadana I Made Adi Suardhyana I Made Dira Swantara I Made Oka Adi Parwata I Made Siaka I Nyoman Suarsana I Nyoman Suartha I W BUDIARSA SUYASA I Wayan Gede Gunawan I Wayan Suirta I. A. R. Astiti Asih Irdhawati Irdhawati Irvansyah Habibi Resaputra Iryanti Eka Suprihatin James Sibarani Kenanda, I Putu Bayu Mbatu, Rosanti Suryani Tince Ni Kadek Dyan Mustika Sri Wahyuni Ni Ketut Sinarsih Ni Ketut Sinarsih Ni Komang Ariati Ni Luh Putu Lilis Wirastiningsih Ni Luh Rustini Ni Luh Yuli Damayanti Ni Made Dwita Prasetyawati Ni Made Puspawati Ni Made Puspawati Ni Made Suaniti Ni Made Sukma Sanjiwani Ni Made Yuliari Ni Putu Eka Vinapriliani Ni Putu Widayanti Putu Prisanti Putri Utami Putu Puspita Sari Putu Sariningsih Raditya Yoga Rahayu, Riana Suastari Retno Kawuri Rr Anisa Hernindy Sinarsih, Ni Ketut Suharta, I Gede Yeyen Valent, Febryna Aurora WAHYU DWIDJANI S Wirajana, Nengah Yenni Ciawi Yohanes Armawan Sandi