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All Journal HAYATI Journal of Biosciences CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Kimia (Journal of Chemistry) Buletin Udayana Mengabdi Jurnal Veteriner Ecotrophic, Journal of Environmental Science Indonesian Journal of Cancer Jurnal Media Sains Journal of Health Sciences and Medicine INTERNATIONAL JOURNAL OF APPLIED CHEMISTRY RESEARCH Indonesian Journal of Cancer

WIWIK SUSANAH RITA

Chemistry Department, Faculty Of Mathematic And Natural Sciences Universitas Udayana Badung, Bali

Author-ID : 361870

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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PENGARUH KONSENTRASI AMMONIUM SULFAT ((NH4)2SO4) SEBAGAI SUMBER NITROGEN TERHADAP PRODUKSI BIOETANOL BERBAHAN BAKU Glacilaria sp. Ni Putu Widayanti; Wiwik Susanah Rita; Yenni Ciawi
Jurnal Kimia (Journal of Chemistry) Vol. 7, No. 1 Januari 2013
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

The aims of this research are to determine the concentration of reducing sugar and starch in Glacilaria sp. and to investigate the influence of the concentration of ammonium sulfate as nitrogen source on the production of bioethanol using Glacilaria sp. as the carbon source. The concentrations of ammonium sulfate employed were 0,25%; 0,50%; 0,75%; and 1% (w/v). The methods employed in this research were the determination of water content, glucose and starch concentration using Nelson-Somogyi method, determinating the optimum duration of fermentation, pH measurement, and the determination of ethanol concentration. The results showed that the concentrations of reducing sugar and starch in Glacilaria sp. are 17,14% and 15,42%. The concentration of (NH4)2SO4, which produced the optimum concentration of ethanol (1,04% (v/v) on seventh day), was 1% (w/v). The fermentation media containing (NH4)2SO4 of 1%; 0,75%; 0,5%; and 0,25% (w/v) produced optimum concentrations of ethanol, which were 0,96% (v/v) on fifth day; 0,78% (v/v) on seventh day; 0,74% (v/v) on seventh days; and 0,73% (v/v) on seventh day, respectively. The optimum concentration of (NH4)2SO4 was 1% (w/v), which produced maximum concentration of bioethanol from Glacilaria sp., of 0,96% (v/v).

EFEK EKSTRAK ETANOL BIJI JAGUNG (ZEA MAYS) TERHADAP PROFIL LIPID TIKUS WISTAR DENGAN DIET TINGGI LEMAK Ni Luh Rustini; Komang Ariati; Wiwik Susanah Rita
Jurnal Kimia (Journal of Chemistry) Vol. 11. No.2 Juli 2017
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Pada penelitian ini dipelajari efek ekstrak etanol biji jagung (Zea mays) terhadap profil lipid tikus wistar dengan diet tinggi lemak. Penelitian ini bertujuan untuk membuktikan efek antihiperkolesterolemia dari ekstrak etanol biji jagung dengan variasi dosis 50 mg/Kg BB; 100 mg/Kg BB dan 150 mg/Kg BB. Subjek penelitian ini ialah 24 ekor tikus Wistar yang dibagi menjadi 6 kelompok secara random dengan design penelitian posttest only control group design. Data hasil penelitian di analisis dengan uji tukey anova. Ekstrak etanol biji jagung dapat menurunkan kadar kolesterol total tikus wistar dengan diet tinggi lemak dengan dosis 50 mg/Kg BB, 100 mg/Kg BB dan 150 mg/Kg BB sebesar 5,44%; 12,89% dan 19,77%. Kadar kolesterol LDL dapat diturunkan dengan dosis 50 mg/Kg BB, 100 mg/Kg BB dan 150 mg/Kg BB sebesar 2,55%; 8,74% dan 15,66%. Kadar kolesterol HDL dapat ditingkatkan dengan dosis 50 mg/Kg BB, 100 mg/Kg BB dan 150 mg/Kg BB sebesar 1,5%; 2,08% dan 3,24%. Hasil uji fitokimia menunjukkan bahwa ekstrak etanol biji jagung positif mengandung senyawa golongan steroid dan fenol. Senyawa tersebut memiliki aktivitas antioksidan dan dapat meningkatkan antioksidan endogen di dalam tubuh, sehingga dapat menurunkan kolesterol darah.

PEMANFAATAN KALENG ALUMINIUM BEKAS DALAM MENANGANI PENCEMARAN AIR WIWIK SUSANAH RITA; I.A. RAKA ASTITI ASIH; WAHYU DWIDJANI S; I W. SUIRTA; I G.A. KUNTI SRI PANCA DEWI
Buletin Udayana Mengabdi Vol 7 No 2 (2008): Volume 7 No.2 – September 2008
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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ABSTRACT Community service by giving a lecture and practical work with a title: “Utilizing of Aluminum Cans used for Water Purification (Pemanfaatan Kaleng Aluminium Bekas dalam Upaya Penjernihan Air)” was be held on Wednesday, 22nd of August 2007. The participants of this activity were about 45 of the Third degree Students of SMAN Abiansemal Badung. The aim of the activity was to give information that aluminum cans can be recycled into alum (tawas), where it can be used for water purification. The result of this activity indicated that the knowledge of the students have broaden. This was showed by Their enthusiastic to give some questions and ideas. They paid attention about the environmental cleanliness, so this activity can help them to solve many environmental problems and motivate them to run a private enterprise.

Ekstrak Etanol dan Fraksi Heksan Buah Pare (Momordica charantia) Sebagai Penurun Kadar Glukosa Darah Tikus Diabetes (ETHANOL EXTRACT AND HEXANE FRACTION OF MOMORDICA CHARANTIA DECREASE BLOOD GLUCOSE LEVEL OF DIABETIC RAT) I Nyoman Suartha; I Made Dira Swantara; Wiwik Susanah Rita
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The research on the potency of Momordica charantia as lowering blood glucose has been carried out.The fruit was extracted by 70 % ethanol at room temperature. The extract was then partition with NHexane.The filtrate of partition was purified with thin layer chromatography. Three months old of whitemale rats (Rattus novergicus) with approximately 200-250 grams in weight were used as Bio-indicators.This study used a randomized block design (CRD) consisting of eight treatment groups (each treatmentconsisted of five rats). Rats were injected with streptozotocin to get hyperglycemic condition. The M.charantia fruit fraction (fraction 1-5) with dose 100 mg/kg bw was treated to each group when the ratt wason hyperglycemic condition. Rat blood glucose levels were observed on days 0, 4, 11, and 18 respectively .The results showed that blood glucose levels of M. charantia fraction 1and 5 with dose of 100 mg/kg bwhave the same effect with a negative control from day 4th, fraction 2 on day 18 whereas The others fractionare 3, and 4 were effect on days 18th. Based on the result it can be concluded that the M. charantia fraction1 with dose of 100 mg/kg bw effectively in decreasing the blood glucose levels of diabetic rat.

In Vitro Evaluation Of Antioxidant Activity Of Flavonoid Compounds From Terong Belanda Ida Ayu Raka Astiti Asih; Ni Made Puspawati; Wiwik Susanah Rita
Journal of Health Sciences and Medicine Vol 1 No 2 (2017): JHSM (September 2017)
Publisher : Institute for Research and Community Services Udayana University

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Terong belanda (Solanum betaceum, cav), is a fruit that has nutrients and vitamins which are essential for health of human body and it is believed to have a potent antioxidant activity. This research aimed to evaluate in vitro antioxidant activity of flavonoid compounds isolated from Terong belanda. In vitro antioxidant activity was evaluated using DPPH method. Identification of flavonoids was done using Infrared and UV–Vis Spectrophotometers. Extraction of ten (10) kg terong belanda fruits with ethanol gave of 126.17 g peels extract, 178.44 g flesh, and 253.11 g seed extracts respectively. Phytochemical test showed both peels, flesh, and seed extracts of Terong belanda contained flavonoids. Antioxidant activity test showed peels, flesh, and seeds extracts exhibited antioxidant activity with IC50 of 68,14 ppm, 621.45 ppm, and 1162.608 ppm respectively. Separation and purification of these three active extracts using column chromatography gave each one isolate which positive flavonoid on phytochemical test. Infrared spectra of each isolate from peels, flesh, and seed were similar and each revelaed to contain the same functional groups (OH alcohol, CH aromatic, C=O, C=C, and CH aliphatic). Analysis of UV-Vis spectra and its spectra with shifting reagents of the peels isolates gave absorption at the wavelength range of 310-330 nm (Bans I ) and 245-275 nm (bands II) due to isoflavones group with a hydroxy group at C6, C7 or C7, C8 and C3', C 4 ' while flesh and seed gave absorption at 300-330 nm (bands I) and 275-295 nm (bands II) that indicated the class of flavanones with hydroxyl groups at C-2', C-5', C-6' and Oglycoside at C7 for flessh and hydroxyl group at C6, C7 or C7, C8 for the seed.

The Extraction and Phytochemical Test of Toxic Extract of Xestospongia testudinaria Sponge I Made Diraswantara; Wiwik Susanah Rita
Journal of Health Sciences and Medicine Vol 2 No 1 (2018): JHSM (February 2018)
Publisher : Institute for Research and Community Services Udayana University

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This research aims toextraction and phytochemical test of the toxic extract of the Xestospongia testudinaria sponge collected from Sanur beach, Bali, Indonesia. Extraction of the sponges was carried out by methanol at room temperature. Toxicity screening test was done based on Bhrine Shrimp Lethality Test (BSLT). The compounds of the toxic extract were performed by phytochemical test. Based on the results, it was found that the methanol extract of X. testudinaria sponges has toxic activity with LC50 of 31.62 ppm. The toxic extract contained alkaloid, steroid, polyphenol, and saponin compounds.

THE ESSENTIAL OIL CONTENTS OF JERINGAU (Acorus calamus L.) RHIZOMES AND THEIR ANTIFUNGAL ACTIVITY AGAINST Candida albicans Wiwik Susanah Rita; Retno Kawuri; I Made Dira Swantara
Journal of Health Sciences and Medicine Vol 1 No 1 (2017): JHSM (Febuary 2017)
Publisher : Institute for Research and Community Services Udayana University

Acorus calamus L. rhizome was trusted having antibacterial activity. This study aimed to identify the compounds in the Acorus Calamus L. rhizomes essential oils and to recognize the antifungal activity of the oils against Candida albicans. The extraction of essential oils from rhizome was carried out by steam distillation technique. Identification of compounds in the oils was conducted by Gas Chromatography- Mass Spectroscopy (GCMS), while the antifungal test against Candida albicans was done by well diffusion method. Extraction of 10 kg of rhizomes produced 16.53 mL essential oil with a yield of 0.1653% (? = 1.066), the oil was brownish yellow and very flavorful. GC-MS analysis showed that the essential oil contained 11 compounds, they are (E)-3,7 dimethyl-1,3,6-Octatriene (trans-?-Ocimene) (3,73%), linalool (1,07%), ?-elemene (1,15%), trans methyl isoeugenol (7,68%), shyobunon (15,74%), bicyclogermakren (0,93%), dehidroxy-isocalamendiol (2,61%), ?-calacorene (3,34%), euasarone (26,84), cis-asarone (18,62%); dan trans- asarone (18,29%). Antifungal activity test showed that the growth and biomass inhibition of C. albicans increased with the increase of the oil concentration. Minimum Inhibitory Concentration (MIC) of essential oil toward C. albicans was 1% with the inhibition of 7.83 mm.

ISOLATION AND PHYTOCHEMICAL TEST OF ANTICANCER ISOLATE OF SPONGE Hyrtios erecta I Made Dira Swantara; Wiwik Susanah Rita; Rr Anisa Hernindy
Journal of Health Sciences and Medicine Vol 1 No 1 (2017): JHSM (Febuary 2017)
Publisher : Institute for Research and Community Services Udayana University

AbstractThis research aims to isolate and phytochemically test of the toxic isolate from ethanol extract of the sponge Hyrtios erecta taken from the waters of Pari Island beach, Thousand Islands (Jakarta). Extraction of the sponges was carried out by 70% ethanol at room temperature. Partition and purification of the compounds were done by column chromatography with the stationary phase of silica gel and the mobile phase of n-hexane-chloroform (2:8). Toxicity screening test was done based on Bhrine Shrimp Lethality Test (BSLT). The compounds of the active isolate were performed by phytochemical test. Based on the results, it was found that the toxic isolate of Hyrtios erecta sponges has anticancer activity with IC50 of 30,497 ppm. The anticancer isolate contained alkaloid, steroid, and polyphenol compounds

AKTIVITAS ANTIBAKTERI FRAKSI EKSTRAK ETANOL DAUN TREMBESI (SAMANEA SAMAN (JACQ.) MERR) TERHADAP STAPHYLOCOCCUS AUREUS Ni Ketut Sinarsih; Wiwik Susanah Rita; Ni Made Puspawati
International Journal of Applied Chemistry Research Vol. 3 No. 1 (2021)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijacr.v3i1.32860

Penelitian ini dilakukan untuk menentukan aktivitas antibakteri fraksi-fraksi dari hasil partisi ekstrak etanol daun trembesi dalam menghambat pertumbuhan bakteri S. aureus, yang meliputi ekstraksi daun trembesi dengan pelarut etanol, partisi menggunakan pelarut n-heksan, etil asetat, dan n-butanol, penentuan daya hambat fraksi hasil partisi, serta penentuan Konsentrasi Hambat Minimum (KHM) dari fraksi yang menunjukkan aktivitas penghambatan terbaik untuk menghambat pertumbuhan S. Aureus. Hasil penelitian menunjukkan bahwa dari 2000 gram sampel diperoleh ekstrak kental sebanyak 160 gram. Proses partisi menghasilkan empat fraksi yaitu fraksi n-heksan yang berwarna hijau tua, fraksi etil-asetat yang berwarna hijau kecoklatan, fraksi n-butanol yang berwarna coklat tua, dan fraksi air yang berwarna coklat pekat. Dari keempat fraksi, hanya fraksi n-butanol yang memberikan efek penghambatan terhadap S. aureus secara in vitro pada media Mueller Hinton, yaitu sebesar 19,3 mm pada konsentrasi 15%. Pada konsentrasi tersebut, aktivitas antibakteri fraksi n-butanol dapat dikategorikan kuat terhadap S. aureus. Pengujian KHM dilakukan pada tiga konsentrasi yaitu 0,5; 1; dan 1,5% dengan diameter hambat aktivitas antibakteri secara berturut -turut sebesar 0 mm; 7,3 mm; dan 9,2 mm.

Potensi Antikanker Isolat Toksik Tiga Spons Indonesia MADE DIRA SWANTARA; WIWIK SUSANAH RITA; JAMES SIBARANI
Indonesian Journal of Cancer Vol 7, No 4 (2013): Oct - Dec 2013
Publisher : National Cancer Center - Dharmais Cancer Hospital

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33371/ijoc.v7i4.311

Telah dilakukan uji antikanker isolat toksik 3 (tiga) jenis spons yang berasal dari perairan Indonesia. Ketiga spons tersebut adalah Callyspongia aerizusa, Haliclona fascigera, dan Lanthella basta. Isolasi metabolit dalam spons dilakukan dengan cara maserasi, kemudian dilanjutkan dengan tahap pemisahan dan pemurnian menggunakan cara partisi serta kromatografi kolom. Skrining toksisitas dilakukan dengan metode Bhrine Shrimp Lethality Test (BSLT). Uji antikanker secara in vitro isolat yang paling toksik menggunakan sel HeLa. Berdasarkan hasil penelitian ini diperoleh bahwa spons Callyspongia aerizusa bersifat sebagai antikanker dengan LC50 sebesar 5,50 ppm. Spons Haliclona fascigera tidak bersifat antikanker terhadap sl HeLa, karena harga LC50 sebesar 44,67 ppm. Sedangkan spons Lanthella basta bersifat antikanker dengan harga LC50 sebesar 18,62 ppm.Kata Kunci: aktivitas antikanker; Callyspongia aerizusa; Haliclona fascigera; Lanthella basta

Co-Authors Abdul Rahim Adi Djona Silaen Ali Sabikin Frida R. Koreh Gede Widihati, Ida Ayu HERNINDYA, ANISA I Gusti Agung Gede Bawa I Gusti Ayu Kunti Sri Panca Dewi I Gusti Bagus Teguh Ananta I Kadek Pater Suteja I M, Sukadana I Made Adi Suardhyana I Made Dira Swantara I Made Oka Adi Parwata I Made Siaka I Nyoman Suarsana I Nyoman Suartha I W BUDIARSA SUYASA I Wayan Gede Gunawan I Wayan Suirta I. A. R. Astiti Asih Irdhawati Irdhawati Irvansyah Habibi Resaputra Iryanti Eka Suprihatin James Sibarani Kenanda, I Putu Bayu Mbatu, Rosanti Suryani Tince Ni Kadek Dyan Mustika Sri Wahyuni Ni Ketut Sinarsih Ni Ketut Sinarsih Ni Komang Ariati Ni Luh Putu Lilis Wirastiningsih Ni Luh Rustini Ni Luh Yuli Damayanti Ni Made Dwita Prasetyawati Ni Made Puspawati Ni Made Puspawati Ni Made Suaniti Ni Made Sukma Sanjiwani Ni Made Yuliari Ni Putu Eka Vinapriliani Ni Putu Widayanti Putu Prisanti Putri Utami Putu Puspita Sari Putu Sariningsih Raditya Yoga Rahayu, Riana Suastari Retno Kawuri Rr Anisa Hernindy Sinarsih, Ni Ketut Suharta, I Gede Yeyen Valent, Febryna Aurora WAHYU DWIDJANI S Wirajana, Nengah Yenni Ciawi Yohanes Armawan Sandi

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