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All Journal HAYATI Journal of Biosciences CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Kimia (Journal of Chemistry) Buletin Udayana Mengabdi Jurnal Veteriner Ecotrophic, Journal of Environmental Science Indonesian Journal of Cancer Jurnal Media Sains Journal of Health Sciences and Medicine INTERNATIONAL JOURNAL OF APPLIED CHEMISTRY RESEARCH Indonesian Journal of Cancer

WIWIK SUSANAH RITA

Chemistry Department, Faculty Of Mathematic And Natural Sciences Universitas Udayana Badung, Bali

Author-ID : 361870

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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ISOLASI DAN IDENTIFIKASI SENYAWA YANG BERPOTENSI SEBAGAI ANTITUMOR PADA DAGING BUAH PARE (Momordica charantia L.) Wiwik Susanah Rita; I W. Suirta; Ali Sabikin
Jurnal Kimia (Journal of Chemistry) Vol. 2, No. 1 Januari 2008
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Isolation and identification of the compound which has a potency as antitumor from bitter melon have beencarried out. Extraction was conducted n-hexane, chloroform, and ethanol respectively using each extracts obtainedwere examined with brine shrimp lethality test. The most toxic extract was ethanol extract (LC50 223 ppm).Separation and purification of the compounds from the ethanol extract were conducted by column chromatogaraphyusing a gel silica 60 as the stationary phase and benzene : acetic acid ( 8:2) as the mobile phase. This yielded 3fractions. Then the fractions were examined with brine shrimp lethality test and the most toxic fraction was found tobe the fraction 1 (LC50 31,62 ppm), but the fraction that was analysed further was fraction 3 (LC50 100 ppm),because fraction 1 consists of using compounds that were difficult to separate. The purity fraction 3 was testedconducted thin layer chromatography and its activity as antitumor agent was tested using Agrobacterium tumefacienA-208. The test was in 6 weeks and that fraction 3 has a potency as an antitumor agent at 1000 ppm.The identification with gas chromatography – mass spectroscopy indicate that the antitumor isolate frombitter melon contains 3 mayor compoundsnamely dioxtyl hexadioate esther, palmitic acid, stearic acid.

ISOLASI, IDENTIFIKASI, DAN UJI AKTIVITAS ANTIBAKTERI SENYAWA GOLONGAN TRITERPENOID PADA RIMPANG TEMU PUTIH (Curcuma zedoaria (Berg.) Roscoe) Wiwik Susanah Rita
Jurnal Kimia (Journal of Chemistry) Vol. 4, No. 1 Januari 2010
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Isolation, identification, and antibacterial activity examination of triterpenoid compounds from rhizome ofCurcuma zedoaria (Berg.) Roscoe have been conducted. Maseration of 600 g dry powder of those rhizome using nhexaneand ethanol respectively yielded 9,79 g of n-hexane extract and 23,45 g of ethanol extract. The ethanolextract containing triterpenoids was dissolved into ethanol-water (7:3) mixture. The mixture was evaporated toremove the ethanol and then partitioned by chloroform. Hence, two extract were produced i.e., chloroform and waterextract. The n-hexane and chloroform extracts obtained contained triterpenoids based on fitochemical test ofLieberman-Burchard. Antibacterial test showed that only chloroform extract was active with inhibition zone of 2 mmfor Staphylococcus aureus.Compound separation of the chloroform extract using column chromatography produced 0,44 g brownisolate which contained triterpenoids (fraction F1). The isolate inhibited the growth of Staphylococcus aureus andEscherichia coli with weak inhibition zone at 500 ppm and 1000 ppm. Infrared spectra indicated that the isolate wasa carboxylic acids triterpenoids, with characteristic functional groups of –OH bonded, –CH, C=O of carboxylic acids,–C=C, –CH2, –CH3, and C–O alcohol. The ultraviolet-visible spectra showed maximum absorption at 242 nm and280 nm.

IDENTIFIKASI DAN UJI AKTIVITAS SENYAWA FLAVONOID DARI EKSTRAK DAUN TREMBESI (Samanea saman (Jacq.) Merr) SEBAGAI PENGENDALI JAMUR Fusarium sp. PADA TANAMAN BUAH NAGA Putu Sariningsih; Wiwik Susanah Rita; Ni Made Puspawati
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

This study aimed to examine antifungal activity of Trembesi (Samanea saman (Jacq.) Merr) leaves extract against Fusarium sp on dragon fruit and to identify types of flavonoid compounds present in the extracts. Isolation of the flavonoids was started by maceration followed by fractionation into n-hexane, chloroform, and ethyl acetate respectively. Separation was carried out by preparative layer chromatography while identification was done using Ultraviolet-Visible and Infrared spectrophotometer. Antifungal activity test showed that ethylacetate extract has mild activity in inhibiting the growth of Fusarium sp. (inhibition zone 6.75 mm). The antifungal activity testing of three isolates positive flavanoid (B4, B5, B6) showed at the concentration of 10 % they have not given activity yet. The infrared spectra of isolates (B4, B5, B6) were very similar, therefore they have the same functional groups (OH, C-OH, aromatic CH, aliphatic CH, C=O, C-O-C ether, and aromatic C=C). The UV-Vis spectra showed isolates B4 gave absorption at a wavelength of 336.00 nm (band I) and 268.40 nm (band II), isolates B5 at 269.20 nm (bands II), and 325.40 nm (band I), and isolates B6 at 475.40 nm (bandI) and 282.40 nm (band II). Further UV-Vis identification using shift reagents suggested that isolates B4 was tentatively identified as 3,7,8,4 ', 5' pentahydroxy flavonols, isolates B5 as 3,5,4 'trihydroxy flavones, and isolate B6 as 3,5,7,8,3', 4 'hexahidroxy anthocyanine.

HIDROLISIS RUMPUT LAUT (Glacilaria sp.) MENGGUNAKAN KATALIS ENZIM DAN ASAM UNTUK PEMBUATAN BIOETANOL Yohanes Armawan Sandi; Wiwik Susanah Rita; Yenni Ciawi
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 1 Januari 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

The aim of this research is to determine the effect of enzyme and acids concentration on the yield of glucose produced in the hydrolysis of Glacilaria sp. in the production of bioethanol. The concentrations of cellulase used were 200 units/mL, 400 units/mL, 600 units/mL, 800 units/mL and the concentration of sulphuric acid (H2SO4) and hydrochloric acid (HCl) used were 1%, 3%, 5%, 7% (w/v). The concentration of reduction sugar was determined using Anthrone and analyzed using UV-Vis spectrophotometry and the determination of ethanol concentration was carried out by using gas chromatography. The results showed that the contents of reducing sugar produced by sulphuric acid (H2SO4) hydrolysis were 26,19%; 36,69%; 41,40%; 45,0% (v/v), by hydrochloric acid (HCl) were 12,12%; 14,03%; 15,17%; 16,50% (v/v), and by cellulase enzyme were 46,15%; 46,73%; 47,68%; 48,25% (v/v). Optimum concentration of reducing sugar produced by hydrolysis using 800 units/mL cellulase was 48,25% (v/v). The optimum length of fermentation to produce bioethanol using Glacilaria sp. as raw material was 5 days. In the fermentation, inoculum with a concentrations of 5% and 10% (w/v) produced 0,85% and 1,51% (v/v) ethanol.

IDENTIFIKASI DAN UJI AKTIVITAS SENYAWA TANIN DARI EKSTRAK DAUN TREMBESI (Samanea saman (Jacq.) Merr) SEBAGAI ANTIBAKTERI Escherichia coli (E. coli) Putu Puspita Sari; Wiwik Susanah Rita; Ni Made Puspawati
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Isolation and identification of tannin compounds from trembesi leaves (Samanea saman (Jacq.) Merr) and its anti-bacterial activity test against Escherichia coli (E. coli) have been done in this research based on the utilization of trembesi leaves to treat diarrhea. Extraction was done by maceration and partition, separation by preparative TLC. The anti-bacterial activity was tested using wells that diffusion method, and the identification of the compounds was done with UV-vis spectrophotometer and FTIR. Maceration with ethanol produced 36.80 g crude ethanol extract. Phytochemical test showed that acetone and water fractions gave positive result for hydrolyzed tannin compounds but acetone fraction revealed more concentrated than water fraction. Anti-bacterial test result showed that the acetone fraction was active towards E.coli with medium activity. Separation of the eluent n-butanol: acetic acid: water (4:1:5) (BAA) gave six isolates but only two isolates (isolate 2 with Rf 0.61, and isolates 3 with Rf.0.65) gave positive results for tannin. These two isolates were relatively pure on TLC purity test and showed weaker anti-bacterial activity compared to the acetone fractions. Identification using UV-Vis spectrophotometer showed that isolates 2 and 3 gave two similar peaks with maximum absorbance at 346.50 nm and 347.00 nm respectively due to n?*and ??* electron transitions, which indicated the presence of C=O and C=C chromophores. Infrared spectra of isolates 2 and 3 revealed peaks that correspond to characteristic functional groups of tannin including –O-H, C-H aliphatic, C=O esther, C=C aromatic, C-O-H, and C-O-C ether.

IDENTIFIKASI DAN UJI AKTIVITAS SENYAWA FLAVONOID DARI EKSTRAK DAUN TREMBESI (Albizia saman (Jacq.) Merr) SEBAGAI ANTIBAKTERI Escherichia coli I Kadek Pater Suteja; Wiwik Susanah Rita; I Wayan Gede Gunawan
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 1 Januari 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Identification of flavonoid from the leaves of rain tree (Albizia saman (Jacq.) Merr) and its antibacterial activity test against Escherichia coli (E.coli) has been performed. This research aims to determine the type of flavonoid in rain tree leaves and its antibacterial activity against E. coli. Extraction was done by maceration and partition methods, separation was achieved by column chromatography, antibacterial activity was tested by disk diffussion method, and the identification was done by Ultraviolet-visible (UV-vis) and Infrared spectrophotometry. Extraction of 1 kg of rain tree powder with 5 L ethanol produced 73.38 g of concentrated ethanol extract. The partition process produced 26,34 g of n-hexane, 8,12 g of ethylacetate, 19,37 g n-butanol, and 12,56 g of water extracts. Phytochemical test of the extracts showed that the n-butanol extract contained flavonoids. Antibacterial activity of n-butanol extract towards E.coli showed a medium activity with a diameter inhibition of 6.3 mm. MIC value ??was 2% (w/v) with a diameter inhibition of 1 mm. Separation using column chromatography with the eluent of n-butanol: methanol: chloroform (5: 3: 2) obtained four isolates but only one isolate (isolate B with Rf 0.58) which contained flavonoids. Analysis with infrared spectroscopy showed that the isolate B contained functional groups of OH, C-OH, aliphatic CH, C = O ketones, C = C aromatic, COC ether, and aromatic CH. Analysis with UV-Vis spectrophotometer indicated that isolate B is isoflavon compound with a hydroxy groups at C-5 and C-7. The isolate was relatively pure by TLC. The isolate showed a low antibacterial activity.

ISOLASI DAN IDENTIFIKASI SENYAWA ANTIMAKAN DARI BATANG TUMBUHAN BROTOWALI (Tinospora tuberculata BEUMEE.) I M. Sukadana; Wiwik Susanah Rita; Frida R. Koreh
Jurnal Kimia (Journal of Chemistry) Vol. 1, No. 2 Juli 2007
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Isolation and identification of antifeedant compounds from brotowali stem (Tinospora tuberculataBEUMEE.) was done. Brotowali dry powder of 1 kg was extracted using methanol by maceration, The methanolextract then was fractionated repeatedly by n-hexane, so that methanol and n-hexane extracts were obtained. Both ofextracts were evaporated by rotary vacuum evaporator, so concentrated methanol and n-hexane extracts wereobtained and then their antifeedant activity was tested. More active extract was separated by thin layerchromatography (TLC) then continued by column chromatography utilizes silica gel 60 as a stationary phase and thebest mobile phases of TLC. Fractions obtained were tested of their antifeedant activity. More active fraction thenwas tested its purity and identified by phytochemical test and UV-vis and infrared spectrophotometer.As much as 97.07 g of concentrated methanol extract was resulted from 1 kg brotowali dry powder.Fractionation of the methanol extract using n-hexane resulted 22.92 g of concentrated methanol and 21.71 g ofconcentrated n-hexane. n-hexane extract points out more activity as antifeedant than methanol extract. The bestmobile phase of TLC was n-hexane: chloroform (1:1 ). The unite result from the column chromatography wasobtained 3 fractions where fraction c points out antifeedant active. The purity test of fraction c was obtained 1 spotso continued by phytochemical test and UV-vis and infrared spectrophotometer. Phytochemical test andspectrophotometry analysis from active isolate (fraction c) point out that the compound included triterpenoid groupthat absorb on wavelength 288.6 nm and 310.6 nm, and be considered having functional group of O-H bonded, CO,C-H, C=O, C=C, and C-H.

SKRINING AWAL ANTITUMOR MELALUI PENDEKATAN UJI TOKSISITAS KANDUNGAN SENYAWA DALAM EKSTRAK n-HEKSANA RIMPANG TEMU PUTIH (Curcuma zedoaria (Berg.) Roscoe) Wiwik Susanah Rita; I G. A. Gede Bawa; Ni Luh Putu Lilis Wirastiningsih
Jurnal Kimia (Journal of Chemistry) Vol. 6, No. 1 Januari 2012
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Isolation and identification of cytotoxic compounds from n-hexane extract of white turmeric rhizomes (Curcuma zedoaria (Berg.) Roscoe) has been performed. Extraction was done by maceration technique. Saponification reaction was applied to separate the fat with another lipid, separation and purification was then performed by chromatographic techniques. Toxicity tests performed by the method of Brine Shrimp Lethality Test (BSLT) using Artemia salina L. larvae, while the analysis of the most toxic isolates were performed by Gas Chromatography-Mass Spectroscopy (GC-MS).Extraction of 1700 g of dried white turmeric rhizome powder produced 39.68 g of concentrated n-hexane extracts. The results of toxicity tests with n-hexane extract of Artemia salina L. larvae was obtained LC50 values of 79.43 ppm. Saponification of n-hexane extract produced 9.18 g of n-hexane phase and 137.38 g water phase. Toxicity test indicated that n-hexane extract phase was the most toxic with the LC50 of 17.78 ppm. Then the active phase was separated and purified by gradient column chromatography and obtained 11 fractions. Fraction 11 was the the most toxic with the LC50 of 3.8 ppm. The column chromatography obtained two fractions (F11a and F11b), which F11b was the most active with LC50 of 3.5 ppm.The analysis of isolate by Gas Chromatography Mass Spectroscopy showed a mixture of compounds: tetradecane, hexadecane, 3-methylheptadecane, octadecane, 2-methyleicosane, n-docosane, and heneicosane.

TOKSISITAS SENYAWA FLAVONOID DARI EKSTRAK ETANOL DAUN DEWANDARU (Eugenia uniflora Linn.) SEBAGAI SKRINING AWAL ANTIKANKER I Made Dira Swantara; Wiwik Susanah Rita; I Made Adi Suardhyana
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 2 Juli 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Flavonoid merupakan senyawa yang dipercaya berpotensi sebagai antikanker. Salah satu tanaman yang mengandung flavonoid adalah tanaman Dewandaru (Eugenia uniflora Linn.). Penelitian ini bertujuan untuk melakukan identifikasi dan uji toksisitas senyawa flavonoid dari ekstrak etanol daun Dewandaru (Eugenia uniflora Linn.) yang berpotensi sebagai antikanker. Ekstraksi dilakukan dengan metode maserasi dan partisi menggunakan n-heksana, kloroform, dan etil asetat. Pemisahan fraksi kloroform dilakukan dengan kromatografi kolom silika gel menggunakan campuran pelarut kloroform : etil asetat (5:1) sebagai fase gerak. Uji toksisitas dilakukan dengan metode Brine Shrimp Lethality Test (BSLT), dan identifikasi dilakukan dengan spektrofotometer IR serta UV-Vis. Ekstraksi 1000 g serbuk daun Dewandaru dengan 10 L etanol 70% menghasilkan 273 g ekstrak pekat etanol dan proses partisi berturut – turut menghasilkan 30,14 g ekstrak pekat air, 5,58 g ekstrak pekat n-heksana, 20,57 g ekstrak pekat kloroform, dan 22,45 g ekstrak pekat etil asetat. Uji fitokimia menunjukkan bahwa keempat ekstrak positif mengandung flavonoid, namun ekstrak kloroform memiliki efek toksik yang paling tinggi. Pemisahan dengan kromatografi kolom menghasilkan 3 kelompok fraksi (Fa, Fb, dan Fc). Fraksi Fc positif flavonoid dan relatif murni serta paling toksik terhadap larva udang Artemia salina Leach dengan LC50 sebesar 63,10 ppm, isolat selanjutnya diidentifikasi dengan spektrofotometer IR serta UV-Vis. Hasil identifikasi menunjukkan isolat (Fc) merupakan senyawa golongan flavonoid jenis dihidroflavonol yang mempunyai gugus fungsi OH terikat, CH alifatik, C = O, C = C aromatik, C – O dan CH aromatik, serta terdapat gugus hidroksi pada atom C-3, C-5, dan C-7, serta mempunyai gugus orto dihidroksi pada cincin B dan memberikan serapan pada panjang gelombang (?max) 281,0 dan 315,0 nm.

ISOLASI DAN UJI ANTIRADIKAL BEBAS MINYAK ATSIRI PADA DAUN SIRIH (Piper betle Linn) SECARA SPEKTROSKOPI ULTRA VIOLET-TAMPAK I M. Oka Adi Parwata; Wiwik Susanah Rita; Raditya Yoga
Jurnal Kimia (Journal of Chemistry) Vol. 3, No. 1 Januari 2009
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Isolation, identification and free radical activities testing of essential oil from Piper betle Linn were carriedout. Around 13,0 mL (12, 37 gram) of yellow essential oil was obtained from 10,0 kg fresh leaves of Piper betleLinn. The oil was then partitioned with MeOH : H2O (7:3), n-hexane and chloroform.Free radical activities testing showed n-hexana fraction was the most active which can reduced 66,27 % ofDPPH for 5 minutes and 89,13 % in 60 minutes. GC-MS analysis revealed that there were 27 peaks. Based on thedata analysis there were only 9 compound, i.e. 4-metil(1-metiletil)-3-sikloheksen-1-ol, 1-metoksi-4(1-propenil)benzene, 4-(2-propenil)fenol/kavicol, 4-alilfenilacetate, Eugenol, Karyofilen, 3-alil-6-metoksifenilasetat, 4-alil-1,2-diasetoksibenzena dan dekahidro-4a-metil-1-metilen-7(1-metiletenil) naftalena.Based on peak’s intensity the oil were dominated by 4 compound i.e. 4-allyl phenyl acetate, 2 methoxy-4-(2 prophenil) fenol/eugenol, 3-allyl-6-methoxy phenyl acetate, 4-(2-prophenyl)-phenol / cavikol.

Co-Authors Abdul Rahim Adi Djona Silaen Ali Sabikin Frida R. Koreh Gede Widihati, Ida Ayu HERNINDYA, ANISA I Gusti Agung Gede Bawa I Gusti Ayu Kunti Sri Panca Dewi I Gusti Bagus Teguh Ananta I Kadek Pater Suteja I M, Sukadana I Made Adi Suardhyana I Made Dira Swantara I Made Oka Adi Parwata I Made Siaka I Nyoman Suarsana I Nyoman Suartha I W BUDIARSA SUYASA I Wayan Gede Gunawan I Wayan Suirta I. A. R. Astiti Asih Irdhawati Irdhawati Irvansyah Habibi Resaputra Iryanti Eka Suprihatin James Sibarani Kenanda, I Putu Bayu Mbatu, Rosanti Suryani Tince Ni Kadek Dyan Mustika Sri Wahyuni Ni Ketut Sinarsih Ni Ketut Sinarsih Ni Komang Ariati Ni Luh Putu Lilis Wirastiningsih Ni Luh Rustini Ni Luh Yuli Damayanti Ni Made Dwita Prasetyawati Ni Made Puspawati Ni Made Puspawati Ni Made Suaniti Ni Made Sukma Sanjiwani Ni Made Yuliari Ni Putu Eka Vinapriliani Ni Putu Widayanti Putu Prisanti Putri Utami Putu Puspita Sari Putu Sariningsih Raditya Yoga Rahayu, Riana Suastari Retno Kawuri Rr Anisa Hernindy Sinarsih, Ni Ketut Suharta, I Gede Yeyen Valent, Febryna Aurora WAHYU DWIDJANI S Wirajana, Nengah Yenni Ciawi Yohanes Armawan Sandi

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