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POTENTIAL OF CITRUS LEAF EXTRACT (Cymbopogon citratus (DC.) Stapf) AS AN ANTIBACTERIAL INHIBITORY of Staphylococcus epidermidis Karnelasatri, Karnelasatri; Cenwira, Wishia; Andareas, Pangeran; Santoso, Feronia Reni Cyrena
Jurnal Insan Farmasi Indonesia Vol 6 No 2 (2023): Jurnal Insan Farmasi Indonesia
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jifi.v6i2.1601

The concerns of antibiotic resistance have increased in recently years, this issue caused by long-term repeated use of antibiotics to treat bacterial illnesses. Lemongrass leaf (Cymbopogon citratus (DC.) Stapf) is one of the natural ingredients that can be used as an alternative antibacterial. The study objective is to determine the antibacterial activity of the ethanol extract of 70% cooking leaves against Staphylococcus epidermidis using diffusion methods. In addition, the Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) of extracts were determined using liquid dilution methods. The results of this study show that extract concentrations of 1,000,000 ppm have a barrier zone in the intermediate category when compared with the positive control, that is, clindamycin. Meanwhile, the MIC value obtained is 1250 ppm, while the MBC value is 5000 ppm. The results of statistical analysis showed that there were no significant differences in the inhibition zone diameter formed between the test concentrations.

Silver Nanoparticle Biosynthesis using Distimake vitifolius Extract for Enhancement of Antibacterial and Antioxidant Activity Tahya, Candra Yulius; Karnelasatri, Karnelasatri; Irawati, Wahyu; Munthe, Sri Wahyu Ningsih
Molekul Vol 19 No 2 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.2.10295

ABSTRACT. The biological method for silver nanoparticle synthesis emphasizes green synthesis, which is faster, environmentally safer, facile, energy-efficient, and cost-effective. It provides good alternatives to physical and chemical methods. In this research, we have synthesized silver nanoparticles using D. vitifolius ethanolic extract. The 38 natural compounds of flavonoid, phenolic or polyphenol compositions have been determined by LC-MS/MS-QTOF which is carried out using UNIFI software equipped with a mass spectrum library of active substances from natural ingredients from the Waters database. Silver nanoparticle (Dv-AgNP) was characterized by using TEM and SEM where the average diameter of nanoparticle was seen <100 nm and confirmed by PSA analysis that the Z-average is 62.73 ± 0.71 nm. EDS spectrum confirmed the silver element as a major element of the particles. PI value of Dv-AgNP is 0.557, while the zeta potential is -17.37 ± 1.53 mV. Dv-AgNP can inhibit the growth of P. aeruginosa (amoxicillin resistant) with an inhibition zone of 15.83 ± 2.36 mm, and K. pneumoniae with an inhibition zone is 10.03 ± 0.55 mm. These are moderate inhibitory activities. The antioxidant result of the Dv-AgNP suspension solution shows that the IC50 is 0.58 mM. The IC50 of the crude extract is 752 ppm and is categorized as very weak antioxidant activity. Keywords: Antibacterial; D. vitifolius; silver nanoparticle.

OPTIMASI DAN FORMULASI EKSTRAK ETANOL UMBI WORTEL (Daucus carota L.) SEBAGAI FACE SERUM SEBAGAI FACE SERUM [OPTIMIZATION AND FORMULATION OF CARROT ETHANOL EXTRACT (Daucus carota L.) AS A FACE SERUM] Karnelasatri, Karnelasatri; Trisina, Jessica; Purba, Rossa Maranatha
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol 7, No 2 (2023): NOVEMBER
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/jstfast.v7i2.7604

Carrot bulbs are known to contain secondary metabolite compounds that can act as antibacterials due to their activity, which suppresses the synthesis of nucleic acid, cytoplasmic membrane function, and energy metabolism in bacteria. This research was carried out using laboratory experimental methods through the determination of the plant, production of simplicia, extraction by ethanol (96% as solvent) with the maceration method, phytochemical screening, antibacterial assay, optimization of the base formula, formulation of carrot extract, physical evaluation of the products, and hedonic testing. The yield of 96% ethanol extract of the carrot bulbs was 53.29%, and the water content of the extract was 16.14%. Furthermore, the antibacterial activity of the extract (500.000 ppm) against Staphylococcus aureus showed an inhibitory zone of 5.1 ± 3.42 mm. The carrot extract serum formulas F1, F2, and F3 meet the organoleptic and homogeneity criteria; however, the F2 formula does not meet the pH and viscosity criteria. Based on the hedonic statistical test, no significant difference was found in each formula; nevertheless, based on the average value of the respondents, it was known that the F1 formula had the highest preference.Bahasa Indonesia Abstract:Umbi wortel dapat berperan sebagai antibakteri karena mengandung senyawa metabolit sekunder yang dapat menekan sintesis asam nukleat, fungsi membran sitoplasma, dan metabolisme energi pada bakteri. Penelitian ini dilakukan dengan metode eksperimental laboratorium yang dilakukan dengan determinasi tumbuhan, pembuatan simplisia serta ekstraksi dengan pelarut etanol 96% dengan metode maserasi, skrining fitokimia, uji antibakteri ekstrak, optimasi basis sediaan, formulasi sediaan, evaluasi fisik sediaan, dan uji hedonik sediaan. Rendemen ekstrak etanol 96% umbi wortel yang diperoleh adalah sebesar 53,29% dan kadar air ekstrak adalah 16,14%. Aktivitas antibakteri dari ekstrak (500.000 ppm) terhadap Staphylococcus aureus menunjukan zona hambat sebesar 5,9 ± 0,49 mm. Sediaan serum ekstrak umbi wortel F1, F2, dan F3 memenuhi syarat organolepik dan homogenitas, namun sediaan F2 tidak memenuhi syarat pH dan viskositas sediaan. Berdasarkan uji statistik hedonik sediaan, tidak ditemukan perbedaan signifikan pada tiap sediaan, namun berdasarkan nilai rata-rata responden diketahui bahwa sedian F1 menjadi preferensi tertinggi.

Perbandingan Sifat Feroelektrik Pada Sintesis Oksida Logam Aurivillius SrBi4Ti4O15 Empat Lapis Dengan Metode Hidrotermal Karnelasatri, Karnelasatri; Mikrianto, Edi
Indonesian Journal of Chemical Research Vol 6 No 2 (2019): Edisi Bulan Januari (Edition For January)
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2019.6-kar

Aurivillius compounds, SrBi4Ti4O15 (SBT four layers) have been synthesized by hydrothermal method at 200â„ƒ with variation of times 48 hours, 72 hours and 96 hours. Single phase of SrBi4Ti4O15 Aurivillius compounds obtained at the 200 Ëš C, 96 hours and the concentration of NaOH 3M. The Rietica program shown the index Miller result is 1 1 5 1 1 3, 0 2 0, 0 2 4, 2 0 8 2 2 0, 0 2 1 0, 3 1 6, 1 1 1 6 and the space group is A21am with the orthorhombic crystal system. Cell parameters are a = 5.5754 Ã…, b = 5.5456 Ã…, c = 41.3642. Ferroelectric properties show the value of remanent polarization Pr (+) and Pr (-) 4,61 C/cm2 and 2,75 C/cm2 and that is much lower than the value of remanent polarization of other material that have been synthesized at 240â„ƒ, 72 hours with the same method.

Adsorption of Indigo Carmine Dye and Copper Ion by Kaolin from Bangka Island Tahya, Candra Yulius; Cornelia, Melanie; Siregar, Tagor M.; Karnelasatri, Karnelasatri
Indonesian Journal of Chemical Research Vol 11 No 1 (2023): Edition for May 2023
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598//ijcr.2023.11-tah

Indigo Carmine (IC) is a synthetic dye that might promote irritations, vomiting, and diarrhea when orally consumed in high concentrations. Copper is a dangerous heavy metal that tends to accumulate in organisms' cells as a pollutant. Hyperproduction of IC and accumulation of copper in water could cause an environmental problem that must be adequately addressed. Kaolin is found abundant on Bangka Island. Based on XRD analysis, Kaolin Island from Bangka Island consists of two important minerals, Kaolinite and Rectorite. Kaolin has chemical and physical properties, which make it useful in several applications, including as an adsorbent. This study aims to evaluate how Kaolin can adsorb copper ion from acid wastewater (pH < 0.5) and determine natural Kaolin's ability to adsorb IC dye from an aqueous solution. Many factors, including mixing time and number of doses of Kaolin, influence Kaolin's ability to absorb IC. The longer the stirring time, the more IC is absorbed. The optimal temperature for IC adsorption is 25˚C. The optimal kaolin dose for IC adsorption is 20% (m/v). Copper concentration after application of Kaolin tends to decrease, with optimal at 25.09% (m/v) dosage for 19.23% copper removal.

Analysis of Hydroquinone in Face Whitening Cream by UV-Vis Spectrophotometry and GC-MS Spectrometry Karnelasatri, Karnelasatri; Tahya, Candra Yulius; Adila, Mahda Uli; Hardy, Junius; Munthe, Sri Wahyu Ningsih
Indonesian Journal of Chemical Research Vol 11 No 3 (2024): Edition for January 2024
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2024.11-kar

The analytical method to determine the compound content in both food and cosmetic products must be carried out carefully to ensure the validity of the analysis results. Hydroquinone has been widely found to be used as an active whitening agent in whitening cream. Technological advances have led to illegal whitening creams becoming more accessible online. This study aims to examine the hydroquinone content in the whitening samples cream purchased from e-commerce by various analytical methods, such as FeCl3 reagent, cerimetric titration, UV-Vis spectrophotometry, and GC-MS spectrometer compared to the positive control hydroquinone. The results of the hydroquinone content by cerimetric titration and UV-Vis spectrophotometry methods were calculated quantitatively. Furthermore, the result compared through statistical tests showed a consistent hydroquinone content for both methods. Contrariwise, the GC-MS method showed negative results due to differences in the extraction process and solvent solubility. Based on the result obtained, can be concluded that all samples of whitening cream did not meet the requirements of BPOM No. 23 of 2019.

Antibacterial Activity Assay on Escherichia coli of The Active Fraction Matoa Seeds (Pometia Pinnata J.R Forst & G. Forst.) Karnelasatri, Karnelasatri; Yacob, Iren Anggreni; Andareas, Pangeran; Santoso, Feronia Reni Cyrena; Novia, Jessica
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 10 No. 2 (2024): (October 2024)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24428744.2024.v10.i2.16283

One of the plants had been used as a medicinal plant in Papua is Pometia Pinnata J.R Forst & G. Forst or the Matoa plant. The aim of this study is to determine the antibacterial activity of the active fraction of Matoa seeds against Escherichia coli bacteria. The positive control and negative control used were ciprofloxacin and dimethyl sulfoxide, respectively. 96% ethanol solvent was used to extract the simplicia of Matoa seeds. Fractionation was carried out using n-hexane, ethyl acetate and water as solvents. The Kirby Bauer diffusion method was used in the antibacterial activity assay at concentrations of 100 mg/mL, 200 mg/mL, 300 mg/mL, 400 mg/mL, and 500 mg/mL. Overall, the inhibition zones obtained from the sequential concentrations above were 8.89 mm, 10.18 mm, 10.34 mm, 10.84 mm, and 11.76 mm respectively. Further, data analysis was performed with One Way ANOVA and the results showed significant differences between concentrations. Data analysis was then continued with the post hoc Tukey HSD test and a concentration of 500 mg/mL was concluded as the optimum concentration.

Meningkatkan Minat Belajar Siswa Selama Pandemi Covid-19 Melalui Bimbingan Belajar Online Friska J. Purba; Kelly Sinaga; Debora Suryani Sitinjak; Candra Yulius Tahya; Karnelasatri
International Journal of Community Service Learning Vol. 6 No. 2 (2022): May 2022
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijcsl.v6i2.46068

Pandemi COVID-19 telah merenggut kenyamanan siswa dalam mengikuti proses belajar mengajar. Kenyamanan belajar mengajar terenggut dikarenakan pemerintah menutup sekolah dan perguruan tinggi untuk menekan penyebaran virus COVID-19 yang kian merebak. Akibatnya, sistem pembelajaran harus diubah dari tatap muka (luring) menjadi tatap maya (daring). Pembelajaran jarak jauh (PJJ) yang dilakukan menyebabkan dampak negatif bagi siswa, salah satunya ialah penurunan minat dan semangat belajar. Hal ini terjadi dikarenakan kurangnya kemampuan guru dalam mengelola pembelajaran yang menarik dan bermakna. Berdasarkan permasalahan tersebut, maka Program Studi Pendidikan Kimia bekerja sama dengan jemaat GKPS Tangerang untuk membuat Pos Bimbingan Belajar Online Gratis yang diberi nama Pos Diakonia untuk membantu anak-anak, khususnya anak-anak jemaat GKPS dalam memahami materi sekolah dan meningkatkan minat dan semangat belajar siswa. Kegiatan ini berlangsung selama 8 minggu. Dari survei yang dilakukan kepada 83 tutee, hanya 44 yang memberikan respon. Dari respon tersebut didapatkan bahwa setelah mengikuti bimbel online hampir semua tutee merasakan peningkatan pemahaman, minat dan semangat belajar. Namun sayangnya, masih banyak juga tutee yang mengalami kesulitan dan minat belajar yang kurang bertumbuh.

OPTIMASI DAN FORMULASI EKSTRAK ETANOL UMBI WORTEL (Daucus carota L.) SEBAGAI FACE SERUM SEBAGAI FACE SERUM [OPTIMIZATION AND FORMULATION OF CARROT ETHANOL EXTRACT (Daucus carota L.) AS A FACE SERUM] Karnelasatri, Karnelasatri; Trisina, Jessica; Purba, Rossa Maranatha
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 7 No. 1 (2023): NOVEMBER
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/jstfast.v7i2.7604

Carrot bulbs are known to contain secondary metabolite compounds that can act as antibacterials due to their activity, which suppresses the synthesis of nucleic acid, cytoplasmic membrane function, and energy metabolism in bacteria. This research was carried out using laboratory experimental methods through the determination of the plant, production of simplicia, extraction by ethanol (96% as solvent) with the maceration method, phytochemical screening, antibacterial assay, optimization of the base formula, formulation of carrot extract, physical evaluation of the products, and hedonic testing. The yield of 96% ethanol extract of the carrot bulbs was 53.29%, and the water content of the extract was 16.14%. Furthermore, the antibacterial activity of the extract (500.000 ppm) against Staphylococcus aureus showed an inhibitory zone of 5.1 ± 3.42 mm. The carrot extract serum formulas F1, F2, and F3 meet the organoleptic and homogeneity criteria; however, the F2 formula does not meet the pH and viscosity criteria. Based on the hedonic statistical test, no significant difference was found in each formula; nevertheless, based on the average value of the respondents, it was known that the F1 formula had the highest preference.Bahasa Indonesia Abstract:Umbi wortel dapat berperan sebagai antibakteri karena mengandung senyawa metabolit sekunder yang dapat menekan sintesis asam nukleat, fungsi membran sitoplasma, dan metabolisme energi pada bakteri. Penelitian ini dilakukan dengan metode eksperimental laboratorium yang dilakukan dengan determinasi tumbuhan, pembuatan simplisia serta ekstraksi dengan pelarut etanol 96% dengan metode maserasi, skrining fitokimia, uji antibakteri ekstrak, optimasi basis sediaan, formulasi sediaan, evaluasi fisik sediaan, dan uji hedonik sediaan. Rendemen ekstrak etanol 96% umbi wortel yang diperoleh adalah sebesar 53,29% dan kadar air ekstrak adalah 16,14%. Aktivitas antibakteri dari ekstrak (500.000 ppm) terhadap Staphylococcus aureus menunjukan zona hambat sebesar 5,9 ± 0,49 mm. Sediaan serum ekstrak umbi wortel F1, F2, dan F3 memenuhi syarat organolepik dan homogenitas, namun sediaan F2 tidak memenuhi syarat pH dan viskositas sediaan. Berdasarkan uji statistik hedonik sediaan, tidak ditemukan perbedaan signifikan pada tiap sediaan, namun berdasarkan nilai rata-rata responden diketahui bahwa sedian F1 menjadi preferensi tertinggi.

MOLECULAR DOCKING OF ANTIBACTERIAL ACTIVITY OF HEXAHYDRO-1,2,3-TRIAZINE DERIVATIVES Tahya, Candra Yulius; Irawati, Wahyu; Karnelasatri, Karnelasatri
Walisongo Journal of Chemistry Vol. 8 No. 2 (2025): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology UIN Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v8i2.26159

Hexahydro-1,3,5-triazine and its derivatives have piqued the interest of numerous researchers due to its strong biological characteristics, which include antibacterial, antimalarial, fungicidal, antiviral, anticancer, antimicrobial, antiamoebic, anti-inflammatory, and antitubercular effects. The antibacterial activity test in this work was performed using molecular docking on 16 hexahydro-1,3,5-triazine derivative compounds and three drug molecule controls that were selected from the PDB database and targeted Penicillin binding protein 2A (PDB ID: 1VQQ). For docking, Pyrx v.1.1's AutodockVina integration was utilized. Docking utilizing the targeted docking strategy was performed using a specific gridbox that was chosen based on the location of significant amino acids and pharmacological controls. The drug potential of a chemical under test is described by the Pa (Probability to be Active) value. The results of the Pa value analysis showed that several hexahydro-1,3,5-triazine derivative compounds had a higher/better average Pa value compared to the control drugs Ceftaroline and Ceftobiprole. Based on the Lipinski rule of five, the analysis's findings indicated that six out of the sixteen test compounds had good drug-likeness features; however, according to Pfizer, every compound possessed these qualities. Nearly all of the drugs exhibited good pharmacokinetics, according to the ADMET study. According to molecular docking results, 13 out of 16 synthesized compounds generally exhibit a high affinity for the PBP2a target protein. Furthermore, four compounds had a higher affinity than the ceftobiprole drug control when compared to the three drug controls. Synthetic compounds, particularly compounds 7, 16, 15, and 1, have the potential to be used as antibacterial therapies, according to the results of QSAR, ADMET, and docking research.

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