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All Journal Pharmaciana: Jurnal Kefarmasian Jurnal Kimia Riset Jurnal Ilmiah Kedokteran Wijaya Kusuma THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Ilmiah Soulmath : Jurnal Edukasi Pendidikan Matematika Jurnal Mandala Pharmacon Indonesia Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Medical Technology and Public Health Journal Sains dan Matematika Medicra (Journal of Medical Laboratory Science/Technology) Jurnal Abdimas Berdaya : Jurnal Pembelajaran, Pemberdayaan dan Pengabdian Masyarakat Jurnal Abdimas Kartika Wijayakusuma Indonesian Journal of Innovation Studies Procedia of Social Sciences and Humanities Prosiding SEMNAS INOTEK (Seminar Nasional Inovasi Teknologi) Oshada Jurnal Teknologi Laboratorium Medik Borneo Manajemen Pelayanan Kesehatan Indonesian Journal Health Science and Medicine Jurnal Analis Kesehatan Kendari
Chylen Setiyo Rini
Universitas Muhammadiyah Sidoarjo
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Journal : Medicra (Journal of Medical Laboratory Science/Technology)

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The Detection of TB Lungs with Microscopic and the Rapid Molecular Test Methods Fifi Isti Tamtyas; Chylen Setiyo Rini
Medicra (Journal of Medical Laboratory Science/Technology) Vol 3 No 1 (2020): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v3i1.650

Abstract

Mycobacterium tuberculosis is a cause of tuberculosis (TB ) disease. In this research Mycobacterium tuberculosis can be detected using the Microscopic and Real-Time PCR. This research aims are to know the difference in the results of the examination Mycobacterium tuberculosis with microscopic method and Real-Time PCR. This research used an experimental research design and was tested using the Chi Square test. This results showed at significant difference (p=0.000) detection of pulmonary TB disease by microscopic and Real-Time PCR methods
The Potential of Mango (Mangifera infica L.) Peel of Apple Varieties By Infusion And Maceration In Inhibiting Pseudomonas aeruginosa And Propionibacterium acnes: Potensi Kulit Mangga (Mangifera infica L.) Varietas Apel Secara Infusa Dan Maserasi Dalam Menghambat Bakteri Pseudomonas aeruginosa dan Propionibacterium acnes Rahmawati, Vifin Putri; Rini, Chylen Setiyo
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 4 No. 1 (2021): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i1.904

Abstract

Plants have many chemical components. The use of natural ingredients as an alternative treatment in dealing with diseases, especially acne. One of them is mango (Mangifera indica L.) varieties of apples obtained at the Larangan Main Market in Sidoarjo. This study aims to determine the potential of infusion and maceration of mango skin varieties in inhibiting Pseudomonas aeruginosa and Propionibactrium acne at various concentrations. This antibacterial potential test was carried out using the diffusion method of the wells. The antibacterial potential is characterized by the formation of a clear zone around the well called the inhibition zone. This study uses 10 concentrations namely 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% and Clindamycin as positive control and aquades as negative control. Based on the results of the Two Way ANOVA test data obtained were not normally distributed, therefore a comparison test was performed using the Kruskal-Wallis test with a sign value (α <0.05). This showed that there were significant differences in the use of various concentrations. The maceration extract concentration of 100% is the best concentration to form a zone of inhibition against P. aeruginosa of 17.9 mm and P. acne bacteria of 13.2 mm. The results of the infusion extract concentration did not form inhibitory zones in both of P. aeruginosa and P. acnes.
Effectiveness of The Antibacterial Activity on Orthosiphon aristatus Leaves Extract Against Proteus mirabilis and Staphylococcus saprophyticus: Uji Efektivitas Antibakteri Ekstrak Daun Kumis Kucing (Orthosiphon aristatus) Terhadap Bakteri Proteus mirabilis dan Staphylococcus saprophyticus Nisak, Khairun; Rini, Chylen Setiyo
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 4 No. 2 (2021): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i2.1582

Abstract

Orthosiphon aristatus is traditional medicinal plants. Orthosiphon aristatus leaves contain alkaloid, saponin, steroid, triterpenoid, and phenolic which are antibacterial compounds. Proteus mirabilis and Staphylococcus saprophyticus can cause urinary tract infections. This study aims to determine the optimum concentration of Orthosiphon aristatus leaves extract against Proteus mirabilis and Staphylococcus saprophyticus. This research is an experimental study. This research was carried out in the Bacteriology laboratory of the Muhammadiyah University of Sidoarjo. Antibacterial activity test of Orthosiphon aristatus leaves extract used the disc diffusion method at a concentration of 25%, 50%, 75%, 100%. This test is performed 3 times. Measurement of the diameter of the zone of inhibition is measured using a caliper. The data obtained were tested statistically with Anova with a significant level of 0.05 (5%). The results showed that the Orthosiphon aristatus leaves at a concentration of 25%, 50%, 75%, 100% had a significant effect on Proteus mirabilis with an average of 6.22 mm, 9.36 mm, and 15.55 mm, 21.22 mm and Staphylococcus saprophyticus with a mean of 5.4 mm, 8.8 mm, 14.53 mm, and 20.71 mm.
Efektivitas Kunyit (Curcuma longa Linn) terhadap Esherichia coli dan Bacillus subtilis: Effectiveness of Turmeric (Curcuma longa Linn) against Esherichia coli and Bacillus subtilis Rini, Chylen Setiyo; Rohmah, Jamilatur; Widyaningrum, Leni Yuroh
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 1 No. 1 (2018): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v1i1.1546

Abstract

Herbal plants that serve as a drug, one of which is turmeric that has a role as an antioxidant, antimicrobial, anti-cancer, digestive disorders. Because it contains compounds curcumin and essential oils. The purpose of this research is to know the optimal concentration of dry extract of turmeric rhizome (Curcuma longa L) in inhibiting the growth of E. coli and Bacillus subtilis. This research method using diffusion agar Kirby Bauer. In this study, turmeric extract at 15% concentration had antibacterial efficacy against Bacillus subtilis (0,7 mm) greater than Escherichia coli (0,63 mm).
Kontaminasi Escherichia coli pada Makanan Jajanan di Kantin Universitas Muhammadiyah Sidoarjo: Contamination of Escherichia coli in Snacks at the Canteen of Muhammadiyah University of Sidoarjo Rohmah, Jamilatur; Rini, Chylen Setiyo; Cholifah, Siti
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 1 No. 1 (2018): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v1i1.1521

Abstract

One of the places to get food is in the cafeteria. Food made in the canteen can be a cause of foodborne desesase and food poisoning if not properly disposed. The objective was to analyse the quality of food microbiology on canteen food in campus 1, 2, and 4 University Muhammadiyah of Sidoarjo. This study applied the descriptive observational which cross sectional design in canteen food stalls and sampling technique which is total sampling. Tests of canteen food samples were performed using Total Plate Count (TPC). A total of 35 samples of canteen foods from the campus canteen. The results show that positive E.coli contamination on canteen food (canteen campus 1, 2, and 4) was 25 (71.43%). There is a significant difference from the burden of microbial samples of canteen food. This research was conducted to find out personal hygiene and environment, and the cleanliness of the campus should be improved and maintained.
Identifikasi Eschericia coli dan Salmonella sp. pada Air Kolam Renang Candi Pari: Identification of Eschericia coli and Salmonella sp. on the Pari Temple Swimming Pool Water Khakim, Lukmanul; Rini, Chylen Setiyo
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 1 No. 2 (2018): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v1i2.1491

Abstract

Incorrect treatment of swimming pool can contaminate the water with bacteria. Common bacteria in water are Escherichia coli and Salmonella sp, both of which may infect the users of the swimming pool. Infection causes diarrhea and high fever on humans. The aim of this research is to investigate the contamination of Escherichia coli and Salmonella sp at Candi pari swimming pool. Research design used in this research is the development of EMB and SSA selective medium, gram coloring test, and biochemical test to confirm the presence of Escherichia coli and Salmonella sp. Resultsfrom this research is the growth of Escherichia coli and Salmonella sp from 1 ml sample which were inoculated on selective medium. Conclusion from this research is from 2 samples taken from Candi Pari swimming pool, both are positively contaminated with Escherichia coli and Salmonella sp.
Efektivitas Jeruk Lemon (Citrus limon Linn) terhadap Staphylococcus epidermidis: Effectiveness of Lemon (Citrus limon Linn) against Staphylococcus epidermidis Hartin, Ellyce; Rini, Chylen Setiyo
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 1 (2019): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i1.1617

Abstract

Acne or acne vulgaris is the one of the skin diseases that often appear in adolescents and adults. Acne is often associated with the condition of the body, either in stress condition because of sad or being happy. Acne can be caused by genetic, cosmetic, and bacterial factors. Bacteria that can cause swelling diseases is Staphylococcus epidermidis. For overcoming acne, people can use traditional medicine such as lemon juice (Citruslimon Linn). The main composition that exists in lemon juice (Citrus limon Linn) is citric acid that acts as an antimicrobial against bacteria present in acne. This study aims to determine the influence of lemon juice lime juice (Citrus limon Linn) against Staphylococcus epidermidis. This research is an experimental laboratory that use disc disk method or Kirby bauer method with concentration 0%, 12,5%, 25%, 50% and 100%. The result of the study shows that lemon lime juice (Citrus limon Linn) at 25% concentration already have a transparent zone around the paper disc about 12.1 mm and have low effectivity to secure Staphylococcus epidermidis. The high concentration of lemon juice will increase the inhibitory power produced by lemon juice (Citrus limon Linn).
Identifikasi Aspergillus flavus pada Kue Pia yang Di Jual Di Dusun Warurejo Kabupaten Pasuruan: Identification of Aspergillus flavus on Pia Cakes Sold in Warurejo Hamlet, Pasuruan Regency Lindawati, Sari; Rini, Chylen Setiyo
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 2 No. 2 (2019): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v2i2.1618

Abstract

Warurejo village that located in Pasuruan district is the center of pia cake business expecially green beans pia. Pia cake is a cake that can’t durable cause by microorganism that come from the less clear and wraping in production. It makes contamination of mycotoxins from spesies of the fungus is Aspergillus flavus. Aspergillus flavus can produce a toxin metabolite compund that is aflatoxin. If aflatoxin is continuous consumtion will cause liver cancer. This research aim to determine presence of Aspergillus flavus on pia cake that produce by center of pia cake business in Warurejo village. It will be research with storage time of 0, 5 and 10 days. This study is descriptive, the number of sample taken randomly as many as 30 samples from 33 stores. Research this is obtained by macroscopically and microscopically. In macroscopically colonis that, look yellowish greeen and on the bottom is yellow to brown while microscopically rounded vesicles and conidi are round. 73% of Aspergillus flavus has positive contaminated fungus in 0 days and 5 days. 97% positive was Aspergillus flavus with 10 days storage time. In day 10 found more Aspergillus flavus fungus that on days 0 and 5 days.
Effectiveness Test of Okra Fruit (Abelmoschus esculentus) Extract on The Growth of Trichophyton rubrum: Uji Efektivitas Ekstrak Buah Okra (Abelmoschus esculentus) Terhadap Pertumbuhan Jamur Trichophyton rubrum Kimberly, Bella Tasya; Rini, Chylen Setiyo
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 5 No. 2 (2022): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v5i2.1647

Abstract

Dermatophytosis is an infectious disease of the skin caused by one of the pathogenic agents of the fungus Trichophyton. Trichophyton rubrum is a dermatophyte fungus that is included in the anthropophilic classification, where transmission occurs through direct human-to-human contact. This experiment was conducted to determine the effectiveness of giving okra fruit extract (Abelmoschus esculentus) to the growth of Trichophyton rubrum using well method, media that is used to do that is SDA (Sabouraud Dextrose Agar) with various concentrations of 25 %, 50%, 75%, 100%. Based on the results of phytochemical tests, the active compounds contained in okra fruits extract are alkaloids, flavonoids, saponins, steroid, triterpenoids, phenolics and tannins. Variations in concentration of okra fruit extract and negative control tested against Trichophyton rubrum did not show the formation of inhibition zones in the media. Meanwhile for positive control using 2% ketoconazole, an inhibition zone 35 mm was formed. This research showed that Trichophyton rubrum was resistant to okra fruit extract.
Acute Toxicity Test Against Mice (Mus Musculus) On The Lempuyang Gajah Extract (Zingiber Zerumbet (L.) Roscoe Ex Sm.) With Sgot Sgpt Parameters: Uji Toksisitas Akut Terhadap Mencit (Mus Musculus) Pada Ekstrak Lempuyang Gajah (Zingiber Zerumbet (L.) Roscoe Ex Sm.) Dengan Parameter SGOT SGPT Sofi Rahayu, Bella; Rohmah, Jamilatur; Rini, Chylen Setiyo; Puspitasari
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 7 No. 1 (2024): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v7i1.1656

Abstract

Lempuyang Gajah (Zingiber zerumbet (L.) Roscoe ex Sm) is a medicinal plant that has many benefits in the medicinal industry. Useful as a tonic, external medicine, anti-seizure and appetite enhancer. This study aims to determine the toxicity of the lempuyang gajah. This research uses laboratory experimental methods. The lempuyang gajah plant was obtained in the village of Terungkulon, Krian. The initial stage was the administration of lempuyang gajah extract with various doses of 4000, 5000 and 6000 mg/kg BB orally, consisting of 5 treatment groups. Parameters observed, changes in toxic symptoms, macroscopic examination and parameters of SGOT, SGPT. The results were analyzed using the One Way ANOVA test. The results of the study after giving the extract did not cause death so it was included in the non-toxic category, there were several symptoms such as weakness and seizures. The levels of SGOT, SGPT showed normal average results, statistically there was no effect (p > 0.05). Macroscopic results showed no changes or abnormalities in the liver. So that the ethanol extract of lempuyang gajah can be said to be safe for humans.
Co-Authors AA Sudharmawan, AA Absari, Eki Puji Ade Teti Vani Aini, Aqiliyanti Nur Aisyah Putri, Aprilia Akbar, Aulia Rizky Fadhillah al-akbar, Wildan Alhafiz, Rafaiz Filaili Ambarani, Putri Yuli Anggraeni, Rifka Wahyu Anugraheni Anugraheni Azzahra, Aulia Bayu Hari Prasojo Devi Arvindani Masyitha Fifi Isti Tamtyas Fitria Ayu Prayekti, Rahmatika Fitria Eka Wulandari Galuh Ratmana Hanum Hartin, Ellyce Huesein, Ida Agustini Saidi Imamah, Siti Aliyatul Jamilatur Rohmah Jamilatur Rohmah Jannah, Vita Wardah Nur Juliana, Tiara Karlia Hedit Tiana Kimberly, Bella Tasya Liany, Ayundah Novie Lidyaningrum, Galuh Ajeng Lindawati, Sari Liyaajul, Pratasyah Makhnunah, Makhnunah Miftahul Mushlih MUFIDAH, LAILATUL Mustar Nisak, Khairun Nugroho, Rhenald Aditya NUR EFENDI Nuril Lutvi Azizah Oktaruliawan, Veronika Ony Wibriyono Angkejaya Puspitasari Puspitasari Puspitasari Puspitasari Puspitasari Putri, Wafiq Zahro Rahmawati, Vifin Putri Ribangun Bamban Jakaria Saputra, Eduwardo Roy Sari, Azizah Krismonita Setyaputri, Nabila Hani SITI CHOLIFAH Sofi Rahayu, Bella Sukmawati Sukmawati Tis’iyyah , Siti Nur Maghfiroh Tri Chahyani Rahmawati Putri Wahyudi, Eko Bambang Widyaningrum, Leni Yuroh Yanik Purwanti Yeninastiti, Reny Zulfida Ainindiyah Putri Purwanto