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All Journal Jurnal Veteriner Aceh Journal of Animal Science Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science
Tyasningsih, Wiwiek
Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, PSDKU Banyuwangi, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Social Sciences Veterinary Other

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Journal : Aceh Journal of Animal Science

 1 
Molecular characterization of Pasteurella multocida pfhaB1 gene fragment from buffalo and cattle isolates from Nusa Tenggara Timur Indonesia Ine Karni; Didik Handijatno; Lucia Tri Suwanti; Kusnoto Kusnoto; Jola Rahmahani; Wiwiek Tyasningsih
Aceh Journal of Animal Science Vol 4, No 2: December 2019
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.4.2.13581

Abstract

Almost all regions in Nusa Tenggara Timur East Nusa Tenggara (NTT) Province Indonesia are endemic areas of Haemorragic Septicaemia (HS), which is caused by Pasteurella multocida  (P. multocida ) Serotypes B: 2. The fragment  pfhaB1gene is one of the virulence factors of P. multocida.The objective of this study was to determaine the phylogenetic, homology of P. multocidapfhaB1gene fragment of isolatedfrom Buffalo and Cattle in NTT. The P. multocida isolateswere re-cultured and further microscopic examined the biochemical tests, PCR, sequencing, homology, and phylogenetic relatedness test. P. multocida was observed as gram negative, coccobacilus, no growth on MacConkey Agar, does not produce H2S and gas, nonmotile and indole positive, does not produce urease enzymes, does not use citrate as a carbon source, does not ferment maltose and lactose but it does ferment glucose, sucrose and mannitol. ThepfhaB1gene fragmentfrom buffalo and cattle NTT isolates and also Katha strain vaccine showed DNA band 506 bp. P.multocida isolates from buffalo and cattle in NTT have 91% - 99% score homology with the comparative isolate. The isolate P. multocida from buffalo and cattle in NTT are in one cluster and their phylogenetic relatedness is close to isolates from Iran and India. It is concluded that the  pfhaB1gene fragmentof P. multocida from buffalo and cattle isolates have phylogenetic relatedness close and homolog with the other comparative isolates.    Keywords: Haemorrhagic Septicaemia; Nusa Tenggara Timur; Pasteurella multocida;  pfhaB1gene
Blastocystis sp. : Evaluation of polyclonal antibody prepared from crude protein for serological diagnosis using Rabbit serum Septian Hakim Susantoputro; Lucia Tri Suwanti; Rahadju Ernawati; Mufasirin Mufasirin; Setiawan Koesdarto; Wiwiek Tyasningsih; Heni Puspitasari
Aceh Journal of Animal Science Vol 5, No 2 (2020): Desember 2020
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.5.2.16780

Abstract

The diagnosis of Blastocystis infection is still based on the clinical sign which is not specific and there is no available serologic test for it. This study aimed to evaluate the polyclonal antibody prepared form crude protein of Blastocystis for the development of the Blastocystis serological test. Crude protein was extracted from the yeast of Blastocystis sp, then inoculated into rabbits to produce the antibody of crude protein. The serum of rabbits would be collected before and after immunization to compare the antibody titer. The profile of crude protein was analyzed using SDS-Page. The rabbit serum was analyzed using ELISA and Western Blot. The SDS-Page result showed bands in 100 kDa, 90 kDa, 70 kDa, 60 kDa, 58 kDa, 50 kDa, 40 kDa, 35 kDa, 30 kDa and 27 kDa. The ELISA assay showed that there was an increase in antibody titer of crude protein after immunization. Western Blot showed that three proteins (30 kDa, 40 kDa and 50 kDa) having immunogenicity characteristic. It is concluded that protein 30 kDa, 40 kDa and 50 kDa prepared from the crude protein of Blastocystis sp. can be used for developing a serologic test for Blastocystis infection. Keywords: Blastocystis sp, Crude Protein, Polyclonal Antibody  .
Co-Authors A. T. Soelih Estoepangestie Arum Maula Hidayah Boedi Setiawan Didik Handijatno Didik Handijatno Elisa Herina Dimariwu Hani Plumeriastuti Heni Puspitasari Ine Karni Kusnoto Kusnoto Lucia Tri Suwanti, Lucia Tri Mufasirin Mustofa Helmi Effendi Rahadju Ernawati Rahaju Ernawati Rahmahani, Jola Retno Wulansari Septian Hakim Susantoputro Setiawan Koesdarto Soeharsono Soeharsono Wiwik Misaco Yuniarti Yonatan Dimascahyo Budianto