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All Journal Prosiding BERITA BIOLOGI Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal AGROSCIENCE Molekul: Jurnal Ilmiah Kimia Scripta Biologica Budimas : Jurnal Pengabdian Masyarakat BioEksakta : Jurnal Ilmiah Biologi Unsoed Buitenzorg: Journal of Tropical Science

Alice Yuniaty

Fakultas Biologi Universitas Jenderal Soedirman

Author-ID : 3698256

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Social Sciences Veterinary Other

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ANALISIS RAPD KECIPIR POLONG PANJANG Psophocarpus tetragonolobus (L.) DC HASIL MUTASI IRADIASI SINAR GAMMA Arini Maesaroh; Adi Amurwanto; Alice Yuniaty
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Winged bean [Psophocarpus tetragonolobus (L.) DC] is a tropical plant that has some benefits and is very suitable to be cultivated in Indonesia. Study about diversity of winged bean is very important to support the future development of winged bean. Increased genetic diversity can be done through mutation. Gamma ray is often used for inducting mutations. One of way to observe genetic diversity resulted by molecular mutation is using RAPD method. The purpose of this study was to identify the genetic diversity of winged bean resulted by gamma ray irradiation. The method used in this study was an experiment by using RAPD technique. Samples used were plant leaves that had been induced by gamma ray with a wavelength of 20 Gy, 25 Gy, and control plants that were not induced by gamma ray. RAPD were done by ten primers that were OPA 9, OPA 10, OPA 13, OPA 18, OPB 2, 3 OPB, OPB 6, 7 OPB, OPB 10, and OPB 11. Data were analyzed using GenAlex 6.1 Program. The percentage of polymorphic loci of winged bean population control was 47,54%, while in the 20 and 25 Gy treatment were 62,30% and 54,10%, respectively. The values of genetic variation based on the calculation of allele frequencies were 0,236; 0,202 and 0,194 for treatment of 20 Gy, 25 Gy and for control plants, respectively. Meanwhile, the value of genetic distance ranged from 0.08 to 0.32.

VARIASI SEKUENS DNA YANG DIAMPLIFIKASI MENGGUNAKAN PRIMER atpB-rbcL PADA BEBERAPA KULTIVAR KACANG TANAH Yani Yuliani; Alice Yuniaty; Agus Hery Susanto
Scripta Biologica Vol 4, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Peanut is one of food crops commonly consumed in Indonesia. This species comprises several cultivars such as Kancil, Bison, Jerapah, Talam, and Tuban, each of which has its individual advantages and disadvantages. The vast variation among peanut cultivars leads to the need of study on genetic diversity and relationship among them using particular molecular marker. This study aims to see whether variation on DNA sequences among some peanut cultivars amplified with atpB-rbcL primers exists or not and to know the relationship among the cultivars based on the amplicon sequences. The method involves some sequential steps, i.e. genomic DNA isolation using CTAB protocol, amplification of DNA sequence using atpB-rbcL primers and sequencing of the amplification products. Data on sequences were edited manually using Bioedit version 7.0.4.1. Sequence alignment was performed using ClustalW, which is also implemented in Bioedit version 7.0.4.1. Arlequin 2.0 was used to calculate nucleotide diversity p. Phylogenetic analysis was performed using Maximum Parsimony in MEGA 5.0. The results showed that considerably high variation in DNA sequences of some peanut cultivars amplified with atpB-rbcL primers are observed. On the other hands, very close genetic relationship among cultivars is found.

Co-Authors Adi Amurwanto Adi Amurwanto Agus Hery Susanto Agus Hery Susanto AGUS HERY SUSANTO Arini Maesaroh Dani Satyawan Eliada Herwiyanti ENDANG SRIMURNI KUSMINTARSIH Esa Kurniawan Fajar Wahyu Pribadi Gabriella Ashari Tanudharma Hartanto Nugroho Helmanto, Hendra Ikhsan - Matondang Juwarno Juwarno Juwarno, Juwarno Kusumanegara, Kusumawaty Mahat Magandhi Maryanti Sitio, Risma Leonny Murni Dwiati Nurtjahjo Dwi Sasongko Polosoro, Aqwin Ramadani, Didi Retno Mayangsari Ridzqya, Salsabila Al Alya Saefuddin Aziz Sisunandar . Suryadi Suryadi Tanudharma, Gabriella Ashari Toto Hadiarto triani hardiyati Triani Hardiyati Wening Enggarini Yani Yuliani

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