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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Silvikultur Tropika (JST) MEDIA KONSERVASI Jurnal Sumberdaya Hayati Jurnal Bioteknologi & Biosains Indonesia (JBBI) JURNAL AGROHITA Jurnal Agroplasma Jurnal Hutan Tropika Bioscientist : Jurnal Ilmiah Biologi Journal of Life Science and Technology
Edhi Sandra
Departemen Konservasi Sumberdaya Hutan Dan Ekowisata, Fakultas Kehutanan Dan Lingkungan, Universitas IPB
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Social Sciences Other

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Journal : Jurnal Silvikultur Tropika (JST)

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Keberhasilan Inisiasi Eksplan KEBERHASILAN INISIASI EKSPLAN TUNAS DAN DAUN GMELINA (Gmelina arborea L.) DENGAN PENERAPAN BERBAGAI METODE STERILISASI Wulandari, Arum Sekar; Sandra, Edhi; Dian Kirani, Annisa
Journal of Tropical Silviculture Vol. 16 No. 02 (2025): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.16.02.107-115

Abstract

Gmelina is a fast growing species that has high sufficient economy value and useful as medicinal plant. Propagation of gmelina by tissue culture has not been widely applied in Indonesia. This study aims to analize the effect of explant types and sterilization methods on the success of gmelina initiation. Treatments used in this study are explant types (apical shoot and leaf) and four sterilization methods (B1-B4) using detergent, tween 80, fungicide, bactericide, NaOCl, and HgCl2. All sterilization methods can produce 6‒19% sterile gmelina shoot culture, but has not succeded producing sterile gmelina leaf culture. Contamination by fungi and bacteria is the main cause of failed gmelina shoot and leaf culture initiation. In general, sterilization methods used in this study has succeeded in eliminating microbes on the explant’s surface, but has not succeeded in eliminating microbes inside the explant.
Co-Authors . Siswoyo Ade Tiyan Handayani Agus Hikmat Anggit Haryoso Ani Lestari Anita Widya Karyaningtyas Arum Sekar Wulandari Daru Mulyono Daru Mulyono Diah Ratnadewi Dian Kirani, Annisa Ervizal A. M. Zuhud Ervizal A.M. Zuhud Fauzan, Yusuf Sigit Ahmad Fauzan, Yusuf Sigit Ahmad Hanik Faizah Hardiyanti Pebrian, Rini Heru Sugito Hilda Wijaya Istomo . Lilik Listiani M. Rafi Michael Michael Mufti Abdillah Muhammad Syafii Nadiya Dwi Rahayu Nampiah Sukarno Nurcahyo Widyodaru Nurcahyo Widyodaru Nurul Kamila Primadika Al Manar Rahayu, Nadiya Dwi Risna Rita Kartika Sari RUSMILAH SUSENO Setya Kurniawan Sofi Mursidawati Sofi Mursidawati Sri Listiyowati Suharli, Lili Sulistyo Sidik Purnomo Syafitri Hidayati Vianti Vianti Win Ariga Mansur Malonga Windi Widia Nengsih Yana, Kholika YANTO SANTOSA Yusuf Sigit Ahmad Fauzan