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All Journal JBIO: jurnal biosains (the journal of biosciences) REKAYASA BIOTROPIC The Journal of Tropical Biology Jurnal Biologi Tropis Al-Ard: Jurnal Teknik Lingkungan Journal of Innovation in Educational and Cultural Research Bioscientist : Jurnal Ilmiah Biologi

Hanik Faizah

Biology Deparment, Faculty Sains And Technology, UIN Sunan Ampel Surabaya, Dr. Ir, H. Seokarno Street Number 682, Surabaya, Indonesia

Author-ID : 2601910

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology Physics Social Sciences

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Dogs (Canis lupus familiaris) as Virus Carrier in Indonesia Moch Irfan Hadi; Muhammad Yusuf Alamudi; Mei Lina Fitri Kumalasari; Sri Hidayati; Tatag Bagus Prakarsa; Eva Agustina; Muhamad Ratodi; Misbakhul Munir; Eko Teguh Pribadi; Hanik Faizah
Biotropic : The Journal of Tropical Biology Vol. 2 No. 2 (2018): Biotropic, Volume 2, Nomor 2, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

A Virus is an individual that cannot be described as an animal or a plant. If animals and plants contain two nucleic acids (DNA and RNA), on the contrary, the virus only has one of them. These nucleic acids can stimulate a complete virus replication cycle. The virus can only replicate and live on a living host if the host is finally dead, then the virus will move on the cells that are still alive. The virus has genetic material which is a protective protein coat called a capsid. Viruses can infect various varieties of organisms, both eukaryotes (animals, plants, protists, and fungi) and prokaryotes (bacteria and archaea). The Virus infects bacteria known as bacteriophage (phage). The Virus can cause serious diseases for humans such as AIDS, HIV, rabies, etc. Dogs belonging to Canidae family are the sibling of wolves, foxes and raccoon dogs. Among all members of Canidae, dogs have the most closely related to wolves which are the ancestors of dogs. The Canidae family generally has a small elongated body, sharp ear and muzzle, sharp smelling, can run fast and can swim. Dogs are human best friends. While taking care of the dogs, they can be attacked by various diseases. The closeness of the relationship between humans and dogs raises the potential for disease transmission, especially zoonosis and pandemics viruses. Keywords: Canis lupus familiaris, Carier, Influenza, virus

Uji Cemaran Mikroba Pada Air Yang Digunakan Untuk Mencuci Peralatan Makan Oleh Pedagang Kaki Lima di Sekitar UIN Sunan Ampel Surabaya Fahimatul Ula; Misbakhul Munir; Hanik Faizah
Biotropic : The Journal of Tropical Biology Vol. 5 No. 2 (2021): Biotropic, Volume 5 Nomor 2, 2021
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2021.5.2.101-115

The condition of eating places such as street vendors will determine the level of food hygiene, including the water used to wash food utensils. This study aimed to determine the number of microbes and identify Escherichia coli and Salmonella sp. on the water that was used to wash tableware by street vendors around UIN Sunan Ampel Surabaya. Water samples before and after being used to wash tableware were obtained from 4 street vendors. The number of microbes in the sample was tested by the Total Plate Count (TPC) and Most Probable Number (MPN) methods, Escherichia coli was identified using Eosin Methylene Blue Agar (EMB) media, and Salmonella sp. was identified using Salmonella-Shigella agar (SSA) and Triple Sugar Iron agar (TSIA) media. Based on the results of TPC test, the water sample before being used to wash tableware had the lowest and highest values that were 3.0 x 102 Cfu/ml and 9.8 x 105 Cfu/ml, respectively, while the water sample after being used to wash tableware had the lowest and highest values that were 4.0 x 102 Cfu/ml and 3.5 x 105 Cfu/ml, respectively. In the MPN test, the water sample before being used to wash tableware had the lowest and highest values that were 15/100ml and 26 100/ml, respectively, while the water sample after being used to wash tableware had the lowest and highest values that were 72/100 ml and 271/ 100 ml, respectively. Escherichia coli was detected in all samples 1&3 while in sample 2 Escherichia coli was detected in the water before it was detected the genus Enterobacter and in samples 4 the genus Enterobacter was detected and all samples water used to wash tableware of street vendors was positive for Salmonella.

EFFECT OF VARIOUS SUBSTRATES IN NON-CIRCULATING HYDROPONIC SYSTEMS AND SOIL MEDIA ON THE GROWTH OF Gynura procumbens Hanik Faizah; Ikhlasotul Fawaidah; Najmatul Millah; Ninik Fadhillah; Muh. Ma’arif
JBIO: jurnal biosains (the journal of biosciences) Vol 6, No 3 (2020): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v6i3.20101

The limitations of conventional soil cultivation in producing medicinal plants emerge an interest in searching various alternative cultivation systems, one of which is the hydroponic system. This study aimed to investigate the effect of various substrates in non-circulating hydroponic systems and soil media on the growth of Gynura procumbens. Non-circulating hydroponic cultivation of Gynura procumbens was carried out using stem cuttings. The stem cuttings were transferred to each net pot containing substrates such as rockwool, tree ferns fiber, and sawdust. The net pots were then placed in a tray comprising AB mix 6 ml/L of water. The lower 2-cm-portion of the net pots was immersed in a nutrient solution. Whereas in the conventional soil cultivation, the stem cuttings were transferred to the polybags containing soil media: compost (1: 1). After 28 days of cultivation, the plant growths were measured. The results showed that the use of various substrates in non-circulating hydroponics increased plant growth compared to conventional soil cultivation. Rockwool treatment obtained the highest number of leaves (14,833 ± 5,269 strands), number of roots (20,333 ± 6,121 stands), fresh weight (1.34 ± 0.511 g) and dry weight (0.088 ± 0.031 g).

Kajian Kelayakan Sumber Air Tanah UIN Sunan Ampel Surabaya dalam Rangka Menuju Eco Campus Ninik Fadhillah; Muh. Ma’arif; Hanik Faizah; Latifatoel Chilmi
Al-Ard: Jurnal Teknik Lingkungan Vol. 5 No. 1 (2019): September
Publisher : Department of Environmental engineering, Faculty of Science and Technology, Islamic State University Sunan Ampel Surabaya

UIN Sunan Ampel requirements of water are fulfilled from groundwater sources. The groundwater sources of UIN Sunan Ampel are from wells where the water is taken with a water pump. The main problem related to the use of groundwater as a fulfillment of water needs is the decline of its quality. The purpose of this study is to analyze the quality of groundwater at UIN Sunan Ampel, to know the quality of groundwater in UIN Sunan Ampel reviewed by Minister of Health Regulation No. RI. 32 of 2017, Formulating the right steps in improving the quality of ground water at Sunan Ampel UIN towards ECO CAMPUS. This research is a descriptive study, in this study the quality of groundwater sources in Sunan Ampel Surabaya UIN will be described base on 3 parameters of water quality (physics, chemistry and biology). The results indicate differences in groundwater quality at UIN Sunan Ampel in terms of physical, chemical and biological parameters. The quality of groundwater sources in UIN Sunan Ampel Surabaya 95% fulfills the standard of environmental health standards and water health requirements for government sanitation hygiene requirements based on physics parameters, but it do not fulfilled the quality standards based on chemical parameters (in the form of pH) and also biology parameters. Improving the quality of ground water at UIN Sunan Ampel Surabaya is needed by improving the management system of sewers and latrines as well as standard construction of wells.

Optimasi Sterilisasi Eksplan Daun Tanaman Lidah Mertua (Sansevieria sp.) pada Kultur In Vitro Ade Tiyan Handayani; Edhi Sandra; Hanik Faizah
Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 1 (2022): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v10i1.4808

The existence of Sansevieria sp. as an ornamental plant that has many benefits preserved. However, conventional propagation requires a lot of materials and time long. Leaf cuttings are only able to produce 1-2 plants in 2 months, and stop when they are aged 5 months. While the separation of tillers (Tillering) only produces 2-3 plants from 1 clump for 5 months. Tissue culture can be the solution, but sterilization must be done to minimize contamination without killing explants. The research aims to find out optimal concentration of HgCl2 and duration of shaking on the sterilization of Sansevieria leaf explants sp.. The research design used a completely randomized design with 2 factors. first factor namely the concentration of HgCl2 with 3 levels (4%, 7%, and 10%,) and the second factor is duration shuffled with 4 levels (3, 5, 7, and 9 minutes). Each treatment includes a negative control performed at the time of explant sterilization in Laminar Air Flow. Data analyzed by test ANOVA or Kruskal-Wallis. Followed by the 5% BNJ test if it is significantly different. Results show that the concentration of HgCl2 has a significant effect on the parameters of the initial time of contamination, the mass sterility of explants, and percentage of browning explants, but had no significant effect on percentage of contaminated explants. Concentration that is too high can slow down the appearance of contamination, prolonging the sterile period, but causing browning of explants. Known that the duration of explant shaking in HgCl2 had no significant effect on all parameter. The use of 10% HgCl2 with a shaking duration of 7 minutes (H3P3) is known to be the most effective optimal for sterilization of explants Sansevieria sp. because it is able to reduce external contamination, prolongs the sterile period up to 12.33 HST, and does not cause too many explants browning that is 33.33%.

Analisis Total Bakteri Coliform dan Identifikasi Escherichia coli pada Makanan dan Minuman di Kantin X Irul Hidayati; Reni Ida Wati; Hanik Faizah
Al-Ard: Jurnal Teknik Lingkungan Vol. 8 No. 1 (2022): September
Publisher : Department of Environmental engineering, Faculty of Science and Technology, Islamic State University Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/alard.v8i1.1488

Food is one of the basic necessities of humans. It is important to pay attention to the quality, safety, hygiene and sanitation of food. Healthy food should contain nutrients, vitamins, and the other essential substances for our body need. The food should be safe for consumption, and free from contamination. Food contamination generally occurs due to coliform bacteria contamination that can cause foodborne disease. This study aimed to analyze the total coliform bacteria and identify Escherichia coli bacteria in food and beverage samples in the canteen X. The design of this study was descriptive research. Samples were obtained from four sellers in canteen X. From each seller, one sample of mixed rice and one sample of iced tea were obtained. Samples were analyzed using the MPN (Most Probable Number) method to determine the total coliform bacteria and EMB (Eosin Methylene Blue Agar) media to identify E. coli. The results showed that all food and beverage samples were tested positive for coliform and E. coli with MPN values exceeding the threshold value. The lowest contamination in the food sample was found in the MA1 and MA2 (460 MPN/gram) and the highest contamination was found in the MA3 and MA4 (>1100 MPN/gram), while the lowest contamination in the beverage sample was found in the MI4 (1100 colonies/100 mL), and the highest contamination was found in the MI1, MI2, and MI3 (>2400 colonies/100 mL).

Physical, Chemical, and Microbiological Quality Analysis of Home Ice Cubes, Beams, and Crystals in Gang Lebar, Wonocolo, Surabaya Anisa Shofiyana Azhari; Esti Tyastirin; Hanik Faizah
Jurnal Biologi Tropis Vol. 22 No. 4 (2022): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i4.4093

Ice cubes are complementary products made from frozen water. If ice cubes have been contaminated by chemical compounds or microorganisms, it will cause various health problems. The purpose of this study was to determine the quality of ice cube, block and crystal houses in the Gang Lebar area, Wonocolo, Surabaya. The physical parameters of ice cubes observed were color, smell and taste. The chemical parameters analyzed were Fe and pH. Microbiological parameters were tested by Total Plate Count (TPC), Most Probable Number (MPN), and Salmonella contamination test. The results showed that by observing the physical parameters of the color of the ice cubes, 1 sample of ice cubes was obtained which met the quality requirements of SNI 3839: 2019, namely sample K1, on the physical observation of smell and taste, 1 sample was obtained which did not meet the requirements, namely the ice block sample. Analysis of pH and Fe chemical parameters showed that all samples met the quality requirements of SNI 3839: 2019 in the pH test. Meanwhile, the Fe test of all samples was declared ineligible. Microbiological analysis of ice cubes showed that 1 sample did not meet the requirements. Furthermore, the results of the MPN coliform test stated that 1 sample met the quality requirements with a value of 7 APM/100mL in sample K1. The Salmonella contamination test found 1 positive sample containing Salmonella, namely in the ice block sample.

Identifikasi Bakteri Toleran terhadap Logam Berat Pb yang Diisolasi dari Air dan Sedimen di Sungai Porong, Sidoarjo Nur Rokhmatul Lailiya; Misbakhul Munir; Esti Tyastirin; Eko Teguh Pribadi; Hanik Faizah
Biotropic : The Journal of Tropical Biology Vol. 6 No. 2 (2022): Biotropic, Volume 6 Nomor 2, 2022
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Porong River is an active river located in the Sidoarjo region, East Java which has been polluted by the Lapindo mudflow containing various chemical compounds, one of which is lead (Pb) heavy metal. Isolation of bacteria in the environment contaminated with Pb allows bacteria that have the potential as bioremediation agents to grow. The purpose of this study was to determine the content of heavy metal Pb and isolate and identify lead (Pb) tolerant bacteria in water and sediment at Porong river, Sidoarjo. Sampling of water and sediment was carried out at 2 sampling points based on the presence of Lapindo mud waste flow. Analysis of the heavy metal content of Pb was carried out using the Atomic Absorption Spectrophotometer (AAS). Bacterial isolation was carried out on NA media containing 10 ppm Pb of heavy metal and incubated for 24 hours at 300C. Bacterial isolates were identified based on the macroscopic and microscopic properties and biochemical tests. The results of the heavy metal content of Pb at sampling point 1 in water was 0.81 ppm, and in sediment was 0.98 ppm while at sampling point 2 in water was 2.93 ppm, and in sediment was 3.88 ppm. The results of bacterial identification obtained as many as 6 bacterial isolates belonging to 3 genera of bacteria including Bacillus, Acinetobacter and Pseudomonas.

Pengaruh Pemberian Infusa Albedo Semangka terhadap Perubahan Ukuran Diameter Pulau Langerhans Pankreas Tikus Jantan (Rattus norvegicus) Diabetes Mellitus Widi Pangestu Wilujeng; Nurul Mahmudati; Ladiya Fitri Ma’rufah; Ninik Fadhillah; Fatmi Indah Hati; Gebby Agnessya Esa Oktavia; Aurira Thrisna Dwi Aprianti; Berlian Rustantina; Nazil Dwi Rahayuningtyas; Azizaturrohmah; Farah Annisa Nurbani; Hanik Faizah; Alfiah Hayati
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1715

Diabetes Mellitus condition (DM) correlates with the damage that occur in pancreas especially in Langerhans islets, one of the damage indicators is diameter changes of Langerhans islets that getting smaller compare with the normal sizes. The use of natural ingredients to control diabetes mellitus can be done by infusing sweet beauty watermelon albedo which can be found abundant in traditional market. Albedo layer of watermelon contain antioxidant compounds that can help to regenerate Langerhans islets of pancreas. This study purpose was to know the effect of giving watermelon’s infusion albedo to diameter of Langerhans islets in diabetes mellitus rats (Rattus norvegicus), and analyze the potential process occurred based on the result as information source for other research. This study used 25 male rats (Rattus norvegicus) as animal model with 5 groups treatment which were normal group, positive group as control (DM without treatment), A group (DM+1000 mg/kgBB watermelon’s infusions albedo), B group (DM+1500 mg/kgBB watermelon’s infusions albedo), and C group (DM+2000 mg/kgBB watermelon’s infusions albedo). After all the treatments, the rats were anesthetized and dissected and then their pancreas was taken. The pancreas was processed into histological slides by fixing the pancreas in a fixative solution (10% formalin) then dehydrated in a series of graded ethanol and embedded in paraffin wax, cut at a thickness of 4 µm, and finally stained with hematoxylin-eosin dye for microscopic observation, according to the stages Histopathological analysis of the gonads was examined using standard methods. Data was collected by measuring the size of Langerhans islets with microscope Olympus CX33 and connected to the raster image application. Data results from this study indicate that watermelon’s infusion albedo had effect to increase the diameter of Langerhans islets of rat with diabetes mellitus

Pengaruh Konsentrasi Gula Terhadap Karakteristik Fisika, Kimia dan Aktivitas Antioksidan Teh Kombucha Daun Belimbing Wuluh (Avverhoa bilimbi Linn.) Fanny Fajrin Aulia Rosyada; Eva Agustina; Hanik Faizah
Rekayasa Vol 16, No 1: April 2023
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/rekayasa.v16i1.16977

One of the fermented traditional drinks that can have a health effect is kombucha. The materials that can be used in making kombucha is wuluh starfruit leaves. The research was conducted to determine the effect of sugar concentration of the physical, chemical characteristic, and antioxidant activities of wuluh starfruit leaf kombucha tea. This research is an experimental study with a complete randomized design method (CRD) consisting of 3 treatments. Kombucha wuluh starfruit leaves are made with a sugar concentration of 10%, 15% and 20%. The physical characteristics tested include organoleptic tests and the chemical characteristics include a test of the pH value, the total levels of titrated acids (tat), phenolics statisticallyÂ analyzedÂ usingÂ the Kruskal Wallis test and the Mann Whitney test. The alcohol data and antioxidant activity (IC50) were analyzed descriptively. The results of the Kruskal Wallis testÂ have a significant difference in the pH. The best result based on the physical, chemical characteristic and antioxidant activity is were kombucha with a sugar concentration of 10% with a pH value of 3, a tat content of 0.11%, a phenolic content of 87.33 mg/ml GAE, an alcohol content of 0.41% and an IC50 value of 3.65 ppm.

Co-Authors Ade Tiyan Handayani AGUSTINA, EVA Alfiah Hayati Anisa Shofiyana Azhari Aurira Thrisna Dwi Aprianti Azizaturrohmah Berlian Rustantina Edhi Sandra Eko Teguh Pribadi Esti Tyastirin Evi Fatimatur Rusydiyah Fahimatul Ula Fanny Fajrin Aulia Rosyada Farah Annisa Nurbani Fatmi Indah Hati Gebby Agnessya Esa Oktavia Hernik Farisia Husniyatus Salamah Zainiyati Ikhlasotul Fawaidah Irul Hidayati Ladiya Fitri Ma’rufah Latifatoel Chilmi Mei Lina Fitri Kumalasari Millah, Najmatul Misbakhul Munir Moch. Irfan Hadi Muh. Ma’arif Muhamad Ratodi Muhammad Yusuf Alamudi Nazil Dwi Rahayuningtyas Ninik Fadhillah Nur Rokhmatul Lailiya Nurul Mahmudati Qurrotul Ayun Reni Ida Wati Risa Purnamasari Sri Hidayati Tatag Bagus Prakarsa Widi Pangestu Wilujeng

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