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PENGARUH KONSENTRASI SUKROSA TERHADAP PERTUMBUHAN KALUS, SENYAWA METABOLIT SEKUNDER DAN AKTIVITAS ANTIOKSIDAN BAWANG MERAH (Allium ascalonicum L.) Nurfadhila, Syilvani Citra; Sari, Yanti Puspita; Astuti, Puji
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 16 No 2 (2024): BIOPROSPEK: Jurnal Ilmiah Biologi
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/bp.v16i2.1346

Bawang merah (Allium ascalonicum L.) merupakan komoditas sayuran yang memiliki banyak manfaat dalam bidang pangan dan pengobatan. Untuk memaksimalkan pemanfaatan bawang merah sebagai tanaman obat, digunakan bahan berupa kalus yang diperoleh melalui teknik kultur jaringan. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi sukrosa terhadap pertumbuhan kalus, kandungan senyawa metabolit sekunder, dan aktivitas antioksidan bawang merah. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan perlakuan konsentrasi sukrosa, yaitu 0, 15, 30, 45, dan 60 g/L dalam media Murashige Skoog (MS) yang ditambahkan zat pengatur tumbuh (ZPT) pikloram 5 mg/L + kinetin 3 mg/L. Setiap perlakuan diulang lima kali sehingga total percobaan sebanyak 25 unit. Pengujian meliputi induksi kalus, uji fitokimia (alkaloid, fenolik, flavonoid, saponin, steroid-triterpenoid), dan uji aktivitas antioksidan. Hasil penelitian menunjukkan bahwa penambahan sukrosa 30 g/L memberikan hasil terbaik dalam menginduksi kalus, dengan rata-rata waktu tumbuh kalus 33,6 hari, tekstur kalus remah-kompak, warna kalus kuning, dan berat rata-rata kalus 0,583 ± 0,278 g. Kandungan metabolit sekunder meliputi alkaloid, fenolik, flavonoid, dan saponin. Aktivitas antioksidan mencapai 17,691 ppm, yang termasuk dalam kategori antioksidan sangat kuat.

Root Induction of Borneo Prima Tangerine (Citrus Reticulata) By In Vitro Method Using Natural Plant Growth Regulator Raja Banana (Musa Paradisiaca Var. Raja) and Synthetic Naphthalene Acetic Acid (NAA) Muhammadiyah, Muhammadiyah; Sari, Yanti Puspita; Susanto, Dwi
Al-Kauniyah: Jurnal Biologi Vol 18, No 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.40714

Borneo prima tangerine (Citrus reticulata) is a plant species from East Kalimantan, which is expected to become one of the leading citrus in the country. The obstacle to developing this citrus is that cultivation is still limited, so efforts are needed to cultivate plants using in vitro techniques. The process of plant multiplication has been done, the next step is rooting. The purpose of this research is to know the type and concentration of plant growth regulators (PGR) that are optimal in stimulating root growth in Borneo prima tangerine plants. This study used a completely randomized design consisting of 9 treatments, namely control (without the addition of PGR), the addition of natural PGR raja banana extract with concentrations of 25; 50; 75; and 100 g/L, synthetic PGR Naphthalene Acetic Acid (NAA) concentrations of 0.5; 1.0; 1.5; and 2.0 mg/L, each treatment with 3 replicates. The results showed the addition of raja banana extract 75 g/L produced the fastest root emergence time of 3.33 days, the addition of NAA 0.5 mg/L produced the highest number of roots, namely 4.67 roots, and plantain extract 50 g/L produced the longest roots, namely 4.50 cm. This means that the addition of raja banana extract gives the best results in inducing root formation.

Hubungan Pengetahuan dan Sikap dengan Kesiapsiagaan Menghadapi Risiko Gempa Bumi dan Tsunami pada Siswa SMA Huriani, Emil; Sari, Yanti Puspita; Harningsih, Nurfa Rahim
Dunia Keperawatan: Jurnal Keperawatan dan Kesehatan Vol 9 No 3 (2021): Dunia Keperawatan: Jurnal Keperawatan dan Kesehatan
Publisher : School of Nursing, Faculty of Medicine and Health Science, Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.851 KB)

Earthquake and tsunami have high risk level in Padang State that predict earthquake 8.9 Richter of MentawaiMegatrust. Padang State Senior High School 2 located in Red Zone of earthquake and tsunami and also close withBanda Bakali River. There are still found some students whose have not expressed enough knowledge related toearthquake and tsunami preparedness and not ready if disaster occur. This study aims to determine therelationship of knowledge and attitude with preparedness to confront earthquake and tsunami risk on students atPadang State Senior High School 2. Research design used analytics descriptive with cross sectional studyapproach to 280 respondents. The instrument of research consisted of 4 parts of questionnaire, which aredemographics data, knowledge, attitude, and preparedness. Research analysis used a comparative test by usingsoftware computer. The results of this study showed that 55.4% had high knowledge, 53.2% good attitudes, and66.1% moderate preparedness. In this result found that significant relationship between knowledge andpreparedness (p = 0.001) and significant relationship between attitude and preparedness with significance value(p = 0.000). Recommended to school to improve learning which more applicable for health promotion especiallyon earthquake and tsunami disasters that has been integrated in curriculum subject and held disasters simulationsearlier especially for new students

Mapping the conceptual framework of psychological well-being among pregnant adolescents and influential factors: A scoping review Sari, Yanti Puspita; Armi, Yeltra; Lin, Chung-Ying
Pediomaternal Nursing Journal Vol. 11 No. 2 (2025): VOLUME 11 NO 2 SEPTEMBER 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/pmnj.v11i2.66872

Introduction: Pregnancy in adolescents has the potential risk of disrupting psychological well-being. Understanding how psychological well-being is conceptualized and assessed among adolescents, as well as identifying influential factors, is essential for developing appropriate and effective interventions. This review aims to map the conceptual approaches and assessment methods used to evaluate psychological well-being among pregnant adolescents and to identify influential factors associated with psychological well-being during pregnancy. Methods: The study procedures were carried out using a scoping review that followed the Joanna Briggs Institute approach. Literature searches included terms from Emtree, MeSH, and CINAHL headings across 5 databases, including Embase, Medline (Ovid), CINAHL, Cochrane, and ScienceDirect until April 2024. After the search, a total of 8 studies were included, which met the needed criteria. Results: This review identified 5 conceptual approaches used to understand and assess psychological well-being in pregnant adolescents, namely (i) self-esteem, (ii) measures on anxiety, depression, emotional bonding, general positive affect, and the absence of psychological distress, (iii) discrimination experiences, self-perceptions, and social barriers experienced, (iv) positive and negative mood concepts, and (v) positive psychological functioning. The results showed that several factors were associated with psychological well-being of pregnant adolescents, including educational level, neighborhood support, financial constraints, rejection from close people, trust, and attachment with caregiver. Conclusion: This review shows the need for a more comprehensive and multidimensional approach to conceptualizing and assessing psychological well-being in pregnant adolescents. The complexity of this issue demands a thorough and inclusive approach that focuses on individual and social factors. Keywords: adolescent pregnancy; psychological well-being; adolescent growth and development

Root Induction of Borneo Prima Tangerine (Citrus Reticulata) By In Vitro Method Using Natural Plant Growth Regulator Raja Banana (Musa Paradisiaca Var. Raja) and Synthetic Naphthalene Acetic Acid (NAA) Muhammadiyah, Muhammadiyah; Sari, Yanti Puspita; Susanto, Dwi
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.40714

Borneo prima tangerine (Citrus reticulata) is a plant species from East Kalimantan, which is expected to become one of the leading citrus in the country. The obstacle to developing this citrus is that cultivation is still limited, so efforts are needed to cultivate plants using in vitro techniques. The process of plant multiplication has been done, the next step is rooting. The purpose of this research is to know the type and concentration of plant growth regulators (PGR) that are optimal in stimulating root growth in Borneo prima tangerine plants. This study used a completely randomized design consisting of 9 treatments, namely control (without the addition of PGR), the addition of natural PGR raja banana extract with concentrations of 25; 50; 75; and 100 g/L, synthetic PGR Naphthalene Acetic Acid (NAA) concentrations of 0.5; 1.0; 1.5; and 2.0 mg/L, each treatment with 3 replicates. The results showed the addition of raja banana extract 75 g/L produced the fastest root emergence time of 3.33 days, the addition of NAA 0.5 mg/L produced the highest number of roots, namely 4.67 roots, and plantain extract 50 g/L produced the longest roots, namely 4.50 cm. This means that the addition of raja banana extract gives the best results in inducing root formation.

Pembekalan Teknik Propagasi Kultur Jaringan Tumbuhan bagi Guru SMA/Sederajat di Samarinda untuk Mendukung Kurikulum Merdeka Susanto, Dwi; Yuliatin, Ervinda; Samsurianto, Samsurianto; Sari, Yanti Puspita; Manurung, Hetty
Jurnal SOLMA Vol. 12 No. 3 (2023)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v12i3.12944

Background: Transisi kurikulum 2013 menjadi kurikulum Merdeka menuntut guru untuk lebih kreatif, aplikatif, dan mampu memacu daya kritis siswa dalam memahami mata Pelajaran pilihan. Namun, mata pelajaran pilihan siswa di bidang Bioteknologi Modern seperti Kultur Jaringan Tumbuhan belum dipahami dengan baik secara praktik. Kegiatan ini bertujuan meningkatkan keterampilan praktikum teknik propagasi tumbuhan melalui pembekalan teknik kultur jaringan tumbuhan bagi guru SMA/sederajat bidang Biologi di Samarinda dan sekitarnya. Metode: Pengabdian ini dilakukan oleh dosen Biologi FMIPA Universitas Mulawarman, dan diikuti oleh 22 guru Biologi dan pertanian di Samarinda dan sekitarnya. Kegiatan pengabdian terdiri dari 4 tahapan yaitu tahap awal, tahap pembekalan teori, tahap praktikum, dan tahap evaluasi. Hasil: Adanya peningkatan pemahaman peserta di bidang kultur jaringan tumbuhan dari level ‘kurang’ menjadi ‘baik’. Evaluasi pemaparan materi dan kesesuaian teori dan praktek mencapai kategori sangat baik dengan presentase masing-masing 80% dan 92%. Refleksi pembekalan menunjukkan 80% peserta berpendapat kegiatan ini menambah wawasan dan menyenangkan. Kesimpulan: Kegiatan terlaksana sesuai target dan dapat berkontribusi dalam proses pembelajaran siswa di sekolah terutama di bidang kultur jaringan tumbuhan.

Lived experiences of domestic violence among Minangkabau women: A phenomenological study Sari, Yanti Puspita; Abdullah, Khatijah Lim; Hermalinda, Hermalinda
Jurnal Keperawatan Padjadjaran Vol. 13 No. 3 (2025): Jurnal Keperawatan Padjadjaran
Publisher : Faculty of Nursing Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkp.v13i3.2637

Objective: This study aimed to explore the lived experiences of women who have faced domestic violence, focusing on the causes of the violence, its impact on their health, and the decision-making processes they undertook in response. Method: This study used a phenomenological study approach. The inclusion criteria were women from the Minangkabau tribe, aged 18-40, married or previously married, and with children. Participant recruitment was conducted through the Pusat Pelayanan Terpadu Pemberdayaan Perempuan dan Anak/ Integrated Service Center for the Empowerment of Women and Children) and the Women’s Crisis Center (WCC) “Nurani Perempuan”, based on predetermined inclusion criteria. Data collection continued until thematic saturation was reached. Nine women were involved. In-depth interviews and field notes were transcribed verbatim, and theme analysis was performed using the seven-step Collaizzi technique. Results: Four themes emerged from the data: (1) failure to resolve family conflict; (2) physical and psychological despair; (3) inability to make decisions; (4) need for external support. Participants’ experiences reflect the erosion of traditional protective systems due to cultural shifts, economic dependence, and societal pressures. Conclusions: The study highlights that unresolved family conflict, poor health outcomes, and limited decision-making power shape women's vulnerability to domestic violence. Strengthening community-based support and culturally sensitive interventions is recommended to empower women and reduce the risk of domestic violence.

Pembekalan Teknik Ekstraksi, Skrining, dan Uji Antimikrobia Bahan Alam bagi Guru-Guru SMA Di Kota Samarinda Kusumawati, Eko; Nugroho, Rudy Agung; Aryani, Retno; Manurung, Hetty; Sari, Yanti Puspita
Jurnal Pemberdayaan Masyarakat Vol 6 No 1 (2021): Mei
Publisher : Direktorat Penelitian dan Pengabdian kepada Masyarakat (DPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21067/jpm.v6i1.4040

Sebagai salah satu faktor penting dalam pendidikan, guru harus meningkatkan pengetahuan, baik teoritis maupun keterampilan, seperti praktik laboratorium, terutama untuk guru biologi. Berdasarkan hal ini, tujuan program pengabdian masyarakat adalah untuk meningkatkan keterampilan teoritis dan praktis guru biologi SMA. Program pengabdian masyarakat ini diikuti oleh 30 guru dari berbagai SMA di Samarinda. Teori tentang ekstraksi, penyaringan, dan teknik uji antimikrobia dari sumber daya alam dilakukan di ruang kelas, sedangkan penggunaan rotary evaporator, freeze drying, skrining uji fitokimia, dan uji antimikrobia dilakukan di laboratorium yang dibantu oleh teknisi laboratorium. Setelah menerima program pengabdian masyarakat ini, nilai rata-rata kepuasan yang diberikan oleh para guru melalui kuisioner yaitu: (skala 5) sebanyak 49%, baik (skala 4) sebanyak 47%, cukup (nilai skala 3) ) sebesar 5% dan lebih kecil (skala nilai 2) sebesar 1%. Kesimpulan, kegiatan pengabdian ini berjalan dengan baik dan mempunyai manfaat baik bagi guru biologi SMA di Samarinda.

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