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All Journal Natural Science: Journal of Science and Technology EUGENIA Jurnal Natur Indonesia Prosiding Seminar Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Biotechnology Journal of Tropical Biodiversity and Biotechnology Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)

LANGKAH SEMBIRING

Laboratorium Mikrobiologi Fakultas Biologi Universitas Gadjah Mada

Author-ID : 375510

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Environmental Science Immunology & microbiology Materials Science & Nanotechnology

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Kondisi Optimum untuk Produksi Kitinase dari Streptomyces Rkt5 dan Karakterisasi pH dan Suhu Enzim Yurnaliza, Yurnaliza; Margino, Sebastian; Sembiring, Langkah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Chitinase is chitin degrading enzyme which is produced by Streptomyces Rkt 5 is isolated microorganism from peanut rhizosfer. This enzyme and its microorganism can be used in many agricultural, medicine and industrial purposes. The aim of the research was to find out the optimum condition for production of chitinase and to characterize of pH and temperature to chitinase activity. Optimalizing production the research had 4 treatments. The optimum conditions were achieved at mineral liquid medium containing with chitin 0,2% (w/v) as inducer, 10% (v/v) inoculum, pH 7 and 48 hours incubation. The crude enzyme was partially purified by salting out with 70% ammonium sulfate resulted in 3.31 time more purity enzyme than the crude one. This enzyme had maximum activity at 50oC and pH 5.5.

Analisis Keanekaragaman Isolat Bacillus thuringiensis yang Patogenik terhadap Serangga Hama Kubis (Crocidolomia binotalis) dengan Pendekatan Sistematika Numerik Salaki, Christina L.; Situmorang, Jesmandt; Sembiring, Langkah; Handayani, Niken
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Diversity of B. thuringiensis (Bt.) isolates pathogenic to C. binotalis was determined by using Numerical Systematic Method. Ten isolates were taken to represent 34 pathogenic isolates along with two reference strains namely B. thuringiensis serovar kurstaki and B. thuringiensis serovar israelensis. The test isolates were examined for 89 phenotypic characters by using convensional method for colonial and cell morphology (37 characters) as well as physiological characteristics (3 characters) but biochemical characterization (49 characters) was conducted by using commercial API-50 CHB procedures. All phenotypic characters existed in one of two mutually exclusive states and were either scored plus (1) of minus (0). The binary data were prepared in Programmerâ€™s File Editor (PFE) software. The data then were analysed by using the Multi Variate statistical Package (MVSP) Plus-Version 3.1 using the Simple Matching Coefficient (SSM). Clustering was achieved using the UPGMA algorithm. The results were presented as dendrograms. It was obtained that the test isolates were clearly assigned to two distinct multimembered clusters defined by 79.6 similarity level (S-level) in the SSM, UPGMA analysis. The two distinct clusters represented by each of two widely known different group of Bt. strains, namely serovar israelensis and serovar kurstaki. The first cluster contained reference strain of B. thuringiensis serovar israelensis, and two of the isolates (Slk2.3, and YPPA1) and the second cluster contained another reference strain of B. thuringiensis serovar kurstaki, and 8 of the isolates. Therefore, it strongly suggested that the application of numerical-fenetic analysis could provide a tool to unravel the strain diversity belong to B. thuringiensis.

ISOLASI DAN IDENTIFIKASI STREPTOMYCETES DARI RIZOSFER JAGUNG (ZEA MAYS L.) YANG BERPOTENSI SEBAGAI PENGHASIL ANTIBIOTIKA Ambarwati, Ambarwati; Soegihardjo, C. J.; Sembiring, Langkah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

In attempt to understand the diversity of Actinomycetes that is potential to be antibiotic producer, Streptomycetes were isolated and identified from soil sample taken from rhizosphere and non-rhizosphere of corn (Zea mays L.). The best antibiotic producers were identified by Scanning Electron Microscopy analysis and the identification of antibiotic produced conducted by using Thin Layer Chromatography analysis. The result of the study showed that 58 isolates were assigned to 17 colour groups. Ten isolates among the representatives of 17 colour groups were found potential to be antibiotic producer. Four isolates out of 10 isolates could inhibit both Staphylococcus aureus ATCC 25923 and Bacilus subtilis FNCC 0060, one isolate could inhibit only Staphylococcus aureus ATCC 25923 and five isolates could inhibit only Bacilus subtilis FNCC 0060. But no isolate could inhibit Escherichia coli ATCC 35218 and Salmonella typhimurium FNCC 0164. Among 10 isolates of antibiotic producer it was found that only one isolate (RNJ14) could strongly inhibit Staphylococcus aureus ATCC 25923 with inhibition zone diameter of 32.33 mm. On the bases of Thin Layer Chromatography analysis, the antibiotic produced by the isolate RNJ14 was identified to be lincomycin. Therefore it could be concluded that streptomycetes isolated from the rhizosphere and non-rhizosphere of corn (Zea mays L.) were potential to produce antibiotic.

Expression, Characterisation and Structural Homology Modelling of Recombinant Mercuric Reductase of Streptomyces sp. AS2 Khasanah, Anis Uswatun; Putri, Wahyu Aristyaning; Rahayu, Hanum Mukti; Sembiring, Langkah; Purwestri, Yekti Asih
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 4 (2024): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.88773

Mercury pollution poses a significant environmental challenge worldwide, prompting extensive efforts over the past two decades to combat its detrimental effects. Cloning merA from Streptomyces sp. AS2 (Accession numbers LC026157) into the expression vector pET-28c (+) marks a critical advancement in this field, necessitating further investigation into the expression and structural analysis of the resulting recombinant mercuric reductase protein. This study aimed to optimise the expression and characterise the structural MerA protein. The study involved the expression of merA from AS2 isolate in the host Escherichia coli BL21 and the measurement of mercuric reductase using SDS-PAGE. Induction of E. coli BL21 was optimized by adding IPTG concentration and incubation time. Purification of mercuric reductase was attempted using ammonium sulphate precipitation, dialysis, and column chromatography. Protein structural characterisation was conducted using computational modelling tools Swiss-Model and Phyre2. Expression of merA from AS2 isolate was successfully performed in E. coli BL21, with SDS-PAGE showing a dominant band in the 55-70 kDa range using IPTG concentration 1 and 1,2 mM and 18-hour incubation time. The specific activity of mercuric reductase was obtained at an enzyme concentration of 294.07 Unit/mg. Protein structural characterisation revealed homology with Lysinibacillus sphaericus (Swiss-Model) and similar folding to c5c1Yc, a known mercuric reductase from the same species using Phyre2. The successful expression of recombinant pET-28c (+)-MerA in E. coli BL21 offers new opportunities for bioremediation efforts targeting mercury contamination.

Isolasi dan Uji Aktivitas Antibakteri Actinomycetes dari Rhizosfer Bakau di Hutan Bakau Torosiaje Gorontalo Retnowati, Yuliana; Sembiring, Langkah; Moeljopawiro, Sukarti; Djohan, Tjut S.; Soetarto, Endang S.
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2017: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.963 KB)

Actinomycetes penghasil antibiotik telah dieksplorasi dari berbagai sumber di lingkungan, terutama lingkungan ekstrim.Hutan bakau Torosiaje di Provinsi Gorontalo memiliki kondisi geomorfologi yang unik berupa ekosistem hutan bakau karsdengan dua tipe area yaitu tipe fringe dan overwash mangrove yang tersusun oleh jenis bakau yang bervariasi. Penelitian inidi desain untuk mendapatkan isolat Actinomycetes dari rhizosfer berbagai jenis bakau di hutan bakau Torosiaje Gorontalodan menganalisis aktifitas antibakteri melawan bakteri patogen. Sampel tanah dikoleksi dari rizosfer tujuh jenis pohon bakauyaitu Rhizophora mucronata dan Bruguiera gymnorhiza pada tipe hutan overwash, Rhizophora apiculata, Bruguieragymnorhiza pada zona middle tipe hutan Fringe, Avicenia marina, Xylocarpus sp, Ceriops tagal dan Soneratia alba padazona upper tipe hutan fringe. Pre-treatmen sampel tanah berdasarkan metode panas basah pada suhu 60oC selama 15 menit.Isolasi selektif Actinomycetes menggunakan medium Starch Casein Agar yang disuplementasi dengan cyclohexamide dannystatin. Seleksi isolat penghasil antibiotik berdasarkan metode agar blok menggunakan bakteri uji Eschericia coli,Staphylococcus aureus dan Bacillus subtillis. Aktifitas antibakteri ditandai dengan pembentukan zona hambat disekitarpertumbuhan actinomyctes. Diameter zona hambat dan diameter koloni Actinomycetes diukur untuk menentukan indekszona hambat. Hasil penelitian diperoleh sebanyak 167 isolat Actinomycetes yang terdistribusi pada rizosfer 7 jenis bakau. 77isolat Actinomycetes menunjukkan aktifitas antibakteri melawan bakteri patogen, terdiri dari 52 isolat melawan bakteriGram-positif (narraw spectrum) dan 25 isolat melawan bakteri Gram-positif dan Gram-negatif (broad spectrum). IsolatActinomycetes penghasil antibiotik memiliki karakter morfologi yang bervariasi yang didominasi oleh koloni berwana putihdan pigmen terdifusi berwarna kekuningan sampai coklat dan dikelompokkan kedalam 15 grup.

Co-Authors Ambarwati Artama, I Wayan Tunas Bambang Setiaji C. J. Soegihardjo Charlie Ester de Fretes Christina Salaki Dwi Suhartanti Elisa Nurnawati ENDANG S. SOETARTO Endang Sutriswati Rahayu Helen Joan Lawalata Jamhari Jamhari Jesmandt Situmorang Jusup Subagja, Jusup Khasanah, Novita Uswatun Laksono Trisnantoro Lela Susilawati Lisa Lisdiana Masashi Kawaichi, Masashi Niken Handayani Nurhayani H. Muhiddin, Nurhayani H. Orryani Lambui Putri, Wahyu Aristyaning Rahayu, Hanum Mukti S. Rahayu, S. Sebastian Margino Selvi Marcellia Sri Darmawati Subagja, Yusup Subagus Wahyuono Sukarti Moeljopawiro Syaiful Anwar Tanti Azizah S Tjut S. Djohan Tri Ardyati Wayan T. Artama Wayan T. Artama Widayati, Wiwik E. Widya Asmara Yekti Asih Purwestri Yuliana Retnowati Yurnaliza, Yurnaliza

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