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Enny Tantini Setiatin
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Identification of Gene Diversity of Melanocortin 4 Receptor for Commercial Rabbit Breeds Kamila, Firda Tasya; Kamalludin, Mamat Hamidi; Sutopo, Sutopo; Lestari, Dela Ayu; Setiatin, Enny Tantini; Agustine, Ananda Dwi; Latifa, Alfa Putri; Nabilah, Zata; Philco, Syaddad Verahry; Setiaji, Asep
Buletin Peternakan Vol 49, No 1 (2025): BULETIN PETERNAKAN VOL. 49 (1) FEBRUARY 2025
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v49i1.99303

Abstract

Molecular genetic markers are regularly implemented in rabbit management for better breeding by selecting for specific genotypes. This study aimed to examine the diversity of the melanocortin-4 receptor gene in rabbits in Central Java, Indonesia. A total of 35 bucks were used in this study, comprising 10 New Zealand White, 11 Hyla and 14 Hycole. The DNA was extracted by Gene JET Whole Blood Genomic DNA extraction kit. Single Nucleotide Polymorphism (SNP) was detected by Polymerase Chain Reaction (PCR) and DNA sequencing. Hot carcass weight, cold carcass weight, reference weight and cut point 1-5 weight were among the parameters that were measured. This study showed that Melanocortin 4 Receptor (MC4R) genes were polymorphic, containing missense mutations and one SNP at 519G→A SNP 519GA had two alleles, A and G, with three genotypes (AA, GG, and AG). The rabbit population did not achieve HardyWeinberg Equilibrium (HWE) and showed a low level of genetic diversity as indicated by the heterozygosity results. The result of this study concluded that the frecuency AG as a genotype was higher than either GG or AA and A had a higher allele frequency than G. We recommended improving the validity of the data across various rabbit populations
Performa Pertumbuhan Tiga Varietas Ayam Kedu Umur 0–10 Minggu Akramullah, Muh; Kurnianto, Edy; Lestari, Dela Ayu; Setiatin, Enny Tantini; Setiaji, Asep
Jurnal Sain Peternakan Indonesia Vol 20 No 3 (2025)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.20.3.123-127

Abstract

This study aimed to evaluate the growth performance of Red-Combed Kedu (RCK), Black-Combed Kedu (BCK), and White Kedu (WK) chickens based on sex during the early growth phase (0 to 10 weeks of age). A total of 136 Kedu chickens were raised under uniform management conditions, consisting of 99 RCK (65 roosters and 34 hens), 29 BCK (5 roosters and 24 hens), and 8 WK (6 roosters and two hens). Body weight was recorded weekly and analysed using a two-way General Linear Model (GLM) in SAS to assess the effects of chicken variety, sex, and their interaction. The Shapiro–Wilk test confirmed that the data were normally distributed (p > 0.05). The results showed that sex had a significant effect on body weight from weeks 3 to 10 (p < 0.05), with roosters consistently exhibiting higher weights than hens. Significant differences among chicken varieties were observed only in the hen group at weeks 9 and 10, where BCK hens had higher body weights (p < 0.05) than WK hens, while RCK hens showed intermediate values. In contrast, no significant differences were found among rooster groups at any age. These findings suggest that genetic factors play an essential role in influencing growth performance in Kedu chickens, particularly in hens. This information provides a valuable foundation for breeding selection and the conservation of local chicken genetic resources.
Mastitis sebagai Indikator Kesejahteraan Sapi Perah yang Dipelihara secara Zero Grazing di Daerah Tropis Harjanti, Dian Wahyu; Solehah, Dina Amalia; Setiatin, Enny Tantini
Jurnal Agripet Vol 23, No 2 (2023): Volume 23, No. 2, Oktober 2023
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v23i2.25551

Abstract

ABSTRACT. Penelitian ini bertujuan untuk mengkaji prevalensi mastitis sebagai indikator kesejahteraan sapi perah yang dipelihara secara zero grazing di daerah tropis. Penelitian dilakukan secara observasional dengan metode survei dan pengambilan sampel secara purposive sampling. Materi yang digunakan dalam penelitian ini yaitu 45 ekor sapi perah laktasi yang dipelihara secara zero grazing pada kandang koloni dengan paritas I IV. Parameter yang diamati adalah tingkat peradangan ambing yang diuji menggunakan California Mastitis Test (CMT) dan jumlah sel somatis, produksi susu dan skor kebersihan ternak yang terdiri dari kebersihan kaki belakang bawah, kebersihan kaki belakang atas dan kebersihan ambing. Analisis yang digunakan yaitu analisis regresi linear sederhana dan korelasi Spearman. Hasil penelitian menunjukkan terdapat kuartir yang sehat sebesar 75% (135 kuartir) sedangkan 25% terindikasi mastitis. Terdapat hubungan yang nyata (P0,05) antara produksi susu dengan peradangan ambing (CMT dan sel somatis) secara linier negatif dengan persamaan Y = 10,945 2,650 X dan Y = 10,284 2,847E6 X. Tingkat peradangan dan kebersihan kaki belakang atas dan kaki belakang bawah tidak memiliki hubungan yang nyata (P0,05) tetapi memiliki hubungan yang nyata dengan kebersihan ambing (P0,05). Hasil tersebut menunjukkan bahwa pemeliharaan sapi perah secara zero grazing di daerah tropis dengan animal welfare yang baik menghasilkan prevalensi mastitis yang rendah sehingga produksi susu dapat optimal.(Mastitis as an indicator of dairy cow welfare reared in a zero-grazing system in the tropics)ABSTRAK. This study was aimed to examine the prevalence of mastitis as an indicator of the welfare of dairy cows reared on a zero grazing basis in the tropics. The study was conducted by observational with survey method and sampling by purposive sampling. The material used in this study were 45 lactating dairy cows reared in zero grazing in colony pens with parity I IV. The parameter observed was the level of udder inflammation which was tested using the California Mastitis Test (CMT) and somatic cell count, milk production and cleanliness score of livestock consisting of cleanliness lower hind legs, cleanliness upper hind legs and cleanliness udder. The analysis used is simple linear regression analysis and spearmen correlation. The results showed that there were healthy quarters of 75% (135 quarters) while 25% indicated infected with mastitis. There was a significant relationship (P0.05) between milk production and udder inflammation (CMT and somatic cell count) with the equation Y = 10.945 2.650 X and Y = 10.284 2.847E6 X. The level of inflammation and cleanliness of the upper hind legs and lower hind legs did not have a significant relationship (P0.05) but had a significant relationship with udder cleanliness (P0.05). These results indicate that the maintenance of dairy cows with zero grazing in the tropics has a prevalence of mastitis which affects milk production and thus affects the welfare of dairy cows.
Co-Authors Agustine, Ananda Dwi Akramullah, Muh Casmuti, Casmuti Daud Samsudewa Dela Ayu Lestari, Dela Ayu Dian Wahyu Harjanti Dona Bella Apri San, Dona Bella Apri Dwi Wijayanti Edy Kurnianto Fattah, Alvian I Ketut Gorde Yase Mas, I Ketut Gorde Kamalludin, Mamat Hamidi Kamila, Firda Tasya Latifa, Alfa Putri Nabilah, Zata Nelva Lestari Silaban ono syamyono Ono Syamyono Philco, Syaddad Verahry Rachmat Heru Sulaksono Setiaji, Asep Solehah, Dina Amalia Sutiyono . sutiyono sutiyono Sutopo Sutopo Wibowo, Leonardus Abieza Yon Soepri Ondho Zulkarnain .