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All Journal HAYATI Journal of Biosciences Microbiology Indonesia Medical Journal of Indonesia Journal of Biomedicine and Translational Research Narra J Proceedings of the International Conference on Nursing and Health Sciences

Amin Soebandrio

Departemen Mikrobiologi Klinik Fakultas Kedokteran, Universitas Indonesia

Author-ID : 383481

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health

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Amantadine Resistant of Indonesian H5N1 Subtype Influenza Viruses During 2003-2008 NI LUH PUTU INDI DHARMAYANTI; FERA IBRAHIM; AMIN SOEBANDRIO
Microbiology Indonesia Vol. 4 No. 1 (2010): April 2010
Publisher : Indonesian Society for microbiology

The M2 protein of 146 avian influenza (AI) viruses data available in public database (NCBI), including 20 AI isolates used in this study, were sequenced at the M2 protein to find out the probability of mutation and the increase of resistance to amantadine after more than 5 years of their circulation in Indonesia. The results showed that during 2003-2008, around 62.58% (92/147) AI viruses in Indonesia have showed resistance to amantadine and 10 of them have dual mutations at V27A and S31N.

The Genetic Drift of Indonesian Avian Influenza A H5N1 Viruses During 2003-2008 NI LUH PUTU INDI DHARMAYANTI; GINA SAMAAN; FERA IBRAHIM; RISA INDRIANI; . DARMINTO; AMIN SOEBANDRIO
Microbiology Indonesia Vol. 5 No. 2 (2011): June 2011
Publisher : Indonesian Society for microbiology

The avian influenza A H5N1 outbreaks started in 2003 and Indonesia introduced a vaccination campaign in 2004 to control the disease. In 2007, anecdotal reports about reduced vaccine effectiveness were received from commercial farmers. This paper describes the evolution of viruses in Indonesia up till 2008 and focus on viruses from vaccinating farms reporting vaccine failure were compared to viruses isolated from outbreak areas with no vaccination program. Result of the study revealed that viruses from vaccinated chickens had more extensive mutation at the HA molecule compared to chicken and other avian species without vaccination. Substitutions occurred at the HA gene level as well as at NA, M1 and NS1 genes. Viruses isolated and characterized form 2008 vaccinated flocks had substitutions that were unique and different with the old viruses. The recommendation arising from this study to the avian influenza disease control program in Indonesia is that continuous monitoring of genetic character of viruses and the vaccine seed strain should be updated periodically and matched with the virus circulated in the field.

Growth Characteristics of Chikungunya Virus Isolate from Indonesia in Various Human Cell Lines in vitro Oktaviani Naulita Turnip; OKTAVIANI N. TURNIP; RAHMA F. HAYATI; RIZKA ALAWIYAH; BENEDIKTUS YOHAN; DIONISIUS DENIS; ANOM BOWOLAKSONO; AMIN SOEBANDRIO; R. TEDJO SASMONO
Microbiology Indonesia Vol. 13 No. 1 (2019): March 2019
Publisher : Indonesian Society for microbiology

Chikungunya (CHIK) fever, a febrile illness caused by Chikungunya virus (CHIKV) infection, is one of mosquito-borne viral diseases affecting people living in the tropical and subtropical regions in the world. The pathogenesis of the disease is yet to be completely unraveled, and research on CHIK has been conducted by employing various methods, including using cell lines to investigate the biological characteristics of CHIKV in vitro. To assess the suitability of human cell line model for CHIK study, various human cell lines including A549, Huh7, and HepG2 were infected with CHIKV and assayed for their susceptibility to infection. The MTT and plaque assay methods were performed to measure cell viability and virus growth kinetics, respectively. Fluorescence-activated Cell Sorting (FACS) and immunofluorescence assay were performed to measure the proportion of infected cells in the system and their morphological visualization. Both A549 and Huh7 human cell lines showed stable high cell viability upon infection while CHIKV growth kinetics were significantly lower in these cells compared to Vero-CCL81, a monkey cell line that is routinely used in other arboviruses research. Interestingly, we observed significantly different results in HepG2 human cell line, in which cell viability and CHIKV growth kinetics were significantly higher. FACS and immunofluorescence assay confirm the higher infection rate of CHIKV in HepG2 than A549 human cell line. We concluded herethat human hepatocytes HepG2 cell line was susceptible to Asian Genotype of CHIKV and proposed as an alternative cell for the in vitro CHIKV studies to the commonly used A549 and Vero cells.

Co-Authors . DARMINTO . DARMINTO Ahmed, Ahmed Amarila Malik Anindya, Isti Anis Karuniawati ANOM BOWOLAKSONO Ariyanto, Ibnu Agus Aulia Rizki BENEDIKTUS YOHAN DIONISIUS DENIS Farida Dwi Handayani Fera Ibrahim Gasem, Muhammad Hussein GINA SAMAAN Hidajat, M Choirul Kambang Sariadji Kusumawati, R. Lia NI LUH PUTU INDI DHARMAYANTI NI LUH PUTU INDI DHARMAYANTI Ni Luh Putu Indi Dharmayanti Novipuspitasari, Lisa Oktaviani Naulita Turnip Pandia, Pandiaman Pratiwi Pujilestari Sudarmono Prawoto Prawoto R. TEDJO SASMONO RAHMA F. HAYATI Rina Yunita Rini Sekartini RISA INDRIANI RIZKA ALAWIYAH Safari, Dodi Sari, Novika Purnama Sembiring, Rosita J. Sinaga, Bintang YM. Sunarno Sunarno Tjahjani M. Sudiro Tjhin Wiguna Wahyuni, Arlinda S. Yamamoto, Zulham

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Journal : Microbiology Indonesia