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All Journal Proceedings of the International Conference on Applied Science and Health Global Health Management Journal GERVASI: Jurnal Pengabdian kepada Masyarakat Tunas Medika Jurnal Kedokteran & Kesehatan KOLONI
Amanah Amanah
Universitas Swadaya Gunung Jati
Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Education Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Uji Aktivitas Antibakteri Ekstrak n-Heksana, Etil Asetat dan Etanol Kulit Bawang Merah (Allium cepa L.) terhadap Pertumbuhan Bakteri Salmonella typhi ATCC 6539 Ferdiansyah Hakim; Hikmah Fitriani; Amanah Amanah
KOLONI Vol. 5 No. 2 (2026): JUNI 2026
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/koloni.v5i2.927

Abstract

Salmonella typhi is the causative agent of typhoid fever and remains a major public health problem in developing countries. Irrational use of antibiotics may lead to bacterial resistance, thus alternative herbal therapies are needed. Red onion peel (Allium cepa L.) contains secondary metabolites such as flavonoids, alkaloids, saponins, and tannins that potentially exhibit antibacterial activity. This study aimed to analyze the antibacterial activity of n-hexane, ethyl acetate, and ethanol extracts of red onion peel against the growth of Salmonella typhi ATCC 6539.This study was an in vitro laboratory experimental study using a post-test only control group design. The population consisted of Salmonella typhi ATCC 6539 bacteria, while the samples were pure bacterial cultures grown on Salmonella Shigella Agar (SSA). Samples were obtained using a simple random sampling technique. The study consisted of 14 groups, including positive control (0.1% chloramphenicol), negative control (10% DMSO), and treatment groups of n-hexane, ethyl acetate, and ethanol extracts at concentrations of 40%, 60%, 80%, and 100%. Data were collected using the well diffusion method by measuring inhibition zones with a caliper. Data analysis was performed using the Shapiro-Wilk test followed by One-Way ANOVA and Post Hoc Bonferroni test.Phytochemical screening showed that ethyl acetate and ethanol extracts contained flavonoids, alkaloids, saponins, and tannins, whereas the n-hexane extract only contained alkaloids and saponins. The 100% ethyl acetate extract produced the largest mean inhibition zone of 9.17 mm, while the 40% concentration produced the smallest mean inhibition zone of 6.53 mm. The 100% ethanol extract produced a mean inhibition zone of 8.58 mm, while the 40% concentration produced 6.77 mm. The n-hexane extract showed no inhibition zone at all concentrations. One-Way ANOVA analysis demonstrated significant differences among treatment groups (p<0.05).Ethyl acetate and ethanol extracts of red onion peel (Allium cepa L.) exhibited antibacterial activity against Salmonella typhi ATCC 6539 with moderate inhibitory strength, while the n-hexane extract showed no antibacterial activity. The 100% ethyl acetate extract was the most effective treatment in inhibiting bacterial growth.
TRANSFORMASI KESADARAN DEMAM BERDARAH DENGUE (DBD) MELALUI PENDEKATAN SOCIAL LEARNING DI DESA RAJAGALUH MAJALENGKA Amanah amanah; Yandri Naldi; Risnandya Primanagara; Firyal Amirah Hasna; Haura Febrianti Putri; Hayatun Nupus; Qothrunnada Syahira Ramadiani; Eva Riyanti
GERVASI: Jurnal Pengabdian kepada Masyarakat Vol. 10 No. 2 (2026): GERVASI: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM IKIP PGRI Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31571/gervasi.v10i2.10718

Abstract

Demam Berdarah Dengue (DBD) menjadi masalah utama di wilayah tropis. Salah satunya pada Kabupaten Majalengka terdapat 1.286 kasus pada tahun 2024. Tingginya kasus tersebut karena minimnya faktor yang berkontribusi terhadap risiko penularan, seperti tingkat pengetahuan dan sikap masyarakat dalam pencegahan DBD. Kondisi ini mendorong dilaksanakannya kegiatan penyuluhan yang bertujuan untuk menganalisis tingkat pengetahuan dan sikap mendukung upaya pencegahan DBD di Desa Rajagaluh, Kabupaten Majalengka. Kegiatan ini melalui penyuluhan  dan kuestioner dengan 35 peserta. Materi meliputi pengenalan DBD, upaya pencegahan melalui penerapan Perilaku Hidup Bersih dan Sehat (PHBS) dan program 3M. Setelah penyuluhan, peserta mengisi kuesioner pengetahuan dan sikap. Data dianalisis dengan uji korelasi Spearman. Hasil menunjukkan tingkat pengetahuan dan sikap dalam kategori cukup hingga baik (p value 0,02, r=0,39), serta terdapat hubungan positif antara pengetahuan dan sikap. Temuan ini menunjukkan bahwa strategi social learning dengan penyuluhan memberikan respon positif dalam perilaku knowledge-attitude gap pengendalian dan menurunkan risiko penularan DBD.
Co-Authors Ariestya Indah Permata Sari Atik Sutisna Dhini Oktaviani Donny Nauphar Eni Suhaeni Eva Riyanti Ferdiansyah Hakim Firyal Amirah Hasna Haura Febrianti Putri Hayatun Nupus Hikmah Fitriani Iwan Hermawan Marwan Hermawan Mega Ayu Lestari Mellyna Iriyanti Sujana Moch Yusuf Handoyo Naufal Fadhil Mufid Lazuardi Novianti Dwi Puspitasari Nurbaiti Nurbaiti Qothrunnada Syahira Ramadiani Rama Samara Brajawikalpa Ricardi Witjaksono Alibasjah Risnandya Primanagara Ronaa Hammada Rose Indriyati Sharah Julia Andayani Shofa Nur Fauzah Syahidatun Hayati Tissa Octavira Permatasari Witri Pratiwi Yandri Naldi