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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Jurnal Pendidikan Biologi Undiksha Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Biotechnology Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Agroplasma Jurnal Abdi Insani Journal of Marine and Coastal Science Jurnal Perikanan
Ketut Srie Marhaeni Julyasih
Universitas Pendidikan Ganesha
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Physics

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Genetic Diversity of Unhatched Sea Turtle Eggs in Buleleng, Bali Indonesia Hatchery Based on The Mitochondrial Control region Locus Immanuel Pratama; Ni Putu Dian Pertiwi; I Made Oka Riawan; Ni Luh Astria Yusmalinda; Andrianus Sembiring; Luh Putu Candra Apriliani; Ni Komang Rossa Sri Savitri; Srie Marhaeni Julyasih, Ketut; Jafron Syah, Moh; Rita Rachmawati
Journal of Marine and Coastal Science Vol. 14 No. 3 (2025): SEPTEMBER
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v14i3.77095

Abstract

Sea turtles are protected animals that are threatened with extinction due to natural and anthropogenic factors such as coastal erosion and illegal trade. This study aims to analyze the genetic diversity of sea turtles in Buleleng using mitochondrial DNA from unhatched egg samples. Samples were collected from two nests at several breeding sites to determine genetic variation within a single conservation area. Molecular analysis was conducted on the control region locus with a sequence length of 869 bp. The results of the identification showed that the samples originated from three species: green sea turtles (Chelonia mydas), olive ridley sea turtles (Lepidochelys olivacea), and hawksbill sea turtles (Eretmochelys imbricata), with sequence similarities of 99.44%–99.77%. The phylogenetic tree formed four clades with bootstrap values of 79–100%, supporting the classification and kinship relationships between species. Low intraspecific genetic distance (0–0.2%) and interspecific genetic distance (16.4–21.4%) indicate high similarity within a species and significant differences between species. The results of this study can be used to strengthen effective genetics-based sea turtle conservation programs in the Buleleng region of North Bali.
Analisis Molekuler Bakteri Liberobacter asiaticus Penyebab Penyakit Citrus Vein Phloem Degeneration (CVPD) dengan Teknik Polymerase Chain Reaction (PCR) pada Tanaman Jeruk Siam (Citrus nobilis) Parista, Ni Made Sudiyasih; Julyasih, Ketut Srie Marhaeni; Arnyana, Ida Bagus Putu
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.8372

Abstract

Salah satu komoditas buah utama Indonesia adalah jeruk Siam. Namun, banyak tanaman jeruk di indonesia yang terkena penyakit Citrus Vein Phloem Degeneration (CVPD). Penelitian ini bertujuan untuk mengidentifikasi secara lebih pasti keberadaan bakteri Liberobacter asiaticus penyebab penyakit CVPD pada tanaman jeruk yang menunjukkan gejala klorosis dan juga keberadaan bakteri L. asiaticus penyebab penyakit CVPD pada tanaman jeruk yang tidak menunjukkan gejala klorosis. Untuk mencapai hal tersebut, teknik PCR akan digunakan untuk analisis molekuler bakteri L. asiaticus. Dengan mengamati sampel daun dari tanaman jeruk siam Kintamani yang menunjukkan gejala klorosis dan yang tidak menunjukkan gejala klorosis, maka metodologi penelitian eksploratif diterapkan dalam penelitian ini. Pita DNA pada 1.160 bp tidak ditemukan pada sampel daun jeruk yang diuji, baik pada sampel daun jeruk yang sehat maupun pada sampel daun jeruk yang terindikasi klorosis. Tanaman jeruk yang menunjukkan gejala klorosis tidak ditemukan bakteri L. asiaticus pada sampel daunnya. Hal ini mungkin disebabkan oleh kurangnya unsur hara di dalam tanah, konsentrasi bakteri yang rendah, atau distribusi bakteri yang tidak merata di dalam jaringan daun. Kesimpulan yang didapat dari penelitian ini adalah keberadaan bakteri L. asiaticus penyebab penyakit CVPD tidak dapat terdeteksi keberadaannya pada sampel daun jeruk siam yang bergejala dan tidak bergejala klorosis.
Identification and Incidence of Rice Diseases (Oryza sativa L.) in Conventional Land in Penebel Village Rahayu, Diajeng Putri Ayu; Julyasih, Ketut Srie Marhaeni; Yuliastuti, Yuliastuti
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.10798

Abstract

Rice is a popular and widely consumed food crop in Indonesia, making disease a crucial factor in rice production. Penebel Village is a major rice producer, so disease damage significantly impacts rice productivity. This study was conducted to determine the types, characteristics, and incidence of rice plant diseases in conventional fields. The method used was exploratory descriptive, consisting of field surveys and interviews, as well as microscopic observations. Samples were taken using the quadrant method with five sampling points. Each point consisted of 16 plants, resulting in a total of 80 samples. Identification was performed using macromorphology. The rice plants observed were in the vegetative phase. The results of the study showed that in the rice fields were found Pyricularia sp. causing blast disease, Fusarium sp. causing fusarium wilt, Cercospora sp. causing narrow brown leaf spots, Helminthosporium sp. causing brown spots and Xanthomonas sp. causing bacterial leaf blight. The results of the calculation of disease incidence in conventional rice fields were blast (10%), fusarium (26.25%), leaf spots (16.25%), brown spots (51.25%), and bacterial leaf blight (50%). The results of the study indicate that the types, characteristics and incidence of diseases that appear in conventional agricultural land in Penebel village are very complex, so that more appropriate disease control is needed.
Efektivitas Biofungisida Jamur Trichoderma harzianum dan Trichoderma koningii dengan Berbagai Dosis terhadap Intensitas Serangan Penyakit Layu Jamur Fusarium pada Tanaman Cabai Merah (Capsicum annum L.) Sadin, Yuliana Ivantri; Julyasih, Ketut Srie Marhaeni; Manalu, Jean Nihana
JURNAL AGROPLASMA Vol 12, No 2 (2025): Vol 12, No 2 (2025): (JURNAL AGROPLASMA VOLUME 12 NO 2 TAHUN 2025)
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v12i2.8288

Abstract

Red chili is a horticultural crop that is already widely cultivated in Indonesia. The main constraint faced by farmers is the attack of plant pests, namely Fusarium wilt disease. One alternative control method is the use of biofungicides containing the fungi T. harzianum and T. koningii. This research was conducted in two different locations: isolation of the pathogenic fungus Fusarium, identification of Trichoderma fungi, and mass production of T. harzianum and T. koningii using rice medium at the Biological Agents / Botanical Pesticides Laboratory, Biaung. The planting of red chili seedlings and the application of biofungicides were carried out in the field of the Biological Agents / Botanical Pesticides Laboratory on Jalan Tegal Harum, Gang Sakura, Biaung, East Denpasar District, Denpasar City, Bali, from May to August 2025. The experimental design used was a Completely Randomized Design (CRD) with 7 treatments and 5 replications. The treatments were: 0 grams of biofungicide of T. harzianum and T. koningii fungi, a dose of 10 grams, a dose of 20 grams, a dose of 30 grams of T. harzianum biofungicide, and doses of 10 grams, 20 grams, and 30 grams of T. koningii biofungicide. Statistically, the application of different doses of T. harzianum and T. koningii biofungicides produced a significant difference in suppressing the intensity of Fusarium wilt disease, as shown by the one-way ANOVA test (p<0.05). The use of T. harzianum and T. koningii biofungicides at a dose of 30 grams proved effective against Fusarium wilt, with the level of suppression reaching 100% after four weeks of application. Keywords: Fusarium Wilt, red chili pepper, Trichoderma harzianum, Trichoderma koningii.
Differences in the Percentage of Fusarium Wilt Disease Attacks and High Vegetative Growth of Purple Eggplant (Solanum melongena L.) Plants with Various Concentrations of Antagonist Fungus Trichoderma harzianum Ketut Srie Marhaeni Julyasih; I Putu Parwata; Ni Putu Dian Pertiwi
Jurnal Penelitian Pendidikan IPA Vol 11 No 8 (2025): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i8.12090

Abstract

Microorganisms that have antagonistic properties against pathogens are an alternative as a material for control, such as Trichoderma sp. The use of biological agents has the potential to control plant diseases safely and environmentally friendly, Trichoderma sp is a fungus that can be a biocontrol agent because it is antagonistic to other fungi. Trichoderma sp is a fungus whose habitat is in the soil, including the Ascomycetes class which has green spores. This fungus has the potential for degradation of various heterogeneous substrates in the soil, positive interactions with the host, producing enzymes to improve plant nutrition. Until now, there has not been much research conducted on the competence of Trichoderma sp. isolates in inhibiting the development of Fusarium wilt disease. The purpose of this study was to test the difference in the percentage of Fusarium wilt disease attacks on eggplant plants and plant vegetative growth due to the provision of variations in the concentration of Trichoderma sp. The type of research conducted was a true experimental, with the research design used was a Completely Randomized Design using 6 treatments, namely with the treatment of Trichoderma sp. isolate concentration. grown on rice media with concentrations of 0%, 10%, 20%, 30% 40%, and 50%, each treatment was repeated 5 times so that there were 30 experimental units. The variables observed in this study were the differences in the percentage of Fusarium Wilt disease attacks and the high vegetative growth of purple eggplant plants. The data obtained were analyzed using the analysis of variance of the ANOVA test with a level of 5%. If there is a significant difference, it is continued with the Duncan test at a level of 5%. The results showed that there was a significant difference in the variation of T. harzianum concentration on the percentage of Fusarium wilt disease attacks, and the high growth of purple eggplant plants.
Co-Authors ., Monika Megawati Ferdiana Dara ., NI MADE DWI RIANI SARASWATI Andrianus Sembiring Arika Purnawati Bima Apry Anggara Desak Made Citrawathi Dwipayanti, Kadek Intan I GEDE PUTU WIRAWAN I Made Oka Riawan I NYOMAN WIJAYA I Putu Anom I Putu Parwata I Wayan Sukra Warpala Ida Ayu Putu Suryanti Ida Bagus Putu Arnyana Immanuel Pratama Kadek Intan Dwipayanti Luh Putu Candra Apriliani Manalu, Jean Nihana Monika Megawati Ferdiana Dara . Ni Komang Rossa Sri Savitri Ni Luh Astria Yusmalinda NI MADE DWI RIANI SARASWATI . Ni Putu Dian Pertiwi Ni Putu Dian Pertiwi Ni Putu Dian Pertiwi, Ni Putu Dian Ni Putu Nila Ristiari Ni Putu Ristiati Ni Putu Sri Ratna Dewi Ni Putu Ulan Rosita Putri NP Sri Ratna Dewi Parista, Ni Made Sudiyasih Rahayu, Diajeng Putri Ayu Ratna Dewi, NP Sri Ristiari, Ni Putu Nila Rita Rachmawati Rosita Putri, Ni Putu Ulan Sadin, Yuliana Ivantri WAYAN ADIARTAYASA Yuliastuti Yuliastuti