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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Jurnal Pendidikan Biologi Undiksha Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Biotechnology Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Abdi Insani Journal of Marine and Coastal Science Jurnal Perikanan
Ketut Srie Marhaeni Julyasih
Universitas Pendidikan Ganesha
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Physics

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Genetic Diversity of Unhatched Sea Turtle Eggs in Buleleng, Bali Indonesia Hatchery Based on The Mitochondrial Control region Locus Immanuel Pratama; Ni Putu Dian Pertiwi; I Made Oka Riawan; Ni Luh Astria Yusmalinda; Andrianus Sembiring; Luh Putu Candra Apriliani; Ni Komang Rossa Sri Savitri; Srie Marhaeni Julyasih, Ketut; Jafron Syah, Moh; Rita Rachmawati
Journal of Marine and Coastal Science Vol. 14 No. 3 (2025): SEPTEMBER
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v14i3.77095

Abstract

Sea turtles are protected animals that are threatened with extinction due to natural and anthropogenic factors such as coastal erosion and illegal trade. This study aims to analyze the genetic diversity of sea turtles in Buleleng using mitochondrial DNA from unhatched egg samples. Samples were collected from two nests at several breeding sites to determine genetic variation within a single conservation area. Molecular analysis was conducted on the control region locus with a sequence length of 869 bp. The results of the identification showed that the samples originated from three species: green sea turtles (Chelonia mydas), olive ridley sea turtles (Lepidochelys olivacea), and hawksbill sea turtles (Eretmochelys imbricata), with sequence similarities of 99.44%–99.77%. The phylogenetic tree formed four clades with bootstrap values of 79–100%, supporting the classification and kinship relationships between species. Low intraspecific genetic distance (0–0.2%) and interspecific genetic distance (16.4–21.4%) indicate high similarity within a species and significant differences between species. The results of this study can be used to strengthen effective genetics-based sea turtle conservation programs in the Buleleng region of North Bali.
Differences in the Percentage of Fusarium Wilt Disease Attacks and High Vegetative Growth of Purple Eggplant (Solanum melongena L.) Plants with Various Concentrations of Antagonist Fungus Trichoderma harzianum Julyasih, Ketut Srie Marhaeni; Parwata, I Putu; Pertiwi, Ni Putu Dian
Jurnal Penelitian Pendidikan IPA Vol 11 No 8 (2025): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i8.12090

Abstract

Microorganisms that have antagonistic properties against pathogens are an alternative as a material for control, such as Trichoderma sp. The use of biological agents has the potential to control plant diseases safely and environmentally friendly, Trichoderma sp is a fungus that can be a biocontrol agent because it is antagonistic to other fungi. Trichoderma sp is a fungus whose habitat is in the soil, including the Ascomycetes class which has green spores. This fungus has the potential for degradation of various heterogeneous substrates in the soil, positive interactions with the host, producing enzymes to improve plant nutrition. Until now, there has not been much research conducted on the competence of Trichoderma sp. isolates in inhibiting the development of Fusarium wilt disease. The purpose of this study was to test the difference in the percentage of Fusarium wilt disease attacks on eggplant plants and plant vegetative growth due to the provision of variations in the concentration of Trichoderma sp. The type of research conducted was a true experimental, with the research design used was a Completely Randomized Design using 6 treatments, namely with the treatment of Trichoderma sp. isolate concentration. grown on rice media with concentrations of 0%, 10%, 20%, 30% 40%, and 50%, each treatment was repeated 5 times so that there were 30 experimental units. The variables observed in this study were the differences in the percentage of Fusarium Wilt disease attacks and the high vegetative growth of purple eggplant plants. The data obtained were analyzed using the analysis of variance of the ANOVA test with a level of 5%. If there is a significant difference, it is continued with the Duncan test at a level of 5%. The results showed that there was a significant difference in the variation of T. harzianum concentration on the percentage of Fusarium wilt disease attacks, and the high growth of purple eggplant plants.
Analisis Molekuler Bakteri Liberobacter asiaticus Penyebab Penyakit Citrus Vein Phloem Degeneration (CVPD) dengan Teknik Polymerase Chain Reaction (PCR) pada Tanaman Jeruk Siam (Citrus nobilis) Parista, Ni Made Sudiyasih; Julyasih, Ketut Srie Marhaeni; Arnyana, Ida Bagus Putu
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.8372

Abstract

Salah satu komoditas buah utama Indonesia adalah jeruk Siam. Namun, banyak tanaman jeruk di indonesia yang terkena penyakit Citrus Vein Phloem Degeneration (CVPD). Penelitian ini bertujuan untuk mengidentifikasi secara lebih pasti keberadaan bakteri Liberobacter asiaticus penyebab penyakit CVPD pada tanaman jeruk yang menunjukkan gejala klorosis dan juga keberadaan bakteri L. asiaticus penyebab penyakit CVPD pada tanaman jeruk yang tidak menunjukkan gejala klorosis. Untuk mencapai hal tersebut, teknik PCR akan digunakan untuk analisis molekuler bakteri L. asiaticus. Dengan mengamati sampel daun dari tanaman jeruk siam Kintamani yang menunjukkan gejala klorosis dan yang tidak menunjukkan gejala klorosis, maka metodologi penelitian eksploratif diterapkan dalam penelitian ini. Pita DNA pada 1.160 bp tidak ditemukan pada sampel daun jeruk yang diuji, baik pada sampel daun jeruk yang sehat maupun pada sampel daun jeruk yang terindikasi klorosis. Tanaman jeruk yang menunjukkan gejala klorosis tidak ditemukan bakteri L. asiaticus pada sampel daunnya. Hal ini mungkin disebabkan oleh kurangnya unsur hara di dalam tanah, konsentrasi bakteri yang rendah, atau distribusi bakteri yang tidak merata di dalam jaringan daun. Kesimpulan yang didapat dari penelitian ini adalah keberadaan bakteri L. asiaticus penyebab penyakit CVPD tidak dapat terdeteksi keberadaannya pada sampel daun jeruk siam yang bergejala dan tidak bergejala klorosis.
Co-Authors ., Monika Megawati Ferdiana Dara ., NI MADE DWI RIANI SARASWATI Andrianus Sembiring Arika Purnawati Bima Apry Anggara Desak Made Citrawathi Dwipayanti, Kadek Intan I GEDE PUTU WIRAWAN I Made Oka Riawan I NYOMAN WIJAYA I Putu Anom I Putu Parwata I Wayan Sukra Warpala Ida Ayu Putu Suryanti Ida Bagus Putu Arnyana Immanuel Pratama Kadek Intan Dwipayanti Luh Putu Candra Apriliani Monika Megawati Ferdiana Dara . Ni Komang Rossa Sri Savitri Ni Luh Astria Yusmalinda NI MADE DWI RIANI SARASWATI . Ni Putu Dian Pertiwi Ni Putu Dian Pertiwi, Ni Putu Dian Ni Putu Nila Ristiari Ni Putu Ristiati Ni Putu Sri Ratna Dewi Ni Putu Ulan Rosita Putri NP Sri Ratna Dewi Parista, Ni Made Sudiyasih Ratna Dewi, NP Sri Ristiari, Ni Putu Nila Rita Rachmawati Rosita Putri, Ni Putu Ulan WAYAN ADIARTAYASA