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Antifungal Activity of Endophytic Bacteria Isolated from Dayak Onion (Eleutherine bulbosa) Against Candida albicans Krestina, Widya; Mutiasari, Dian; Wardhana, Vinsen Willi; Zulviana; Rahmawati, Maynissa; Naibaho, Frans Grovy; Panjaitan, Desimaria
Jurnal Sumberdaya Hayati Vol. 10 No. 4 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.4.157-161

Abstract

Candidiasis is a fungal infection due to a decrease in the immune system caused by Candida spp. In general, candidiasis is treated with antifungal drugs, but uncontrolled use can result in the emergence of drug resistance and toxicity. Endophytic bacteria have the potential to be biological control agents against various fungal infections including candidiasis. This study aims to determine the antagonistic activity of endophytic bacteria isolated from Dayak onions and the antifungal activity of its cell-free supernatant (CFS) against Candida albicans. Thirteen isolate strains of endophytic bacteria were used in this study. Antagonistic activity was tested using the agar plug diffusion method. Endophytic bacteria that showed antagonistic activity against C. albicans were cultivated in Nutrient Broth media to produce CFS. The antifungal activity of CFS was tested using the Kirby–Bauer disk diffusion method. The results indicated that six isolates of endophytic bacteria from dayak onions have antagonistic activity against C. albicans. CFS from strain CED4 has a strong antifungal activity against C. albicans with a clear zone diameter of 18.5 mm. Further studies are needed to determine the antifungal compounds produced by CED4 isolates for the development of anticandidal.
Evaluation of Antagonistic Activity of Endophytic Bacteria from Dayak Onion Against Ganoderma boninense Desiana Sinta; Norwahyunie Norwahyunie; Noraini Noraini; Zulviana Zulviana; Frans Grovy Naibaho; Vinsen Willi Wardhana; Desimaria Panjaitan; Adrian Hartanto
Bioactivities Vol. 2 No. 2 (2024): Bioactivities
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/bioactivities.2963-654X.239

Abstract

Basal stem rot (BSR) is one of the key contributors to the decline in palm oil production in Indonesia. BSR is caused by the pathogenic plant fungus Ganoderma boninense. Currently, the control of G. boninense growth relies on systemic fungicides. However, systemic fungicides have negative impacts as they can harm natural soil microorganisms. Biocontrol agents present an environmentally friendly alternative for G. boninense management. The objective of this research was to select and identify endophytic bacterial isolates from Dayak onion [Eleutherine bulbosa (Mill.) Urb.] with antagonistic activity against G. boninense. The investigation was initiated with the isolation of 34 endophytic bacterial isolates and G. boninense isolate. The hemolytic capabilities of endophytic bacterial isolates were evaluated on blood agar media. Antagonism assessments were conducted through the dual-culture method, and the inhibition of G. boninense was measured using the percentage inhibition of radial growth (PIRG). The endophytic bacteria exhibiting the highest PIRG values were identified through molecular analysis based on the 16S rRNA gene sequence. Results revealed that 27 isolates demonstrated no ability to hydrolyze blood agar (gamma hemolysis) indicating no pathogenicity. Isolate CED9 demonstrated the highest PIRG value at 70.26%. Genomic identification based on the 16S rRNA gene sequence confirmed that isolate CED9 was classified as Bacillus subtilis. The identification of biocontrol agents from Dayak onion represents valuable information and a potential arsenal for future strategies in mitigating (BSR) disease.
Pelatihan Pemanfaatan Limbah Baglog Jamur Tiram Menjadi Pupuk Multi Fungsi di Kota Palangka Raya: Training on Utilization of Oyster Mushroom Baglog Waste into Multi-Function Fertilizer in Palangka Raya Saraswati, Dewi; Nion, Yanetri Asi; Mulyani, Rahmawati Budi; Kamillah, Kamillah; Djaya, Adrianson Agus; Sari, Siniy Kumala; Wardhana, Vinsen Willi
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. Suppl-1 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10iSuppl-1.8666

Abstract

From July to September 2024, a community service team from the Agrotechnology Study Program, Faculty of Agriculture, University of Palangka Raya, together with partners, conducted training on utilizing oyster mushroom baglog waste into multi-function fertilizer for mushroom farmers in Palangka Raya. The first partner is the Indonesian Phytopathology Association Central Kalimantan Regional Commissariat (PFI Komda Kalteng) and the second is mushroom farmers in Palangka Raya. The methods used include preparation, counseling, mentoring, and demonstrations, as well as training in Trichoderma propagation, making Trichocompost, compost packaging, and using compost as a planting media. Additionally, demonstration plots are made using Trichocompost. The mushroom farmers have succeeded in mastering the technique of making Trichocompost from oyster mushroom baglog waste. This innovation not only reduces environmental waste but also makes oyster mushroom cultivation more sustainable. Waste that is usually thrown away or only used as direct fertilizer is now processed into a multifunction fertilizer that can stimulate plant growth and increase plant resistance to disease that proven by a demonstration plot of chili cultivation using Trichokompos. This processing process not only increases the efficiency of natural resource use but also provides added value to waste and increases mushroom farmers' income.
Keanekaragaman Jamur Makroskopis di Kawasan Hutan Universitas Palangka Raya Kalimantan Tengah Panjaitan, Desimaria; Wardhana, Vinsen Willi; Febiolasari, Santi Dwi
Jurnal Kajian Ilmiah Vol. 22 No. 2 (2022): May 2022
Publisher : Lembaga Penelitian, Pengabdian Kepada Masyarakat dan Publikasi (LPPMP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31599/hazg7p13

Abstract

Macroscopic fungi comprise a very diverse group of heterotrophic and mostly saprophytic organisms. This research purpose was to identify the diversity of macroscopic fungi from the forest of University of Palangka Raya. The fungi samples were collected from May until June 2021 using purposive sampling method. The samples were identified based on macroscopic characters such as the form of fruiting bodies, lamellae, cap shape and colours. There were 18 species of Basidiomycota which consist of 9 famili and 12 genera. The species were identified as Agrocybe sp., Auricularia sp.1, Auricularia sp.2, Collybia sp., Dacryopinax spathularia, Ganoderma sp.1, Ganoderma sp.2, Ganoderma sp.3, Hygrocybe sp., Marasmiellus sp., Mycena sp.1, Mycena sp.2, Pycnoporus sanguineus, Schizophyllum commune, Trametes sp.1, Trametes sp.2, Trametes sp.3, and Tyromyces sp. Descriptions of each species were provided.
Pelatihan Budidaya Cabai Rawit untuk Kebutuhan Rumah Tangga di Provinsi Kalimantan Tengah: Training of Cayenne Pepper Cultivation for Household Needs in Central Kalimantan Province Nion, Yanetri Asi; Kamillah, Kamillah; Mulyani, Rahmawati Budi; Supriati, Lilies; Djaya, Adrianson Agus; Saraswati, Dewi; Pandriyani, Pandriyani; Damanik, Zafrullah; Sinaga, Soaloon; Wardhana, Vinsen Willi; Erniaty, Erniaty
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 7 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i7.9127

Abstract

Cayenne pepper cultivation training to household needs is a collaboration between the Agrotechnology study program, Faculty of Agriculture, University of Palangka Raya with the Indonesian Phytopathology Association, Regional of Central Kalimantan, which has been carried out from April to December 2024 in the city of Palangka Raya and Bukit Bamba Village. The training was in counseling and also cultivation guidance through field visits for people who received chili seeds. Eighty percent of the plants distributed grew well, where 40% of the fruit could be consumed for household needs, and the cause of the failure of the chili harvest was that the plants were stolen, there were pest and disease attacks, and lack of patience or lack of interest in caring for the plants. The average successful chili harvest production per harvest per tree per week was 39 grams. This activity not only includes the distribution of cayenne pepper seeds, but also has a positive impact on the community. From the data obtained, as many as 82.14% of chili plants grew well, while 17.86% were lost, either due to theft or pest and disease attacks. The first harvest showed that 78.26% of the harvested chilies had been used for household needs, while the remaining 21.74% were still waiting for the harvest period. In terms of production, the average harvest per tree reached 39 grams, with the lowest yield of 18 grams and the highest of 66 grams. This achievement shows the great potential of cayenne pepper cultivation in increasing food security.
Pendampingan Pembuatan Produk Usaha BUIS (Bubuk Ikan Seluang) sebagai Bahan Tambahan pada Makanan untuk Mencegah Stunting Rosmainar, Lilis; Yuliana, Yuliana; Wardhana, Vinsen Willi
Jurnal ABDINUS : Jurnal Pengabdian Nusantara Vol 9 No 3 (2025): Volume 9 Nomor 3 Tahun 2025
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/ja.v9i3.24285

Abstract

Stunting is defined as a disruption in a child's growth and development. The prevalence of stunting must be continuously reduced. Therefore, various efforts are being made, such as providing adequate nutrition. One food source that is high in protein and high in calcium is the seluang fish (Rasbora Argyrotaenia). The majority of residents living along the river in Palangka Raya earn their living as fishermen. This fish is widely found and sold in the market, making it quite affordable. To increase the market value of this fish, innovations have been made in processing seluang fish, namely by making seluang fish powder. The processing method involves boiling the fish in a pressure cooker, drying it in an oven, and then grinding it into a powder. The resulting fish powder is blackish in color and has a distinctive odor. Training on making fish powder was conducted for residents living along the river, attended by 30 residents, and the participants were very enthusiastic about the activity.