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All Journal Jurnal Veteriner Journal Of Vocational Health Studies Vitek : Bidang Kedokteran Hewan Darmabakti Cendekia: Journal of Community Service and Engagements Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Applied Veterinary Science and Technology Jurnal Kedokteran Hewan

Agung Budianto Achmad, Agung Budianto

Departemen Kedokteran Dasar, Fakultas Kedokteran Hewan, Universitas Airlangga

Author-ID : 3900685

Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Terapi Sel Punca Mesenkimal Sumsum Tulang Tikus dalam Meregenerasi Sel Sitotrofoblas Nekrosis yang Dipapar Carbon Black (RAT BONE MARROW MESENCHYMAL STEM CELL THERAPY IN REGENERATING NECROTIC CYTOTROPHOBLAST CELL FOLLOWING EXPOSED TO CARBON BLACK) Widjiati .; Sri Pantja Madyawati; Rimayanti .; Agung Budianto Achmad
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.727 KB)

The objective of this study is to find out the potency of Rat Bone Marrow Mesenchymal Stem Cell(RBMMSC) in regenerating necrotic cytotrophoblast cells of rats (Rattusnorvegicus) following exposure tocarbon black at day 6 of gestation at different time of exposure (6 days and 12 days). This study usedrandomized factorial design with two factors (gestation day and treatment). Forty-eight gravid femalerats were divided into six treatment groups i.e. (i) animals at day 6-11 gestation and not expose to carbonblack; (ii) 6-11 days gestation animals + 532mg/m3 carbon black for 4 hours; (iii) 6-11 days gestationanimals + 532mg/m3 carbon black for 4 hours +1x107/0.1ml RBMMSC intravenously; (iv) animals at day6-17 gestation and not expose to carbon black; (v) 6-17 days gestation animals + 532mg/m3 carbon blackfor 4 hours; (vi) 6-17 days gestation animals + 532mg/m3 carbon black for 4 hours +1x107/0.1ml RBMMSCintravenously, respectively. Data were analyzed using univariat analysis and analysis of variance. Theresults showed that there were no significance differences in regenerating necrotic cytotrophoblast betweenthe groups treated with RBMMSC and carbon black exposure. The results indicated that the stem celltherapy following exposure to carbon black was incapable in regenerating the necrotic cytotrophoblastcells.

TRAINING OF MAKING FEED SUPPLEMENT OF UMB HERBAL IN PARENGAN, TUBAN REGENCY Siti Eliana Rochmi; Herinda Pertiwi; Agung Budianto Achmad; Retno Sri Wahjuni; Tri Wahyu Suprayogi; Tri Bhawono Dadi
Darmabakti Cendekia: Journal of Community Service and Engagements Vol. 2 No. 1 (2020): JUNE 2020
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Background: Subdistrict Parengan has high livestock potential especially Suciharjo Village and Margorejo Village. The existing agricultural waste is not treated properly and livestock cannot reach maximum weights. The provision of quality animal feed is one of the efforts to improve the welfare of the community. Quality of animal feed can be increasing cattle productivity so that the selling value will increase. Purpose: This Community Service activity aims to improve the economic strengthening of the community through breeders through the introduction, dissemination, through technology UMB Herbal based on local resources that can increase the weight gain of beef cattle. UMB Herbal is a concentrate feed that uses raw materials based on raw materials (agriculture) with curcuma which is widely available in the regions. Improving skills makes UMB Herbal can be a provision for the community to be able to increase income. Methods: The method used in community service is the counseling for animal feed and health, training to make UMB Herbal and focus group discussions then applicating of UMB Herbal to pilot cattle. Results: The results of this community service include an increase in beef cattle body weight, decreasing the gastrointestinal worm infestations, and increasing the knowledge and skills of the participants of the community service. Conclusion: Community Service Activities have a positive impact related to the development of beef cattle farms.

PELATIHAN PEMBUATAN DODOL TEMULAWAK UNTUK PENGGEMUKAN SAPI POTONG DI DESA CENGKONG DAN DESA BRANGKAL KECAMATAN PARENGAN KABUPATEN TUBAN Retno Sri Wahjuni; Siti Eliana Rochmi; Agung Budianto Achmad
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 3 No. 2 (2019): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Tujuan pengabdian masyarakat ini adalah mengaplikasikan teknologi pembuatan pakan berupa dodol temulawak sebagai upaya untuk menggemukkan sapi potong di Desa Brangkal dan Desa Cengkong Kecamatan Parengan Kabupaten Tuban. Rendahnya pakan yang diberikan dan banyaknya hasil sampingan pertanian yang belum diolah secara maksimal merupakan masalah utama dari kedua mitra. Solusi permasalahan mitra adalah dengan inovasi dodol temulawak yaitu pakan tambahan pengganti konsentrat yang mempunyai kandungan sumber energi dan protein yang baik. Bahan aditif yang digunakan adalah temulawak. Tanaman temulawak merupakan tanaman yang banyak ditanam oleh masyarakat Indonesia khususnya di wilayah mitra. Bahan pembuatan dodol temulawak menggunakan sumber daya lokal yang belum dimanfaatkan secara optimal oleh masyarakat. Metode yang dilakukan dengan pemberdayaan peternak yang diarahkan pada kegiatan peningkatan daya saing dan partisipasi masyarakat melalui peningkatan kapasitas dan kelembagaan SDM peternak dan kelompok ternakdengan peningkatan pengetahuan dan pelatihan pembuatan dodol temulawak. Hasil pengabdian ini menunjukkan hasil positif terhadap pertambahan berat badan sapi potong, dan meningkatkan sumber daya peternak baik secara pengetahuan maupun ketrampilan.ABSTRACTThe aim of Public service is to apply feed-making technology in the form of C. Xanthorrhiza, Roxb dodol as an effort to fattening beef cattle in Brangkal Village and Cengkong Village, Parengan Sub-District, Tuban Regency. The low feed provided and the number of agricultural by-products that have not been optimally treated are the main problems of the partners. The solution for partners problem is the innovation of C. Xanthorrhiza, Roxb dodol which is an additional feed substitute for concentrate which has good energy and protein sources. A lot of C. Xanthorrhiza, Roxb are found in Indonesia. It’s material used are based on local by-product that have not been used optimally. The method is done by empowering farmers who are directed to activities to increase competitiveness and community participation through increasing the capacity and institutional human resourcesof farmers and farmers community by increasing knowledge and training in the manufacture of C. Xanthorrhiza, Roxb dodol. The results of this dedication show positive results on weight gain of beef cattle, and increase the resources of farmers both in terms of knowledge and skills.

The Incidence of Helminthiasis in Cattle in The Work Area of UPT. Laboratorium Kesehatan Hewan in East Java Province in 2017-2018 Aulia Nur Afifah; Iwan Syahrial Hamid; Mirni Lamid; Agung Budianto Achmad
Journal of Applied Veterinary Science And Technology Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V1.I1.2020.16-23

Background: The needs of Indonesian people for animal protein from meat are still quite high, but the fulfillment of meat demand has not been sufficient. Many obstacles faced by farmers in developing their livestock business, one of which is helminthiasis. Helminth infections can occur due to feed problems or poor maintenance management. Helminth infestation in the digestive tract can cause intestinal mucosal damage, which can reduce the efficiency of food absorption, causing livestock productivity losses in body weight, milk production, quality of meat, skin, and innards. Purpose: This study aimed to determine the prevalence of helminthiasis and the type of helminths that infected cattle in UPT. Laboratorium Kesehatan Hewan Provinsi Jawa Timur. Method: This study used sedimentation and Whitlock floating method. Result: The highest prevalence in 2017 occurred in Tuban City, which was 6,69%, while the lowest was in Gresik Regency, which was 0%. Whereas in 2018, the highest prevalence occurred in Lamongan City, which was 9,42% and the lowest prevalence in Ngawi Regency, which was 0,22%. The type of helminth that infects the cattle the most in 2017 is Fasciola sp. as many as 114 cases, with the highest number occurring in Magetan Regency, which was 44 cases. In 2018, Fasciola sp. also the most common type of helminth found to infect cattle in 202 cases, with the highest number occurring in Magetan and Pacitan Regency, which was 34 cases.

The Effect of Molasses Addition on Physical and Chemical Quality of Corn Plant Silage Given Fermented Mother Liquor Nidia Yulanda; Nove Hidajati; Agung Budianto Achmad; Dony Chrismanto
Journal of Applied Veterinary Science And Technology Vol. 2 No. 1 (2021): April 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V2.I1.2021.10-14

Background: Making silage added with additives can improve the quality of silage nutrition and speed up the fermentation process. Molasses are widely used by farmers as additives for making silage. The use of molasses can be replaced with Fermented Mother Liquor (FML) in making silage. Purpose: to determine the effect of molasses addition on physical and chemical quality of corn plant silage given Fermented Mother Liquor (FML). Method: Observative data collection by making direct observations on making silage with two treatments consist of P0 (Corn Plant + 2% Fermented Mother Liquor (FML)) and P1 (Corn Plant + 2% Fermented Mother Liquor (FML) + 2% Molasses). The silage storage time was one week and on the seventh day observations were made on the physical and chemical quality of silage in each treatment. Result: The corn plant silage given fermented mother liquor (FML) without the addition of molasses produced better physical and chemical quality compared to corn plant silage given Fermented Mother Liquor (FML) with the addition of molasses. Conclusion: the corn plant silage given Fermented Mother Liquor (FML) only had better physical and chemical quality

Evaluation of Production Peak of Laying Hens Strain Lohman Brown in CV. Lawu Farm Malang Yolifia Rahma Milenia; Sri Pantja Madyawati; Agung Budianto Achmad; Ratna Damayanti
Journal of Applied Veterinary Science And Technology Vol. 3 No. 1 (2022): April 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V3.I1.2022.12-17

Background: The productivity of laying hens is a key measurement in the laying hens business. One of the targets for laying hens is to achieve peak production. The optimal peak production period is necessary to attain the maximum profit. Purpose: To determine the productivity of laying hens strain Lohman Brown aged 25-49 weeks at CV. Lawu Farm Malang Regency as an evaluation of the peak production period. Case Analysis: The descriptive method with the case study technique was used. Primary data were obtained from observations by participating in direct livestock activities, interviews, and documentation, and secondary data were obtained from literature studies. The variables observed in this study were Hen Day Production, Feed Conversion Ratio, egg weight, and mortality. Results: Observation of laying hens productivity in CV. Lawu Farm showed that 71.42% of Hen Day Production value of the full cages had met the target with HDP of more than 92%, while 28.58% did not reach the peak production target by only getting HDP less than 90%. The FCR value was 2.4 – 2.15, the average egg weight wais 63.03 grams/egg, and the mortality was ≤3%. Conclusion: The productivity of laying hens of the Lohman Brown strain aged 24-59 weeks at CV. Lawu Farm has met production standards in terms of HDP, FCR, egg weight, and mortality.

In Vitro Cytotoxicity Test of Red Okra (Abelmoschus esculentus) Fruit Ethanolic Extract on HeLa Cells Agung Budianto Achmad
Journal of Applied Veterinary Science And Technology Vol. 3 No. 1 (2022): April 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V3.I1.2022.22-26

Background: Cancer is one of the leading causes of death in pets. Among various therapeutic methods, chemotherapy is still the main choice to treat advanced and metastatic cancers. However, because of its non-target-specific mode of action, chemotherapy often causes severe side effects in patients. Purposes: This study aimed to analyze the cytotoxic effect of red okra fruit extract (Abelmoschus esculentus) on HeLa cells with a comparison of several incubation periods of therapy. Methods: Red okra fruit was extracted by the maceration method using 96% ethanol. Then its cytotoxic potency was tested in vitro on HeLa cells based on the MTT tetrazolium reduction assay. Results: The results of the MTT test showed a decrease in the viability of HeLa cells along with an increase in the concentration of red okra extract (400, 800, dan 1,600 ppm), where the highest percentage of cell inhibition was obtained at a concentration of 1,600 ppm for each group of 24, 48, and 72 hours of therapy. The lowest IC50 value was obtained by the 48-hour therapy group, which was 631.78 ppm, indicating the optimal duration of therapy of red okra against HeLa cells. Conclusion: The ethanolic extract of red okra (Abelmoschus esculentus) has an anticancer effect by inhibiting the growth and proliferation of HeLa cells. Thus, red okra fruit has potential benefits in the management of animals diagnosed with cancer.

Quality and Quantity of Dairy Cow Milk Production in View of Differences in Concentrate Use at KUD Kertajaya Kandangan, Kediri Regency, East Java Oriza Cahya Rahmadiati; Siti Eliana Rochmi; M. Gandul Atik Yuliani; Anwar Ma'ruf; Miyayu Soneta Sofyan; Agung Budianto Achmad; Dony Chrismanto
Journal of Applied Veterinary Science And Technology Vol. 4 No. 1 (2023): April 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V4.I1.2023.11-14

Background: Differences in the use of concentrates in dairy cows cause differences in the quantity and quality of milk produced. Purpose: To compare the quality and quantity of milk between dairy cows fed concentrate A and concentrate B. Methods: This observation was carried out at KUD Kertajaya Kandangan Kediri using 40 cows with a ratio of 20 dairy cows fed concentrate A and 20 cows dairy cows fed concentrate B. The quality of cow's milk samples was tested using a lactoscan machine, and then the quantity was measured at the time of milking using a scaled milk can. Results: The results showed that the quality of dairy cows fed with concentrate A had an average pH of 6.495±0.176, milk specific gravity of 1,0241±0.0004 g/ml, a fat content of 5,241±2.614%, protein of 3,335±0.294%, non-solid fat of 8.542±0.518% and total solid of 13.707±2.612% with an average milk yield of 15.7±2.273 liters per day while the quality of dairy cows fed with concentrate B had an average pH of 6.425±0.102, milk Specific Gravity of 1,0244±0.0005 g/ml, Fat Content of 6,447±2.621%, Protein of 3.284±0.213%, non-solid fat of 9.134±0.6313% and total solid of 15.459±2.752%% with the average milk produced is 15.5±2.140 liters per day. Conclusion: The quality and quantity of milk of dairy cows fed concentrates A and B have a noticeable difference (P<0.05) in terms of solid nonfat (SNF) and total solids. Concentrate B has a higher percentage of solid nonfat and total solid than concentrate A.

EFEKTIVITAS TERAPI RAT BONE MARROW MESENCHYMAL STEM CELL PADA TIKUS (Rattus norvegicus) MODEL TERATOGENIK PARTICULATE MATTER TERHADAP EKSPRESI TNF- , BAX, DAN BCL-2 PLASENTA Sri Pantja Madyawati; Rimayanti R; Widjiati W; Agung Budianto Achmad
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Tujuan penelitian ini adalah membuktikan potensi dan efektivitas rat bone marrow mesenchymal stem cell (rbmmsc) sebagai terapi pada kasus teratogenik selama kebuntingan dengan melihat ekspresi tumor necrosis factor (TNF-α), Bax, dan Bcl-2. Dua puluh empat ekor tikus bunting model teratogenik dibagi menjadi empat kelompok perlakuan yang berbeda yaitu P1-Kontrol (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-11 + Injeksi MEM 0,1 ml), P1-Terapi (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-11 + rbmmsc dengan dosis 1x106 sel/0,1 ml), P2-Kontrol (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umur kebuntingan ke-6 s/d ke-17 + Injeksi MEM 0,1 ml), P2-Terapi (dipapar carbon black dosis 532 mg/m3 selama 4 jam pada umurkebuntingan ke-6 s/d ke-11 + rbmmsc dengan dosis 1x106 sel/0,1 ml). Data yang didapatkan kemudian dianalisis menggunakan uji MannWhitney. Hasil penelitian ini menunjukkan bahwa pemberian terapi rat bone marrow mesenchymal stem cell pada tikus model teratogenikparticulate matter tidak berpengaruh dalam menurunkan parameter ekspresi TNF-α dan Bax, serta meningkatkan ekspresi Bcl-2 pada plasenta.

STEM CELL THERAPY ON ANIMAL MODEL (Rattus norvegicus) END GESTATIONAL TIME EXPOSED TO CARBON BLACK AS OBSERVED FROM CASPASE-3 EXPRESSION Achmad, Agung Budianto; Madyawati, Sri Pantja; Widjiati, Widjiati
Journal of Vocational Health Studies Vol. 1 No. 3 (2018): March 2018 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V1.I3.2018.102-106

Background: Air pollution in the form of Diesel Exhaust Particles emerging from motor vehicles are harmful to health that have adverse reproductive health impacts, especially during pregnancy. The use of stem cells in treating white mice (Rattus norvegicus) exposed to carbon black can reveal the potential for treatment of placental impairment during pregnancy. Purpose: to demonstrate the effectivity of Rat Bone Marrow Mesenchymal Stem Cell therapy on rats (Rattus norvegicus) exposed to carbon black as observed from caspase-3 expression. Method: This research uses a completely randomized design with factorial pattern. Forty-eight gravid female rats were divided into six treatment groups. Result: caspase-3 expression in each treatment showed no significant differences in the groups treated with RBMMSC in each gravid groups treatment (therapy GD 11 and GD 17) were exposed to carbon black and not treated with RBMMSC. The same are indicated by the normal trophoblast cells (cytotrophoblast and syncytiotrophoblast cells) in the RBMMSC treated group showed no significant difference with the group exposed to carbon black only. Conclusion: this research indicate that Rat Bone Marrow Mesenchymal Stem Cell therapy in Rattus norvegicus exposed to carbon black have not been able to reduce expression of caspase-3.

Co-Authors Anwar Ma'ruf Aulia Nur Afifah Chrismanto, Dony Dio Yudistira Diyantoro Diyantoro, Diyantoro Gandul Atik Yuliani Herinda Pertiwi Iwan Syahrial Hamid Meilinia, Shafina Meilinia Mirni Lamid Nidia Yulanda Nove Hidajati Oriza Cahya Rahmadiati Ratna Damayanti Retno Sri Wahjuni Rimayanti . Rimayanti R Rimayanti R, Rimayanti Rochmi, Siti Eliana Sofyan, Miyayu Soneta Sri Pantja Madyawati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Sri Pantja Madyawati, Sri Pantja Tri Bhawono Dadi Tri Wahyu Suprayogi Widjiati . Widjiati w Widjiati W Widjiati Widjiati, Widjiati Yolifia Rahma Milenia

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