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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Mutiara Medika: Jurnal Kedokteran dan Kesehatan The Indonesian Biomedical Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Journal of Global Pharma Technology Indonesian Journal of Biomedicine and Clinical Sciences
Usi Sukorini
Departement Of Clinical Pathology And Medical Laboratory, Faculty Of Medicine, Public Health And Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Journal : The Indonesian Biomedical Journal

 1 
High Expression of FcγII (CD32) Receptor on Monocytes in Dengue Infected Patients Umi Solekhah Intansari; Harina Salim; Usi Sukorini; Adika Zhulhi Arjana; Muhammad Juffrie
The Indonesian Biomedical Journal Vol 10, No 3 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i3.434

Abstract

BACKGROUND: Pathogenesis of severe dengue infection has not been elucidated. Immune complex of pre-existing antibodies and heterotypic dengue virus bind to FcγII (cluster of differentiation (CD32)) receptor (FcγIIR) on monocyte facilitates entry and replication of dengue virus. Aim of this study was to evaluate the expression of FcγIIR on monocytes in patients infected with dengue and in healthy subjects.METHODS: This study used a cross-sectional design that included patients infected with dengue who were hospitalized in Dr. Sardjito General Hospital, Panembahan Senopati Hospital, and Sleman Hospital, who met the inclusion criteria and selected consecutively. Examinations were completed using a lyse, no-wash method of flow cytometry. Computerized statistical analysis was conducted and was considered to be significant if p<0.05.RESULTS: Sixty-five study subjects were divided into healthy subjects (24 subjects) and patients with dengue infection (41 subjects). There were no significant differences in hemoglobin (Hb) and hematocrit (Hct) values between the groups, but differences were found in the number of leukocytes, absolute number of monocytes and platelet count (p<0.001, 0.002 and <0.001, respectively). The mean expression of FcγIIR monocytes in patients with dengue infection (208.77±32.06 median fluorescent intensity (MFI)) and the healthy subjects (124.03±47.76 MFI) with p<0.0001.CONCLUSION: The mean expression of FcγIIR monocytes in patients with dengue infection was higher than in healthy subjects.KEYWORDS: dengue infection, FcγII (CD32) receptor monocyte, flow cytometry
Normal Value of Thrombocytes Indices in Indonesian Adults: Focus on Gender and Ages Usi Sukorini; Adika Zhulhi Arjana; Tri Ratnaningsih; Rahmat Dani Satria
The Indonesian Biomedical Journal Vol 16, No 4 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i4.3205

Abstract

BACKGROUND: Monitoring platelet count and related indices is crucial for managing hematological disorders. No studies are reporting normal platelet indices in the adult Indonesian population. Therefore, this study was conducted to establish normal reference values for platelet count and indices in a healthy population in Indonesia and investigate their association with platelet count and each parameter.METHODS: This cross-sectional study included healthy adults from both sexes who underwent hematological testing in our laboratory. Two mL of venous blood was drawn and analyzed using an automated machine ADVIA 2120 to identify platelet count, mean platelet volume (MPV), platelet distribution width (PDW), plateletcrit (PCT), mean platelet component (MPC), platelet component distribution width (PCDW), mean platelet mass (MPM), platelet mass distribution width (PMDW), and large platelet count.RESULTS: This study included 1037 subjects with median of 27 (18–58) years old. Normal reference range values for platelet indices in adult Indonesians was established, which was significantly different between males and females (p<0.05) in following parameters: platelet count (191.77–400.37 vs. 203.00–433.00 ×109/L), MPV (7.30–9.91 vs. 7.20–10.00 fL), PDW (38.09–59.83 vs. 37.10–58.50%), PCT (0.17–0.33 vs. 0.17–0.35%), PMDW (0.68–1.06 vs. 0.67–1.04 pg) for males vs. females, respectively. However, other parameters such as MPC, PCDW, MPM, and large platelet, did not show any significant differences.CONCLUSION: The reference intervals of platelet indices in the adult Indonesian population were different from previously established reference values, indicating the importance of dedicated reference interval determination with gender consideration.KEYWORDS: thrombocyte indices, platelet count, hematology reference values, blood platelet analysis 
Co-Authors . Setyawati Adang Muhammad Gugun Adang Muhammad Gugun, Adang Muhammad Adika Zhulhi Arjana Aflah, Naja F Agus Supartoto Aiman, Sarah A Anak Agung Wiradewi Lestari Andri Christianalusi Andriani Tri Susilowati Angela Nurini Agni Anggraini Iriani Anik Ika Winarni Annisa Ginar Indrarsi Arlan Prabowo Budi Mulyono Diannisa Ikarumi Erlina Erlina Fatina, Marsya Kaila Fenty Fenty Fridawati, Veronica Gemilang, Rizka K Handoyo Pramusinto Hans Vrielink Harina Salim I Nyoman G Sudana Indrarsi, Annisa Ginar Ira Puspitawati Isti Setijorini Wulandari Kaslam, Surawijaya Bakhtiar Linda Rosita Luz Maria GBW Muhammad Juffrie Mulyono, Budi Natalia Christina Angsana Ni Kadek Mulyantari Ni Luh Putu Widhyasti Novenanto, Tandean Tommy Osman Sianipar Pratiwi Herowati Purbosari Purbosari Rahmat Dani Satria Riat El Khair Riswan Hadi Kusuma Rizki A. Gumilang Rizto Wisuda Senuari Rosmeri Handayani Rukmono Siswishanto Setyawati Setyawati Setyawati Setyawati Shanti Ika Sari Sianny Herawati Siti Muchayat Purnamaningsih Sri Mulatsih Suhardjo Prawiroranu Supomo Surawijaya Bakhtiar Kaslam Tandean Tommy Novenanto Tatang Talka Gani Teguh Triyono Teguh Triyono Teguh Triyono Telly Kamelia Tri Ratnaningsih Tri Ratnaningsih Tri Wahyu Widayanti Umi S. Intansari Veronica Fridawati Windarwati Windarwati