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All Journal Buletin Veteriner Udayana Jurnal Veteriner Indonesia Medicus Veterinus Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati ARSHI Veterinary Letters Jurnal Kedokteran Hewan

I Nyoman Sulabda

Laboratorium Fisiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jln Sudirman, Denpasar, Bali, Indonesia 80234

Author-ID : 390686

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Uji Alergi Ekstrak Akar Tuba Terhadap Kulit Anak Kucing Lokal Siswanto Siswanto; I Nyoman Sulabda; I Gede Soma
Buletin Veteriner Udayana Vol. 8 No. 2 Agustus 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Tuba root (Derris elliptica) contains a toxin rotenone which is an isoflavone compounds (flavonoids) included in the group of phenolic compound, which can kill fish and insects, including ticks and mites. Tuba root can be used as antiektoparasit in animals (eg fleas in cats), however research on skin sensitization of kitten to tuba extract was less or even not exist. This research used experimental animals; 6 kittens aged 1-3 months (unnoticed sexes) were divided randomly into two groups, three as a control (K) and three as treatment (P). Cats shorn size of 0.5 cm x 0.5 cm in the nape, abdominal and groin, then drops (one drop) the tuba root extract concentration of 15%, 20% and 25%. After 30, 60, and 120 minutes, visible signs that the onset of allergic skin itchy, red, or inflamed. The design used was completely randomized analysis method uses a variant level of 5%. Further data tested by LSD 5%. Results showed that the tuba root extract concentration of 15%, 20% or 25% did not cause an allergic on kitten skin (not significant p>0.05). It concluded that the tuba root extract is safe and can be used for anti-parasite in kittens aged 1-3 months.

PERCENTAGE OF VIABLE SPERMATOZOA COLLECTED FROM THE EPIDIDYMES OF DEATH LOCAL DOG I Nyoman Sulabda; I Ketut Puja
Buletin Veteriner Udayana Vol. 1 No. 2 Agustus 2009
Publisher : The Faculty of Veterinary Medicine, Udayana University

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The purpose of this study to determine the effectof post mortem time on percentage of lifeepididymessperm from postmortem dog caudae epididymides. A total of 9 dog were usedand divided into three group. T0 was control group, T1, 3 hours postmortem and T2, 6hours postmortem. This way, samples were obtained at different times postmortem. Spermwere extracted from the caudae epididymes by means of cuts.The result showed that the percentage of life sperm were 67,16 ± 5.67(T0), 46.33 ± 5.60(T1) and 24.00 ± 4.35 respectively. We could appreciate that percentage of life wasaffected by postmortem time. There was significant decrease life sperm recovered fromepididymes postmortem (P<0.01). In conclusion, epididymes sperm from dog undergodecrease of percentage of life, but it could stay acceptable within many hours postmortem.We intepreted these data to indicate that it may still be possible to obtain viablespermatozoa many hours later.

PENGARUH SUBSTITUSI AIR KELAPA MUDA DENGAN PENGENCER SITRAT KUNING TELUR TERHADAP MOTILITAS DAN PERSENTASE HIDUP SPERMATOZOA ANJING I Nyoman Sulabda; I Ketut Puja
Buletin Veteriner Udayana Vol. 2 No. 2 Agustus 2010
Publisher : The Faculty of Veterinary Medicine, Udayana University

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The purpose of this study was to determine the effect of tender coconut water substitutionon egg yolk citrate diluent with different doses on local breeds dog sperm motility and livespermatozoa. Semen was manually collected. Progressive motility and percentage of livespermatozoa were evaluated under a microscope utilizing a drop of semen between awarmed glass slide and coverslip, both at a temperature of 38 0C. The percentage of motileand live spermatozoa were examined by counting 100 spermatozoa using the classificationof Christiansen (1984). Sperm viability was assessed by eosinnegrosin staining. The result showed that coconut water substitution has significant effect on the motility and livespermatozoa . Combination between the levels of coconut water in the egg yolk citratediluent could be applied as an alternative diligent instead of egg yolk diligent for dogsemen up to 75%.

Residu Antibiotik Tetrasiklin dan Penisilin dalam Daging Sapi Bali yang Diperdagangkan di Beberapa Pasar di Bali (TETRACYCLINE AND PENILILLIN ANTIBIOTIC RESIDUES IN BALI BEEF WHICH ARE TRADED IN SEVERAL MARKETS IN BALI) Siswanto Siswanto; I Nyoman Sulabda
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Animal products that are marketed are found to contain antibiotics (residues), so that if consumed by the humans for a long time will have a negative effect on human health, therefore regular monitoring is needed. Therefore it is very important to do the research on antibiotic residues in beef in several market areas in Bali. This study aims to determine residual tetracycline and penicillin antibiotics on beef bali cattle in several markets in Bali-Indonesia. Research using 60 samples of beef originated from 5 different markets in Bali. As many as 12 sempels (100 g for each sample) was taken from each market that were purchased from different merchants. Screening tests (bioassay test) used to determine antibiotic residues in meat. The results showed that only three samples were positive containing antibiotics there were tertrasiklin 2 samples (3.33%) and penicillin1sample(1.67%). It was concluded that there were still antibiotic residues in beef marketed in Bali.

Kerapuhan Sel Darah Merah Sapi Bali (THE FRAGILITY OF ERYTHROCYTES OF BALI CATTLE) Siswanto .; I Nyoman Sulabda; I Gede Soma
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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An experimental study was conducted in order to evaluate the of erythrocyte on bali cattle atVeterinary Physiology Laboratory, Faculty of Veterinary Medicine, Udayana University, Denpasar, duringone month. Fifty blood samples were taken from bali cattle which slaughtered at Pesanggaran abattoir,Denpasar. The erythrocyte fragility was determined by using method Red Cell Fragility Procedure (2001).Results showed that the fragility (initial haemolysis) on red blood cell of bali cattle was observed, atranged between 0.45% - 0.55% NaCl , while for total haemolysis range between 0.30 - 0,35 % NaCl.

Polimorfisme Genetik DNA Mikrosatellite GEN BoLA Lokus DRB3 pada Sapi Bali (Bos indicus) I Ketut Puja; I Nengah Wandia; Putu Suastika; I Nyoman Sulabda
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i2.116

Tujuan penelitian ini adalah untuk mendapatkan informasi dasar mengenai distribusi frekuensi lokus DRB3 gen BoLa (bovine lymphocyte antigen) pada sapi Bali. Untuk isolasi DNA digunakan sampel darah sapi Bali yang diambil dari populasi sapi Bali yang berasal dari Bali dan sapi Bali yang berasal dari Nusa Penida. Jumlah sampel untuk sapi Bali yang berasal dari Bali adalah 22 ekor dan sapi yang berasal dari Nusa Penida 21 ekor. Jumlah allel lokus DRB3 pada sapi Bali asal Bali adalah 7 dan 9 allel dari sapi Bali asal Nusa Penida. Rataan heterozigositas perlokus adalah 0,7967 pada sapi Bali asal Nusa Penida dan 0,7863 pada sapi Bali asal Bali. Nilai PIC lokus DRB3 pada sapi Bali asal Nusa Penida adalah 0,7417 dan 0,742 pada sapi Bali asal Bali. Dapat disimpulkan dari hasil penelitian ini adalah lokus DRB3 pada sapi Bali sangat polimorfik.

Karakteristik Mikrosatelit Gen BoLA dengan Penanda Primer RM 185 pada Sapi Bali (Bos indicus) di Nusa Penida Putu Suastika; I Ketut Puja; I Nengah Wandia; I Nyoman Sulabda
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 17, No 2 (2012): June 2012
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v17i2.131

Penelitian ini bertujuan menjelaskan distribusi frekuensi lokus gen bovine lymphocyte antigen (BoLA) memakai primer RM 185 pada sapi bali dari Nusa Penida. Sebanyak 21 ekor sapi bali yang berasal dari Nusa Penida diambil secara acak. Jumlah alel gen BoLA pada lokus RM 185 ada 7 macam alel yaitu 76 pb, 84 pb, 86 pb, 90 pb, 98 pb, 100 pb, dan 104 pb. Frekuensi tiap- tiap alel yang teramati adalah 0,02%; 0,09%; 0,31%; 0,07%; 0,12%; 0,28%; dan 0,09%. Rata- rata heterozigositas yang didapat adalah 0,804. Dapat disimpulkan, bahwa lokus RM 185 pada gen BoLA sapi bali di Nusa Penida adalah sangat polimorfik di antara populasi.

Karakterisasi Genetik Kambing Gembrong dari Karangasem Bali Menggunakan DNA Mikrosatelit I Ketut Puja; I Nyoman Sulabda
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i1.2632

The present study was undertaken with primary objective to characterize of Gembrong goat breeds. It is essential to characterize the germplasm for intragenetic variability, which will help in planning for conservation strategy as well as genetic improvement. DNA Genome was isolated from hairs. Nine microsatellite were amflified by PCR. PCR product were run on 6% bis-Acrylamide gel in automated DNA sequencer. Flourescent signals from the dye-labeled microsatellite were detecting using STRand software. The result show that all markers (INRA005, INRA063, INRA023, ILSTS19, ILSTS87, SRCRSP8, MAF65, OarFCB20, and McM547) successfully amplified in Gembrong goat microsatellite loci. The number of alleles per locus ranged from 1 (INRA005) to 4 (ILSTS87). All the microsatellites investigated were found to be highly polymorphic, except INRA063. In total, 23 alleles were observed for the 9 microsatellite loci. The allele sizes ranged from 99 bp (OarFCB20) to 240bp (SRCRSP8). The study can be extended to include large many microsatellites in different chromosome location to validate the results.

ASOSIASI POLIMORFISME GENETIKA LOKUS DEOXYNUCLEIC ACID (DNA) MIKROSATELIT GEN BOVINE LYMPHOCYTE ANTIGEN (BoLA) DENGAN KUALITAS SEMEN PADA SAPI BALI I Ketut Puja; I Nengah Wandia; Putu Suastika; I Nyoman Sulabda
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Tujuan penelitian ini adalah mendapatkan informasi dasar mengenai struktur genetika menggunakan marka melokuler DNA mikrosatelit gen bovine lymphocyte antigen (BoLA) dan hubungannya dengan kualitas semen sapi bali. Data diambil dari 8 ekor sapi jantan yang ada di Unit Pelaksana Teknis Daerah (UPTD) Inseminasi Buatan, Baturiti, Tabanan, Bali. Hasil pemeriksaan kualitas sperma menunjukkan bahwa volume semen sapi bali 3,0-6,5 ml, konsentrasi spermatozoa 800-11.000 juta sel/ml, dan persentase spermatozoa motil 70-75%. Hasil penelitian pada sapi bali menunjukkan bahwa ketiga lokus mikrosatelit yang digunakan teramplifikasi pada sapi bali dan jumlah alel pada lokus RM185 dan BM1815 adalah 2 sedangkan jumlah alel pada lokus DRB3 adalah 1. Heterozigositas per lokus berkisar 0-0,533. Nilai PIC per lokus antara 0-0,375. Dari hasil penelitian ini dapat disimpulkan bahwa ukuran alel pada ketiga mikrosatelit berpotensi sebagai marka molekuler yang berperan terhadap kualitas semen pada sapi bali.

Penyakit tetelo pada ayam lokal – Laporan kasus Hartaputera, I Nyoman Surya Tri; Kencana, Gusti Ayu Yuniati; Adi, Anak Agung Ayu Mira; Sudipa, Putu Henrywaesa; Sulabda, I Nyoman
ARSHI Veterinary Letters Vol. 8 No. 1 (2024): ARSHI Veterinary Letters - February 2024
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.8.1.9-10

Peternakan ayam lokal tradisional, dibandingkan dengan peternakan unggas komersial, menghadapi banyak tantangan terkait penyakit, dengan salah satu ancaman utama adalah virus penyakit tetelo. Laporan kasus ini menggambarkan ayam lokal berumur 8 minggu yang terinfeksi virus Newcastle disease terdeteksi melalui pemeriksaan aspek epidemiologi, patologi, dan virologi. Angka kesakitan di peternakan ini adalah 65%, dengan angka kematian 20% dan angka kematian 30,7%. Hasil pemeriksaan anatomi dan histopatologi menunjukkan adanya perubahan patologis pada berbagai organ. Uji laboratorium menggunakan uji Haemmaglutination-Inhibition dan Haemmaglutination memastikan adanya virus penyakit tetelo. Berdasarkan gejala klinis, temuan patologi anatomi, analisis histopatologi, isolasi virus, dan identifikasi, disimpulkan bahwa ayam yang terkena penyakit tersebut terinfeksi penyakit Newcastle velogenik viscerotropik.

Co-Authors Adi, Anak Agung Ayu Mira Agustina, I Putu Sudana Aminuyati Anak Agung Sagung Kendran Ardi Apriandi, Ardi Bendelin Melda Loekali Chandra, Michele Damara, Doni Ginting, Regina Bonifasia Br Gusti Ayu Yuniati Kencana Hartaputera, I Nyoman Surya Tri I Gede Soma I Gusti Ngurah Sudisma I Ketut Berata I Ketut Puja I Ketut Sumadi I Made Merdana i Nengah Wandia I Wayan Gorda Ida Ayu Pasti Apsari Ida Bagus Komang Ardana Iwan Harjono Utama Juniartini, Wieke Sri Listiana Dewi, Ni Luh Putu Yunita Luh Dewi Anggreni Manuela, Ni Luh MERRY NAOMI VITASARI DAMANIK Ni Luh Eka Setiasih Novanti, Ni Putu Gupta Pabala, Meiksilano Ferdy Paraningtyas, Alya Diasti Permana, Rizky Putra, I Dewa Agung Made Wihanjana Putu Henrywaesa Sudipa Putu Suastika Sis wanto Siswanto - Siswanto Suastika , Putu Suharsono, Hamong Utomo, Kurniawan Cahyo

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