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KEANEKARAGAMAN KHAMIR PENDEGRADASI MINYAK HASIL ISOLASI DARI PELABUHAN TANJUNG PERAK SURABAYA Tri Nurhariyati; Ni’matuzahroh Ni’matuzahroh; Tini Surtiningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 2 (2004): June 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/427

Abstract

The aims of this research was to obtain diversity of hydrocarbonoclastic yeast isolated from Tanjung perak harbor Surabaya. Exploration of yeast was conducted by isolation and identification of isolated yeast. Identification of yeast based on characteristics of colonies, cell shape, and biochemical tests. From this research, it was obtained 9 hydrocarbonoclastic yeasts. They were 8 generas: Rhodotorula, Candida, Geotrichum, Torulopsis, Trichosporon, Cryptococcus, Debaryomyces, and Saccharomyces.
BIOREMEDIASI Cd DAN KETERSEDIAAN P BATUAN FOSFAT OLEH CENDAWAN EKTOMIKORHIZA (Pisolithus tinctorius dan Suillus granulatus) DALAM KULTUR MURNI Tini Surtiningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 3 No 2 (1997): December 1997
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/511

Abstract

Some rock phosphates used as fertilizers or to produce fertilizers which containing very high cadmium and have been suspected to increase trace element content in soild. In order to know if the cadmium can be mobilized, we have studied the bio-remediation of Cd and bio-availibility of P from three rock phosphates (from North Caroline, Togo and Senegal) by ectomycorrhyzal fungi (Pisolithus tinctorius and Suillus granulatus) in pure culture. In pure culture the fungi accumulated 80-100% Cd soluble. In the experiments, the % bio-accumulation or bio-remediation of Cd was not always correlated with the Cd content of the phosphate nor their hardness.
Remediation of Pb(II) and Cd(II) in Sea Water by Skeletonema costatum Thin Soedarti; Tini Surtiningsih; Sucipto Hariyanto; Eko P. Kuncoro
Sustinere: Journal of Environment and Sustainability Vol. 1 No. 2 (2017): pp. 63 - 143 (December 2017)
Publisher : Center for Science and Technology, IAIN Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22515/sustinere.jes.v1i2.19

Abstract

The heavy metals that polluted sea water can end up polluting fish pond too. Hence, it is necessary to remove the heavy metals before sea water enters the fish pond. The aim of this study was to know the growth, remediation ability, and the highest of removal efficiency of marine diatom Skeletonema costatum in the lead (Pb(II)) and cadmium (Cd(II)) solution. This study used 3 x 4 factorial design, i.e. concentrations of the cell inoculation (5000 cells mL-1; 10000 cells mL-1; and 15000 cells mL-1) and concentrations of Pb(II) and Cd(II) (0; 0.5; 1; and 2 ppm), replicated five times. During five day exposure time, the cell density was observed daily using a microscope (400X), while filtrate of media was analyzed using AAS. The result showed that S. costatum could grow in Pb(II) and Cd(II) at 2 ppm, and could remediate Pb(II) and Cd(II) at 2 ppm. The highest removal efficiency of marine diatom S. costatum in Pb(II) solution was 80.5% (5000 cells mL-1 at 2 ppm on the first day), and in Cd(II) solution was 80% (15000 cells mL-1 at 0.5 ppm on the fifth day).
Pycnoscelus surinamensis Endosymbiont Bacteria: Identification and its Amylase Characteristic Ni'matuzahroh; Fatimah; Sholikah; Ayu Tri Wahyuni; Tri Nurhariyati; Mochammad Affandi; Agus Supriyanto; Almando Geraldi; Tini Surtiningsih; Brigita Nur Diyan Agustiana; Dela Dwi Alawiyah; Ana Mariatul Khiftiyah; Silvia Kurnia Sari
Journal of Bio-Molecule Research and Engineering Vol 2 No 2 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i2.67571

Abstract

The amylase is an enzyme that widely used in industry. In this research, amylase was isolated from endosymbiont bacteria (EKA 4) of cockroach Pycnoscelus surinamensis from household garbage dump Surabaya. This research aims to identify bacteria EKA 4, produce and characterize amylase from it at various temperature, pH and effect of metal ions. Amylase screening assay was done by imoculating the isolate into medium contain 2% of starch then incubated for 24 hours. Detection of amylase was done qualitatively by measured presence of halozone and quantitatively using DNS method. 16S rRNA analysis found that bacteria EKA 4 has similarity to Bacillus subtilis with 100% of percent identity. Result showed that optimum activity of amylase from B. subtilis were at 40°C, pH 6 and could use ion CaCl2 as a cofactor in a reaction. Thus, utilization of amylase from endosymbiont P. surinamensis could be as an alternative source in big industries.
Pengaruh Pemberian Mikoriza Glomus sp., Trichoderma harzianum dan Pupuk Hayati Terhadap Pertumbuhan dan Produksi Tanaman Jagung (Zea mays L.) Tiara Primayanti; Tini Surtiningsih; Thin Soedarti; Sucipto Hariyanto
Biotropic : The Journal of Tropical Biology Vol. 9 No. 1 (2025): Biotropic, Volume 9 Nomor 1, 2025
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v9i1.2267

Abstract

The aim of this study was to evaluate the impact of mycorrhizal applications of Glomus sp., Trichoderma harzianum species, and organic biofertilizers on the growth parameters and productivity of corn (Zea mays L.). This experiment was conducted in the period of May-July 2012 on agricultural land located in Kendal Village, Kendal District, Ngawi Regency. The applied methodology was a completely randomized design (CRD) with four treatment groups; (1) the control group, (2) the Glomus sp. mycorrhiza group as much as 30 g/plant, (3) the T. harzianum group as much as 30 ml/plant, (4) the biological fertilizer group as much as 5 ml/plant with each treatment repeated five times. Data related to plant growth and production were analyzed using the Analysis of Variance (ANOVA) test, with the Duncan test at a significance 5% to determine significant differences between treatments.. The results from the four treatments show that the application of Glomus sp., T. harzianum, and organic biofertilizers have a significant effect (α < 0.05) on the growth and yield of corn. T. harzianum treatment produced the highest plant height, which was 181 ± 8.9 cm, while the application of Glomus sp. mycorrhiza gave the best contribution to root length parameters with an average of 41.2 ± 5.3 cm. The application of biofertilizer also provides an optimum result for corn weight of 144 ± 28.9 g