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Journal : Berita Kedokteran Masyarakat

Bacterial contamination of tomatoes sellers in eight traditional markets of Jember Regency, East Java, Indonesia Laili, Nikmatul; Suswati, Enny; Abrori, Cholis; Mufida, Diana Chusna; Hermansyah, Bagus; Putri, Elvia Rahmi Marga
BKM Public Health and Community Medicine Vol 40 No 04 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bkm.v40i04.9307

Abstract

Purpose: Foodborne diseases are a significant threat to public health and is often associated with pathogenic microorganisms entering the body due to consuming contaminated food. One of the factors causing foodborne diseases was the contamination of pathogenic bacteria in vegetables consumed raw, one of which is tomatoes. This study was conducted to determine the correlation between traders' personal hygiene behavior and bacterial contamination of tomatoes in the traditional Jember Regency market. Methods: This study used analytical observational research with a cross-sectional research design, conducted in the Laboratory of Microbiology, Faculty of Medicine, University of Jember. The large sample size of this study was 40 traders. Personal hygiene behavior of traders was obtained from direct observation with observation sheets in eight selected traditional markets in Jember Regency. Results: Tomato samples were contaminated with bacteria with the highest bacterial colony >2.5 x 108 CFU/g and the lowest 4.6 x 105 CFU/g. The types of bacteria found in this study were Vibrio parahaemolyticus (15%), Shigella sp. (62.5%), Salmonella sp. (72.5%), Vibrio cholerae (82.5%), Escherichia coli (90%), and Staphylococcus aureus (92.5%). The bivariate test results showed no significant correlation between personal hygiene, sanitation, supporting facilities, and traders' characteristics with bacterial contamination of tomatoes sold in eight Jember traditional markets. Conclusion: This study concludes that there is no significant correlation between traders' personal hygiene behavior and bacterial contamination in tomatoes sold in eight traditional markets of Jember Regency. Consumers should wash tomatoes well or process them first before consumption.
Correlation of urban and rural settlements with Shigella sp. resistance pattern isolated from Bedadung River in Jember, Indonesia Aryasatya, Dimas Widyadhana Bhanu; Agustina, Dini; Jauhani, Muhammad Afiful; Suswati, Enny
BKM Public Health and Community Medicine Vol 41 No 01 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bkm.v41i01.13344

Abstract

Purpose: This study aims to determine a correlation between settlement location and resistance patterns of Shigella sp. isolated from the Bedadung River in Jember, Indonesia. Method: The research method used was analytic observational with a cross-sectional design. Data analysis was performed using the Chi-Square test and, if not qualified, the Fisher exact test. Samples were obtained through a total sampling technique with a total sample size of 24. Results: Most samples were resistant to the antibiotic, and there was no relationship between residential location and resistance patterns of Shigella sp. isolated from Bedadung River, Jember Regency (p-value > 0.05). Conclusions: In conclusion, no significant relationship exists between the residential area and the resistance pattern of Shigella sp. isolated from Bedadung River, Jember Regency. Shigella sp. bacterial contamination is mainly found in Bedadung River water isolates in rural and urban areas. Shigella sp. bacteria found in rural and urban areas in the Bedadung River in Jember Regency have the same number.
The Relationship between Milking Hygiene and Salmonella sp. Contamination on Cow’s Milk in Jember Regency Mufida, Diana Chusna; Shabri, Hammam Arif; Hermansyah, Bagus; Shodikin, M Ali; Suswati, Enny
BKM Public Health and Community Medicine The 12th UGM Public Health Symposium
Publisher : Universitas Gadjah Mada

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Abstract

Purpose: Milk is a liquid produced by the mammary glands of female mammals. The nutritional content of milk almost covers the entire nutritional needs required by living things. The appearance of Salmonella sp. in cow's milk is due to milking methods that do not meet the standards, especially regarding milking hygiene management. This study examines the relationship between milking hygiene and Salmonella sp. contamination in cow's milk in Jember Regency. Method: This study used observational analytic research with a cross-sectional research design. Primary data were obtained through direct observation of milkers using a checklist related to milking. observe Salmonella sp. contamination using culture techniques on SCA (Salmonella Chromogenic Agar) as selective media agar for Salmonella sp. and Gram staining. Results: The results from this study show that the hygiene process pre-milking (78.9%), during-milking (71.1%), and post-milking (89.5%) have been implemented. The statistical test results showed a relationship between the pre-milking hygiene process with Salmonella sp. contamination (p-value < 0.05), but insignificant results were seen in the during and post-milking hygiene processes (p-value> 0.05). Conclusion: The conclusion from this study show that the three results only the hygiene processes pre-milking have results associated with Salmonella sp. contamination in cows' milk. Therefore, milkers are expected to maintain and improve dairy hygiene measures, especially before milking to prevent contamination of cow's milk.
High rate of multidrug-resistant Escherichia coli isolated from ready to eat: crispy fried chicken in Jember: a real threat to human health Suswati, Enny; Supangat
BKM Public Health and Community Medicine The 12th UGM Public Health Symposium
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Purpose: This study was conducted to assess prevalence and antibiotic resistance profile of E. coli from ready-to-eat crispy fried chicken (CFC) in Jember city, Indonesia. Methods: This cross-sectional study was conducted from November 2020 to April 2021 by collecting a total of 81 CFC samples from 27 food stalls in campus area using a simple random sampling method. Isolation and determination of E. coli use were performed by conventional culture method and an antibiotic susceptibility test was conducted by using Kirby Bauer disk diffusion method on the Mueller–Hinton agar. Result: Out of 81 CFC samples, 77 (95.06%) were positive for E. coli. High E. coli drug resistance was observed on ampicillin, amoxicillin, (100%) followed by cefixime (98.72%), erythromycin (97.59%), sulfamethoxazole (93.59%), azithromycin (83.33%), cefotaxime (78.28%), chloramphenicol (75.64%), and cefixime (74.36%). On the other hand, there was the highest susceptibility for ciprofloxacin (64.10%). Multidrug resistance was also found in 76/77 (98.70%) of the isolates. MAR index was 0.4-1. Conclusion: The resistance pattern CfmCroAmlAmpAzmCtxSxtCE was the most common among the E. coli isolates, with 17 showing it. The multiple antibiotic index (MAR index) ranged from 0.4 to 1. Hygienic measures should be rigorously implemented, and monitoring resistance of E. coli is required to reduce the risks related to the emergence of multi-resistant bacteria.
Co-Authors Abu Khoiri Aditya Oktavianto Ahmad Hasbi Al-Muzaky Ahmad Hasbi Al-Muzaky Alfianti, Nita Angga Mardro Raharjo Anggraini, Nanda Dewi Anisa Rizca Putri APRI KUNTARININGSIH Arifah Nur Hasanah Ariska Nur Aida Aryasatya, Dimas Widyadhana Bhanu asihanti rosita ferdiana Azham Purwandhono Bagus Hermansyah Bagus Hermansyah Bagus Satrio Pambudi Bagus Satrio Pambudi, Bagus Satrio Cholis Abrori, Cholis Desie D. Wisudanti Desie Dwi Wisudanti Devi Chintya Kumalasari Dian H. Purnamasari Diana C Diana C. Mufida Diana Chusna Mufida Dini Agustina Dwita Aryadina Dwita Aryadina Rachmawati Elly Nurus Sakinah, Elly Nurus Elvia Rahmi Marga Putri Erfan Efendi Habib Mustofa Haq, Hanifah S Ika Rahmawati Sutejo Indra Samudra Rahmat Irawan Fajar Kusuma Jauhani, Muhammad Afiful Jauhar Firdaus Joko Mariyono Kirana Nadyatara Kristianningrum Dian Sofiana Lailatis Shofia Laili, Nikmatul Laksmi Indreswari Lutfadaturroifa, Alya Wafaul M. Ali Shodikin Misnawi Misnawi Misnawi Misnawi Mufida Mufida Mufida, Diana C Muhammad A. Shodikin Muhammad Alif Taryafi Mukarromah, Laylatul Fitriyah Nadhiva, Alvien Zahrotun Nexia Nevarachell Onny Amirsyah Nisrina Salsabila Firmansyah Nita Alfianti Nugraha, Bima Setia Sandya NUR K. AGUSTIN Nurhayati Nurhayati Pratama, Dava Rizky Prayoga Adinawer Sirait Probosutiksna, Nuno Febrian Probosutiksna, Nuno Febrian Putra Uta, Diego Rafi Putri, Elvia Rahmi Marga Putu Ayu Laksmi Lestari Raharjo, Angga M Rahmat, Indra Samudra Regina Finka Risty Pradana Linggan Wangi Rosdiana, Fetri Rumastika, Nindya Shinta S.Pd. M Kes I Ketut Sudiana . Sakinah, Elly N Salsabila, Yuna Annisa Saskia Mediawati septa surya wahyudi, septa surya Shabri, Hammam Arif Shodikin, M Ali Shodikin, M. Ali Shofia, Lailatis Sudarmanto, Yohanes Supangat Supangat Supangat Supangat Supangat Suryono Suryono Syahputri, Siti Ananda Hardita Ulfa Elfiah Wahyuning Ati Ashari Wiwien Sugih Utami Wiwin Sugih Utami Yudha Nurdian Yudha Nurdian Yuna Annisa Salsabila Yunita Armiyanti Yunita Wulansari