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All Journal Medical Journal of Indonesia Narra J Makara Journal of Health Research Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Din Syafruddin
Eijkman Institute for Molecular Biology
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Malaria and Anopheles spp in the villages of Salubarana and Kadaila, Mamuju District, South Sulawesi Province, Indonesia Nurdin, Armyn; Syafruddin, Din; Wahid, Isra; Noor, Nur N.; Sunahara, Toshihiko; Mogi, Motoyoshi
Medical Journal of Indonesia Vol 12, No 4 (2003): October-December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.954 KB) | DOI: 10.13181/mji.v12i4.119

Abstract

Malaria epidemiology in Salubarana and Kadaila villages, Mamuju District, South Sulawesi Province, Indonesia was studied from July-December 2002 to obtain baseline data related to the parasite, mosquito vector, human host, and environmental factors. Monthly malariometric surveys conducted during the six- month period revealed a relatively high prevalence of malaria in Kadaila in comparison to Salubarana. Kadaila was mainly inhabited by migrants from Java, Bali, Lombok, and from other districts of South Sulawesi. Salubarana, on the other hand, was inhabited mainly by indigenous Mandarese. Analysis of 1,113 blood smears taken from individuals randomly involved in the survey revealed 59 positive samples, consisting of Plasmodium falciparum or P. vivax. These two species predominated the samples examined and were occasionally found as mixed infection. Anopheles barbirostris was confirmed as a vector for malaria in this area whereas 7 other species An. barbumbrosus, An. parangensis, An. vagus, An. crawfordi, An.pseudobarbirostris, An. Tessellatus, and An. subpictus should be considered as potential vectors. The first mentioned species predominated, using mainly riverbeds and ground pools as breeding places with a habit of indoor or outdoor resting. These findings could be useful for establishing evidence-based malaria control program in the area. (Med J Indones 2003; 12: 252-8) Keywords: malaria epidemiology, parasite, vectors, environmental factors, Indonesia
Malaria in Jayapura District, Papua Province, Indonesia and resistance to sulfadoxine-pyrimethamine Sorontou, Yohanna; Asih, Puji B.S.; Wanandi, Septelia I.; Ramelan, Wahyuning; Syafruddin, Din
Medical Journal of Indonesia Vol 16, No 1 (2007): January-March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.135 KB) | DOI: 10.13181/mji.v16i1.254

Abstract

Malaria poses a major public health problem in Papua. The rapid spread of drug-resistant parasites and deterioration of the malaria control program following the monetary crisis and civil strife in the late 90’es have worsened the malaria situation in many areas of Papua. To re-assess the current magnitude of malaria problem, a malariometric survey was conducted in three sub-districts of Jayapura District, as well as antimalarial drug sensitivity test using sulfadoxine-pyrimethamine (SP) at the outpatient clinics of Abepura General Hospital (RSU), Hamadi Primary Health Center and Panti Asuhan Katolik Clinics, Sentani, Jayapura District, Papua Province. Screening of 543 subjects in three sub-districts revealed 185 malaria positive subjects, either with P. falciparum, P. vivax or mixed of the two species. All of the malaria cases were asymptomatic. In the SP drug sensitivity test, of the 56 subjects involved, 32 were classified as early treatment failure and two subjects were classified as late treatment failure. The remaining 22 subjects were classified as adequate clinical and parasitological response (ACPR). These results indicated that the surveyed area has a high malaria prevalence and treatment failure to SP and therefore alert to the need of deploying new antimalarial regiment that suit the remote setting. (Med J Indones 2007; 16:32-8) Keywords: malaria, sulfadoxine-pyrimethamine (SP) resistance, Jayapura District, Indonesia
Challenges that Hinder Parturients to Deliver in Health Facilities: A Qualitative Analysis in Two Districts of Indonesia Nasir, Sudirman; Ahmed, Rukhsana; Kurniasari, Miladi; Limato, Ralalicia; Tulloch, Olivia; Syafruddin, Din; Koning, Korrie De
Makara Journal of Health Research Vol. 20, No. 3
Publisher : UI Scholars Hub

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Abstract

Background: There are many challenges women face to be able to give birth in health facilities in many parts of Indonesia. This study explores the roles and observations of close-to-community maternal health providers and other community members on potential barriers faced by women to deliver in health facilities in two districts within The Archipelago. Methods: Employing an explorative qualitative approach, 110 semi-structured interviews and 7 focus group discussions were conducted in 8 villages in Southwest Sumba, in the East Nusa Tenggara province, and in 8 villages in Cianjur, in the West Java province. The participants included village midwives, Posyandu volunteer (village health volunteers), traditional birth attendants (TBAs), mothers, men, village heads and district health officials. Results: The main findings were mostly similar in the two study areas. However, there were some key differences. Preference for TBA care, traditional beliefs, a lack of responsiveness of health providers to local traditions, distance, cost of travel and indirect costs of accompanying family members were all barriers to patients attending health facilities for the birth of their child. TBAs were the preferred health providers in most cases due to their close proximity at the time of childbirth and their adherence to traditional practices during pregnancy and delivery. Conclusions: Improving collaborations between midwives and TBAs, collaboration, and responsiveness to traditional practices within health facilities and effective health promotion campaigns about the benefits of giving birth in health facilities may increase the use of health facilities in both study areas.
Evaluation of an E. coli-expressed spike protein-based in-house ELISA system for assessment of antibody responses after COVID-19 infection and vaccination Nurisyah, Sitti; Iyori, Mitsuhiro; Hasyim, Ammar A.; Amru, Khaeriah; Itani, Kei; Nakamura, Kurumi; Zainal, Kartika H.; Halik, Handayani; Djaharuddin, Irawaty; Bukhari, Agussalim; Asih, Puji BS.; Syafruddin, Din; Yoshida, Shigeto; Idris, Irfan; Yusuf, Yenni
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1250

Abstract

Evaluating long-term immunity after COVID-19 infection and vaccination is critical for managing potential outbreaks. The aim of this study was to develop a cost-effective in-house enzyme-linked immunosorbent assay (ELISA) based on Escherichia coli-expressed SARS-CoV-2 spike protein (E-S1) for antibody detection and to evaluate its performance. The system was validated by comparing the in-house ELISA results with those obtained using a commercial ELISA with HEK293-expressed spike protein (H-S1). Recombinant SARS-CoV-2 spike protein was produced in E. coli, purified, and validated for antigenicity via ELISA. Indirect ELISAs with both E-S1 and H-S1 antigens were performed on 386 serum samples from COVID-19 survivors, vaccinated individuals, and pre-pandemic controls collected at different time points. The E-S1 ELISA showed a statistically significant but weak correlation with H-S1 ELISA across all samples (r=0.205; p=0.0001). Stronger correlations were observed among vaccinated individuals with prior infection on day 90 (r=0.6017; p<0.001) and in naïve vaccine recipients on day 30 (r=0.5361; p=0.0003). Pre-pandemic sera from a rural population in Sumba Island exhibited high background reactivity in E-S1 ELISA, likely due to anti-E. coli antibodies, while urban pre-pandemic sera from Jakarta showed a stronger correlation with H-S1 ELISA. This suggests potential regional or immune background differences influencing assay performance. Although E-S1 retained antigenic properties, its diagnostic utility is limited by non-specific reactivity and reduced sensitivity compared to H-S1. In conclusion, E. coli expression systems may not be ideal for producing spike protein-based ELISA antigens specific to SARS-CoV-2. Alternative expression systems, such as human or baculovirus, could enhance diagnostic accuracy and specificity for COVID-19 antibody detection.
Aminolevulinic Acid Dehydratase Allelic Frequency and Lead Toxicity in Children Under-Five in a Former Used Lead-Acid Battery Area Irawati, Yana; Kusnoputranto, Haryoto; Achmadi, Umar Fahmi; Safrudin, Ahmad; Sitorus, Alfred; Risandi, Rifqi; Wangsamuda, Suradi; Permana, Dendi Hadi; Syahrani, Lepa; Dewayanti, Farahana Kresno; Asih, Puji B S; Syafruddin, Din
Kesmas Vol. 17, No. 1
Publisher : UI Scholars Hub

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Abstract

Polymorphisms in the Aminolevulinic Acid Dehydratase (ALAD) gene responsible for the ALAD1 and ALAD2 alleles have been implicated in susceptibility to lead toxicity. This study aimed to determine the allelic frequency of ALAD2 among children living in Bogor District, Indonesia, and its association with blood lead levels (BLLs) and lead toxicity. A cross-sectional study involving 128 children was conducted during September-October 2019 in the former ULAB area in Cinangka Village. The ALAD polymorphism, BLLs, and hematological parameters were evaluated. Blood samples were taken for dried blood spotting on filter paper, blood film, and BLL measurement. The PCR amplification and sequencing of the genomic DNA revealed the presence of two forms of the ALAD2 allele: 177C and 177T with a frequency of 0.05. Analysis of the correlation between the ALAD2 allele, BLLs, and basophilic stippling revealed that ALAD2 carriers had a five times higher risk of high BLLs, (OR = 5.359, p-value = 0.155) and had a slightly higher risk of exhibiting basophilic stippling (OR = 1.09, p-value = 1.000). Although not statistically significant, these findings suggested that the ALAD genotype may modify BLLs and lead to toxicity. The ALAD2 allele(177T) is firstly reported in any population in the world.
Co-Authors Ahmad Safrudin Ahmed, Rukhsana Amru, Khaeriah Armyn Nurdin Asih, Puji B S Asih, Puji BS. Bukhari, Agussalim Dewayanti, Farahana Kresno Djaharuddin, Irawaty Halik, Handayani Haryoto Kusnoputranto Hasyim, Ammar A. Irawati, Yana IRFAN IDRIS Isra Wahid Itani, Kei Iyori, Mitsuhiro Koning, Korrie De Limato, Ralalicia Miladi Kurniasari Motoyoshi Mogi Nakamura, Kurumi Nur N. Noor Nurisyah, Sitti Permana, Dendi Hadi Puji B.S. Asih Risandi, Rifqi Septelia I. Wanandi Sitorus, Alfred Sudirman Nasir Syahrani, Lepa Toshihiko Sunahara Tulloch, Olivia Umar Fahmi Achmadi Wahyuning Ramelan Wangsamuda, Suradi Yohanna Sorontou Yoshida, Shigeto Yusuf Yenni Zainal, Kartika H.