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All Journal Jurnal Teknologi Dan Industri Pangan Biospecies Jurnal Penelitian Pendidikan IPA (JPPIPA) Idaarah: Jurnal Manajemen Pendidikan Bubungan Tinggi: Jurnal Pengabdian Masyarakat Jurnal Biosense BiosciED: Journal of Biological Science and Education Proceeding Biology Education Conference BIOMA : Jurnal Biologi dan Pembelajarannya Jurnal Ilmiah Ilmu Terapan Universitas Jambi BIO PALEMBANICA
Titin Purnaningsih
Program Studi Pendidikan Biologi, FKIP, Universitas Palangka Raya
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Engineering Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Other

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Journal : Biospecies

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Analysis of Somaclonal Variation in Cinchona succirubra Using Random Amplified Polymorphic DNA (RAPD) Titin Purnaningsih
Biospecies Vol. 6 No. 1 (2013): Januari 2013
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v6i1.683

Abstract

Regenerants of C. succirubra were examined by RAPD analysis to determine theoccurrence and extent of somaclonal variation. Plantlets were regenerated by nodal culture. DNAwas extracted from the parent plant and regenerants of C. succirubra. Fifteen base syntheticoligonucleotides from Operon were chosen that gave multiple polymerase chain reaction (PCR)amplification products using cinchona DNA from parent plants grown in the field and plantlets after 4and 5 passages or 4 and 5 month old in vitro culture. The results showed that all of morphologicalcharacters on regenerated plants were clonally uniform and all of the RAPD profiles were generallymonomorphic, stable, and similar to parent plants. These results demonstrated that RAPD can beused successfully to determine the somaclonal variation among regenerated plants.
Co-Authors Adam, Chaidir AGUS HARYONO Akhmadi Akhmadi Akhmadi Anggoro, Rahayu Opi Anton Rahmadi Araina, Elga Decenly Decenly Decenly Edy Prasetyo Ennike Gusti Rahmi Fahrina, Ririn Fatiqin, Awalul Gunawan, Yohannes Edi Hartanti, Rio Eka Desi Purwandari Hasanah, Rizka Ipnatul Abdiah Irena Stephani Ambarita Karina Evaying Kimeni Kusuma Putri, Rahmadyah Liswara Neneng Maria Elisha Otis Mashabhi, Sugeng Maya Dewi Sukarno Miranda, Yula Miranda, Yula Nuriman Otis, Maria Elisha Rahayu Opi Anggoro Rahmadyah Kusuma Putri Rahmadyah Kusuma Putri Rahmawati, Rahmawati Riky Riky Rizka Hasanah Silvita Siti Sunariyati Sri Puryaningsih Sugeng Mashabhi Syafutra, Randy Wijaya, Nuriman Yessy Velina