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All Journal HAYATI Journal of Biosciences Jurnal Bios Logos EUGENIA Jurnal Ilmu dan Teknologi Pangan Pro Food Jurnal Entomologi Indonesia Jurnal Agroekoteknologi Terapan AGRI-SOSIOEKONOMI COCOS
Dantje Tarore
Pascasarjana Universitas Sam Ratulangi Program Studi Entomologi Minat Entomologi Kesehatan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences

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Integrated DNA Barcoding and Morphometric Characterization of Palm Weevils (Rhynchophorus spp.) in North Sulawesi Balansa, Endrile Golmen; Salaki, Christina Leta; Tarore, Dantje; Mamahit, Juliet Merry Eva; Kolondam, Beivy Jonathan; Tallei, Trina Ekawati
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1260-1272

Abstract

Palm weevils (Rhynchophorus spp.) are significant pests of sago palms worldwide. Yet, the taxonomy and evolutionary lineage of these species in North Sulawesi remain unclear, likely due to geographic isolation driving genetic variation and species differentiation. This study aimed to investigate the genetic diversity and morphological differentiation of Rhynchophorus across distinct geographic regions in North Sulawesi using an integrative approach combining DNA barcoding and morphometric analysis. Morphometric traits from palm weevil specimens collected in Sangihe Island, Minahasa, and Bolaang Mongondow were measured and statistically analyzed using one-way ANOVA, MANOVA, principal component analysis (PCA), and linear discriminant analysis (LDA) to assess interpopulation morphological differences. For molecular identification, the cytochrome oxidase I (COI) gene was amplified and sequenced. Phylogenetic relationships were inferred using the maximum likelihood method, and a DNA barcode gap analysis was conducted to evaluate the separation between intra- and interspecific genetic distances. Morphometric analysis revealed significant size variations among the specimens, particularly in rostrum dimensions, with the EBBM (Bolaang Mongondow) group showing the highest values. COI-gene-based identification confirmed that all specimens were of R. vulneratus. However, phylogenetic analysis showed EBMin (Minahasa) and EBBM forming a distinct subgroup, while EBMan, EBSTS, EBTam, and EBSTU (all from Sangihe Island) clustered separately. Barcode gap analysis demonstrated a clear distinction between intra- and interspecific divergence, validating COI as a reliable marker for species delimitation. This study concludes that integrating morphometric and genetic analyses reveals geographic structuring within R. vulneratus, highlighting the effectiveness of combined methods for accurate identification and population differentiation.
Entomopathogenic Fungi Efficacy Test Beauveria bassiana (Bals) Vuill on Spodoptera exigua (Hubner) Larvae Mortality in Scallion Plants Manurung, Putra; Tarore, Dantje; Paat, Frangky J.; Watung, Jackson F.
Jurnal Agroekoteknologi Terapan (Applied Agroecotechnology Journal) Vol. 7 No. 1 (2026): ISSUE JANUARY-JUNE 2026
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v7i1.52173

Abstract

Spodoptera exigua, a member of the Lepidoptera order and the Noctuidae family, has the ability to rapidly attack leek plants (Allium fistulosum L.). The goal of this study was to use B. bassiana on A. fistulosum to determine the death rate of S. exigua larvae. A completely randomized design (CRD) was used, with four treatments: P0 (control), P1 (conidia concentration 106/ml), P2 (conidia concentration 107/ml), and P3 (conidia concentration 108/ml). Repeat four times. Each replication consisted of ten larvae. A total of 160 S. exigua larvae were required. The observation findings showed that each concentration had a different LT50 value. Based on the LT50 data, treatment with a concentration of 108/ml effectively suppressed 50% of the larvae within 4 days. Significant temporal differences were detected for each conidia concentration. On the fourth day after application, a high mortality rate was observed in treatment P3 (conidia concentration of 108/ml), indicating a consistent daily larval mortality rate. The attack intensity reached 30% in just four days with a conidia concentration of 108/ml (P3), followed by P2 (107/ml) and P1 (106/ml). The mortality rate of S. exigua larvae increases linearly with conidia concentration. Keywords: Spodoptera exigua, Beauveria bassiana, Allium fistulosum L.   Abstrak. Spodoptera exigua tergolong ordo Lepidoptera, famili Noctuidae. S. exigua dapat menyerang tanaman tanaman bawang daun (Allium fistulosum L.) dengan cepat dalam waktu yang singkat. Tujuan penelitian ini adalah aplikasi B. bassiana pada A. fistulosum untuk mendapatkan tingkat mortalitas larva S. exigua. Completely randomized design (RAL) 4 perlakuan, terdiri atas: P0 (control), P1 (konsentrasi konidia 106/ml), P2 (konsentrasi konidia 107/ml), dan P3 (konsentrasi konidia 108/ml). Ulangan empat kali. Sampel disetiap ulangan terdiri atas 10 larvae. Total larvae yang diperlukan yaitu sebanyak 160 larva S. exigua. Hasil pengamatan mengindikasikan bahwa setiap konsentrasi memiliki nilai LT50 yang bervariasi. Dari data LT50 yang diperoleh, perlakuan dengan konsentrasi 108/ml sangat cepat mengendalikan 50 persen larvae dalam waktu 4 hari. Perbedaan waktu signifikan disetiap konsentrasi konidia yang diterapkan. Pada hari keempat setelah aplikasi, tingkat mortalitas yang signifikan terjadi pada perlakuan P3 (konsentrasi konidia 108/ml), menunjukkan tingkat mortalitas larva yang stabil setiap harinya. Intensitas serangan mencapai 30% hanya dalam empat hari dengan penggunaan konsentrasi konidia 108/ml (P3), selanjutnya P2 (konidia 107/ml), dan P1 (konidia 106/ml). Semakin tinggi konsentrasi konidia linier terhadap mortalitas level larva S. exigua. Kata kunci: Spodoptera exigua, Beauveria bassiana, Allium fistulosum L.
Percentage of Attacks and Intensity of Damage by Emerald Beetle Pest Agrilus sp. (Coleoptera: Buprestidae) on Clove Plantations in Pinolosian District, South Bolaang Mongondow Regency Watung, Jackson; Umboh, Ulrich; Tarore, Dantje; Dumalang, Sherlij
Jurnal Agroekoteknologi Terapan (Applied Agroecotechnology Journal) Vol. 6 No. 2 (2025): ISSUE JULY-DECEMBER 2025
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v6i2.64520

Abstract

Clove (Syzygium aromaticum (L) Merr.& Perry) is an aromatic shrub. In 2007, a stem-boring beetle, namely the emerald beetle Agrilus sp. (Coleoptera: Buprestidae), was found in Pinolosian Subdistrict, South Bolaang Mongondow Regency. This study aims to determine the intensity of damage and the percentage of Agrilus sp. pest infestation in Pinolosian Subdistrict, South Bolaang Mongondow Regency. The study was conducted in Pinolosian District, South Bolaang Mongondow Regency, North Sulawesi Province, from February to July 2025. The study employed a quantitative descriptive method. Data on the percentage of infestation and damage intensity caused by Agrilus sp. were processed using Microsoft Excel and presented in tables and diagrams. The highest percentage of infestation was found at Station III at 70%, The highest attack percentage was found at Station III at 70%, and the lowest attack percentage was found at Station II at 40%. The highest damage intensity was found at Station III at 50.83% with a severe damage category, and the lowest damage intensity was found at Station II at 12.50% with a mild damage category. Keywords: Attack percentage, damage intensity, and Agrilus sp. Abstrak. Cengkeh (Syzygium aromaticum (L.) Merr. & Perry) adalah tanaman perdu beraroma. Pada tahun 2007 ditemukan kumbang penggerek batang yaitu kumbang zamrud Agrilus sp. (Coleoptera: Buprestidae) di Kecamatan Pinolosian, Kabupaten Bolaang Mongondow Selatan. Penelitian ini bertujuan untuk mengetahui intensitas kerusakan dan persentase serangan hama Agrilus sp. di Kecamatan Pinolosian, Kabupaten Bolaang Mongondow Selatan. Penelitian ini dilaksanakan di Kecamatan Pinolosian, Kabupaten Bolaang Mongondow Selatan, Provinsi Sulawesi Utara dan dilaksanakan pada bulan Februari-Juli 2025. Penelitian ini menggunakan metode deskriptif kuantitatif. Data persentase serangan dan intensitas kerusakan hama Agrilus sp. diolah menggunakan Ms. Excel dan ditampilkan dalam bentuk tabel dan diagram. Persentase serangan tertinggi terdapat pada Stasiun III yaitu 70%, dan persentase serangan terendah ditemukan pada Stasiun II yaitu 40%. Intensitas kerusakan tertinggi terdapat pada Stasiun III yaitu 50,83% dengan kategori kerusakan berat, dan intensitas kerusakan terendah ditemukan di Stasiun II yaitu 12,50% dengan kategori kerusakan ringan. Kata Kunci: Persentase serangan, Intensitas kerusakan, dan Agrilus sp
Co-Authors Alfiando A. Sasauw Andrea Wangko Arter G. Umboh Balansa, Endrile Golmen BEIVY JONATHAN KOLONDAM Betsy A.N. Pinaria Chika C. Maarebia Christina Salaki Devita Gabai Emmy Senewe Fahrisal, Fahrisal Frangky J. Paat James B. Kaligis Jeane Krisen Jefri Sembiring Jenny E.A. Kandou Jimmy Rimbing Juliet M.E. Mamahit Juliet M.E. Mamahit Jusuf Manueke Jusuf Manueke Lebang, Midy San Lucia Cecilia Mandey Mamahit, Juliet Merry Eva Manurung, Putra Marco M. Supit Mareyke Moningka Margareta M. Kawulusan Maxi Lengkong Mei Lani Bandu Moulwy Dien Natasia M. Dumais Nintang T. Umboh Oddi R Pinontoan Odi R. Pinontoan Pinaria, Betsy Reity A.G. Engka Robert W Tairas Ronny Nangoi Salaki, Christina Leta Salasa, Tirsa Selvia D. Sumual Sherlij Dumalang Tambelu, Merry Feiby Threis Umboh TRINA EKAWATI TALLEI Umboh, Ulrich Washliyah, Sitti Watung, Jackson Watung, Jackson F.