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Diah Tri Widayati
Department Of Animal Breeding And Reproduction, Faculty Of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Energy Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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Journal : Buletin Peternakan

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Pengaruh Penambahan Chorionic Gonadotrophin pada Medium Maturasi terhadap Kemampuan Maturasi, Fertilisasi, dan Perkembangan Embrio secara In Vitro Kambing Peranakan Ettawa (The Effect of Chorionic Gonadotrophin Addition Into Maturation Medium on The Abili Nurvina Septi Adifa; Pudji Astuti; Diah Tri Widayati
Buletin Peternakan Vol 34, No 1 (2010): Buletin Peternakan Vol. 34 (1) Februari 2010
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v34i1.101

Abstract

This research was conducted to investigate the effect of chorionic gonadotrophin addition into maturation medium on oocyte maturation, fertilization, and embryo development in vitro of Ettawa crossbred. Oocytes were divided into 3 groups, group I: maturation medium without addition of chorionic gonadotrophin (0), group II: 10 μl/10 ml chorionic gonadotrophin was added into maturation medium (1), group III: 20 μl/10 ml chorionic gonadotrophin was added into maturation medium (2). Oocytes were transferred into 50 μl maturation medium, then covered by mineral oil. Oocyte was incubated at 39oC, 5% CO2, 95% humidity for 24 hours for maturation. Matured oocytes were inseminated with frozen semen–thawed concentration 12.5 x 106/ml. Process of fertilization were carried out on incubator 39oC, 5% CO2, 95% humidity for 5 hours. The fertilized oocytes were transferred into 50 μl drop G–1, then incubated at 39oC, 5% CO2, 95% humidity. Embryo development was monitored every 24 hours. Culture medium was changed every 48 hours. G–2 medium used second day after culture. The variables measured involved oocyte maturation, fertilization, and in vitro cleavage rate. The data were analyzed by chi–square, using SPSS 15.0 program. The result showed no significant difference on the percentage of mature oocytes and fertilization rate were 78.0%, 72.8%, 75.0% and 76.6%, 74.5%, 77.8% respectively. But cleavage rate showed significant difference (P≤0.05) withthe values of 40.8%, 11.4%, and 12.2% respectively. Based on the result it could be concluded that chorionic gonadotrophin addition into maturation medium had not increased ettawa crossbred oocytes maturation, fertilization, and in vitro cleavage rate. The best maturation, fertilization, and in vitro cleavage rate were found using maturation medium without any addition of chorionic gonadotrophin.(Key words: Does oocyte, Chorionic gonadotrophin, In vitro maturation, In vitro fertilization, In vitro embryo development)
Kinerja Reproduksi Ternak Kuda Kerja di Kabupaten Bantul, Daerah Istimewa Yogyakarta (Reproductive Performances of Working Mare at Bantul Regency, Yogyakarta Special Province) Arif Setyobudi; Kustono (Kustono); Diah Tri Widayati
Buletin Peternakan Vol 33, No 3 (2009): Buletin Peternakan Vol. 33 (3) Oktober 2009
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v33i3.110

Abstract

The experiment was conducted to determine reproductive performances of working mare at Bantul Regency, Yogyakarta Special Province. One hundred and fourten mares were used in the experiment coming from 94 respondens.Samples were collected from district of Sewon, Banguntapan and Pleret. The parameters observed were first mating age, service per conception (S/C), postpartum mating (PPM), foaling interval (F/I), body weight, working hours, andfeed consumption. The collected data were analyzed descriptively. The average of first mating age, service per conception (S/C), postpartum mating (PPM), foaling interval (FI) were 31.42±4.62 months, 2.85±0,81; 94.69±32.42days; 493.65 ± 43.01 days (ranged in 1-5 S/C; 35-180 days; 395-594 days); body weight, working hours, and feed consumption were 273.65±43.64 kg, 8 hours 11 minutes/day, 16.02±2.17 kg respectively. The conclusion of theexperiment were that the reproductive performances of working horse at Bantul Regency in Daerah Istimewa Yogyakarta is generally less than the normal performances reproductive of horse.(Key words: Reproduction performance, Working horse, Bantul Regency)
KARAKTERISTIK DAN ORGAN REPRODUKSI BETINA KANGURU POHON KELABU (Dendrolagus inustus) DI PAPUA Johan Fredrik Koibur; Kustono (Kustono); Diah Tri Widayati
Buletin Peternakan Vol 35, No 1 (2011): Buletin Peternakan Vol. 35 (1) Februari 2011
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v35i1.586

Abstract

Lima ekor betina Kanguru Pohon Kelabu (Dendrologus inustus) dewasa dengan rerata umur 1 s/d 2,5 tahun, bobot badan 10,83 kg dan panjang tubuh 72,8 cm, dibedah memakai peralatan bedah dan diambil organ reproduksinya. Karakteristik organ reproduksi didokumentasikan dengan kamera digital, diukur dan ditimbang. Penelitian ini menggunakan metode studi eksperimen deskriptif dengan teknik studi kasus. Analisis data menggunakan statistik deskriptif dan korelasi bagi beberapa informasi yang dibutuhkan lalu ditampilkan dalam nilai rerata dan standar deviasi (SD). Hasil pengamatan diperoleh panjang organ reproduksi betina D. inustus berkisar 18,88±0,78 cm (kiri) dan 19,00±0,78 cm (kanan) dengan panjang ovarium berkisar 1cm (kiri/kanan) dengan berat berkisar 0,59±0,85 g (kiri) dan 1g (kanan). Diameter dan panjang tuba fallopi, cornu uteri, corpus uteri, dan serviks masing-masing adalah: 0,57±0,01 mm (kiri) dan 0,60±0,01 mm (kanan), dan 3,00 (kiri) dan 3,26±0,18 cm (kanan); 0,54±0,02 mm (kiri) dan 0,56±0,02 mm (kanan), dan 4cm (kiri) dan 4,8±0,45 cm (kanan); 1,16±0,09 mm (kiri) dan 1,3±0,12 mm (kanan), dan 1cm; 0,3mm (kiri/kanan) dan 3cm (kiri/kanan), sedangkan panjang vagina dan vulva berturut-turut adalah 4 dan 2 cm. Hasil studi menunjukkan bahwa bentuk dan model organ reproduksi D. inustus menyerupai organ betina mamalia. 
Profil Hormon Progesteron dan Estrogen pada Kambing Peranakan Etawah yang Disinkronisasi Estrus dengan Implan Controlled Internal Drug Release Sunendar (Sunendar); Diah Tri Widayati; Aris Junaidi
Buletin Peternakan Vol 32, No 1 (2008): Buletin Peternakan Vol. 32 (1) Februari 2008
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v32i1.1242

Abstract

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Pengaruh Ukuran Folikel terhadap Qualitas Oosit Sapi Peranakan Ongole dan Kemampuan Maturasi In Vitro Diah Tri Widayati
Buletin Peternakan Vol 23, No 3 (1999): Buletin Peternakan Vol. 23 (3) Agustus 1999
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v23i3.1641

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Pengaruh Lama Pemberian Pakan Tambahan pada Masa Kering terhadap Produksi, Kualitas Susu dan Post Partum Estrus Kambing Peranakan Etawah Yustina Yuni Surandiyah; Diah Tri Widayati; Nurliyani (Nurliyani)
Buletin Peternakan Vol 21, No 1 (1997): Buletin Peternakan Vol. 21 (1) April 1997
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v21i1.1670

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Karakteristik Semen Domba pada Bulan-Bulan yang Berbeda Diah Tri Widayati
Buletin Peternakan Vol 19, No 1 (1995): Buletin Peternakan Vol. 19 (1) Juni 1995
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v19i1.1706

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PENGARUH DOSIS SPERMA YANG DIENCERKAN DENGAN NaCl FISIOLOGIS TERHADAP FERTILITAS TELUR PADA INSEMINASI BUATAN AYAM KAMPUNG Widya Asmarawati; Kustono (Kustono); Diah Tri Widayati; Sigit Bintara; Ismaya (Ismaya)
Buletin Peternakan Vol 37, No 1 (2013): Buletin Peternakan Vol. 37 (1) Februari 2013
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v37i1.1952

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh dosis sperma untuk Inseminasi Buatan (IB) pada ayam kampung terhadap fertilitas telurnya. Dosis IB ini berguna untuk mengetahui perbandingan jantan dan betina yang paling efisien dalam suatu pemeliharaan. Sperma ditampung dan dicampur dari 5 ekor ayam Pelung jantan yang berumur sekitar 52 minggu. Sperma yang telah diketahui konsentrasinya kemudian dibagi menjadi 3 bagian dan masing-masing diencerkan dengan NaCl fisiologis sehingga diperoleh dosis akhir yaitu 25x106/0,1 ml (dosis 1); 75x106/0,1 ml (dosis 2); dan 150x106/0,1 ml (dosis 3). Sembilan ekor ayam kampung betina diinseminasi untuk mengetahui pengaruh dosis terhadap fertilitas telur. Ayam betina yang digunakan adalah ayam kampung yang berumur sekitar 30 minggu. Hasil penelitian menunjukkan bahwa perlakuan dosis sperma tidak berpengaruh pada fertilitas telur ayam kampung. Fertilitas telur untuk masing-masing perlakuan dosis 1, 2, dan 3 adalah 44,13±20,84; 66,67±23,57; dan 58,75±14,36%. Kesimpulan dari penelitian ini adalah dosis sperma tidak berpengaruh pada fertilitas telur ayam kampung.(Kata kunci: Ayam kampung, Dosis sperma, Inseminasi buatan)
PENGARUH SUPLEMENTASI FETAL CALF SERUM TERHADAP KEMAMPUAN MATURASI IN VITRO OOSIT SAPI Denvy Meidian Daoed; Nono Ngadiyono; Diah Tri Widayati
Buletin Peternakan Vol 37, No 3 (2013): BULETIN PETERNAKAN VOL. 37 (3) OKTOBER 2013
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v37i3.3077

Abstract

Penelitian ini memanfaatkan hasil samping rumah potong hewan (RPH) sebagai sumber oosit untuk in vitro fertilization (IVF). Untuk meningkatkan keberhasilan IVF dilakukan suplementasi fetal calf serum (FCS) pada medium maturasi in vitro. Ovarium sapi dari RPH dibawa ke laboratorium dalam medium NaCl 0,9% pada suhu 31-34ºC. Selanjutnya oosit diaspirasi menggunakan syringe 3 ml dan jarum 23 G yang berisi Dulbeco’s-Phosphate Buffer Saline (DPBS), kemudian dimaturasikan pada inkubator CO2 modifikasi dalam medium Tissue Culture Medium-199 (TCM-199) (CO2 5%, kelembaban 99% dan suhu 37-39ºC). Oosit dibagi menjadi 2 kelompok yaitu kelompok kontrol (TCM-199) dan kelompok perlakuan (TCM-199 + 10% FCS). Angka maturasi dianalisa dengan Chi-Square, sedangkan kualitas oosit dianalisis secara deskriptif. Maturasi oosit sapi pada kelompok perlakuan berbeda nyata (P≤0,05) dibandingkan kelompok kontrol (55,22% vs 40,09%). Penggunaan 10% FCS pada medium maturasi dapat menghasilkan kualitas oosit matur yang lebih baik dibandingkan kelompok kontrol. Kesimpulan penelitian ini adalah penggunaan 10% FCS suplementasi dapat meningkatkan kemampuan maturasi oosit sapi in vitro.(Kata kunci: Fetal calf serum, Kultur oosit, Maturasi in vitro, Oosit sapi)
Suplementasi Hormon Gonadotropin Pada Medium Maturasi In Vitro Untuk Meningkatkan Perkembangan Embrio Stadium 4 Sel Kambing Bligon Dewi Pranatasari; Kustono Kustono; Diah Tri Widayati
Buletin Peternakan Vol 40, No 2 (2016): BULETIN PETERNAKAN VOL. 40 (2) JUNI 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v40i2.9080

Abstract

The study was carried out to investigate the effect of gonadotropin hormone supplementation into in vitro maturation medium on maturation, fertilization and embryo development of Bligon goats. This research steps consist of oocyte collection, in vitro maturation, in vitro fertilization, and in vitro embryo development. At the maturation stage the oocyte that had been collected and divided into two groups based on the maturation medium, that was tissue culture medium (TCM) with supplementation of GnRH 0 IU/mL and GnRH 25 IU/mL. Oocyte and embryo morphology data were analyzed descriptively. Maturation rate and embryo development data were analyzed by using independent sample t-test. Fertilization data was analyzed descriptively. The result showed the percentages of mature oocytes from gonadotropin supplementation of 0 IU/mL and 25 IU/mL were 54.10±25.97 and 54.89±26.44%, respectively. Expansion cumulus cells surrounding the oocytes might indicated the mature oocytes. Cleavage rate of the 2 cells stage were 13,02±11,09 and 27,01±16,65%; respectively, and for the 4 cells stage were 10,16±10,01% and 16,67±14.91%. Embryos obtained from the treatment, indicated uniform of blastomeres in the size, tight, compact, intact, and round-spherical shape. It could be concluded that supplementation of gonadotropin hormone into in vitro maturation medium could not increase the rate of oocyte maturation and 4 cell embryo development, but it could increase 2 cell embryo development of Bligon goats. Hormone supplementation could improved the maturation and embryo quality.
Co-Authors Ahmad Pramono Aji, Riyan Nugroho Akhmad Fathoni Andi, Megawati Andriyani Astuti Arif Setyobudi Aris Junaidi Asmarani Kusumawati Atmoko, Bayu Andri Bambang Sugito Bambang Sugito Budi Prasetyo Widyobroto Cuk Tri Noviandi Devita Hery Fatmawati Devita Hery Fatmawati Dewi Pranatasari Diatmono, Dio Fico Felsidan Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwiviyanti, Elisa Elisa Dwiviyanti Endang Baliarti Freitas, Joana da Costa Gustan Pari Gustan Pari Hari Hartadi I Gede Suparta Budisatria Ikhwan Firhamsah Ismaya (Ismaya) Ismaya Ismaya Ismaya Ismaya Jaswadi J Jaswadi J Johan F. Koibur Joko Riyanto Katsuhiro Fukuta Katsuhiro Fukuta Kresno Suharto Kumala, Seraphina Kustono (Kustono) Kustono (Kustono) Kustono K Kustono K Kustono Kustono Kustono Kustono Laelatul Rahmah Lies Mira Yusiati Marcella Arka Paramita Masato Yoshida Masato Yoshida, Masato Maulana, Hamdani Mujtahidah Anggriani Ummul Muzayyanah Mukhamad Khusnudin Nono Ngadiyono Nono Ngadiyono Nony Ariesta Nony Ariesta Nurliyani (Nurliyani) Nurvina Septi Adifa P P Putro, P P Padmawati, Fransisca Gani Paramita, Marcella Arka Paramita, Stefani Winda PUDJI ASTUTI Rahmah, Laelatul Ratri Ratna Dewi Ratri Ratna Dewi Riyan Nugroho Aji Seraphina Kumala Sigit Bintara Sitaresmi, Pradita Iustitia Sumadi Sumadi Sunendar (Sunendar) Sunendar - Tety Hartatik Tri Wahyu Pangestiningsih Trisakti Haryadi Wahyuningsih w Wahyuningsih w Widya Asmarawati Yanuar Achadri Yustina Yuni Surandiyah