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Bayu Triwibowo
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Social Sciences Other

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Journal : International Journal of Renewable Energy Development

 1 
Comparative Study on the Various Hydrolysis and Fermentation Methods of Chlorella vulgaris Biomass for the Production of Bioethanol Megawati, Megawati; Bahlawan, Zuhriyan Ash Shiddieqy; Damayanti, Astrilia; Putri, Radenrara Dewi Artanti; Triwibowo, Bayu; Prasetiawan, Haniif
International Journal of Renewable Energy Development Vol 11, No 2 (2022): May 2022
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.2022.41696

Abstract

One of the microalgae that can be potentially used to produce bioethanol is Chlorella vulgaris, as it is rich in carbohydrates. However, the carbohydrates in C. vulgaris cannot be converted directly into ethanol. This study aimed to investigate the chemical and enzymatic hydrolysis of C. vulgaris, which is subsequently followed by fermentation. The catalysts used in the chemical hydrolysis were hydrochloric acid, sodium hydroxide, and potassium hydroxide, while the enzymes used were the mixture of alpha-amylase + glucoamylase, alpha-amylase + cellulase, and alpha-amylase + glucoamylase + cellulase. The hydrolysate obtained from chemical hydrolysis was fermented through Separate Hydrolysis Fermentation (SHF), while the one from enzymatic hydrolysis was fermented through Simultaneous Saccharification and Fermentation (SSF), in which both processes used S. cerevisiae. After undergoing five hours of enzymatic hydrolysis (using alpha-amylase + glucoamylase), the maximum glucose concentration obtained was 9.24 ± 0.240 g/L or yield of 81.39%.  At the same time and conditions of the substrate on chemical hydrolysis, glucose concentration was obtained up to 9.23 + 0.218 g/L with a yield of 73.39% using 1 M hydrochloric acid. These results indicate that chemical hydrolysis is less effective compared to enzymatic hydrolysis. Furthermore, after 48 hours of fermentation, the ethanol produced from SHF and SSF fermentation methods were 4.42 and 4.67 g/L, respectively, implying that producing bioethanol using the SSF is more effective than the SHF method.
Co-Authors A Halim Abdul Halim Abdul Halim Achmad Chafidz Ahmed Tessario Ekanuramanta Aldiansar , Divifan Armyko Alfiansyah Aji Pratama Amin Retnoningsih Amrullah, Fauzan Anggun Septiamurti Annie Mufyda Rahmatika, Annie Mufyda Asnafi, Agus Astrilia Damayanti Bahlawan, Zuhriyan Ash Shiddieqy Cahyani, Novia Noor Catur Rini Widyastuti, Catur Rini Chafidz, Achmad Dewi Artanti Putri Dewi Selvia Fardhyanti Dhoni Hartanto Dody Herdian Saputra Riyadi Dody Herdian Saputra Riyadi Dwi Prasojo Dyta Silvia Mayasari Fauzan Amrullah Fauzan Amrulloh Fauziah, Whilda Fidyawati, F Gicha Maria Rahayu Hadiyanti, Luthfia Nur Haniif Prasetiawan Harianingsih, Harianingsih Heni Pujiastuti Hidayah, Uswatun Nur Kevin Thomas Kusuma, Andre Dianata Hogi Laraswati, Vivi Anggraeni Loveyanto, Rendy Okta Maharani Kusumaningrum Maksiola, Masni Mayasari, Dyta Silvia Megawati Megawati - Megawati Megawati Megawati Megawati Megawati Megawati Megawati Megawati Mezaki, Naufal Mudrik Miftahuddin Azhari Muhammad Fikri Al Ghifari Mutaqin, Afrizal Mai Ngabiyanto - Nina Hartini Nina Hartini Nur Qudus Nurazizah, Nilasari Nurul Huda Nuryoto Nuryoto Paramita, Oktavianti Pradana, Daffa Zamiira Pradiza, Zefanya Pradnya, Irene Nindita Prakasita, Iffat Ganjar Fadhila Prasojo, Dwi Pratama, Alfiansyah Aji Prayoga, Ade Bintang Prima Astuti Handayani Puspita, Dessy Ratna Putri, Dwiana Asmara Putri, Raden Rara Dewi Artanti Radenrara Dewi Artanti Putri, Radenrara Dewi Artanti Rahardjo, Teguh Hardi Ratna Dewi Kusumaningtyas Reshita Amalia Ramadhani Reshita Amalia Ramadhani Ria Wulansarie, Ria Rizki, Angela Dita Salwa, Dian Fatimatus Sandra Anggita Fadriana Sa’adah, Nur Septiamurti , Anggun Sinta Andriyani Solichin Solichin Syarifatur Richana Syarifatur Richana Syifa Karimah Trianita Satyawati Nabilah Wara Dyah Pita Rengga Zefanya Pradiza Zuhriyan Ash Siddieqy Bahlawa